Waters Source Pressure Test Unit Operator’s Guide · Contact Waters ® with enhancement requests or technical quest ions regarding the use ... Fitting the source pressure test unit

Waters Source Pressure Test Unit

Operator’s Guide

71500104602/Revision D

Copyright © Waters Corporation 2010All rights reserved

Copyright notice

© 2010 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

Trademarks

Waters is a registered trademark of Waters Corporation, and IonSABRE, LockSpray, MUX-technology, NanoFlow, Quattro Premier XE and ZSpray and “THE SCIENCE OF WHAT’S POSSIBLE.” are trademarks of Waters Corporation.Other trademarks or registered trademarks are the sole property of their respective owners.

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Customer comments

Waters’ Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.We seriously consider every customer comment we receive. You can reach us at [email protected].

Contacting Waters

Contact Waters® with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose chemical, biological, and radiological hazards. You must know the potentially hazardous effects of all substances you work with. Always follow

Waters contact information

Contacting medium InformationInternet The Waters Web site includes contact

information for Waters locations worldwide. Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800 252-HPLC, or fax 508 872 1990.For other locations worldwide, phone and fax numbers appear in the Waters Web site.

Conventional mail Waters Corporation34 Maple StreetMilford, MA 01757USA

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Good Laboratory Practice, and consult your organization’s safety representative for guidance.

Considerations specific to the Waters Source Pressure Test Unit

Solvent leakage hazard

The source pressure test unit is used with Waters mass spectrometers fitted with a ZSpray source. The tests detailed in this document are designed to ensure that no more than 10% of the eluent entering the ZSpray™ source can enter the laboratory atmosphere. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate.

Flammable solvents hazard

Never let the nitrogen supply pressure fall below 400 kPa (4 bar, 58 psi) during an analysis in which flammable solvents are used. Laser radiation hazard.

Warning: To confirm the integrity of the source exhaust system, renew the source O-rings at intervals not exceeding one year.

Warning: To prevent the ignition of accumulated solvent vapors inside the source, maintain a continuous flow of nitrogen through the source whenever significant amounts of flammable solvents are used during the instrument’s operation.

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High temperature hazard

The solvent delivery system (LC pump or syringe pump) can become hot during operation. Power off the delivery system when not in use.

High voltage hazard

Hazards associated with removing an instrument from service

Warning: To avoid burn injuries, avoid touching the source enclosure with your hand when operating or servicing the instrument.

Warning: • To avoid electric shock, do not remove the mass spectrometer’s

protective panels. The components they cover are not user-serviceable.

• To avoid nonlethal electric shock when the instrument is in Operate mode, avoid touching the areas marked with the high voltage warning symbol. To touch those areas, first put the instrument in Standby mode.

Warning: To avoid personal contamination with biohazardous or toxic materials, wear chemical-resistant gloves during all phases of instrument decontamination.

Warning: To avoid puncture injuries, handle syringes, fused silica lines, and borosilicate tips with care.

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When you remove the instrument from use to repair or dispose of it, you must decontaminate all of its vacuum areas. These are the areas in which you can expect to encounter the highest levels of contamination:

• Source interior• Waste tubing• Exhaust system• Rotary pump oil (where applicable)

The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible. That authority must also prescribe the method of decontamination to be used and the appropriate protection for personnel undertaking the decontamination process.You must handle items such as syringes, fused silica lines, and borosilicate tips used to carry sample into the source area in accordance with laboratory procedures for contaminated vessels and sharps. To avoid contamination by carcinogenic, toxic, or biohazardous substances, you must wear chemical-resistant gloves when handling or disposing of used oil.

Safety advisoriesConsult Appendix A for a comprehensive list of warning and caution advisories.

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Operating this instrument

When operating this instrument, follow standard quality-control (QC) procedures and the guidelines presented in this section.

Applicable symbols

Audience and purposeThis document is intended to be used by qualifies operators of the mass spectrometer.This document describes how to prepare for pressure testing the mass spectrometer, how to perform the pressure test and how to return the mass spectrometer to normal operation.

