Variety Release Proposal Early 2012

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    Proposal for the Release of Arun-4, Arun-3 and Karnali-2

    Submitted by

    National Maize Research Program (NMRP), Rampur, Chitwan, Nepal

    Proposed by

    KB Koirala, J Shrestha, CB Kunwar, DB Gurung, TR Rijal, BH

    Adhikary

    ,

    SR Upadhyay

    , DC Paudel

    , GB Hamal

    , P Thakur

    , BBhandari

    , KP Dhital

    , IP Upadhyay

    , GS Bhandari

    , RK Rajak

    , CL

    Chaudahry

    , N Dhami, YR Pandey

    Y, A Pokhrel

    u, HK Prasai

    x, RB

    KatuwalM

    and MP Tripathi

    A Joint Accomplishment of:National Maize Research Program (NMRP), RampurRegional Agriculture Research Station (RARS), Parwanipur

    Regional Agriculture Research Station (RARS), NepalgunjAgriculture Botany Division (ABD), Khumaltar

    YRegional Agriculture Research Station, Lumle

    uAgriculture Research Station, Dailekh

    MAgriculture Research Station, Pakhribas

    xAgriculture Research Station, Doti

    March, 2012

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    Application to be made for release of new maize varieties

    Government of Nepal

    Ministry of Agriculture and Cooperatives

    National Seed Board

    Format for submission of proposal for the release ofArun-4,Arun-3andKarnali-2 maize varieties

    The Variety Approval, Release and Registration Sub-committee (VARRC)National Seed Board Secretariat

    Seed Quality Control CentreHarihar Bhawan, Lalitpur

    Tel. +977-01-5521359

    Sub: Application for the release of maize variety Arun-4, Arun-3 and Karnali-2

    As, we have bred the seeds of new variety described below, we hereby make this application

    for the release of the seeds of the variety.

    Original

    entry name

    Suggested name

    English Nepali

    Arun-4 Arun-4 c?0f$Arun-1 EV Arun-3 c?0f#Pool-17 E Karnali-2 s0ff{nL@

    1. Background information

    Maize is the 2nd

    most important lifesaving crops of the country with the potentiality of

    highest productivity per unit area. It is the food for more than 14 million people in the hills

    and playing a vital role in the livelihood of rural people in Nepal. The average nationalproductivity of maize in Nepal is 2119 kg/ha (MoAC, 2009/10) which is very low compared

    to maize productivity in India and China, the neighboring countries. Mid hill region is themost important corn belt of the country where more than 70% maize area of the country is

    situated (MoAC 2009/10).

    The exponential growth of the human population and change of food habit in the recent

    years, people are consuming more milk, meat, fish and eggs because of increasing trends of

    income every year. The increased demand of those items needs more maize grains for

    poultry and animals feeds. Maize can be used as food, feed, fodder, fuel and raw materials

    for various industrial purposes. Maize is the main ingredient for poultry and animal feeds.

    The annual growth rate of demand for feed will be 3.38 per cent per annum for 2001 to

    2010. Meanwhile, imports of maize seeds have also risen. Maize seed imports soared to

    9,778 tons in 2009-10 from 458 tons during the previous fiscal year (NMRP, 2011). Maize

    growing farmers have not faced any problems of selling maize grains in Nepal.

    Increment in the production and productivity of maize in the country is extremely essentialwhich is possible only by developing and promoting new and improved high yielding maize

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    varieties. The proposed varieties namely Arun-4, Arun-3 and Karnali-2 are of broad geneticbased having good yield potential. Stakeholders preferred these varieties due to higher grain

    yield, non-lodging and moderately resistant to weevil and stem borer. It is equally good forintercropping and green ear roasting. The mean grain yield of Arun-4, Arun-3 and Pool-17 E

    varieties were tested by majority of marginalized and socially disadvantaged groups of

    farmers (Dalit and women) and they expressed their desire to plant these varieties in thecoming years.

    Existing varietal options to the farmers is very low and varietal replacement rate of the

    country is also poor. Seven released maize varieties have been denotified out of the total

    twenty three released varieties in the country. In this context, we should provide more

    varietal selection options to the maize farmers to enhance the productivity of maize in terai

    and hills. The newly proposed three maize varieties namely Arun-4, Arun-3 and Karnali-2

    could be able to provide immediate varietal selection options to the farmers. Since these

    varieties are early in maturity days, they fit in rice-wheat-maize cropping system and

    vegetables can be grown after harvesting them. Keeping all these in consideration, NMRP

    has proposed three new maize varieties namely Arun-4, Arun-3 and Karnali-2.

    2. General Information2.1. Common name: Maize

    2.2. Botanical name:Zea mays L2.3. Original designation/Symbolic name used in testing: Arun-4, Arun-1EV and Pool-

    17 E

    2.4. Pedigree: Arun-4 (formed using elite introduced germplasm and local landraces),

    Arun-1EV and Pool-17 E (comprised of crosses among the early and late flint

    materials from mexico, the Caribean, Central and South America and Asia)

    2.5 Cross Parents:-

    2.6. Country of Origin: Nepal2.7. Source of material (farmer/community, name of the experiment and the year in

    which it was first introduced): CIMMYT

    2.8. Years and location of testing of the variety in Nepal

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    Table 1. Summary of the trials conducted through NMRP, Rampur in 2005-2010

