Supplementary Material

Antimicrobial characterization of erythorbyl laurate

for practical applications in food and cosmetics

Jin-Woo Kim a,1, Hyunjong Yu a,1, Kyung-Min Parkb,1, Pahn-Shick Changa,c,d*

aDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826,

Republic of Korea

bDepartment of Food Science and Biotechnology, Wonkwang University, Iksan 54538,

Republic of Korea

cCenter for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of

Korea

dResearch Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826,

Republic of Korea

*Author to whom correspondence should be addressed [telephone: +82 2 880 4852; fax: +82

2 873 5095; e-mail: pschang@snu.ac.kr]

1 Equal contribution as first authors.

This document file contains supplementary data (Table S1, Figure S1, S2 and S3).

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Table S1: Pathogens used in the susceptibility tests

Classification Related source Pathogen Hazard

Yeast Infectious skin disease C. albicans KCTC 7678 Candidiasis

Mold

Infectious skin disease T. mentagrophytes ATCC 18748 Tinea pedis

Food-borne diseaseA. nidulans ATCC 10074 Chronic granulomatous

R. oryzae ATCC 10404 Mucormycosis

BacteriaInfectious skin disease

P. acnes ATCC 6919 Acne vulgaris

S. pyogenes ATCC 19615 Impetigo

Food-borne disease C. perfringens ATCC 13124 Perfringens food poisoning

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Figure S1: Effect of pH on the protonation of erythorbyl laurate. The percentage of

protonated erythorbyl laurate was 22.03, 2.75 and 0.28% at pH 5.0, 6.0, and 7.0, respectively.

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Figure S2: Structure change of erythorbyl laurate by oxidation in alkaline solution.

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(a) (b)

(c) (d)

Figure S3: Bactericidal activities of erythorbyl laurate in O/W

emulsions against Gram-positive and –negative bacteria at

concentrations of ① 5.0, ② 4.0, ③ 3.0, ④ 2.0, ⑤ 1.0, ⑥ 0.5, and ⑦ negative

(without erythorbyl laurate) and ⑧ positive control (500 ppm ampicillin): (a) S.

aureus ATCC 12692, (b) L. monocytogenes ATCC 19115, (c) E. coli ATCC 35150, and

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(d) P. aeruginosa ATCC 15692.

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