Intended use of the Waters Source Pressure Test UnitThe Waters Source Pressure Test Unit assesses potential gas and vapor emissions from a mass spectrometer’s source and waste system. It also helps monitor the safe operation of the instrument.

Symbol Definition

Manufacturer

Authorized representative of the European Community

Confirms that a manufactured product complies with all applicable European Community directives

Australia C-Tick EMC Compliant

Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements

Consult instructions for use

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The source pressure test unit must be used whenever an instrument’s source enclosure O-rings are replaced (this is typically performed as part of the annual preventative maintenance). The Waters Source Pressure Test Unit is for research use only.See also: The appropriate Waters mass spectrometer and source option operator’s guides.

Calibrating To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should include the entire range of QC samples, typical specimens, and atypical specimens.When calibrating mass spectrometers, consult the calibration section of the operator’s guide for the instrument you are calibrating. In cases where an overview and maintenance guide, not operator’s guide, accompanies the instrument, consult the instrument’s online Help system for calibration instructions.

Quality-controlRoutinely run three QC samples that represent subnormal, normal, and above-normal levels of a compound. Ensure that QC sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily.

ISM classification

ISM Classification: ISM Group 1 Class AThis classification has been assigned in accordance with CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class A products are suitable for use in commercial, (that is, nonresidential) locations and can be directly connected to a low voltage, power-supply network.

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EC authorized representative

Waters Corporation (Micromass UK Ltd.)Floats RoadWythenshaweManchester M23 9LZUnited Kingdom

Telephone: +44-161-946-2400Fax: +44-161-946-2480Contact: Quality manager

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Table of Contents

Copyright notice ................................................................................................... ii

Trademarks ............................................................................................................ ii

Customer comments ............................................................................................ iii

Contacting Waters ............................................................................................... iii

Safety considerations .......................................................................................... iii Considerations specific to the Waters Source Pressure Test Unit................... iv Safety advisories ................................................................................................. vi

Operating this instrument ................................................................................ vii Applicable symbols ........................................................................................... vii Audience and purpose....................................................................................... vii Intended use of the Waters Source Pressure Test Unit ................................. vii Calibrating ....................................................................................................... viii Quality-control ................................................................................................. viii

ISM classification .............................................................................................. viii ISM Classification: ISM Group 1 Class A ...................................................... viii

EC authorized representative ........................................................................... ix

1 Using the Waters Source Pressure Test Unit .................................. 1-1

When to use the source pressure test unit .................................................. 1-2

The source pressure test unit ........................................................................ 1-2

Fitting the source pressure test unit to the mass spectrometer ........... 1-2 General ............................................................................................................. 1-2 Fitting the tee-piece to the desolvation gas line ............................................ 1-3 Preparing the source exhaust line for fitting the source exhaust valve ....... 1-5

Preparing the instrument for the pressure test ........................................ 1-7 Required materials .......................................................................................... 1-7

Table of Contents xi

Performing the pressure test ......................................................................... 1-9 If the source fails the pressure test............................................................... 1-11

Returning the instrument to normal operation ...................................... 1-12 Required materials ........................................................................................ 1-12

Contacting Waters .......................................................................................... 1-13

A Safety Advisories .................................................................................. A-1

Warning symbols ............................................................................................... A-2 Task-specific hazard warnings........................................................................ A-2 Warnings that apply to this instrument......................................................... A-3

Caution symbol .................................................................................................. A-5

Warnings that apply to all Waters instruments ......................................... A-5

Electrical and handling symbols ................................................................. A-12 Electrical symbols .......................................................................................... A-12 Handling symbols .......................................................................................... A-13

Index ..................................................................................................... Index-1

xii Table of Contents

1 Using the Waters Source Pressure Test Unit

This chapter tells you when to use the source pressure test unit, how to fit it to the mass spectrometer and how to perform the pressure test.