    Year Experiments Locations References

    2006 CFFT Rajahar and Sukranagar Annexure 1

    2010 CFFT Sukranagar, Rajahar and

    Manahari

    Annexure 2

    2003 CVT Rampur Annexure 32003 CFFT Lumle, Pakhribas and Dailekh Annexure 4

    2003 CFFT Lumle, Pakhribas , Dailekh andKabre

    Annexure 5

    2006 CVT Rampur Annexure 62006 CFFT Dailekh, Pakhribas and

    Khumaltar

    Annexure 7

    2007 CFFT Sukranagar Annexure 8

    2007 CFFT Rajahar Annexure 9

    2007 CFFT Manahari Annexure 102006 CVT ARS Doti Annexure 11

    2006 Variety cum density trial Rampur Annexure 122009 CFFT Pakhribas Annexure 15

    2005 CVT Dailekh, Lumle and Pakhribas Annexure 16

    2005 CFFT Dailekh, Lumle and Pakhribas Annexure 17

    2005 CFFT Dailekh, Lumle and Pakhribas Annexure 18

    2006 CVT Dailekh, Pakhribas and Lumle Annexure 19

    2010 Heat screening trial Rampur, Nepalgunj and

    Parwanipur

    Annexure 20

    2009 Disease screening trial Rampur Annexure 21

    2010 Heat screening trial Rampur, Nepalgunj and

    Parwanipur

    Annexure 22

    2009 Insect screening nursery Rampur Annexure 23

    2010 Insect screening nursery Rampur Annexure 242010 CFFT Pakhribas Annexure 25

    2010 CFFT Chambas Annexure 262005 CFFT Rajahar Annexure 27

    2001 On farm evaluation trial Syangya and Palpa Annexure 282006 CFFT Dailekh, Annexure 29

    2004-06 CVT Pakhribas Annexure 30

    2008 Economic analysis of

    intercropping

    Rampur Annexure 31

    2010 Disease and insect screening Rampur Annexure 322010 Morphology trial Rampur Annexure 33

    2011 CFFT Dailekh Annexure 34

    2010 CFFT Chambas Annexure 35

    2.9. Farmers Field Trial (FFT), participatory trial data/information and stakeholders

    preferences: Arun-4 is the number one preferred early variety by farmers. Similarly Arun-

    1EV is third preferred variety after Arun-2 in Chambas, Tanahun (Annex 33).

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    3. Summary of Varietal Characteristics

    3.1 Agronomic

    Arun-4:This is an early maize variety and has yield potential up to 4436 kg ha

    -1. Kernel is yellow

    and flint type. Plant stature is similar to Arun-2. Its open leaves, stem is robust and has nolodging problem. This variety can be grown in the winter seasons in terai and inner terai and

    mid hill regions. However, main growing season is summer season.

    Table 2: Major agro-morphological traits of Arun-4

    Parameters Mean SD Range

    Maturity days 113-116 -

    Days to 50% anthesis 54.084.04 47-62.5

    Days to 50% silking 57.873.81 52-66.5

    Plant height (cm) 200.3225.78 143-247Ear height (cm) 107.2118.06 73-140.3

    Grain yield (kg ha-1) 4436924 2342-6050

    Arun-1 EV:

    Arun-1EV is a early maize variety and has yield potential up to 4042 kg ha-1

    . Kernel is

    white and flint type. Plant stature is similar to Arun-2. Its open leaves and short plant height

    offers high density planting. Stem is robust and has no lodging problem. This variety can be

    grown in the winter seasons in terai and inner terai and mid hill regions. However, main

    growing season is summer season.

    Table 3: Major agro-morphological traits of Arun-1 EV

    Parameters Mean SD Range

    Days to maturity 100 -Days to 50% anthesis 54.464.11 47-62.25

    Days to 50% silking 57.84.09 50-66.75Plant height (cm) 194.0427.18 152-238

    Ear height (cm) 104.8824.11 68-142.5Grain yield (kg ha-1) 4042932 2395-5904

    Pool-17 E:This is an early maize variety and has yield potential up to 3555 kg ha -1. Kernel is yellow

    and flint type. Its open leaves and short plant height offers high density planting. Stem isrobust and has no lodging problem. This variety can be grown in the winter seasons in terai

    and inner terai and mid hill regions. However, main growing season is summer season.

    Table 4: Major agro-morphological traits of Pool-17 E

    Parameters Mean (both season) SD Range

    Days to maturity 82 -

    Days to 50% anthesis 50.933.42 45-56

    Days to 50% silking 53.863.45 47-59

    Plant height (cm) 182.6118.84 146-209

    Ear height (cm) 92.4525.26 59-143.3

    Grain yield (kg ha-1

    ) 3555709 2450-47763.2. Response to stresses (please specify):

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    3.2.1 Biotic stresses(a) Insects: Specific problems related to insects have not been identified. It is apparent

    from the results that all Arun-4, Arun-1EV and Pool-17 E showed minimum

    infestation of maize stem borer. Weevil infestation during storage is not a problem

    since the husk cover is tight.(b)Diseases: Arun-4, Arun-1EV and Pool-17 E has shown resistant to moderately

    resistant reaction against leaf blight diseases (Northern leaf blight, Southern leaf

    blight and Banded leaf and sheath blight (BLSB) in natural condition (Annexure 29and 30), though its inbred exhibited moderately susceptible reaction agains t NLB

    under artificially inoculated condition (Annexure 4 and 30).

    (c)Weeds: No specific association of weeds with this variety and does not require anyspecial care for weed management. However, the field should be free from weed to

    attain higher grain yield and crop management practices applied to other maize

    hybrids or OPVs is also sufficient for this variety.

    3.2.2 Abiotic stresses(a) Adverse climatic conditions: These varieties were evaluated under heat screeningnursery at NMRP, Rampur; RARS, Parwanipur; and RARS, Tarahara in summerseason of 2010 (Annex 20). The results showed that it prefer favorable

    environment rather than heat stress condition for higher yield performance. Thecrop could not perform well under any type of stress condition such as water

    stagnation, drought, heat and cold stresses. The genotypes can flourish its

    potentiality under optimum condition.

    (b) Adverse soil conditions: The crop prefers loam to sandy loam soil for better yield

    performance. It requires frequent irrigation and heavy dose of manure and

    fertilizer than OPVs to obtain expected yield.

    (c ) Other stresses, if any:

    3.3. Grain/leaf/root/curd/bulb/seed/fruit/tuber qualities:

    3.3.1 Nutritional quality (if available):Table 5: Nutritional analysis of Arun-4, Arun-1 EV and Pool-17 E

    Traits Arun-4 Arun-1EV Pool-17 E

    Ash (%) 1.2955 1.3349 1.4468

    Moisture (%) 11.4808 11.0359 11.9700

    Fat (%) 4.6437 4.4373 3.9078Protein (%) 9.5500 10.8275 10.9175

    Crude fiber (%) 1.61 1.98 1.89

    Carbohydrates (%) 71.4200 70.3844 69.8679

    Thousand grains weight (gm) 276.3 292.8 252.6Bulk density (gm litre-1) 783.6 799.5 831.0

    (Source: Food Research Unit, 2011)

    3.3.2 Processing quality:Traits Arun-4 Arun-1EV Pool-17 E

    Grit (%) 68.8 69 68.5

    flour(%) 24.5 24.5 24.5bran (%) 6.6 6.5 6.4

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    Grit : Flour Ratio 3.6:1 3.1:1 3:1:1

    3.3.3 Organoleptic test (cooking quality, taste, aroma/flavour, etc): % popping is13.6% (Arun-1EV) and 21.3% (Arun-4) in earthern pot

