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Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

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Page 1: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)
Page 2: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Welcome toMolecular Biology Through Discovery

Thursday, 20 September 2012DNA Structure / Benzer (1959)

Page 3: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

What would you like discussed in class, on any subject that has already come up?

General Questions

Can you clarify what we are using the mentors for again? I want to make sure that when I send them an email I can tell them why I need a mentor.

Page 4: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)
Page 5: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA Structure

Page 6: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA Structure

Page 7: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA Structure

Page 8: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA StructureC. What are the Chargaff rules and how they were found?

Page 9: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Why is C uniformly low?Genetic Imprinting

Sapienza C (1990) Sci Am Oct 52- 60

DNA methylation

Reik W, Walter J (2001) Nature Rev 2:21-32

Dana Krempels, University of Miami

Cytosine

Page 10: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA Structure

Page 11: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA Structure

0.140.370.360.13

Page 12: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA StructureSQ7. What would you expect to happen to the diffraction pattern if the helix shown in Fig. 8B-D were squashed, i.e. the helix were more tightly wound, more like a solenoid?

Page 13: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA StructureSQ7. What would you expect to happen to the diffraction pattern if the helix shown in Fig. 8B-D were squashed, i.e. the helix were more tightly wound, more like a solenoid?

?? ?

?

Page 14: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA StructureSQ7. What would you expect to happen to the diffraction pattern if the helix shown in Fig. 8B-D were squashed, i.e. the helix were more tightly wound, more like a solenoid?

??

angle?

Page 15: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA StructureSQ7. What would you expect to happen to the diffraction pattern if the helix shown in Fig. 8B-D were squashed, i.e. the helix were more tightly wound, more like a solenoid?

Page 16: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

DNA StructureSQ7. What would you expect to happen to the diffraction pattern if the helix shown in Fig. 8B-D were squashed, i.e. the helix were more tightly wound, more like a solenoid?

?

angle?

Page 17: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)

rII region of bacteriophage T4

Page 18: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)

rII region of bacteriophage T4

mutation

Page 19: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)

rII region of bacteriophage T4

Page 20: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)Determine distances between markers by

Recombination

Page 21: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)I still have trouble connecting recombination with the distance b/w genetic markers.

Page 22: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)I still have trouble connecting recombination with the distance b/w genetic markers.

Richard Twyman, University of Warwick

Page 23: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Benzer (1959)I still have trouble connecting recombination with the distance b/w genetic markers.

Elizabeth Ellis University of Strathclyde

Page 24: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)
Page 25: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)
Page 26: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Results vs Conclusions

Shahroze Mandi Shaun

Bobby Supriya Tayab

Kaleigh Jonathan Michael Colleen

AbdulCailin Sue Kristen

Abdallah Celeste Neda Yordanos

Me

Page 27: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Coming Attractions

Page 28: Welcome to Molecular Biology Through Discovery Thursday, 20 September 2012 DNA Structure / Benzer (1959)

Goodbye fromMolecular Biology Through Discovery

Thursday, 20 September 2012