Contents:

Topic PageWhen to use the source pressure test unit 1-2The source pressure test unit 1-2Fitting the source pressure test unit to the mass spectrometer 1-2Preparing the instrument for the pressure test 1-7Performing the pressure test 1-9Returning the instrument to normal operation 1-12Contacting Waters 1-13

1-1

When to use the source pressure test unit

The source pressure test unit is used with Waters mass spectrometers fitted with a ZSpray source.To ensure that your instrument complies with EN61010-1 regulation, you must use the source pressure test unit to verify the operation of the source when either or both these conditions apply:

• You change any of the source enclosure O-rings.• You doubt the integrity of the source and source exhaust.

Note: The pressure test is not applicable to NanoFlow™ sources.

The source pressure test unit

The source pressure test unit comprises a pressure test meter (with integral pressure relief valve), a source exhaust valve, and parts used to install the device on the mass spectrometer.The pressure test meter connects into the instrument’s desolvation gas line via a 4-mm tee-piece. When pressure testing is not being performed, the pressure test meter is removed and a blanking plug fitted in its place.

Fitting the source pressure test unit to the mass spectrometer

GeneralThe pressure test meter is supplied with a length of 4-mm flexible tube. To perform the pressure test, you must connect the tube into the instrument’s desolvation gas line via a 4-mm tee-piece. After fitting, this tee-piece is left in position during normal instrument operation (that is, when source pressure testing is not being performed).See also: “Fitting the tee-piece to the desolvation gas line” on page 1-3.

Warning: The exhaust system of a ZSpray source is a safety device and MUST be operational.

1-2 Using the Waters Source Pressure Test Unit

You must connect the source exhaust valve into the source exhaust line (not the rotary vacuum pump exhaust line) before performing the source pressure test. After performing the source pressure test, you must remove the source exhaust valve from the source exhaust line.See also: “Preparing the source exhaust line for fitting the source exhaust valve” on page 1-5, and “Returning the instrument to normal operation” on page 1-12.

Gas connections when performing the source pressure test

Fitting the tee-piece to the desolvation gas line

Required materials

• Chemical-resistant, powder-free gloves• Sharp knife

To fit the tee-piece to the desolvation gas line:

1. In the MassLynx™ Tune window, click Press for Standby, and confirm that the instrument status indicator shows red.

Warning: To avoid electric shock, ensure that the mass spectrometer is in Standby mode when commencing this procedure.

Probe adjustment flange

Source

Nebulizer gas connection

Desolvation gas connection

Mass spectrometer

Nitrogen exhaust waste bottle

Pressure test meter

Source exhaust valve

Laboratory exhaust port

4-mm tee-piece

Exhaust connection

4-mm connector

Fitting the source pressure test unit to the mass spectrometer 1-3

2. Wait 3 minutes.Rationale: Waiting 3 minutes allows the desolvation gas flow to cool the source.

3. Click to stop the desolvation gas flow.

4. Power-off the solvent delivery system (LC pump or syringe pump).

5. Open the instrument’s access door.

6. Disconnect the solvent delivery line from the probe.

7. Disconnect and remove the 4-mm flexible tubing between the instrument’s desolvation gas connection and the probe adjustment flange.

8. Use the sharp knife to cut the tubing to fit the tee-piece.

9. Connect the two cut ends of the tubing to two of the tee-piece ports.Alternative: Connect the tee-piece into new 4-mm flexible tubing of appropriate length.

10. Fit the 4-mm blanking plug to the open tee-piece port.Note: You will connect the pressure test meter to this port when preparing the instrument to perform the pressure test.See also: “Preparing the instrument for the pressure test” on page 1-7.

11. Connect the flexible tubing, with tee-piece, between the instrument’s desolvation gas connection and the probe adjustment flange. See the figure “Gas connections when performing the source pressure test” on page 1-3.

Warning: The source can be hot. To avoid burn injuries, take great care while working with the instrument’s access door open.

Warning: The solvent delivery connections can be contaminated with biohazardous and toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.

Caution: To avoid gas leaks, cut the flexible tubing squarely (that is, perpendicular to its horizontal axis).


12. Close the instrument’s access door.

Preparing the source exhaust line for fitting the source exhaust valve

Required materials

• Chemical-resistant, powder-free gloves• Sharp knife

To prepare the source exhaust line for fitting the source exhaust valve:

1. Use the sharp knife to cut the tubing connected between the nitrogen exhaust waste bottle and the laboratory exhaust port at a point approximately 50 mm (2 inches) from the bottle. See the figure “Nitrogen exhaust waste bottle” on page 1-6.