    3.3.4 Other specific qualities (if any): -3.4. Other characteristics (threshing, storability, market potential etc.):

    4. Morphological characteristics (size, shape, colour, etc of various plant parts):

    Characteristics Arun-1EV Arun-4 Pool-17 E

    Anthocyanin coloration of leaf

    sheath

    Absent/very

    weak

    Absent/very

    weak

    Absent/very

    weakAnthocyanin coloration of brace

    roots

    Strong Strong Strong

    Degree of zigzag of the stem Abs/v. slight Abs/v.slight Medium

    Leaf angle Medium Large Medium

    Leaf attitude Slightly curved Strongly curved Curved

    Angle of tassel branch Short Short Short

    Attitude of tassel branch Slightly curved Slightly curved Slightly curved

    Intensity of anthocyanin

    coloration of glume

    Strong Weak Medium

    Anthocyanin coloration of anthers Strong Weak Weak

    Anthocyanin coloration of silk Absent/v.slight Present Absent/v.slight

    Intensity of anthocyanin

    coloration of silk

    Weak Strong Weak

    Density of spikelets of the tassel Dense Dense DenseType of grain in middle third of

    the grain

    Flint Flint Flint

    Color of grain White Yellow Yellow

    (Source: NMRP 2011. Annual Report 2010/11. National Maize Research Program,

    Rampur, Chitwan, Nepal)

    4.1. Major identifying characteristics of a crop variety for cultivar authenticity:

    The genotype can be distinguished from others maize genotypes by following traits.

    (Source: NMRP 2011. Annual Report 2010/11. National Maize Research Program,Rampur, Chitwan, Nepal)

    Characters Overall mean

    Arun-4 Arun-1EV Pool-17 E

    Leaf number above cob 5.33 5.00 5.00

    Leaf number below cob 8.33 8.00 5.00

    Ear Length, cm 17.6 15.7 16.7

    Ear diameter, cm 13 12.4 14.3

    No. of kernel rows/ear 12.4 13.7 12.8

    No. grain/row 25.6 27.4 25Shelling, % 80 80 81

    Tassel branch number 16.75 18.5 14.6

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    Arun-4: Kernel is yellow and flint type. Open and green leaves, robust stem and

    short plant height. Internodes green not swollenArun-1 EV: Kernel is white and flint type. Open and green leaves, robust stem and

    short plant height. Internodes green and swollen, cob size medium.

    Pool-17 E: Kernel is Yellow and flint type. Open and green leaves, robust and thinand slightly zigzag stem and short plant height, cob size small, leaf width

    small and earliest in maturity

    4.2. Molecular characteristics (Not available)

    5. Recommendation domain:

    5.1Geographical area (altitude, latitude and longitude): Arun-4 and Arun-1EV were testedat many locations from east to mid-western terai in the various experimental trials CVT

    and FFT (Table 1). Therefore, this variety is proposed to recommend for commercial

    cultivation from east terai to mid-western Terai, inner Terai and mid hills of Nepal. 5.2Moisture regime: partially irrigated and irrigated. Soil moisture as well atmospheric

    moisture is critical at 7 days before and after flowering.

    5.3Climatic conditions: If the atmospheric temperature rises more than 38oc pollen grainsstart to die by appearing tassel firing symptoms and atmospheric temperature below 8

    oC

    is not also suitable during flowering time.

    5.4Cropping pattern:The variety is suitable under both maize and rice based cropping system. However,

    higher level of production can be obtained when the crop is grown in winter rather than

    summer planting.

    a. Maize based cropping system:Maize-Soybean-VegetablesMaize-Rajma-Vegetables

    Maize-Sesame-Maize

    Maize-Black gram-tori etc.

    b. Rice based cropping system:Rice-Maize-Mungbean

    Rice-Maize-Cowpea

    Rice-Maize-Vegetables

    Rice-Wheat-Maize

    Maize-Maize-Rice etc.5.5Socioeconomic conditions: The farmers who are growing maize in terai and inner terai

    condition can easily grow this variety. Farmers can use as fodder after harvesting andget more benefits from this variety than others from milk and milk products. So, the

    variety is friendly even to resource poor farmers, who can get higher yield per unit area.

    5.6Economic analysis and benefit:Arun-4:

    The improved genotype Arun-4 yielded 12.35 % more grain yield than the local check when

    averaged over seven years data from CFFT over the locations. The average yield of seven

    years (2001-2010) of Arun-4 was recorded 4436 kg/ha whereas local check was recorded at

    the same period 3888 kg/ha. The difference between this improved one and local check atthe farmers field was 548 kg/ha which amounts Rs 9316 if sold for grain @ Rs 17/kg

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    whereas the same quantity if sold as seed @ Rs 40/kg then, the amount will be of Rs21920/- which is extra benefit by growing this genotype. Under intercropped with soybean,

    Arun-4 produced net income of NRs. 43228/- per hectare (NMRP, 2009)

    Arun 1 EV:

    The improved genotype Arun-1 EV yielded 3.80 % more grain yield than the local checkwhen averaged over seven years data from CFFT over the locations. The average yield of

    seven years (20001-2010) of Arun-1EV was recorded 4042 kg/ha whereas local check was

    recorded at the same period 3888 kg/ha. The difference between this improved one and localcheck at the farmers field was 154 kg/ha which amounts Rs 2618/- if sold for grain @ Rs

    17/- whereas the same quantity if sold as seed @ Rs 40/kg then, the amount will be of Rs

    6160/-, which is extra benefit by growing this genotype. Under intercropped with soybean,

    Arun-1 EV produced net income of NRs. 36640/- per hectare (NMRP, 2009)

    Pool-17 E:The improved genotype Pool-17 E yielded 9.36 % less grain yield than the local check when

    averaged over seven years data from CFFT over the locations. The average yield of sevenyears (20001-2010) of Pool-17 E was recorded 3555 kg/ha whereas local check was

    recorded at the same period 3888 kg/ha. The difference between local check and thisimproved one at the farmers field was 333 kg/ha which amounts Rs 5661/ - if sold for grain

    @ Rs 17/- whereas the same quantity if sold as seed @ Rs 40/kg then, the amount will be ofRs 13320, which is extra loss by growing this genotype. However under intercropped with

    soybean, Arun-1 EV produced net income of NRs. 13008/- per hectare (NMRP, 2009).

    6 Production and management aspect (Please add detailed information, if necessary, onthe general cultivation practices; appropriate input and moisture regimes): Package of

    practice is similar to the Arun-2, released in 1982 and other maize varieties which are

    growing by farmers in maize growing districts.

