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[email protected] Tel: +46-70-7171032 www.scrr.se Swedish Centre for Resource Recovery Ways2Taste: Integrating fungi into industrial processes Mohammad Taherzadeh Project leader Professor in bioprocess technology Swedish Centre for Resource Recovery (SCRR) University of Borås Sweden

Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

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Page 1: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Ways2Taste:Integrating fungi into industrial

processes

Mohammad TaherzadehProject leader

Professor in bioprocess technologySwedish Centre for Resource Recovery (SCRR)

University of BoråsSweden

Page 2: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Resource Recovery in Nature?

Page 3: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Recycling in nature

Resources

Human activity

Wastes/CO2

Nature

Nature

Algae…

Fungi

Insects

Plants

Bacteria

Page 4: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Recycling in nature

Resources

Human activity

Wastes/CO2

Nature

Nature

Algae…

Fungi

Insects

Plants

Bacteria

Page 5: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Recycling in nature

Resources

Human activity

Wastes/CO2

Nature

Nature

Algae…

Fungi

Insects

Plants

Bacteria

Page 6: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Global emissions of CO2 (1751-2015)

Source: WRI

Page 7: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

CO2 rises in the atmosphere!

Page 8: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

… even industrial wastes….

Bagasse from sugarcane(~ 500 million tons/year)

Palm oil residuals(~ 50 millon tons/year)

Page 9: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Recycling in nature (our goal is to make faster recycling)

Resources

Human activity

Wastes/CO2

Nature

Nature

Algae…

Fungi

Insects

Plants

Bacteria

Page 10: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungi

Page 11: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungi (micro or macro)?

Page 12: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungi grow on solid or in liquid

Page 13: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungicultivation

Fumaric acidLactic acid

Ethanol

Amylases

CellulasesPectinasesProteasesSteroid 11 α-hydroxylasesPhytasesTannasesUreasesLipases

Lipids

Chitosan/chitinAnimal/Fish feed

Amino acids

Metabolites

Enzymes

BiomassHexosesPentosesMonomers

Sweet potato starchBarley

CornPure starchRice

Cassava

CorncobCassava pulpManure fibreCorn strawWoodCelluloseChitinXylanWheat strawCellulose

Orange peel Citrus pectinRice bran

Wheat bran

Raw Materials Hydrolysis

Cranberry pomace

Cotton residuesHazelnut shellsRice branCoconut oil cakeSesame oil cake

Pectin

LactoseSucrose

Capabilites of the fungi

Immobilizingmicroorganisms

Other products

Pigments

Human food

Starch wastewater

Page 14: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungicultivation

Lactic acid

Ethanol

Amylases

CellulasesPectinases

Lipids

Chitosan/chitinAnimal/Fish feed

Metabolites

Enzymes

BiomassHexosesPentosesMonomers

Pure starch

WoodCelluloseXylan

Wheat strawOrange peel Citrus pectin

Wheat bran

Raw Materials Hydrolysis

Cotton residues

Pectin

LactoseSucrose

Our works with fungi since 1999

Immobilizingyeast

Other products

Pigments

Human food

Starch wastewater

Proteases

Phytases

Lipases

Page 15: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Can we use fungi to stimulate circular economy and convert our residuals to value-added

products?

Page 16: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Can fungi help in developing biorefinery from ethanol plants?

Fungi bioreactors

Ethanol

Fungal biomass(for feed or food)Lignocelluloses

Fermentable clean residuals(protein, starch, sugars, etc.in e.g. thin stillage)

PretreatmentsPigments

Biopolymers

Other products

Page 17: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Major research and development questions?

• Fungi that can:– Major product? Ethanol?– Consume pentoses and hexoses?– Produce hydrolytic enzymes?– Can grow on different residuals streams?

• Straw, bran, saw dust, stillage, starch, crème, milk,…

• Being edible:– Fungal biomass as feed or food?

• Morphology• Pretreatment of materials before fungi?

Page 18: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

What is Ways2Taste• Collaborative project• Developing innovations in the region• Smart and sustainable growth• Biotechnology use of edible filamentous

fungus• Period: Sep. 2018 until Dec. 2021

Page 19: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Tofu Tempe

Rhizopus

Red Rice Oncom

Monascus Neurospora

FusariumBelong to Zygomycetes

Belong to AscomycetesEdibleSafe

Aspergillus

Miso

Special focus on edible fungi

Quorn

Page 20: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungal biorefiery

Fungi biorefineries

Ethanol

Fungal biomass(for feed or food)Lignocelluloses

& wastes

Fermentable clean residuals(protein, starch, sugars, etc.in e.g. thin stillage)

PretreatmentsPigments

Other products

Page 21: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungal biorefiery

Fungi biorefineries

Ethanol

Fungal biomass(for feed or food)

Residuals from:AgricultureBeer Ice creamFisheryBreadMunicipal food wastes……..

Pigments

Other products

Page 22: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungal biorefiery

Fungi biorefineries

EthanolFood Feed

Residuals from:AgricultureBeer Ice creamFisheryBreadMunicipal food wastes……..

Page 23: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Fungal biorefiery

Fungi biorefineries:

Small& Large

EthanolFood Feed

Residuals from:AgricultureBeer Ice creamFisheryBreadMunicipal food wastes……..

Page 24: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Projekt partners• Collaborating partners:

– Högskolan i Borås– Chalmers– Innovatum

• Funding:– EU Regional fund (Via Tillväxtverket)– Högskolan i Borås– Lantmännen – FOV– Steinbrenner & Nyberg– Raw Food i Borås

• Target groups:– Companies as the primary target group– Other companies as a secondary target group

Page 25: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Welcome to the first workshop of Ways2Taste

Page 26: Ways2Taste: Integrating fungi into industrial processes · • Fungi that can: – Major product? Ethanol? – Consume pentoses and hexoses? – Produce hydrolytic enzymes? – Can

[email protected]: +46-70-7171032

www.scrr.seSwedish Centre for Resource Recovery

Thank you!