Warning: The nitrogen exhaust waste line can be contaminated with biohazardous and toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.

Caution: • To avoid damaging the instrument, you must remove the source

exhaust valve after performing the source pressure test and before resuming normal instrument operation.See also: “Returning the instrument to normal operation” on page 1-12.

• To avoid gas leaks, cut the flexible tubing squarely (that is, perpendicular to its horizontal axis).

Fitting the source pressure test unit to the mass spectrometer 1-5

Nitrogen exhaust waste bottle

2. Disconnect the short length of tubing from the nitrogen exhaust waste bottle’s outlet connection.

3. Connect the short length of tubing to one of the source exhaust valve’s ports.

4. Store the source exhaust valve and attached short length of tubing with the pressure test meter.

5. Connect the cut end of the tubing (leading to the laboratory exhaust port) to the nitrogen exhaust waste bottle’s outlet connection.

To laboratory exhaust port

From instrument exhaust connection

50 mm (2 inches)

Cut tube here


Preparing the instrument for the pressure test

Required materialsChemical-resistant, powder-free gloves

To prepare the instrument for the pressure test:

1. In the MassLynx™ Tune window, click Press for Standby, and confirm that the instrument status indicator shows red.

2. Wait 3 minutes.Rationale: Waiting 3 minutes allows the desolvation gas flow to cool the source.

3. Click to stop the desolvation gas flow.

4. Turn off the solvent delivery system (LC pump or syringe pump).

5. Open the instrument’s access door.

6. Disconnect the solvent delivery line from the probe.

7. Remove the blanking plug from the tee-piece.

Warning: To avoid electric shock, ensure that the mass spectrometer is in Standby mode when commencing this procedure.

Warning: The source can be hot. To avoid burn injuries, take great care while working with the instrument’s access door open.

Warning: The solvent delivery and nitrogen exhaust waste line connections can be contaminated with biohazardous and toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.

Preparing the instrument for the pressure test 1-7

8. Connect the pressure test meter tube to the tee-piece.

Pressure test meter connected to the tee-piece

Note: This figure shows the pressure test meter connected to a Quattro Premier XE™ mass spectrometer; the connections for other instruments are similar.

9. Disconnect the tubing leading to the laboratory exhaust port from the nitrogen exhaust waste bottle’s outlet connection.

10. Connect the short length of tubing fitted to the source exhaust valve to the nitrogen exhaust waste bottle’s outlet connection.

11. Connect the free end of the tubing leading to the laboratory exhaust port to the free connection on the source exhaust valve (so that the source exhaust valve is now connected in the line between the nitrogen exhaust waste bottle and the laboratory exhaust port). See the figure “Gas connections when performing the source pressure test” on page 1-3.

Tee-piece

Pressure test meter

Desolvation gas connection


12. Ensure that the source exhaust valve is open.

Source exhaust valve in the open position

13. Ensure that the instrument is setup for use, that it is in the correct configuration, and that the probe is fitted.See also: The appropriate instrument operator’s guide.

Performing the pressure test

To perform the pressure test:

1. On the MassLynx Tune window Source page, set Desolvation to 0 L/hr.

2. Set Cone to the appropriate value for the source/probe type to be tested, and, if appropriate, adjust the other conditions as shown in the following table.

Cone gas flow rates

Source/probe type Cone (L/h) Other conditionsESI 300APCI 300 Use appropriate fittings to

blank-off the nebulizer gas at the instrument and probe.

APCI (Quattro Premier XE)

0

Performing the pressure test 1-9

3. Switch on the pressure test meter by pressing and simultaneously.

4. Set the pressure test meter to read pressure in mbar.See also: The pressure test meter manufacturer’s documentation.

5. On the MassLynx Tune window, click to start the gas flow.

6. Wait a few seconds for the gas flow to stabilize.

7. Record the reading displayed on the pressure test meter (P1).

8. Close the source exhaust valve.

Source exhaust valve in the closed position

9. Wait a few seconds for the gas pressure to stabilize.

10. Record the reading displayed on the pressure test meter (P2).

IonSABRE™ APCI 0 Set the probe support gas control valve to less than one-third of a turn from the fully-closed position (that is, fully clockwise).