    6.1Agronomic Practices: 2-3 ploughings are needed for land preparation. First ploughing should be carried out

    30 days prior to maize sowing.

    The main season for planting these varieties are summer season however it can begrown in winter and spring season.

    The recommended dose of fertilizer 120:60:40 N:P2O5:K2O kg ha-1 along with 10-15 tons of farm yard manure.. FYM and full dose of P205 and K20 along with 50%

    nitrogen should be applied during last ploughing (before crop sowing). 25% Urea

    should be top-dressed during knee high stage and 25% just prior to tasseling andsilking.

    Seed rate 25 kg ha-1 for general grain production. The recommended planting space for early variety is : Row-to-Row distance:

    75 cm

    Plant to plant distance: 25 cmWeeding: First weeding should be done within 20-25 days and second weeding (earthing

    up) within 40-45 days after the germination. Priority should be given to manual weeding.However, Glyphosate @ 5ml/litre as pre-planting and Pendimethylene @ 5.5 ml/litre can be

    applied as pre-emergence. Post emergence can be applied with 2-4-D @ 3ml/litre of water.

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    6.1.2 Irrigation: Knee high stage, tasseling and silking, and grain filling stages are mostcritical for moisture stress. Light irrigation should be applied to the maize crop at these

    stages depending on the soil moisture status and irrigation facilities.

    6.1.3 Detasseling: If weather condition is normal (high humidity and temperature less than

    38

    oc

    ), 30% plants can be detasseled, which helps to increase maize production up to 7%.

    6.1.4 Harvesting: Crop should be harvested after physiological maturity. Maize can be

    harvested when black layer is formed on maize grains.

    6.1.5 Drying: Maize ears should be sun dried for 3-4 days and before shelling. Grains

    should be dried on the tarpaulin and covered during the night. When the grain moisture is

    reduced down to 12%, then grains are either stored in metal bins or kept in sealed bags

    after processing of seeds.

    7.Reasons for release

    Arun-4

    Arun-4 is the number one preferred early variety by farmers (Annex 33). Thevigorous growth, early maturing habit, good taste and good husk cover are thepositive aspects of Arun-4 as compared to previously released varieties.

    It is moderately resistant to major maize diseases like Turcicum leaf blight (TLB),Helminthosporium leaf blight (HLB) and Gray leaf spot (GLS). The infestation of

    stem borer in Arun-4 is minimum (Annex 32).

    Arun-4 is highly lodging resistant. Local varieties are highly lodging prone. Thus,Arun-4 addresses the real need of the farmers.

    Mean height of Arun-4, is 200.32 cm which are tall enough to fulfill the need offodder for livestock for the farmers

    Arun-1 EV

    Arun-1EV performs better in farmers field. The vigorous growth, early maturinghabit, good taste and good husk cover are the positive aspects of Arun-1EV as

    compared to previously released varieties.

    It is moderately resistant to major maize diseases like Turcicum leaf blight (TLB),Helminthosporium leaf blight (HLB) and Gray leaf spot (GLS). The infestation ofstem borer is minimum (Annex 32).

    Arun-1 EV is highly lodging resistant. Local varieties are highly lodging prone.Thus, Arun-1 EV addresses the real need of the farmers.

    Pool-17 E

    Since Pool-17 E variety is short in plant height and strong stem, there is no lodgingproblem in this variety.

    Pool-17 E variety escape drought stress during the flowering time because of early inflowering.

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    It is moderately resistant to major maize diseases like Turcicum leaf blight (TLB),Helminthosporium leaf blight (HLB) and Gray leaf spot (GLS).

    Since Arun-4, Arun-3 (Arun-1EV) and Pool-17 E are early in maturity thesevarieties fit in rice-wheat-maize cropping system and vegetables can be grown afterharvesting them.

    Releasing these varieties creates a better opportunity for commercialization in maizeseed production in private sector and could benefit the farmers, seed growers andothers, who are involved in seed production and distribution chains.

    Finally, the release of these varieties will provide varietal choices to the farmers andcontribute to the food security in the mid hills and terai of Nepal.

    8. Seed supply situationNMRP has been maintaining numbers of inbred lines and many released and prereleased

    maize varieties since many years. Although the available seed of these inbred lines and

    hybrids are not sufficient for selling in the market but it can provide sufficient amount of

    source seed for seed multiplication purpose as demanded by the interested stakeholders.

    Table 6: Seed supply situation of inbred lines and hybrids at NMRP, Rampur

    SN Category of seed Amount available (kg) Whereavailable

    Personhandling

    1 Arun-4 Breeder 9 NMRP,

    Rampur

    B Bhandari

    Foundation 202 Arun-1 EV Breeder 6 NMRP,

    Rampur

    B Bhandari

    Foundation 30

    3 Pool-17 E Breeder 7 NMRP,

    Rampur

    B Bhandari

    Foundation 25

    Maintenance of Variety

    NMRP will take the responsibility of maintaining, improving and breeder seed production

    of Arun-4, Arun-1 EV and Pool-17 E

    9. Acknowledgements

    The present work is an outcome of a team and collaborative work in varietal development,which is a part of regular research activities of National Maize Research Program (NMRP),

    Rampur. We are deeply indebted to the NMRP staffs and collaborators, farmers and others

    who are directly or indirectly involved in the development and evaluation phases of these

    varieties

    Pattern of stability for grain yield:

    Yield stability is the ability of a genotype to avoid substantial fluctuations in yield over arange of environments. Varieties with high and stable performance are desirable. So stability

    analysis was performed using regression method;

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    Table 7: Stability pattern of early maize varieties under CFFT early sets at different sevenlocations (Dailekh, Kaski, Dhankuta, Nawalparasi, Chitwan, Makawanpur and Tanahun)

    and in different four years (2005, 2006, 2009 and 2010)

    Genotype

    s

    Grain yield (kg ha-1

    ) Regression

    Coefficient(b)

    Coefficient

    ofDetermination (R2)

    %

    Standard

    Errorof b

    (SEb)

    Yield

    advantageover

    Farmers

    variety

    MeanSEM

    Range ofgrain yield

    Pool-17E

    3572211 2511-4776 0.72** 75.3 0.1244 -10.3

    Arun-1

    EV

    4201287 2395-5904 1.04** 85.3 0.1304 6.2

    Arun-4 4289 275 2342-6050 1.01** 86.5 0.12 8.1

    Arun-2 4294282 2407-5942 1.03** 86.8 0.1212 8.2

    Farmers

    variety

    3941330 1964-5838 1.24** 91.3 0.1155

    Grand

    Mean

    4059.4

    The varieties having regression coefficient (b) nearly equal to 1 was considered stable for all

    environments. The b values significantly less than 1, indicating the constant expression of

    the traits under the range of environments and better adaptation to poor environments. The b

    values more than 1, which indicates their responsiveness to changes in environmental

    conditions and specific adaptation to favorable ones. Therefore, Arun-4 and Arun-1 EV are

    stable varieties for all environments (both mid hills and terai) hence their regression

    coefficients (b) are nearly equal to 1 and these varieties are most suitable for these regions

    because their grain yields are above the average yields. The standard error of mean and

    range of grain yield of Pool-17 E are smallest among the tested varieties. And its regression

    coefficient (b) less than 1 and grain yield is below the average yield, so this variety is stableunder wide range of environments and best alternative of Arun-4 and Arun-1 EV.

