Vol. 268, No. 15 The Journal of May 25,1993

Biological Chemistry Copyright 0 1993 by the American Society for Biochemistry and Molecular Biology, Inc.

428 East Preston St., Baltimore, MD 21202 U.S.A.

10701

10705

10709

10713

10717

10721

10728

10739

10746

10754

10760

10766

10773

10780

10789

CONTENTS*

MINIREVIEW 10796 Cloning and characterization of complementary DNA encoding the eukaryotic initiation factor 2-associated 67-kDa protein ( ~ 6 7 ) . Shiyong Wu, Swati Gupta, Nabendu Chatterjee, Ronald E. Hileman, Terry G. Kinzy, Nancy D. Denslow, William C. Merrick, Debopam Chakrabarti, John C. Osterman. and Naba K. Gupta

Oligonucleotide x-ray structures in the study of confor- mation and interactions of nucleic acids. Olga Kennard and Stephen A. Salisbury

COMMUNICATIONS

ture of an antibody-protein antigen complex. Lata Pra- Evaluation of mutagenesis for epitope mapping. Struc-

sad, Sadhann Sharma, Margaret Vandonselaor, J . Wilson Quail, Jeremy S. Lee, E. .Bruce Waygood, Keith S. Wilson, Zbigniew Dauter, and Louls T. J . Delbaere

A novel functional target for tumor-promoting phorbol esters and lysophosphatidic acid. The p2lrac-GTPase activating protein n-chimaerin. Sohail Ahmed, Joel +e, Robert Kozma, Anthony Best, Clcnton Monfries, and Louis Lcm

The active site substrate specificity of the CAMP-

Mendelow, Jaya Srinivasan, Tae Ryong Lee, Scott Pluskey, dependent protein kinase. Young-Guen Kwon, Marianne

Allen Salerno, and David S. Lawrence

A new isoform of 11b-hydroxysteroid dehydrogenase

Naray-Fejes-Toth in aldosterone target cells. Erzsebet Rusvai and Aniko

ARTICLES GimS and G , selectively associate with the cloned so- matostatin receptor subtype SSTR2. S F a n F. Law, Ka- zuki Yasuda, Graeme I. Bell, and Terry Relscne

bovine plasma retinol-binding protein. Giuseppe Zanotti, Crystal structure of liganded and unliganded forms of

Rodolfo Berni, and Hugo L. Monaco

The two allele sequences of a common polymorphism in the promoter of the plasminogen activator inhibitor-1 (PAL-1) gene respond differently to interleukin-1 in

sten, Fiona Green, Steve Humphries, and Adriano M. Henney HepG2 cells. Sally J . Dawson, Bjorn Wiman, Anders Ham-

Alanine dehydrogenase from soybean nodule bacter- oids. Kinetic mechanism and pH studies. Mark T. Smith and David W. Emerich

Phospholipase C-y, associates with viral and cellular src kinases. Osamu Nakanishi, Futoshi Shibasaki, Michihiro Hidaka, Yoshimi Homma, and Tadaomi Takennwa

bly of the active heterodimer. Evidence for folding Refolding of luciferase subunits from urea and assem-

intermediates that precede and follow the dimerization step on the pathway to the active form of the enzyme. Miriam M. Ziegler, Michel E. Goldberg, A k i n F. Chaffotte, and Thomas 0. Baldwin

Contribution of folding steps involving the individual subunits of bacterial luciferase to the assembly of the active heterodimeric enzyme. Thomas 0. Baldwin, Miriam M. Ziegler, Alain F. Chaffotte, and Michel E. Goldberg

heterodimeric intermediate in equilibrium with the na- Folding of bacterial luciferase involves a non-native

tive enzyme and the unfolded subunits. A . Clay Clark, James F. Sinclair, and Thomas 0. Baldwin

Divergent regulation of phosphatidylinositol 3-kinase PS5a and PS5B isoforms upon T cell activation. Karin Recf, Ivan Gout, Michuel D. Waterfield, and Doreen A. Cantrell

Stimulation of aged human lung fibroblasts by extra- cellular ATP via suppression of arachidonate metabo- lism. Ning-nu Hung , Ding-ji Wang, and Leon A. Heppel

10802 Functional size analysis of F-ATPase from Escherichia coli by radiation inactivation. Jing Tyan Ma, JIU Jiu Wu, Chi Meng Tzeng, and Rong Long Pan

10808 Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells. Trrstram 0. Bahnson, Stephen J . Pandol, and Vincent E. Dionne

10813

10820

10826

10836

10842

10851

10863

10870

10876

10881

10888

10895

10900

10906

homologue to Escherichia coli ribosomal protein 512. A novel cloning strategy reveals the gene for the yeast

Lefa E. Alksne and Jonathan R. Warner

Effects of brefeldin A and accessory proteins on am- ciation of ADP-ribosylation factors 1, 3, and 5 with

Moss, and Martha Vaughan Golgi. Su-Chen Tsai, Ronald Adamik, Randy S. Haun, Joel

Differential usage of the carboxyl-terminal region among aldolase isozymes. Luc Berthiaume, Dean R . Tolan, and Jurgen Sygusch

Selective induction by phenobarbital of the electrogenic transport of glutathione and organic anions in rat liver canalicular membrane vesicles. Jose Carlos Fern&- dez-Checa, Murad Ookhtens, and Neil Kaplowitz

Kinetic studies on benzyl alcohol dehydrogenase en-

containing long chain alcohol dehydrogenase family. coded by TOL plasmid pWW0. A member of the zinc-

Jeffrey P. Shaw, Monique Rekik, Frawoise Schwager, and Shigeaki Harayama

Characterization of RNA and DNA synthesis in Esch- erichia coli strains devoid of ppGpp. V. James Hernandez and Hans Bremer

Purification of an autocrine growth factor homologous with mouse epithelin precursor from a highly tumori- genic cell line. Jian Zhou, Guang Gao, John W. Crabb, and Ginette Serrero

Rat liver imidase. Yuh-Shyong Yang, Sengoda Ramaswamy, and William B. Jakoby

Calcium ionophore-induced transient down-regulation of c-myb mRNA levels in Friend erythroleukemia cells. Andrcis Schaefer, Ulrcch Stocker, and Hans Marquardt

heparan sulfate proteoglycan, and zinc. Dieter Reinhardt, Mapping of nidogen binding sites for collagen type IV,

Karlheinz Mann, Roswitha Nischt, Jay W . Fox, Mon-Li Chu, Thomas Krieg, and Rupert Timpl

Interaction of phosphofructokinase with tubulin and microtubules. Quantitative evaluation of the mutual

Judit Ouadi effects. Attila Lehotzky, Marianna Telegdi, Karoly Liliom, and

Identification of a novel subunit of the Type I inter- feron receptor localized to human chromosome 21. Oscar R. Colamonici and Paul Domanski

Structure-function studies of the human thyrotropin receptor. Inhibition of binding of labeled thyrotropin (TSH) by synthetic human TSH receptor peptides. John C. Morris, Elizabeth R. Bergert, and Daniel J . McCormick

Vitamin E recycling in human erythrocyte membranes. Anastasia Constantinescu, Derick Hun, and Lester Packer

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full Instructions to Authors will be found in (1992) J. Bwl. Chern. 268, 745-753, and reprints may be obtained from the editorial office.

11

111 ...