APPI Set the Cone and other conditions as for the appropriate APCI probe above.

MUX-technology™ 0LockSpray™ 300

Cone gas flow rates (Continued)

Source/probe type Cone (L/h) Other conditions


11. Open the source exhaust valve.

12. Switch off the pressure test meter by pressing .

13. On the MassLynx Tune window, click to stop the gas flow.

14. Compare the values of P1 and P2.The source is operating correctly (that is, it passes the pressure test) if P2 > 10 × P1, and P2 > 100 mbar.

15. If the source fails the pressure test, perform the following procedure.

If the source passes the pressure test, prepare the instrument for normal operation. See also: “Returning the instrument to normal operation” on page 1-12.

If the source fails the pressure testIf the source fails the pressure test, you must check both the source and the probe and then repeat the test.

To check the source and probe:

1. Ensure that the source and probe are fitted correctly.

2. Ensure that all the source and probe O-ring seals are in place.See also: The appropriate instrument operator’s guide.

3. Ensure that the source exhaust line between the instrument and the source exhaust waste valve is correctly fitted.

Warning: If the source fails the pressure test, the integrity of its waste system can be impaired; do not reconnect or start the solvent delivery system until the source passes the pressure test.

Caution: You must remove the source exhaust valve after performing the pressure test and before resuming normal instrument operation.

Performing the pressure test 1-11

To repeat the pressure test:

1. Repeat step 1 to step 13 from “Performing the pressure test” on page 1-9.

2. If the source fails the pressure test, do not reconnect or start the solvent delivery system; contact Waters.See also: “Contacting Waters” on page 1-13.

If the source passes the pressure test, prepare the instrument for normal operation. See the next section.

Returning the instrument to normal operation

Required materialsChemical-resistant, powder-free gloves

To return the instrument to normal operation:

1. Disconnect the tubing leading to the laboratory exhaust port from the source exhaust valve’s outlet connection.

2. Disconnect the short length of tubing fitted to the source exhaust valve from the nitrogen exhaust waste bottle’s outlet connection.

3. Connect the free end of the tubing leading to the laboratory exhaust port to the nitrogen exhaust waste bottle’s outlet connection (so that the waste bottle’s outlet connects directly to the laboratory exhaust port).

Warning: If the source fails the pressure test, the integrity of its waste system can be impaired; do not reconnect or start the solvent delivery system; contact Waters.

Caution: You must remove the source exhaust valve after performing the source pressure test and before resuming normal instrument operation.

Warning: The solvent delivery and nitrogen exhaust waste line connections can be contaminated with biohazardous and toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.


4. Disconnect the pressure test meter tube from the tee-piece.

5. Fit the blanking plug to the tee-piece; ensure that it is pressed in firmly.

6. Connect the solvent delivery line to the probe.

7. On the MassLynx Tune window, click to start the gas flow.

8. Click Press for Operate, and confirm that the instrument status indicator shows green.

9. Start the solvent delivery system.

Contacting Waters

If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800 252-4752). If you are located anywhere else, phone Waters’ corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. Our Web site includes phone numbers and e-mail addresses for Waters locations worldwide. To view them, go to http://www.waters.com and click About Waters > Worldwide Offices.However you contact Waters, be prepared to provide your mass spectrometer’s serial number.

Contacting Waters 1-13


A Safety Advisories

Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them. This chapter presents all the safety symbols and statements that apply to the entire line of Waters products.

Contents

Topic PageWarning symbols A-2Caution symbol A-5Warnings that apply to all Waters instruments A-5Electrical and handling symbols A-12

A-1

Warning symbols

Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.

Task-specific hazard warningsThe following warning symbols alert you to risks that can arise when you operate or maintain an instrument or instrument component. Such risks include burn injuries, electric shocks, ultraviolet radiation exposures, and others. When the following symbols appear in a manual’s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire)

Warning: (Risk of needle puncture.)