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    Figure 17: Stability (joint regression analysis) of Arun-1 EV, Arun-4 and Pool-17 E overFarmers variety and check variety (Arun-2) from coordinated farmers field trial( CFFT)

    cut data (n=11) in 2005, 2006, 2009 and 2010 from Kaski (2), Dhankuta (3), Dailekh (1),Nawalparasi (3), Chitwan (3), Makawanpur (1) and Tanahun (1) districts of Nepal

    Trial mean yield (kg/ha)

    Varietymeangrainyield(kg/ha)

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    10. References

    ARS, Dailekh. 2011. Annual Progress Report 2010/11, Agricultural Research Station,

    Kimugaun, Dailekh

    NMRP 2004. Annual Report 2003/04. National Maize Research Program, Rampur,

    Chitwan, Nepal

    NMRP 2005. Annual Report 2004/05. National Maize Research Program, Rampur,

    Chitwan, Nepal

    NMRP .2006 Annual Report 2005/06. National Maize Research Program, Rampur,

    Chitwan, Nepal

    NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur,

    Chitwan, Nepal

    NMRP 2008. Annual Report 2007/08. National Maize Research Program, Rampur,Chitwan, Nepal

    NMRP 2010. Annual Report 2009/10. National Maize Research Program, Rampur,

    Chitwan, Nepal

    NMRP 2010. Annual Report 2009/10. National Maize Research Program, Rampur,

    Chitwan, Nepal

    NMRP 2011. Annual Report 2010/11. National Maize Research Program, Rampur,Chitwan, Nepal

    Pandey MP, RP pandey and D Sharma.2002. Farmers assessment of improved maize

    varieties in the hills of western Nepal. In: NP Rajbhandari, JK Ransom, K Adhikari

    and AFE Palmer (eds) 2002. Sustainable Maize Production Systems for Nepal:

    Proceedings of a maize Symposium held , December 3-5, 2001, Kathmandu,

    Nepal:NARC and CIMMYT.pp105-107

    RARS, Lumle. 2011. Annual Report. 2010/11. Regional Agricultural Research Station,Lumle, Kaski, Nepal

    Sah SN, RB Katuwal, RK Mahato, D Sharma, SR Upadhyay and A Jha.2007. Varietal

    Research on Early Maize, Normal and Quality Protein Maize (QPM) for theEastern Mid Hills of Nepal. Pp.185-191. Gurung DB, DC Paudel, G K.C., SR

    Upadhyay and BB Pokhrel (eds). Proceedings of the 25th National Summer Crops

    Workshop on Maize Research and Production in Nepal held June 21-23, 2007

    Kathmandu, Nepal.National Maize Research Program, Rampur, Chitwan, Nepal.

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    Proposed by

    Signature:

    Name: Dr. Keshab Babu Koirala

    Main proposing organization: National Maize Research Program (NMRP), Rampur

    Designation: National Maize Coordinator/Senior Scientist S4

    Corresponding address: National Maize Research Program (NMRP), Rampur, Chitwan

    Tel. +977-56-591001

    Fax: +977-56-592548

    Mobile: +9779851140616

    Email:[email protected]

    Website if applicable:

    mailto:[email protected]:[email protected]:[email protected]:[email protected]
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    Annexes

    Annex 1: Mean grain yield, of early maize genotypes CFFT, 2006

    SN GenotypesGrain yield (t/ha)

    Rajahar Sukranagar

    1 Arun-1 EV 4.37 4.14

    2 Arun-4 4.84 4.823 Pool-17 E 3.94 3.70

    4 Arun-2 4.47 4.22

    5 Farmers variety 5.40 4.00

    Grand Mean 4.61 4.171

    F-test * *

    CV% 17.19 12.29

    LSD (0.05) 0.953 0.617

    Notes: CV = Coefficient of variation, F-test=Fishers test, LSD = Least significant difference of means, *= significant

    (Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

    (Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 3 : Summary of mean grain yield and agronomic traits of early and extra early maize genotypes in CVTE at NMRPRampur, 2003 winter.

    E.N.

    Genotypes Floweringdays

    Height (cm) Plants/ha(000)

    Ear/ha(000)

    PlantAsp.(1-5)

    EarAsp.(1-5)

    Huskcov

    (1-5)

    GrainYield(t/ha)Male Femal Plant Ear

    1 S97TEYGHAYB (2) 55 58 193 97 52.00 47.56 1.3 2.17 2.0 4.81

    2 Arun-1 EV 54 56 217 115 54.67 46.67 2.3 1.83 2.0 3.923 Pool-27 51 53 203 108 57.78 43.56 2.0 2.0 1.5 4.52

    4 Zm-301 52 58 148 80 44.90 40.44 2.7 2.3 2.2 3.24

    5 S97TEYGHAYB (3) 60 64 195 105 55.56 47.11 1.5 1.67 1.67 4.25

    6 Pool-16 50 52 180 78 50.67 45.33 2.3 2.83 2.0 3.48

    7 PL15QC7SRC-F2 56 59 207 92 54.67 49.78 1.8 2.50 1.83 4.43

    8 Z97EWB-F2 52 55 200 102 46.22 47.11 2.3 2.33 1.67 3.629 Arun-4 52 54 198 97 46.22 47.11 2.5 2.0 2.0 3.86

    10 Arun-2 (Std. Check) 51 54 225 118 56.44 53.33 2.3 2.33 2.0 4.94

    11 Farmers Variety 60 64 225 130 52.89 46.22 1.8 1.67 1.17 4.99

    MeanF test

    CV %LSD (0.05)