10914

10920

10927

10932

10938

10946

10953

10961

10969

10977

10983

10990

1

1:

0997

1002

1008

1018

1028

1041

Interaction of GTPase activating proteins (GAPS) with p21- measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on ~ 2 1 ~ " . Geoffrey G. Brownbridge, Peter N. Lowe, Keith J. M. Moore, Richard H. Skinner, and Martin R. Webb

Addition of a heterologous transmembrane domain to Structure-function analysis of the Bcl-2 oncoprotein.

portions of the Bcl-28 protein restores function as a regulator of cell survival. Shigeki Tanakn, Koichi Saito, and John C. Reed

protein kinase inhibitor of CAMP-dependent protein Evidence for two additional isoforms of the endogenous

kinase in mouse. Marc0 A. Scarpetta and Michael D. Uhler

A novel protein domain required for apoptosis. Muta- tional analysis of human Fas antigen. Naoto Itoh and Shigekazu Nagata

Mannosamine inhibits the synthesis of putative glyco- inositol phospholipid anchor precursors in mammalian cells without incorporating into an accumulated inter- mediate. Daniel Sevlever and Terrone L. Rosenberry

Acetyl-coA carboxylase from yeast is an essential en- zyme and is regulated by factors that control phospho- lipid metabolism. Meinhard Hafilacher, Andreas S. Iuessa, Fritz Paltauf, and Sepp D. Kohlwein

Cal+ binding to sarcoplasmic reticulum ATPase revis- ited. I. Mechanism of affinity and cooperativity modu- lation by H+ and M8+. Vincent Forge, Elisabeth Mintz, and Florent Guilluin

Cap+ binding to sarcoplasmic reticulum ATPase revis- ited. 11. Equilibrium and kinetic evidence for a two- route mechanism. Vincent Forge, Elisabeth Mintz, and Flo- rent Guilluin

Binding and regulatory properties of expressed func- tional domains of chicken gizzard smooth muscle cal- desmon. Charles S. Redwood and Steuen B. Marston

Isolation and characterization of the human sprl gene and its regulation of expression by phorbol ester and cyclic AMP. Gang An, Johannes Tesfaigzi, Yue-Jen Chuu, and Reen W u

Transient transfection studies of secretion in bovine chromaffin cells and PC12 cells. Generation of kainate- sensitive chromaffin cells. Peter F. Wick, Ruth A. Senter, Leslie A. Parsels, Michael D. Uhler, and Ronald W. Holz

Regulation of linkages between the erythrocyte mem- brane and its skeleton by 2,3-diphosphoglycerate. Ryu- ichi Moriyama, Christian R. Lombardo, Ryan F. Workman, and Philip S. Low

Ca'+-dependent kinase and phosphatase control inositol

tion by agonist stimulation. Bin-Xian Zhang, Hong Z h , 1,4,5-trisphosphate-mediated Cap+ release. Modifica-

and Shmuel Muallem

Purification and characterization of farnesyl diphos- phate/geranylgeranyl diphosphate synthase. A ther- mostable bifunctional enzyme from Methanobacterium thermoautotrophicum. Anjun Chen and C. Dale Poulter

Functional interactions between SV40 T antigen and other replication proteins at the replication fork. Yota Muraknmi and Jerard Hurwitz

DNA polymerase a stimulates the ATP-dependent bind- ing of simian virus tumor T antigen to the SV40 origin of replication. Yota Murakami and Jerard Hurwitz

Mutational analysis of the human DNA polymerase a. The most conserved region in a-like DNA polymerases is involved in metal-specific catalysis. William C. C o p - Land and Teresa S.-F. Wang

Fidelity studies of the human DNA polymerase a. The most conserved region among a-like DNA polymerases is responsible for metal-induced infidelity in DNA syn- thesis. William C. Copeland, Naomi K. Lam, and Teresa S.-F. wang

11050 A human cDNA corresponding to a gene overexpressed during cell proliferation encodes a product sharing ho-

Marie-Th2rese Prosperi, Didier Ferbus, Isabelle Karczinski, mology with amoebic and bacterial proteins.

and Gerard Goubin

11057 Botulinum toxin inhibits arachidonic acid release as- sociated with acetylcholine release from PC12 cells. Prabhnti Ray, Jonathan D. Berman, Wilbert Middleton, and James Brendle

11065 Regulation by membrane potential of phosphatidyl-

Aidan G. M. Dauison, and Monica L. Prugue inositol hydrolysis in pancreatic islets. Trevor J . Biden,

11073 Characterization of glycoprotein I1 from bovine adre- nal medullary chromaffin granules. Identification of

parts of the lysosomal-associated membrane glycopro- components representing the secretory vesicle counter-

teins (lamp-1 and lamp-2). A. David Hieber and Dauid L. Christie

11079 Sterol 27-hydroxylase in bile acid biosynthesis. Mech- anism of oxidation of 5&cholestane-3a,7a,l2a,27-te- trol into 3a,7a,l2a-trihydroxy-5~-cholestanoic acid. Ingrid Holmberg-Betsholtz, Erik Lund, Ingemar Bjorkhem, and Kjell Wikvall

11086

11090

11097

11105

11113

11119

11125

11134

Mutants of the Bacillus subtilis multidrug transporter Bmr with altered sensitivity to the antihypertensive

Alexander A. Neyfakh, and Shimon Schuldiner alkaloid reserpine. Maqbool Ahmed, Carina M. Borsch,

Circular dichroism studies of diethyl pyrocarbonate-

Li, Dexter S. Moore, and Robert C. Rosenberg modified histidine in hen egg white lysozyme. Chingwen

Topography of glycosylation and UDP-xylose produc- tion. Ann E. Kearns, Barbara M. Vertel, and Nancy B. Schwartz

Xylosylation is an endoplasmic reticulum to Golgi

E. Kearns, and Nancy B. Schwartz event. Barbara M. Vertel, Linda M. Walters, Nina Flay, Ann

Saccharomyces cerevisiae CDC25(1028-1589) is a guanine nucleotide releasing factor for mammalian Ras

Marie-Christine Chevallier-Multon, Fabien Schweighoffer, proteins and is oncogenic in NIH3T3 cells.

Isabelle Barlut, Nicole Baudouy, Isabelle Fath, Marc Duchesne, and Bruno Tocqui

phosphorylation in regulated secretion by the pan- A role for cholecystokinin-stimulated protein tyrosine

creatic acinar cell. Manfred P. Lutz, Shari L. Sutor, Robert T. Abraham, and Luurence J. Miller

Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) represses transcription from the promoter of the gene for ornithine transcarbamylase in a manner antagonistic to hepatocyte nuclear factor-4 (HNF-4). Akira Kimura, Atsushi Nishiyori, Takashi Mura- kami, Toshlro Tsukamoto, Shingo Hata, Takashi Osumi, Ry- oichi Okamura, Masataka Mori, and Masaki Takiguchi

HER2/Neu receptors by tyrphostins. Nir Osherou, Aviv Selective inhibition of the epidermal growth factor and

Gazit, Chaim Gilon, and Alexander Leuitzki

143 Preparation and characterization of a set of deoxyoli- gonucleotide 49-mers containing site-specific cis- syn, trans-syn-I, (6-4), and Dewar photoproducts of

John-Stephen Taylor thymidylyl(3' 4 5')-thymidine. Colin A. Smith and

152 N-Linked glycosylation of the ligand-binding domain of the human urokinase receptor contributes to the affinity for its ligand. Lisbeth Birk Mdler, Juri Polliinen, Ebbe RBnne, Nina Pedersen, and Francesco B h i

11160 Cis-active elements controlling lung cell-specific expression of human pulmonary surfactant protein B gene. Robert J . Bohinski, Jacquelyn A. Huffman, Jeffrey A. Whitsett, and Dauid L. Luttier

iv

11167

11172

11176

11188

11193

11199

11208

11217

11222

11230

11239

11247

11256

11265

11272

11278

11283

11290

Insulin stimulates association of insulin receptor sub- strate-1 with the protein abundant Src homology/

Koozi Matuoka, Hiroyuki Tammoto, Kohjiro Ueki, Yasushi growth factor receptor-bound protein 2. Kazuyuki Tobe,

Kaburagi, Shohji Ami, Tetsuya Noguchi, Michiyuki Mat- suda, Shinya Tanaka, Seisuke Hattori, Yasuhisa Fukui, Yasuo Akanumn, Yoshio Yazaki, Tadaomi Takenawa, and Taknshi Kadowaki

Comparative analysis of two alternative first exons reported for the mouse osteopontin gene. Elke I . Behrend, Ann F. Chambers, Sylvia M. Wilson, and David T. Denhardt

phorylation of kinesin light chains modulate kinesin Calmodulin binding to and CAMP-dependent phos-

ATPase activity. Heinrich J . G. Matthies, Richard J . Miller, and H. Clive Palfrey

Characterization of the receptor-binding domain of tet- anus toxin. Jane L. Halpern and Anne Loftus

Purification and characterization of an autophosphor- ylation-activated protein serine threonine kinase that phosphorylates and inactivates protein phosphatase 2A. Hong Guo, Shrikanth A. G. Reddy, and Zahi Damuni