Warning: (Risk of injury caused by moving machinery.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

Warning: (Risk of exposure to a toxic substance.)

A-2 Safety Advisories

Warnings that apply to this instrumentThe following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts.

Burst warning

This warning applies to Waters instruments fitted with nonmetallic tubing.

Mass spectrometer flammable solvents warning

This warning applies to instruments operated with flammable solvents.

Warning: (Risk of personal exposure to laser radiation.)

Warning: (Risk of exposure to biological agents that can pose a serious health threat.)

Warning: Pressurized nonmetallic, or polymer, tubing can burst. Observe these precautions when working around such tubing:• Wear eye protection.• Extinguish all nearby flames.• Do not use tubing that is, or has been, stressed or kinked.• Do not expose nonmetallic tubing to incompatible compounds like

tetrahydrofuran (THF) and nitric or sulfuric acids.• Be aware that some compounds, like methylene chloride and

dimethyl sulfoxide, can cause nonmetallic tubing to swell, which significantly reduces the pressure at which the tubing can rupture.

Warning: Where significant quantities of flammable solvents are involved, a continuous flow of nitrogen into the ion source is required to prevent possible ignition in that enclosed space. Ensure that the nitrogen supply pressure never falls below 400 kPa (4 bar, 58 psi) during an analysis in which flammable solvents are used. Also ensure a gas-fail connection is connected to the HPLC system so that the LC solvent flow stops if the nitrogen supply fails.

Warning symbols A-3

Mass spectrometer shock hazard

This warning applies to all Waters mass spectrometers.

This warning applies to certain instruments when they are in Operate mode.

Biohazard warning

This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.

Warning: To avoid electric shock, do not remove the mass spectrometer’s protective panels. The components they cover are not user-serviceable.

Warning: High voltages can be present at certain external surfaces of the mass spectrometer when the instrument is in Operate mode. To avoid non-lethal electric shock, make sure the instrument is in Standby mode before touching areas marked with this high voltage warning symbol.

Warning: Waters's instruments and software can be used to analyze or process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials. To avoid infection with these agents, assume that all biological fluids are infectious, observe good laboratory practices and, consult your organization’s biohazard safety representative regarding their proper use and handling. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL).


Chemical hazard warning

This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material.

Caution symbol

The caution symbol signifies that an instrument’s use or misuse can damage the instrument or compromise a sample’s integrity. The following symbol and its associated statement are typical of the kind that alert you to the risk of damaging the instrument or sample.

Warnings that apply to all Waters instruments

When operating this device, follow standard quality control procedures and the the equipment guidelines in this section.

Warning: Waters instruments can be used to analyze or process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practices (GLP), and consult your organization’s safety representative regarding proper use and handling. Guidelines are provided in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals.

Caution: To avoid damage, do not use abrasives or solvents to clean the instrument’s case.

Caution symbol A-5

Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen.

Avvertenza: eventuali modifiche o alterazioni apportate a questa unità e non espressamente approvate da un ente responsabile per la conformità annulleranno l’autorità dell’utente ad operare l’apparecchiatura.

Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo.


Warning: Use caution when working with any polymer tubing under pressure:• Always wear eye protection when near pressurized polymer tubing.• Extinguish all nearby flames.• Do not use tubing that has been severely stressed or kinked.• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated

nitric or sulfuric acids.• Be aware that methylene chloride and dimethyl sulfoxide cause

nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing.

Attention: Manipulez les tubes en polymère sous pression avec precaution:• Portez systématiquement des lunettes de protection lorsque vous vous

trouvez à proximité de tubes en polymère pressurisés.• Eteignez toute flamme se trouvant à proximité de l’instrument.• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane

(THF) ou de l'acide sulfurique ou nitrique concentré.• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le

gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture.

Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht:• In der Nähe von unter Druck stehenden Polymerschläuchen stets

Schutzbrille tragen.• Alle offenen Flammen in der Nähe löschen.• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht

sind.• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder

konzentrierte Salpeter- oder Schwefelsäure verwenden.• Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische

Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert.