    54**

    3.93.59

    56**

    4.874.68

    199**

    8.5629.03

    102**

    13.323.2

    52**

    7.276.44

    47ns

    11.429.09

    2.09 2.15 1.82 4.18*

    14.861.06

    (Source: NMRP 2004. Annual Report 2003/04. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 2: Grain yield and other traits of Maize CFFT Early maize at OR sites Shukranagar, Rajahar & Manahari in 2010summer

    Shukranagar Rajahar Manahari

    Genotypes No. of Plants/ha

    No. ofof ears/ha

    GYkg/ha

    No. ofPlants/ha

    No.ofEars

    /ha

    GYKg/ha

    No. ofPlants/ha

    No. ofEars/ha

    GYkg/ha

    Pool-17 E 45667 47333 4594 42667 41333 2704 46000 43333 3723

    Arun-1 EV 49000 51167 5904 41333 42000 2562 44000 42667 4046

    Arun-4 48167 49667 6050 41333 40000 3413 50000 48000 5291

    Arun-2 47333 46167 5942 44000 42667 3282 44667 44000 4423

    Farmers variety 42667 44333 5838 44000 44000 1964 46000 43333 4428

    Grand mean 46567 47733 5660 42667 42000 2797 46133 44267 4382

    F-test * ** * ns ns * * ns *

    CV % 5.95 4.78 11.71 8.84 8.92 9.07 3.42 5.83 7.8LSD (0.05) 4270 3517 1023 10470 10404 704 4380 7168 949

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    Annex 4: Mean grain yield and agronomic traits of early and extra early maize genotypes in CFFTE pooled over locations(LAC, PAC, Dailekh), 2003 Summer.

    S.N. Genotypes Flowering days Height (cm) Plants/ha (000) Ear/ha(000)

    Grain Yield(t/ha)Male Female Plant Ear

    12

    3

    456

    Pool-15 EPool-17 E E

    Pool-16

    Arun-4Arun-2 (Std. Check)Farmers Variety

    4951

    53

    525255

    5355

    58

    565659

    186204

    213

    228227250

    8383

    138

    111116144

    3441

    44

    464444

    3339

    41

    454242

    2.65 C3.60 BC

    4.30 AB

    4.99 A4.85 AB4.10 AB

    Grand meanF-testCV %

    LSD (0.05)

    52*

    3.79

    3.58

    56*

    3.58

    3.66

    218*

    10.04

    39.84

    113ns

    26.43

    -

    42ns

    13.55

    -

    40ns

    13.62

    -

    4.10*17.05

    1.26

    (Source: NMRP 2004. Annual Report 2003/04. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 5: Performance of early maize genotypes in CFFTE, pooled over hills locations (Pakhribas, Dailekh, Lumle,

    Kabre),2003 summer

    Genotypes Days to 50% Flowering Plant ht.

    (cm)

    Ear ht.

    (cm)

    Plant/ha

    (000)

    ears/ha

    (000)

    Grain Yield

    (t/ha)Male Female

    Pool-16 55 59 272.61 94.43 49.26 48.52 4.76Pool-17 E 51 54 200.07 86.07 48.81 49.33 4.47

    Arun-4 53 56 208.45 111.16 48.81 49.63 5.01

    Arun-2 (std.chk) 55 58 209.6 162.77 49.26 48.59 5.31

    Farmers Local 58 61 244.45 132.85 48.07 47.33 4.47

    Grand mean 56 58 227.03 117.45 48.84 48.68 4.8

    F-test ** ns ns ns ns ns **

    CV % 6.09 6.18 61 72.3 7.04 8.54 14.85

    LSD (0.05) 2.41 2.6 101.29 62.11 2.51 3.05 0.52

    (Source: NMRP 2005. Annual Report 2004/05. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 6: Grain yield and attributes of early maize genotypes in CVTE at Rampur, 2006 summer

    GenotypesDays to 50% Pl

    ht

    (cm)

    Earht.

    (cm)

    Pl.asp

    (1-5)

    Earasp

    (1-5)

    Huskcover

    (1-5)

    Grainyield

    (kg/ha)Male Fem.

    POP-445C1 46 50 128 70 2.3 2.8 1.8 2250 ePOP-446C1 44 49 132 53 2.7 2.3 1.5 3250 abcd

    S97TEYGHAYB(3) 43 49 122 50 2.3 2.2 3.7 2340 dePOOL-16SR ZAMBIA 50 55 160 80 2.3 1.8 2.5 2560 cdeZ97EWBF2 53 57 153 65 2.5 2.5 1.8 3530 abPool-17 E*Local 45 49 150 65 3.0 2.0 2.3 2700 bcdeEarly Mid Katumani 55 56 160 72 2.2 2.7 2.3 2790 bcdeArun-1EV 53 58 165 68 2.5 1.8 2.0 3440 abc

    Arun-4 50 54 190 100 2.2 1.8 1.7 4030 a

    Arun-1 (std chk) 46 51 182 88 2.2 2.3 1.5 3340 abcArun-2 (std chk) 47 52 192 85 2.0 2.2 1.7 3160 abcdeFarmers Variety 51 54 178 83 2.7 2.2 1.5 3030 bcde

    Grand Mean 49 53 159 74 2.4 2.22 2.0 3030F-test ** ** ** * **CV% 1.47 3.94 12.92 21.7 15.97

    LSD (0.05) 1.2 3.51 34.86 27 820

    Notes: CV = Coefficient of variation, F-test=Fishers test, LSD = Least significant difference of means, ** = significancelevel ( P

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    Arun-4 51 55 205.7 106.9 2.2 2.2 50 51.4 4780a

    Arun-1(std. Chk) 53 56 230.1 115.94 2.3 2.1 47.9 49.4 4790a

    Arun-2(Std.Chk) 54 56 220.6 116.04 1.2 1 53.7 54.3 4770a

    Farmers Local 57 59 256.3 148.72 2.3 2.5 47.7 50.2 4590a

    Grand Mean 52 55 216.3 112.12 2.25 2.23 48.8 49.93 4.52

    F test ** ** ** ** ns ns **

    CV% 6.73 6.56 9.15 15.66 12.57 13.29 17.09

    LSD (0.05) 3.14 3.25 17.7 15.75 5.5 5.95 690

    (Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)Annex 8: Performance of early maize genotypes FFT Shukranagar-2007

    SN Genotypes No of plants/ha

    (in 000')

    No of ears/ha

    (in 000)