A novel RNA polymerase 111 transcription factor frac-

gio Dieci, Laura Duimio, Federico Coda-Zabetta, Karen U. tion that is not required for template commitment. Gior-

Sprague, and Simone Ottonella

Functional erythropoietin receptor of the cells with neural characteristics. Comparison with receptor prop- erties of erythroid cells. Seiji Masuda, Masaya Nqao, Kyoya Takahata, Yoshihiro Konishi, Ferenc Gallyas, Jr., Tak- eshi Tabira, and Ryuzo Sasaki

Human glyoxalase 1. cDNA cloning, expression, and sequence similarity to glyoxalase I from Pseudomonas putida. Nam-Soon Kim, Yuri Umezawa, Satoshi Ohmura, and Seishi Kato

A mutational analysis of the polypyrimidine tract of introns. Effects of sequence differences in pyrimidine tracts on splicing. Robert F. Roscigno, Michael Weiner, and Mariano A. Garcia-Blanco

Tocotrienols regulate cholesterol production in mam- malian cells by post-transcriptional suppression of 3-

A. Parker, Bradley C. Pearce, Ronald W. Clark, David A. hydroxy-3-methylglutaryl-coenzyme A reductase. Rex

Gordon, and J. J. Kim Wright

trihydroxy nor-bile acids as new major biotransfor- 6@-Hydroxylation by the liver. Identification of 3,6,7-

dents. Claua‘io D. Schteingart, t e e R. Hctgey, Kenneth D. R. mation products of 3,7-dihydroxy nor-bile acids in ro-

Setchell, and Alan F. Hofmann

Phosphorylation weakens DNA binding by peptides containing multiple “SPKK” sequences. George R. Green, Hyuck-Jin Lee, and Dominic L. Poccia

Insulin stimulates serine and tyrosine phosphorylation in the juxtamembrane region of the insulin receptor.

A. Wilden, Xino Jian Sun, C. Ronald Kahn, and Morris F. Edward P. Feener, Jonathan M. Backer, George L. King, Peter

White

Fate of DNA targeted to the liver by asialoglycoprotein

sistence in cytoplasmic vesicles after partial hepatec- receptor-mediated endocytosis in vivo. Prolonged per-

tomy. Namita Roy Chowdhury, Catherine H. Wu, George Y.

Roy Chowdhury Wu, Purna C. Yemeni, Vasudeva R. Bommineni, and Jayanta

Antibodies to the extracellular receptor domain restore

the mutant insulin receptor valine 382. Christine Lebrun, the hormone-insensitive kinase and conformation of

Vkronique Baron, Perla Kaliman, Nadine Gautier, Jacqueline Dolais-Kitabgi, S i m o n Taylor, Domenico Accili, and Emman- uel Van Obberghen

Symmetrical disulfide bonds are not necessary for as- sembly and secretion of human fibrinongen. Jian-Zhong Zhang, Bohdan Kudryk, and Colvin M. Redman

Janet S. van Adelsberg, John C. Edwards, and Qais Al-Awqati The apical C1/HCOs exchanger of @ intercalated cells.

Arachidonic acid-selective cytosolic phospholipase A2 is crucial in the cytotoxic action of tumor necrosis

Sayumi Shibamoto, Takamitsu Hori, Naoto Oku, Fumiaki Ito, factor. Makio Hayakawa, Nobuhiro Ishida, Kenji Takeuchi,

and Masafumt Tsujimoto

11296

11304

11312

11321

11326

11335

11340

11348

11356

11364

11372

11380

11389

11394

11401

11409

11417

11426

Cloning and characterization of a putative Caa+m+ an- tiporter gene from Emherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene. D. Mack h e y , Arthur A. Guf- fanti, Jennifer Zemsky, Elhanan Pinner, Rachel Karpel, Etana Padan, Shtmon Schuldtner, and Terry A. Krulwich

Protein kinase A (PKA) still activates CFTR chloride

phosphorylation sites. Xiu-Bao Chang, Joseph A. Tabchar- channel after mutagenesis of all 10 PKA consensus

ani, Yue-Xian Hou, Timothy J . Jensen, Norbert Kartner, Noa Alan, John W. Hanrahan, and John R. Riordan

cludes two protein-binding sites in the promoter region. Structure of the canine pancreatic colipase gene in-

Shin-lchi Fukuoka, Dong-Er Zhang, Yukio Taniguchi, and George A . Scheele

Protein kinase C activates chloride conductance in C127 cells stably expressing the cystic fibrosis gene. Maria Cristina Dechecchi, Anna Tamanini, Giorgio Berton, and Giulio Cabrini

Molecular analysis of the essential gene for adenylate

pombe. Manfred Konrad kinase from the fission yeast Schizosaccharomyces

FK-606-binding protein: three-dimensional structure of the complex with the antagonist L-685,818. Joseph

Chan, Alice I. Marcy, Greg Wiederrecht, Jeffrey D. Hermes, W. Becker, Jennifer Rotonda, Brian M. McKeeuer, H. Karen

and James P. Springer

basolateral membrane domain of Madin-Darby canine The aZA-adrenergic receptor is targeted directly to the

kidney cells independent of coupling to pertussis toxin- sensitive GTP-binding proteins. Jeffrey R. Keefer and Lee E. Limbird

Three-dimensional structures of the periplasmic lysine/

ligand. Byung-Ha Oh, Jayuardhan Pundit, Chul-Hee Kang, arginine/ornithine-binding protein with and without a

Kishiko Nikaido, Sabiha Gokcen, Giovanna Ferro-Luzzi Ames, and Sung-Hou Kim

Sequence and functional characterization of a third

pressed in pancreatic islets, kidney, gastrointestinal inositol trisphosphate receptor subtype, IP3R-3, ex-

tract, and other tissues. Olivier Blondel, Jun Takeda, Heil- wig Janssen, Susumu Seino, and Graeme I. Bell

Mapping regions of yeast transcription factor IIIA re- quired for DNA binding, interaction with transcription

and Jacqueline Segall factor IIIC, and transcription activity. Catherine A. Milne

A closely linked complex of mouse mast cell-specific chymase genes on chromosome 14. Michael F. Gurish, Joseph H. Nadeau, Kenneth R. Johnson, H. Patrick McNeil, Kathleen M. Grattan, K. Frank Austen, and Richard L. Stevens

Oxidoreductive regulation of nuclear factor K B. In- volvement of a cellular reducing catalyst thioredoxin. Takumn Hayashi, Yoshw Ueno, and Taknshi Okamoto

High mobility group proteins 14 and 17 can space nu- cleosomes in vitro. David John Tremethick and Horace R. Drew

P-selectin is acylated with palmitic acid and stearic

Fujimoto, Eric Stroud, Ralph E. Whatley, Stephen M. Prescott, acid at cysteine 766 through a thioester linkage. Tetsuro

Laszlo Muszbek, Michael Laposata, and Rodger P. McEver

Inside-out integrin signaling in macrophages. Analysis of the role of the a6AB1 and a6BBl integrin variants in laminin adhesion by cDNA expression in an a6 in- tegrin-deficient macrophage cell line. Leslie M. Show, Margaret M. Lotz, and Arthur M. Mercurio

Translational repression of endogenous thymidine ki- nase mRNA in differentiating and arresting mouse cells. Martin KrGfler, Christian Waltner, Erhard Wintersber- ger, and Ernst W. Miillner

Major photoaffinity drug labeling sites for iodoaryl azidoprazosin in P-glycoprotein are within, or imme- diately C-terminal to, transmembrane domains 6 and 12. Lee M. Greenberger

Regulatory sequences on the human villin gene trigger the expression of a reporter gene in a differentiating

quilt%-Merino, Daniel Louuard, and Eric Pringault HT29 intestinal cell line. Sylvie Robine, Christian Sahu-

V

11435 Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEKl and MEK2. C h - F e n g Zheng and Kun-Liang G u m

11440 Organization and promoter activity of the mouse syn-

E. Neumann, Robert Kokenyesi, and Merton Bernfield decan-1 gene. Michael T. Hinkes, Olga A. Goldberger, Paul

11449 Aldehyde dehydrogenase-derived R-crystallins of squid

Zinoukua, Stanlsluu I. Tomarev, and Joram Piatigorsky and octopus. Specialization for lens expression. R i m D.

