What is New in the World of

Microscopy py

Enrique Changq gAgilent Technologies, Inc.MSSU

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What is New in the World of Microscopy?

Scientists are constantly pushing the frontiers of micro measurement.

Microscopy is being driven to a new level of application requirements which include multifunction measurements and/or observations.

There are numerous advances in the science of microscopy requiring more sophisticated measurements tool.

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What is a Digital Microscope?

An instrument that behaves like a conventional microscope but with specific advantages based on p p gdefined applications.

It is completely integrated in most cases with an Imaging System so both the microscope and the Imaging System work in unison.

With Di it l Mi th d iWith a Digital Microscope other advances in microscopy techniques can be easily added at a later time.

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Who Would Reap the Benefits of Using a Digital Microscope?Microscope?

Scientists who want to perform repetitive tasks with a p phigh degree of precision and reliability (i.e. High Throughput Screening, repetitive observations)

Applications that require more than one detector (i eApplications that require more than one detector (i.e. camera, photodiode, PMT, etc.)

Microscopy core facilities with a user group requiring aMicroscopy core facilities with a user group requiring a wide range of microscopy applications (i.e. FISH, TIRF, FRET)

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iMICiMICAgilent Technologies introducesAgilent Technologies introduces the iMIC Digital Microscope

A unique inverted microscope q pplatform that allows multifunction applications within the click of a buttonbutton

The microscope is computer driven and uses a CDD camera asdriven and uses a CDD camera as its viewing and image capturing method

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Integration!

Integration, Integration, Integration (the name of the game)(the name of the game)

• Allows multi entry ports and multi exit portexit port

• Multiple applications in the same platform

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iMIC Components

Motorized Objective Changer Octagonal filter cube to direct beam path

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Motorized focusing both coarse and fine Motorized filter slider

Objective.avi

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Filter.avi

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iMICCan accomodate multiple entry ports (excitation

iMICentry ports (excitation source) and multiple detector ports (emission). Providing a versatile range of applications within the same platform

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Microscopy Techniques

The iMIC accomplishes common light microscopy techniques including:

• Transmitted light Microscopy• Dark Phase• Wide Field Fluorescence• Wide Field Fluorescence

The iMIC can also accommodate less conventional techniques

• Laser scanning for Photobleaching or Photoexcitation• Confocal Scanning• TIRF

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Motorized XY Scanning Stage

In addition to a single slide holder there is the option of a larger sample stage that accommodates petri dishes as well as microtiter plates

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Other Specimen Holders

Custom made holders can be design for optimal end user applicationsoptimal end user applications

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Environmental Control

Optional Environmental Chamber

• Controls Temperature, Humidity and CO2

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Fluorescence Energy Transfer FRET

A popular microscopy technique thatA popular microscopy technique that quantitates the amount of energy between molecules hence providing a ratio of the efficiency betweena ratio of the efficiency between molecules of interest

The iMIC has an optional completely integrated FRET system that easily engages and disengages.

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TIRF

TIRF uses oblique laser light that allows to the detect single molecule fluorescence in the top surface of a g plabeled sample

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Converting a Traditional Microscope into a TIRF detection systemdetection systemInserting a TIRF epifluorescent condenser into the back of a microscopep

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Photobleaching and Photoexcitation

A laser light is used to either photobleach a fluorochrome and measure its recovery or photoexcite and measure the dynamics y p yof the photoexcited dye.

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Scanhead for laser scanning into the iMIC

Yanus-2

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Yanus-2 XY Scanning

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Integration

A Digital Microscope can integrate all of the previously discussed techniques into a single platform

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Questions?Questions?

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