    Grain yield

    kg/ha

    1 Pool-17 E 45778 50667 2987.72

    2 Arun-1EV 44000 41778 3638.67

    3 Z97EWBF2 43556 47111 3765.42

    4 Early Mid-Katumani 45778 48889 3433.92

    5 Arun-1 40444 40000 2953.73

    6 Farmer's variety 47111 44889 3997.03Grand mean 44444 45556 3462.749F-testCV%LSD (0.05)

    ns9.817935

    ns11.129215

    ns13.88874.44

    (Source: NMRP 2008. Annual Report 2007/08. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 9: Performance of early maize genotypes FFT Rajahar-2007

    SN Genotypes No of plants/ha (in 000') No of ears/ha (in 000) Grain yield (kg/ha)

    1 Pool-17 E 45333 43556 3037.44

    2 Arun-1EV 48444 39111 3201.97

    3 Z97EWBF2 50222 45333 3699.46

    4 Early Mid-Katumani 46222 38667 2506.09

    5 Arun-1 45778 40444 2733.21

    6 Farmer's variety 36444 34667 2344.47Grand mean 45407 40296 2920.44F-test

    CV%

    LSD (0.05)

    *

    9.56

    7893

    NS

    12.48

    9148

    NS

    17.22

    914.72

    (Source: NMRP 2008. Annual Report 2007/08. National Maize Research Program, Rampur, Chitwan, Nepal)Annex 10: CFFT Maize (early set) summer, Manahari 2007

    SN Genotypes Plant height (cm) Ear height (cm) Plant/ha

    (No.)

    Yield (t/ha)

    1 Pool-17 E 172 78 45066.67 3.552 Arun-1EV 186 84 42666.67 3.713 Z97EWBF2 180 89 44266.67 3.59

    4 Early mid katumani 182 88 42400.00 3.63

    5 Arun-1 183 94 44266.67 3.906 Farmer's variety 204 107 44533.33 3.50

    F-test ** ** ns ns

    Grand Mean 184.63 90.1 43867 3.649

    CV% 2.75 4.64 4.77 10.68

    LSD (0.05) 2519 0.469

    (Source: NMRP 2008. Annual Report 2007/08. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 11: Adjusted grain yield & its attributes in to early maize genotypes in CVTE at ARS Doti, 2006 summer

    GenotypesTasseling

    (50%)

    Silking

    (50%)

    Pl

    ht(cm)

    Ear

    ht(cm)

    Pl

    asp(1-5)

    Ear

    asp(1-5)

    Plant

    /ha(000)

    Grain

    yield(kg/ha)

    POP-445C1 53 57 215 90 2 3 53.33 5170

    POP-446C1 51 55 205 93 3 3 52.59 5490

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    S07TEYGHAYB(3) 50 54 169 69 3 3 46.67 4990

    POOL-16 SRZAMBIA 54 59 223 105 2 3 57.78 6280

    Z97EWBF2 55 61 215 107 3 3 45.19 6360

    Pool-17 E x Local 53 56 206 93 2 2 33.33 5540

    Early Mid Katamani 54 59 249 132 2 3 58.52 6460

    Arun-1EV 52 58 231 110 2 3 56.3 5150

    Arun-4 51 56 247 111 2 2 45.93 5630

    Arun-1(Std. Chk.) 52 57 253 132 2 2 50.37 5990

    Arun-2(Std. Chk.) 53 58 257 128 2 2 58.52 5840

    Farmers Variety (LocalChk.)

    42 49 197 102 3 3 39.26 3370

    Grand Mean 52 57 222 106 2.33 2.53 49.82 55.21

    F-Test ** ** ** ** ns ** ** *

    CV % 2.87 2.45 8.34 16.3 20 12.73 14.77 15.28

    LSD (0.05) 2.51 2.36 31 29.27 0.55 12.46 1544

    (Source: NMRP 2007 Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 12: Response of early maize varieties to different plant population in winter season 2006/007

    SN TreatmentsDays to 50%

    Lodgingpl/m2

    Plantheight

    (cm)

    Leaf/plant

    (no.)

    Plant/m2

    Cobs/m2

    Barrenplant

    /m2

    Grainyield

    (kg/ha)

    Yield(kg/ha)

    HITasselin Silking

    A Maize Variety1 Arun-4 43.1 51 2.9 180.0 8.25 6.61 6.25 0.37 4793 3200 0.602 Arun-1 EV 44.0 51 3.4 189.0 8.23 6.48 6.16 0.34 4860 3400 0.58

    3 Pool 17 40.4 46 0.9 153.9 7.44 6.86 6.43 0.42 4041 2450 0.62B Plant Spacing1 75 x 25 cm 42.4 49.5 1.7 181.5 8.0 5.21 4.96 0.28 4145 2550 0.622 75 x 20 cm 42.6 49.4 2.2 171.5 7.9 6.34 4.89 0.44 4563 2950 0.67

    3 75 x 16 cm 42.5 49.2 2.9 170.2 7.9 7.6 7.11 0.48 4513 3410 0.57

    4 60 x 20 cm 42.3 49.2 2.8 173.9 8.0 7.45 7.16 0.31 5038 3180 0.61CV% 0.99 0.58 45.3 5.3 5.1 5.62 6.9 55.1 10.58 13.59 4.01LSD (0.05) A 0.68 0.44 1.00 14.70 ns ns ns ns ns 600 nsB ns ns ns ns ns 0.35 0.43 ns 478.3 0.41 0.024

    AB ns 0.49 ns ns ns ns ns ns 828.5 ns ns

    (Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 13: The mean performance of 7 maize varieties to processing methods

    SN TreatmentGrit %

    Flour%

    Bran%

    Grit :FlourRatio

    Hardnessto work (1-

    5)VeryCoar.