11456 Mouse procathepsin L lacking a functional glycosyla- tion site is properly folded, stable, and secreted by NIH 3T3 cells. Susan E. Kane

11463 Se uences in the 3’-untranslated region of the human c e l h r glutathione peroxid- gene are necessary and sufficient for selenocysteine mcorporatlon at the UGA codon. Qichang Shen, Fong-Fong Chu, and Peter E. Newburger

11470 The terminal quinol oxidasp of Bacillus subtilis have different energy conservation properties. Mark0 Lau- roeus and M&ten Wzkstrom

ADDITIONS AND CORRECTIONS 11474 Overexpression in Escherichia coli and affinity puri-

fication of chick kidney ferredoxin. Vol. 268 (1993) 6069-5076. Chilung Tang and Helen L. Henry

11474 Cuticle surface proteins of wild t e and mutant Cae- norhabditis elegans. Vol. 268 ( 1 9 8 ) 6600-6609. Mark L. Blarter

xi Cumulative author index. Numbers 1 through 16.

GENERAL INFORMATION

your subscription agent or contact: THE JOURNAL OF BIOLOGICAL CHEMISTRY. This publication is available on CD-ROM. Inquire about availability through

428 EAST PRESTON STREET, BALTIMORE MD 21202, ,U.S.A. Minireviews, are reprinted rn January of the succeeding year in a Minireview Cornpendrum.

ASBMB Office, 9650 Rockville Pike, Bethesda, MD 20814, U.S.A. Compendia for 1988 through 1992 are available through the ASBMB office:

Submit all manuscripts in triplicate to Editor, The J o u r n a l of Biological Chemistry 9650 Rockville Pike Bethesda, MD 20814, U.S.A.

Accepted manuscripts will be published with the implicit understanding that the authork) will pay a charge per page. Current page charges may be obtained by contacting the JBC Office. Under exceptional circumstances, when no source of grant or other support exists, the author(s) may apply, at the t ime of submission, for a grant-in-aid to Chairman, Publications Committee, Ameri- can Society for Biochemistry and Molecular Biology, Inc., 9650 Rockville Pike, Bethesda, MD 20814. All such applications must be countersigned by an appropriate institutional official stating that no funds are available for page charges.

Queries on matters of general editorial policy, requests for reprints of the “Instructions to Authors,” or of the “Editorial Policy and Practices,” or for permission to reproduce any part of a previously published article should be directed to the Journal Editorial Office in Bethesda, telephone 301-530-7150.

The Journal of Biological Chemistry publishes papers on a broad range of topics of interest to biochemists. The views expressed are those of the author(s) and not of The Journal of Biological Chemistry or the American Society for Biochemistry and Molecular Biology.

for Biochemistry and Molecular Biology, Inc. Reprographic copying beyond The Journal of Biological Chemistry is copyrighted by the American Society

that permitted by Sections 107 or 108 of the US. Copyright Law is allowed, provided that the $2.50 per-copy fee is paid through the Copyright Clearance Center, 27 Congress Street, Salem, MA 01970. For those organizations that have been granted photocopy license by CCC, a separate system of payment has been arranged. The fee code for users of the Transactional Reporting Service is: 0021-9258/93/$2.50. @ Printed on acid-free paper effective with

subsequent republication requires permission of the copyright owner. Requests Volume 254, Issue No. 1 (1979). Reproduction of any portion of an article for

should be made in writing to the American Society for Biochemistry and

MD 20814, and should include a statement of intended use as well as explicit Molecular Biology, Inc., Attn.: Editorial Office, 9650 Rockville Pike, Bethesda,

specifications of the material to be reproduced.

copies to: THE JOURNAL OF BIOLOGICAL CHEMISTRY, 428 EAST PRESTON Address all correspondence and orders relative to subscriptions and back

STREET, BALTIMORE, MD 21202, U.S.A., telephone 410-528-4255. Subscrip- tions are entered on a calendar year basis only. Requests for termination of

charge. Allow a t least six weeks for address changes. Claims for replacement subscriptions will be honored with proportional refunds less a $10 cancellation

copies must be received within four nionths of the issue date.

Vol. 268, No. 15 The Journal of Biological Chemistry May 25,1993

CONTENTS Arranged by Subject Categories

MINIREVIEW 10946 Acetyl-coA carboxylase from yeast is an essential en- zyme and is regulated by factors that control phospho- lipid metabolism. Meinhard HaBlacher, Andreas S. luessa,

10701 Oligonucleotide x-ray structures in the study of confor- mation and interactions of nucleic acids. Olga Kennard and Stephen A. Salisbury Fritz Paltauf, and Sepp D. Kohlwein

10754

10938

11002

11239

10721

10780

10789

10808

10820

10863

10876

10888

10914

10920

10927

10932

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

Phospholipase C-71 associates with viral and cellular 8rc kinases. Osamu Nakanishi, Futoshi Shibasaki, Michihiro Hidaka, Yoshimi Homma, and Tadaomi Takenawa

Mannosamine inhibits the synthesis of putative glyco- inositol phospholipid anchor precursors in mammalian cells without incorporating into an accumulated inter- mediate. Daniel Seuleuer and Terrane L. Rosenberry

Purification and characterization of farnesyl diphos- phate/geranylgeranyl diphosphate synthase. A ther- mostable bifunctional enzyme from Methanobacterium thermoautotrophicum. Anjun Chen and C. Dale Poulter

trihydroxy nor-bile acids as new major biotransfor- 5B-Hydroxylation by the liver. Identification of 3,5,7-

mation products of 3,7-dihydroxy nor-bile acids in ro- dents. Claudw D. Schteingart, Lee R. Hagey, Kenneth D. R. Setchell, and Alan F. Hofmann

CELL BIOLOGY AND METABOLISM G ~ s and G , selectively associate with the cloned so- matostatin receptor subtype SSTR2. Susan F. Law, Ka- zuki Yasuda, Graeme I. Bell, and Terry Reisine

Divergent regulation of phosphatidylinositol 3-kinase

Reif, Ivan Gout, Michael D. Waterfield, and Doreen A. Cantrell P86a and P858 isoforms upon T cell activation. Karin

cellular ATP via suppression of arachidonate metabo- Stimulation of aged human lung fibroblasts by extra-

lism. Ning-na Huang, Ding-ji Wang, and Leon A. Heppel

Cyclic GMP modulates depletion-activated Ca2+ entry in pancreatic acinar cells. Tristram D. Bahmon, Stephen J . Pandol, and Vincent E. Dianne

Effects of brefeldin A and accessory proteins on asso- ciation of ADP-ribosylation factors 1, 3, and 5 with Golgi. Su-Chen Tsai, Ronald Adamik, Randy S. Haun, Joel Moss, and Martha Vaughan

with mouse epithelin precursor from a highly tumori- Purification of an autocrine growth factor homologous

genic cell line. Jiun Zhou, Guang Gm, John W. Crabb, and Ginette Serrero

Calcium ionophore-induced transient down-regulation of c-myb mRNA levels in Friend erythroleukemia cells. And& Schaefer, Ulrich Stocker, and Hans Marquardt

Interaction of phosphofructokinase with tubulin and microtubules. Quantitative evaluation of the mutual effects. Attila Lehotzky, Marianna Telegdi, Karoly Liliom, and Judit Oucidi

Interaction of GTPase activating proteins (GAPS) with p21'" measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21'". Geoffrey G. Brownbridge, Peter N. Lowe, Keith J . M. Moore, Richard H. Skinner, and Martin R. Webb

Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2B protein restores function as a regulator of cell survival. Shigeki Tanaka, Koichi Saito, and John C. Reed

protein kinase inhibitor of CAMP-dependent protein Evidence for two additional isoforms of the endogenous

kinase in mouse. Marc0 A. Scarpetta and Michael D. Uhler

A novel protein domain required for apoptosis. Muta- tional analysis of human Fas antigen. Nmto Itoh and Shigekazu Nagata

10983

10990

10997

11057

11065

11097

11105

11113

11134

11167

11176

11188

11208

11230

11256

Transient transfection studies of secretion in bovine chromaffin cells and PC12 cells. Generation of kainate- sensitive chromaffin cells. Peter F. Wick, Ruth A. Senter, Leslie A. Parsek, Michael D. Uhler, and Ronald W. Holz

Regulation of linkages between the erythrocyte mem- brane and its skeleton by 2,3-diphosphoglycerate. Ryu- ichi Moriyama, Christian R. Lombardo, Ryan F. Workman, and Philip S. Low

Ca2+-dependent kinase and phosphatase control inositol 1,4,5-trisphosphate-mediated Ca2+ release. Modifica- tion by agonist stimulation. Bin-Xiun Zhang, Hong Z h , and Shmuel Muallem

Botulinum toxin inhibits arachidonic acid release as-

Prabhati Ray, Jonathan D. Berman, Wilbert Middleton, and sociated with acetylcholine release from PC12 cells.