Warnings that apply to all Waters instruments A-7

Attenzione: prestare attenzione durante l’utilizzo dei tubi di polimero pressurizzati:• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero

pressurizzati.• Estinguere ogni fonte di ignizione circostante.• Non utilizzare tubi soggetti che hanno subito sollecitazioni eccessive o son

stati incurvati.• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido

solforico o nitrico concentrato.• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano

rigonfiamento nei tubi non metallici, riducendo notevolmente la resistenza alla rottura dei tubi stessi.

Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión:• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de

tubos de polímero sometidos a presión.• Si hubiera alguna llama las proximidades.• No se debe trabajar con tubos que se hayan doblado o sometido a altas

presiones.• Es necesario utilizar tubos de metal cuando se trabaje con

tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados.• Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de

dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos.



Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment can be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: l’utente deve essere al corrente del fatto che, se l’apparecchiatura viene usta in un modo specificato dal produttore, la protezione fornita dall’apparecchiatura potrà essere invalidata.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes.


Warning: To protect against fire hazard, replace fuses with those of the same type and rating.

Attention: Remplacez toujours les fusibles par d’autres du même type et de la même puissance afin d’éviter tout risque d’incendie.

Vorsicht: Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen.

Attenzione: per una buona protezione contro i rischi di incendio, sostituire i fusibili con altri dello stesso tipo e amperaggio.

Advertencia: sustituya los fusibles por otros del mismo tipo y características para evitar el riesgo de incendio.


Electrical and handling symbols

Electrical symbolsThese can appear in instrument user manuals and on the instrument’s front or rear panels.

Warning: To avoid possible electrical shock, disconnect the power cord before servicing the instrument.

Attention: Afin d’éviter toute possibilité de commotion électrique, débranchez le cordon d’alimentation de la prise avant d’effectuer la maintenance de l’instrument.

Vorsicht: Zur Vermeidung von Stromschlägen sollte das Gerät vor der Wartung vom Netz getrennt werden.

Attenzione: per evitare il rischio di scossa elettrica, scollegare il cavo di alimentazione prima di svolgere la manutenzione dello strumento.

Precaución: para evitar descargas eléctricas, desenchufe el cable de alimentación del instrumento antes de realizar cualquier reparación.

Electrical power on


Handling symbolsThese handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments.

Electrical power off

Standby

Direct current

Alternating current

Protective conductor terminal

Frame, or chassis, terminal

Fuse

Recycle symbol: Do not dispose in municipal waste.

Keep upright!

Keep dry!

Electrical and handling symbols A-13

Fragile!

Use no hooks!


Index

Numerics4-mm tee-piece 1-2

fitting 1-3

Bbiohazard warning A-4blanking plug 1-2, 1-7, 1-13burst warning A-3

Ccaution symbol A-5chemical hazard warning A-5contacting Waters 1-13criteria for correct source operation

1-11

Ddesolvation gas line 1-2

Eelectrical symbols A-12equipment guidelines A-5

Ffitting

4-mm tee-piece 1-3source exhaust valve 1-5

flammable solvents A-3

Hhandling symbols A-13

Iinstrument

preparing for pressure test 1-7returning to normal operation 1-12

Mmass spectrometer shock hazard A-4

meter, pressure test 1-2

Nnitrogen exhaust waste bottle 1-6

Ppressure test

performing 1-9preparing instrument for 1-7

pressure test meter 1-2

Ssafety advisories A-1source exhaust valve 1-2, 1-3

closed 1-10fitting 1-5open 1-9

source pressure test unitfitting 1-2when to use 1-2

symbolscaution A-5electrical A-12handling A-13warning A-2

Vvalve, source exhaust 1-3

Wwarning symbols A-2, A-5waste bottle, nitrogen exhaust 1-6Waters, contacting 1-13

Index-1

Index-2

Documents

Waters Source Pressure Test Unit Operator’s Guide · Contact Waters ® with enhancement requests or technical quest ions regarding the use ... Fitting the source pressure test unit