What is SV40 T-antigen?

What does it do?

Presented by 李柏毅Genome Engineering Laboratory,

Institute of Bioinformatics,National Yang-Ming University

2003/12/12

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Simian Virus 40

Hosts: monkeys and mice DNA virus with 5,227 base pairs

Origin

Early mRNAs Late mRNAs

5’ 5’

SV40

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SV40 T-antigen

AUG UUA UUAEarly pre-mRNA

Large T mRNA

Small T mRNA

AAAn

AAAn

Large T 94kd proteinSmall T 17kd protein

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Large T-antigen

Delete 1-126 a.a. Delete 136-C terminus Addition 127-132 a.a to

β-galactosidase and pyruvate kinase

Delete 127-132 a.a.

Immunofluorescence microscopy

Daniel Kalderon, Bruce L. Roberts, Alan E. Smith, A Short Amino Acid Sequence Able to Specify Nuclear Location, Cell 39:499-509, 1984

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Tumor Virus

Human cells are semipermissive cell

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Tumor Virus

SV40 Large T

Ad5 E1A 13S

Ad5 E1A 12S

Ad5 E1B 55K

Rb-binding P53-binding

Release transcription factor for Rb

Protein binding sites

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THANKS!

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Hahn WC, et al,. Creation of human tumor cells with defined genetic elements. Nature 400, 464–468 (1999)