James Brendle

Regulation by membrane potential of phosphatidyl-

Aidan G. M. Dauison, and Monica L. Prugue inositol hydrolysis in pancreatic islets. Trevor J . Biden,

Topography of glycosylation and UDP-xylose produc- tion. Ann E. Kearns, Barbara M. Vertel, and Nancy B. Schwartz

Xylosylation is an endoplasmic reticulum to Golgi event. Barbara M. Vertel, Linda M. Walters, Nina Flay, Ann E. Kearns, and Nancy B. Schwartz

Saccharomyces cereuisiae CDC25(1028-1589) is a guanine nucleotide releasing factor for mammalian Ras

Marie-Christine Cheuallier-Multon, Fabien Schweighoffer, proteins and is oncogenic in NIH3T3 cells.

Isabelle Barlat, Nicole Baudouy, Isabelle Fath, Marc Duchesne, and Bruno Tocqui

HER2/Neu receptors by tyrphostins. Nir Osherou, Auiu Selective inhibition of the epidermal growth factor and

Gazit, Chaim Gilon, and Alexander Leuitzki

Insulin stimulates association of insulin receptor sub- strate-1 with the protein abundant Src homology/ growth factor receptor-bound protein 2. Kazuyuki Tobe, Koozi Matuoka, Hiroyuki Tamemoto, Kohjiro Ueki, Yasushi Kaburagi, Shohji Asai, Tetsuya Noguchi, Michiyuki Mat- suda, Shinya Tanaka, Seisuke Hattori, Yasuhisa Fukui, Yasuo Akanuma, Yoshio Yazaki, Tadmmi Takenawa, and Takashi Kadowaki

Calmodulin binding to and CAMP-dependent phos- phorylation of kinesin light chains modulate kinesin ATPase activity. Heinrich J. G. Matthies, Richard J. Miller, and H. Cliue Palfrey

Characterization of the receptor-binding domain of tet- anus toxin. Jane L. Halpern and Anne Loftus

Functional erythropoietin receptor of the cells with neural characteristics. Comparison with receptor prop- erties of erythroid cells. Seiji Masuda, Masaya Nagm, Kyoya Tahhata, Yoshihiro Konishi, Ferenc Gallyas, Jr., Tak- eshi Tabira, and Ryuzo Sasaki

Tocotrienols regulate cholesterol production in mam-

hydroxy-3-methylglutaryl-coenzyme A reductase. Rex malian cells by post-transcriptional suppression of 3-

A. Parker, Bradley C. Pearce, Ronald W. Clark, David A. Gordon, and J . J . Kim Wright

Insulin stimulates serine and tyrosine phosphorylation in the juxtamembrane region of the insulin receptor. Edward P. Feener, Jonathan M. Backer, George L. King, Peter A. Wilden, Xiao Jian Sun, C. Ronald Kahn, and Morris F. White

vi

vii

11265

11272

.1

.1

.1

283

290

340

11401

11435

-10709

'10713

-10717

10746

10826

10842

10870

10961

11079

11090

11193

Fate of DNA targeted to the liver by asialoglycoprotein receptor-mediated endocytosis in vivo. Prolonged per- sistence in cytoplasmic vesicles after partial hepatec- tomy. Namita Roy Chowdhury, Catherine H. Wu, George Y.

Roy Chowdhury Wu, Purna C. Yerneni, Vasudeva R. Bommineni, and Jayanta

Antibodies to the extracellular receptor domain restore the hormone-insensitive kinase and conformation of the mutant insulin receptor valine 382. Christine Lebrun, Vkronique Baron, Perla Kalimon, Nadjne Gautier, Jacqueline Doluis-Kitabgi, S i m o n Taylor, Domenzco Accrlr, and Emman- uel Van Obberghen

The apical Cl/HCOs exchanger of B intercalated cells. Janet S. van Adekberg, John C. Edwards, and Qais AI-Awqati

Arachidonic acid-selective cytosolic phospholipase AZ is crucial in the cytotoxic action of tumor necrosis factor. Makio Hayakawa, Nobuhim Ishida, Kenji Takeuchi,

and Masafumi Tsujimoto Sayumi Shibamoto, Taknmitsu Hori, Naoto Oku, Fumiaki Ita,

basolateral membrane domain of Madin-Darby canine The aoA-adrenergic receptor is targeted directly to the

kidney cells independent of coupling to pertussis toxin- sensitive GTP-binding proteins. Jeffrey R. Keefer and Lee E. Limbird

Inside-out integrin signaling in macrophages. Analysis of the role of the a6M1 and a6BB1 integrin variants in laminin adhesion by cDNA expression in an (16 in- tegrin-deficient macrophage cell line. Leslie M. Shaw, Margaret M. Lotz, and Arthur M. Mercurm

Cloning and characterization of two distinct human extracellular signal-regulated kinase activator

Kun-Liang Guan kinases, MEKl and MEK2. Chao-Feng Zheng and

ENZYMOLOGY A novel functional target for tumor-promoting phorbol esters and lysophosphatidic acid. The p2lrac-CTPase activating protein n-chimaerin. Sohail Ahmed, Joel Lee, Robert Kozma, Anthony Best, Clinton Monfries, and Louis Lim

The active site substrate specificity of the CAMP-

Mendelow, Jaya Sriniuasan, Tae Ryong Lee, Scott Pluskey, dependent protein kinase. Young-Guen Kwon, Marianne

Allen Salerno, and Dauid S. Lawrence

A new isoform of 1 18-hydroxysteroid dehydrogenase

Naray-Fejes-Toth in aldosterone target cells. Erzsebet Rusvai and Aniko

Alanine dehydrogenase from soybean nodule bacter- oids. Kinetic mechanism and pH studies. Mark T. Smith and David W. Emerich

Differential usage of the carboxyl-terminal region among aldolase isozymes. Luc Berthiaume, Dean R. Tolan, and Jurgen Sygcmh

coded by TOL plasmid pWW0. A member of the zinc- Kinetic studies on benzyl alcohol dehydrogenase en-

Jeffrey P. Show, Monique Rekik, Franpise Schwager, and containing long chain alcohol dehydrogenase family.

Shigeaki Harayama

Rat liver imidase. Yuh-Shyong Yang, Sengoda Ramaswamy, and William B. Jakoby

Cap+ binding to sarcoplasmic reticulum ATPase revis-

route mechanism. Vincent Forge, Elisabeth Mintz, and Flo- ited. 11. Equilibrium and kinetic evidence for a two-

rent Guillain

Sterol 27-hydroxylase in bile acid biosynthesis. Mech- anism of oxidation of S&cholestane-3a,7a,l2a,27-te- trol into 3a,7a,l2a-trihydroxy-5j3-cholestanoic acid. Ingrid Holmberg-Betsholtz, Erik L u d , Ingemar Bjorkhem, and Kjell Wikvoll

Circular dichroism studies of diethyl pyrocarbonate- modified histidine in hen egg white lysozyme. Chingwen Li, Dexter S. Moore, and Robert C. Rosenberg

Purification and characterization of an autophosphor- ylation-activated protein serine threonine kinase that phosphorylates and inactivates protein phosphatase 2A. Hong G w , Shrikanth A. G. Reddy, and Zahi Damuni

11217

10802

10836

10906

10953

11 086

11296

11304

11321

11417

11470

N

10739

10796

10813

1085 1

10977

11008

11018

Human glyoxalase I. cDNA cloning, expression, and sequence similarity to glyoxalase I from Pseudomonas putida. Nam-Soon Kim, Yuri Umezawa, Satoshi Ohmura, and Seishi Kat0

MEMBRANES AND BIOENERGETICS Functional size analysis of F-ATPase from Escherichia coli by radiation inactivation. Jing R a n Ma, Jia Jiu Wu, Chi Meng Tzeng, and Rong Long Pan

Selective induction by phenobarbital of the electrogenic transport of glutathione and organic anions in rat liver canalicular membrane vesicles. Jose Carlos Fernan- dez-Checa, Murad Ookhtens, and Neil Kaplowitz

Vitamin E recycling in human erythrocyte membranes. Anastasia Constantinescu, Derick Han, and Lester Packer

Ca2+ binding to sarcoplasmic reticulum ATPase revis- ited. I. Mechanism of affinity and cooperativity modu- lation by H+ and M8+. Vincent Forge, Elisabeth Mintz, and Florent Guillain

Mutants of the Bacillus subtilis multidrug transporter Bmr with altered sensitivity to the antihypertensive

Alexander A. Neyfakh, and Shimon Schuldiner alkaloid reserpine. Maqbool Ahmed, Carina M. Borsch,

Cloning and characterization of a putative Ca*+/H+ an-

complementation of Na+/H+ antiporter-deficient strains tiporter gene from Escherichia coli upon functional

fanti, Jennifer Zemsky, Elhanan Pinner, Rachel Karpel, Etana by the overexpressed gene. D. Mack hey , Arthur A. Guf-

Padan, Shimon Schuldiner, and Terry A. Kruluxch

Protein kinase A (PKA) still activates CFTR chloride

phosphorylation sites. Xiu-Bao Chang, Joseph A. Tabchar- channel after mutagenesis of all 10 PKA consensus

Alon, John W. Hanrahun, and John R. Rwrdan ani, Yue-Xian Hou, Timothy J. Jensen, Norbert Kartner, Noa

Protein kinase C activates chloride conductance in C127 cells stably expressing the cystic fibrosis gene. Maria Cristina Dechecchi, Anna Tamanini, Gwrgio Berton, and Giulio Cabrini

Major photoaffinity drug labeling sites for iodoaryl azidoprazosin in P-glycoprotein are within, or imme- diately C-terminal to, transmembrane domains 6 and 12. Lee M. Greenberger

The terminal quinol oxidases of Bacillus subtilis have different energy conservation properties. Marko Luu- raeus and Miirten Wikstrom

IUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

The two allele sequences of a common polymorphism in the promoter of the plasminogen activator inhibitor-1

HepG2 cells. Sally J. Dawson, Bjorn Wiman, Anders Ham- (PAI-1) gene respond differently to interleukin-1 in

sten, Fiona Green, Steve Humphries, and Adriano M. Henney

Cloning and characterization of complementary DNA encoding the eukaryotic initiation factor 2-associated 67-kDa protein (pa?). Shiyong Wu, Swati Gupta, Nabendu

Denslow, William C. Merrick, Debopam Chakrabarti, John C. Chatterjee, Ronald E. Hilemun, Terry G. Kinzy, Nancy D.

Osterman, and Naba K. Gupta

A novel cloning strategy reveals the gene for the yeast

Lefa E. Alksne and Jonathan R. Warner homologue to Escherichia coli ribosomal protein 512.

Characterization of RNA and DNA synthesis in Esch- erichia coli strains devoid of ppGpp. V. James Hernandez and Ham Bremer

Isolation and characterization of the human sprl gene and its regulation of expression by phorbol ester and cyclic AMP. Gang An, Johannes Tesfaigzi, Yue-Jen Chuu, and Reen W u

Functional interactions between SV40 T antigen and other replication proteins at the replication fork. Yota Murakami and Jerard Hurwitz

DNA polymerase (1 stimulates the ATP-dependent bind-

of replication. Yota Murakami and Jerard Hurwitz ing of simian virus tumor T antigen to the SV40 origin

Indicates a Communication

viii

11028 Mutational analysis of the human DNA polymerase a. The most conserved region in a-like DNA polymerases is involved in metal-specific catalysis. William C. Cope- land and Teresa s.-F. Wang

11041 Fidelity studies of the human DNA polymerase a. The most conserved region among a-like DNA polymerases is responsible for metal-induced infidelity in DNA syn- thesis. William C. Copeland, Naomi K. Lam, and Teresa S.-F. wang

11050 A human cDNA corresponding to a gene overexpressed during cell proliferation encodes a product sharing ho- mology with amoebic and bacterial proteins. Marie-Thirese Prospkri, Didier Ferbus, Isabelle Karczinski, and Girard Goubin

11125 Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) represses transcription from the promoter of the gene for ornithine transcarbamylase in a manner antagonistic to hepatocyte nuclear factor-4 (HNF-4). Akira Kimura, Atsushi Nishiyori, Takashi Mura- kami, Toshiro Tsukamoto, Shingo Hata, T a b h i Osumi, Ry- oichi Okamura, Masataka Mori, and Masaki Takiguchi

11143 Preparation and characterization of a set of deoxyoli- gonucleotide 49-mers containing site-specific cis- syn, trans-syn-I, (6-4), and Dewar photoproducts of

John-Stephen Taylor thymidylyl(3’ + 6‘)-thymidine. Colin A. Smith and

11160 Cis-active elements controlling lung cell-specific expression of human pulmonary surfactant protein B gene. Robert J . Bohinski, Jacquelyn A. Huffman, Jeffrey A. Whitsett, and David L. Lattier

11172 Comparative analysis of two alternative first exons reported for the mouse osteopontin gene. Elke I. Behrend, Ann F. Chambers, Sylvia M. Wilson, and David T. Denhardt

11199

11222

11247

11312

11326

11356

11364

11372

11389

11409

11426

A novel RNA polymerase 111 transcription factor frac-

gw Dieci, Laura Duimw, Federico Coda-Zabetta, Karen U. tion that is not required for template commitment. Gior-

Sprague, and Simone Ottonello

A mutational analysis of the polypyrimidine tract of

tracts on splicing. Robert F. Roscigno, Mich~el Weiner, and introns. Effects of sequence differences in pyrimidine

Mariano A. Garcia-Blanc0

Phosphorylation weakens DNA binding by peptides containing multiple “SPKK” sequences. George R. Green, Hyuck-Jin Lee, and Dominic L. Poccia

cludes two protein-binding sites in the promoter region. Structure of the canine pancreatic colipase gene in-

George A. Scheele Shin-Ichi Fukuoka, Dong-Er Zhang, Yukw Taniguchi, and

Molecular analysis of the essential gene for adenylate

pornbe.. Manfred Konrad kinase from the fission yeast Schizosaccharomyces

Sequence and functional characterization of a third

pressed in pancreatic islets, kidney, gastrointestinal inositol trisphosphate receptor subtype, IP3R-3, ex-

tract, and other tissues. Olivier Blondel, Jun Takeda, Heil- wig Janssen, Susumu Seino, and Graeme I . Bell

Mapping regions of yeast transcription factor IIIA re- quired for DNA binding, interaction with transcription factor IIIC, and transcription activity. Catherine A. Milne and Jacqueline Segall

A closely linked complex of mouse mast cell-specific chymase genes on chromosome 14. Mich0el.F. Gurish, Joseph H. Nadeau, Kenneth R. Johnson, H. PatrEk McNeil, Kathleen M. Grattan, K. Frank Austen, and Richard L. Stevens

cleosomes in vitro. David John Tremethick and Horace R. High mobility group proteins 14 and 17 can space nu-

Drew

Translational repression of endogenous thymidine ki- nase mRNA in differentiating and arresting mouse cells. Martin Knhfler, Christian Waltner, Erhard Wintersber- ger, and Ernst W. Mullner

Regulatory sequences on the human villin gene trigger the expression of a reporter gene in a differentiating HT29 intestinal cell line. Sylvw Robine, Chrlstian Sahu- quillo-Merino, Daniel Louvard, and Eric Pringault

11440

11449

11456

11463

11474

-10705

10728

10760

10766

10773

10881

10895

10900

10969

.11 073

119

152

278

Organization and promoter activity of the mouse syn- decan-1 gene. Michael T. Hinkes, Olga A. Goldberger, Paul E. Neumnnn, Robert Kokenyesi, and Merton Bernfield

Aldehyde dehydrogenase-derived Q-crystallins of squid

Zinovieva, Stanislav I. Tomarev, and Joram Piatigorsky and octopus. Specialization for lens expression. R i m D.

Mouse procathepsin L lacking a functional glycosyla- tion site is properly folded, stable, and secreted by NIH 3T3 cells. Susan E. Kane

Sequences in the 3’-untranslated region of the human cellular glutathione peroxidase gene are necessary and sufficient for selenocysteine incorporation at the UGA codon. Qichang Shen, Fong-Fang Chu, and Peter E. Newburger

Overexpression in Escherichia coli and affinity puri- fication of chick kidney ferredoxin. Vol. 268 (1993) 5069-5076. Chilung Tang and Helen L. Henry

PROTEIN CHEMISTRY AND STRUCTURE Evaluation of mutagenesis for epitope mapping. Struc- ture of an antibody-protein antigen complex. Lata Pra- sad, Sadhuna Sharma, Margaret Vandonselaar, J . Wilson

Zbignww Dauter, and Louls T. J . Delbaere Quail,, Jeremy S. Lee, E. ,Bruce Waygoad, Keith S. Wilson,

Crystal structure of liganded and unliganded forms of bovine plasma retinol-binding protein. Giuseppe Zanotti, Rodolfo Berni, and Hugo L. Monaco

Refolding of luciferase subunits from urea and assem- bly of the active heterodimer. Evidence for folding intermediates that precede and follow the dimerization step on the pathway to the active form of the enzyme. Miriam M. Ziegler, Michel E. Goldberg, Alain F. Chaffotte, and Thomas 0. Baldwin

Contribution of folding steps involving the individual subunits of bacterial luciferase to the assembly of the active heterodimeric enzyme. Thomas 0. Baldwin, Miriam M. Ziegler, Alain F. Chaffotte, and Michel E. Goldberg

Folding of bacterial luciferase involves a non-native

tive enzyme and the unfolded subunits. A. Clay Clark, heterodimeric intermediate in equilibrium with the na-

James F. Sinclair, and Thomas 0. Baldwin

Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc. Dieter Reinhardt, Karlheinz Mann, Roswitha Nischt, Jay W. For, Man-Li Chu, Thomas Krieg, and Rupert Timpl

Identification of a novel subunit of the Type I inter- feron receptor localized to human chromosome 21. Oscar R. Colamonici and Paul Domanski

Structure-function studies of the human thyrotropin receptor. Inhibition of binding of labeled thyrotropin

C. Morris, Elizabeth R. Bergert, and Daniel J . McCormlck (TSH) by synthetic human TSH receptor peptides: John

tional domains of chicken gizzard smooth muscle cal- Binding and regulatory properties of expressed func-

desmon. Charles S. Redwood and Steven B. Marston

Characterization of glycoprotein I1 from bovine adre-

components representing the secretory vesicle counter- nal medullary chromaffin granules. Identification of

parts of the lysosomal-associated membrane glycopro- teins (lamp-1 and lamp-2). A. David Hieber and David L. Chrktie

A role for cholecystokinin-stimulated protein tyrosine phosphorylation in regulated secretion by the pan- creatic acinar cell. Manfred P. Lutz, Shari L. Sutor, Robert T. Abraham, and Laurence J. Miller

N-Linked glycosylation of the ligand-binding domain of the human urokinase receptor contributes to the affinity for its ligand. Lisbeth Birk Mdller, Jari Wllunen, Ebbe Rdnne, Nina Pedersen, and Francesco Blasi

Symmetrical disulfide bonds are not necessary for as- sembly and secretion of human fibrinongen. Jian-Zhong Zhang, Bohdan Kudryk, and Colvin M. Redmnn

Indicates a Communication

ix

11335 FK-606-binding protein: three-dimensional structure of the complex with the antagonist L-686,818. Joseph W. Becker, Jennifer Rotonda, Brian M. McKeever, H. Karen Chan, Alice I. Marcy, Greg Wiederrecht, Jeffrey D. Hermes, and James P. Springer

11348 Three-dimensional structures of the periplasmic lysine/ arginine/ornithine-binding protein with and without a ligand. Byung-Ha Oh, Jayvardhun Pandit, Chul-Hee Kang, Kishiko Nikaido, Sabihu Gokcen, Gwvannu Ferro-Luzzi Ames, and Sung-Hou Kim

11380 Oxidoreductive regulation of nuclear factor K B. In- volvement of a cellular reducing catalyst thioredoxin. Takuma Hayashi, Yoshio Ueno, and Takoshi Okamoto

11394 P-selectin is acylated with palmitic acid and stearic

Fujimoto, Eric Stroud, Ralph E. Whatley, Stephen M. Prescott, acid at cysteine 766 through a thioester linkage. Tetsuro

Laszlo Muszbek, Michael Laposata, and Rodger P. McEver

11474 Cuticle surface proteins of wild type and mutant Cae- norhabditis elegans. Vol. 268 (1993) 6600-6609. Mark L. Bluxter

Abraham, Robert T., 11119

Adamik, Ronald, 10820 Accili, Domenico, 11272

Ahmed, Maqbool, 11086 Ahmed, Sohail, 10709 Akanuma, Yasuo, 11167 Al-Awqati, Qais, 11283 Alksne, Lefa E., 10813 Alon, Noa, 11304 Ames, Giovanna Ferro-Luzzi,

An, Gang, 10977 A d , Shohji, 11167 Austen, K. Frank, 11372 Backer, Jonathan M., 11256

Baldwin, Thomas O., 10760, Bahnson, Tristram D., 10808

Barlat, Isabelle, 11113 Baron, VBronique, 11272 Baudouy, Nicole, 11113 Becker, Joseph W., 11335 Behrend, Elke I., 11172

Bergert, Elizabeth R., 10900 Bell, Graeme I., 10721,11356

Berman, Jonathan D., 11057 Bernfield, Merton, 11440 Berni, Rodolfo, 10728 Berthiaume, Luc, 10826 Berton, Giorgio, 11321 Beat, Anthony, 10709 Biden, Trevor J., 11065 Bjorkhem, Ingemar, 11079

Blaxter, Mark L., 11474 Blasi, Francesco, 11152

Blondel, Olivier, 11356 Bohinski, Robert J., 11160 Bommineni, Vasudeva R.,

Borsch, Carina M., 11086 Bremer, Hans, 10851

Brownbridge, Geoffrey G., Brendle, James, 11057

Cabrini, Giulio, 11321 Cantrell, Doreen A., 10780 Chaffotte, Alain F., 10760,

Chakrabarti, Debopam, 10796 Chambers, Ann F., 11 172 Chan, H. Karen, 11335 Chang, Xiu-Bao, 11304 Chattejee, Nabendu, 10796 Chen, Anjun, 11002 Chevallier-Multon.

11348

10766,10773

11265

10914

10766

Chowdhury, Jayanta Roy, Marie-Christine: 11113

11265 Chowdhury, Namita Roy, 11265

Christie, David L., 11073

Chu, Mon-Li, 10881 Chu, Fong-Fong, 11463

Chuu, Yue-Jen, 10977 Clark, A. Clay, 10773

Coda-Zabetta, Federico, Clark, Ronald W., 11230

Colamonici, Oscar R., 10895 11199

Constantinescu, Anastasia,

Copeland, William C., 11028,

Crabb, John W., 10863

10906

11041

Damuni, Zahi, 11193 Dauter, Zbigniew, 10705 Davison, Aidan G. M., 11065

Dechecchi, Maria Cristina, Dawson, Sally J., 10739

Delbaere, Louis T. J., 10705 Denhardt, David T., 11172 Denslow, Nancy D., 10796 Dieci, Giorgio, 11199 Dionne, Vincent E., 10808 Dolais-Kitabgi, Jacqueline,

Domanski, Paul, 10895 Drew, Horace R., 11389

Duimio, Laura, 11199 Duchesne, Marc, 11113

Emerich, David W., 10746 Edwards, John C., 11283

Feener, Edward P., 11256 Fath, Isabelle, 11113

Ferbus, Didier, 11050 Fernhndez-Checa, J o d

Carlos, 10836 Flay, Nina, 11 105 Forge, Vincent, 10953,10961 Fox, Jay W., 10881 Fujimoto, Tetsuro, 11394 Fukui, Yasuhisa, 11167 Fukuoka, Shin-Ichi, 11312

Gallyas, Ferenc, Jr., 11208

Garcia-Blanco, Mariano A., Gao, Guang, 10863

Gautier, Nadine, 11272 Gazit, Aviv, 11134 Gilon, Chaim, 11134 Gokcen, Sabiha, 11348 Goldberg, Michel E., 10760,

Goldberger, Olga A,, 11440 Gordon, David A., 11230 Goubin, GBrard, 11050 Gout, Ivan, 10780 Grattan, Kathleen M., 11372 Green, Fiona, 10739 Green, George R., 11247

Guan, Kun-Liang, 11435 Greenberger, Lee M., 11417

Guffanti, Arthur A., 11296 Guillain, Florent, 10953,

Guo, Hong, 11193

Gupta, Swati, 10796 Gupta, Naba K., 10796

Gurish, Michael F., 11372 Hagey, Lee R., 11239 HaBlacher, Meinhard, 10946 Halpern, Jane L., 11188

Han, Derick, 10906 Hamsten, Anders, 10739

Hanrahan, John W., 11304

11321

11272

11222

10766

10961

AUTHOR INDEX

Hata, Shingo, 11125 Harayama, Shigeaki, 10842

Hattori, Seisuke, 11167 Haun, Randy S., 10820 Hayakawa, Makio, 11290 Hayashi, Takuma, 11380 Henney, Adriano M., 10739 Henry, Helen L., 11474 Heppel, Leon A., 10789 Hermes, Jeffrey D., 11335 Hernandez, V. James, 10851 Hidaka, Michihiro, 10754 Hieber, A. David, 11073

Hinkes, Michael T., 11440 Hileman, Ronald E., 10796

Hofmann, Alan F., 11239 Holmberg-Betsholtz, Ingrid,

Holz, Ronald W., 10983

Hori, Takamitsu, 11290 Homma, Yoshimi, 10754

Hou, Yue-Xian, 11304 Huang, Ning-na, 10789 Huffman, Jacquelyn A.,

Humphries, Steve, 10739 Hurwitz, Jerard, 11008,11018 Ishida, Nobuhiro, 11290 Ito, Fumiaki, 11290 Itoh, Naoto, 10932 Ivessa, Andreas S., 10946 hey, D. Mack, 11296 Jakoby, William B., 10870 Janssen, Heilwig, 11356 Jensen, Timothy J., 11304 Johnson, Kenneth R., 11372 Kaburagi, Yasushi, 11167 Kadowaki, Takashi, 11167 Kahn, C. Ronald, 11256 Kaliman, Perla, 11272 Kane, Susan E., 11456 Kang, Chul-Hee, 11348

Karczinski, Isabelle, 11050 Kaplowitz, Neil, 10836

Karpel, Rachel, 11296

Kato, Seisbi, 11217 Kartner, Norbert, 11304

Kearns, Ann E., 11097, 11105 Keefer, Jeffrey R., 11340 Kennard, Olga, 10701 Kim, Nam-Soon, 11217 Kim, Sung-Hou, 11348 Kimura, Akira, 11125

Kinzy, Terry G., 10796 King, George L., 11256

Knofler, Martin, 11409

Kokenyesi, Robert, 11440 Kohlwein, Sepp D., 10946

Konishi, Yoshihiro, 11208 Konrad, Manfred, 11326 Kozma, Robert, 10709 Krieg, Thomas, 10881 Krulwich, Terry A., 11296

Kwon, Young-Guen, 10713 Kudryk, Bohdan, 11278

Lam, Naomi K., 11041 Laposata, Michael, 11394 Lattier, David L., 11160

11079

11160

Lauraeus, Marko, 11470

Lawrence, David S., 10713 Law, Susan F., 10721

Lee, Hyuck-Jin, 11247 Lebrun, Christine, 11272

Lee, Jeremy S., 10705 Lee, Joel, 10709 Lee, Tae Ryong, 10713 Lehotzky, Attila, 10888 Levitzki, Alexander, 11134 Li, Chingwen, 11090 Liliom, Karoly, 10888 Lim, Louis, 10709

Loftus, Anne, 11188 Limbird, Lee E., 11340

Lombardo, Christian R.,

Lotz, Margaret M., 11401 Louvard, Daniel, 11426 Low, Philip S., 10990 Lowe, Peter N., 10914 Lund, Erik, 11079 Lutz, Manfred P., 11119 Ma, Jing Tyan, 10802 Mann, Karlheinz, 10881 Marcy, Alice I., 11335 Marquardt, Hans, 10876 Marston, Steven B., 10969 Masuda, Seiji, 11208 Matsuda, Michiyuki, 11167 Matthies, Heinrich J. G.,

Matuoka, Koozi, 11167

McEver, Rodger P., 11394 McCormick, Daniel J., 10900

McKeever, Brian M., 11335 McNeil, H. Patrick, 11372 Mendelow, Marianne, 10713 Mercurio, Arthur M., 11401 Merrick, William C., 10796 Middleton, Wilbert, 11057 Miller, Laurence J., 11119 Miller, Richard J., 11176

Mintz, Elisabeth, 10953, Milne, Catherine A., 11364

Mdler, Lisbeth Birk, 11152

Monfries, Clinton, 10709 Monaco, Hugo L., 10728

Moore, Keith J. M., 10914 Moore, Dexter S., 11090

Mori, Masataka, 11125 Moriyama, Ryuichi, 10990 Morris, John C., 10900 Moss, Joel, 10820 Muallem, Shmuel, 10997 Mullner, Ernst W., 11409 Murakami, Takashi, 11125 Murakami, Yota, 11008,

Muszbek, Laszlo, 11394 Nadeau, Joseph H., 11372 Nagao, Masaya, 11208

Nakanishi, Osamu, 10754 Nagata, Shigekazu, 10932

Niray-Fejes-Tbth, Anik6,

Neumann, Paul E., 11440 Newburger, Peter E., 11463

10990

11176

10961

11018

10717

Neyfakh, Alexander A., 11086 Nikaido, Kishiko, 11348 Nischt, Roswitha, 10881 Nishiyori, Atsushi, 11125 Noguchi, Tetsuya, 11167 Oh, Byung-Ha, 11348 Ohmura, Satoshi, 11217

Okamura, Ryoichi, 11125 Okamoto, Takashi, 11380

Ookhtens, Murad, 10836 Oku, Naoto, 11290

Osherov, Nir, 11134 Osterman, John C., 10796 Osumi, Takashi, 11125 Ottonello, Simone, 11199 Ovadi, Judit, 10888

Packer, Lester, 10906 Padan, Etana, 11296 Palfrey, H. Clive, 11176 Paltauf, Fritz, 10946 Pan, Rong Long, 10802 Pandit, Jayvardhan, 11348 Pandol, Stephen J., 10808 Parker, Rex A., 11230 Parsels, Leslie A,, 10983 Pearce, Bradley C., 11230 Pedersen, Nina, 11152 Piatigorsky, Joram, 11449 Pinner, Elhanan, 11296 Pluskey, Scott, 10713 Poccia, Dominic L., 11247 Polliinen, Jari, 11152 Poulter, C. Dale, 11002 Prasad, Lata, 10705 Prescott, Stephen M., 11394 Pringault, Eric, 11426 Prospki, Marie-ThBrhse,

Prugue, Monica L., 11065 Quail, J. Wilson, 10705

Ramaswamy, Sengoda, 10870 Ray, Prabhati, 11057 Reddy, Shrikanth A. G.,

Redman, Colvin M., 11278

Reed, John C., 10920 Redwood, Charles S., 10969

Reinhardt, Dieter, 10881 Reif, Karin, 10780

Reisine, Terry, 10721

Riordan, John R., 11304 Rekik, Monique, 10842

Robine, Sylvie, 11426 Rdnne, Ebbe, 11152

Rosenberg, Robert C., 11090 Roscigno, Robert F., 11222

Rosenberrv, Terrone L..

11050

11193

Rotonda, Jennifer, 11335 Rusvai, Erzsebet, 10717

10938

Sahuquillo-Merino, Christian, 11426

Saito, Koichi, 10920

Salisbury, Stephen A,, 10701 Salerno, Allen, 10713

Sasaki, Ryuzo, 11208 Scarpetta, Marco A,, 10927