    Coar. Med. Fine Total

    A. Grinding Method

    1. Power mill grinding 0 5.6 7.1 48.3 61.1 31.7 7.2 2.0:1

    2.Hand Stone (Janto)

    Grinding

    11.1 22.9 12.1 32.7 78.8 15.2 6.7 5.5:1

    B. Varieties

    1. Manakamana-1 4.5 14.8 9.7 40.6 69.6 23.3 6.8 3.9:1 2

    2. Manakamana-3 4.6 14.2 10.2 40.3 69.3 23.6 7.0 3.6:1 2

    3. Rampur Composite 7.6 14.8 8.6 41.1 72.1 21.2 6.8 4.4:1 4

    4. Arun 4 5.8 14.7 9.2 39.1 68.8 24.5 6.6 3.6:1 3

    5. Upahar 7.8 15.1 10.0 38.4 71.3 25.3 7.6 3.3:1 5

    6. Pop 45 C 10 3.8 12.7 9.6 43.2 69.3 21.6 7.1 4.2:1 3

    7. Arun 1-EV 4.7 13.5 10.1 40.7 69.0 24.5 6.5 3.1:1 1

    CV % 7.2 20.1 15.2 33.0

    LSD (0.05) A 3.2 2.99 ns 0.78

    B ns ns ns ns

    A B ns ns ns ns(Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

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    Annex 14: The mean performance of different maize varieties used to popping quality in earthen pot

    (Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 15: Result of CFFT early set tested at Pakhribas during 2009 Summer season

    SN Genotypes

    GY

    kg/ha

    Height in cm Disease scoring (1-5) HC

    (1-5)

    Aspect (1-5)

    Plant Ear GLS TLB Plant Ear

    1 Pool-17 E 4776 179 133 1 2 2 2 2

    2 Arun-1EV 4714 192 138 1.1 2.3 1.8 1.9 1.8

    3 Arun-4 4275 183 129 1.1 2.5 2.1 1.8 1.8

    4 Arun-2 5114 196 140 1 2.1 1.8 1.4 1.5

    5 Farmer's local 4461 206 145 1.3 2.6 1.1 2.4 2.4

    Grand mean 4668 191 137 1.1 2.3 1.8 1.9 1.9

    F-test ns ** ns ns ns * ns *

    CV% 13.7 2.9 7.6 19 14.5 19.7 24.7 15.8

    LSD (0.05) 983 8.6 16 0.32 0.51 0.53 0.71 0.46(Source: NMRP 2010. Annual Report 2009/10. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 16: Performance of early CVT set combined over DaiIekh, Lumle and Pakhribas, 2005 summer.

    EN Genotypes Days to 50% Plant ht

    cm.

    Ear ht

    cm.

    Plant/ha

    (000)

    Ears/ha

    (000)

    Grain Yield

    (t/ha)Tass. Silk

    1 Pop-445c1 58 62 165 69 49.38 48.40 4.04 a-d

    2 Pop-446c1 58 61 161 77 53.46 51.48 3.58 cd

    3 S97TEYGH"AYB"(3) 59 61 154 75 52.10 51.11 3.27 d

    4 POOL-16 SR zambia 60 64 167 88 39.88 41.36 3.93 a-d5 Z97 EWBF2 60 63 178 93 51.60 50.62 4.67 ab

    6 RAMS03E02 67 71 180 91 48.89 46.42 4.77 a

    7 Early Mid Katumani 60 64 187 105 53.58 51.11 4.23 a-d

    8 Arun-1EV 60 63 187 103 53.70 50.86 4.88 a

    9 Arun-4 59 62 190 92 48.02 47.28 4.07 a-d

    10 Arun-1 (std. chk.) 60 64 186 101 54.44 51.36 4.31 a-c

    11 Arun 2 (std. chk.) 59 62 184 100 53.09 50.25 4.31 a-c12 Farmers Variety 62 66 209 118 53.46 45.68 3.64 b-d

    GM 60.14 63.53 180 92.8 50.97 48.83 4.4

    F-test (G) ** ** ** ** ** NS **

    ( E) ** ** NS NS * ** NS

    (G x E) ** ** ** ** NS NS NS

    CV% 4.43 4.66 10.43 14.94 15.09 16.79 22.39

    SE (G) 0.8 0.99 6.22 4.62 2.56 2.73 0.315

    (E) 0.989 1.1 8.98 6.87 2.24 2.65 0.83(G x E) 1.54 1.71 10.78 8.01 4.44 4.73 0.541

    (Source: NMRP .2006 Annual Report 2005/06. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 17: Mean Grain Yield and Silking in CFFTE Across Location, 2005, Summer

    Varieties Popped grain % Volume increased (by times)

    Manakamana 1 49.2 3.78

    Manakamana 3 39.2 3.23

    Rampur Composite 29.1 2.56

    Arun 4 21.3 2.16

    Upahar 22.2 2.29

    Population 45 C 10 8.6 1.78

    Arun 1-EV 13.6 1.91

    Pop Corn 82.6 12.15

    Grand Mean 33.2 -

    CV % 12.4 -

    LSD (0.05) 7.2 -

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    SN

    Genotypes Days to 50 Silking Grain Yield t/ha

    Dailekh Lumle Pakh. Mean Dailekh Lumle Pakh. Mean

    1 Pool-16 54 66 57 59 3.84 3.67 2.69 3.55

    2 Pool-17 E 48 62 51 53 4.50 2.81 3.97 3.78

    3 Arun-1 EV 55 67 53 58 5.19 3.59 5.40 4.79

    4 Arun-4 56 64 55 59 4.54 3.71 4.63 4.28

    5 Arun-2 (std chk.) 54 66 56 59 4.95 3.18 5.66 4.72

    6 Farmers Variety 50 64 62 59 4.73 3.77 4.31 4.25

    GM 53 64 56 58 4.58 3.29 4.44 4.19

    F-test ns * ** * ns **

    CV% 8.56 2.94 1.11 14.5 20.22 13.9

    LSD (0.05) 2.85 0.82 0.87 0.814

    (Source: NMRP 2006. Annual Report 2005/06. National Maize Research Program, Rampur, Chitwan, Nepal)Annex 18: Performance of CFFT-E05 combined over Dailekh, Lumle, and Pakhribas, 2005 summer.

    EN

    Genotypes Days to 50% Pl htcm

    E htcm

    Pl/ha(000)

    E/ha(000)

    H cov(1-5)

    P asp(1-5)

    E asp(1-5)

    tur(1-5)

    GrainYield(t/ha)

    Tass. Silk

    1 Pool-16 56 59 186.0 85.9 41.48 31.30 2.0 1.6 2.1 1.0 3.55 d2 Pool-17 E 52 53 171.4 76.3 45.83 40.12 2.8 1.5 2.1 1.1 3.78 cd

    3 Arun-1EV 56 58 202.0 114.2 48.89 41.11 1.4 1.8 1.6 1.0 4.79 a

    4 Arun-4 55 59 205.3 106.4 49.29 41.05 1.0 1.6 1.6 1.5 4.28 ab

    5 Arun-2 (std chk) 56 59 212.3 114.3 47.13 40.65 1.3 1.6 1.6 1.0 4.72 ab

    6 Farmers Variety(L.chk)

    56 59 241.7 141.7 47.93 39.88 1.7 1.7 1.8 1.0 4.25 bc

    GM 55.26 57.93 203.0 106.5 46.76 39.01 1.55 1.69 1.81 1.07 4.19

    F-test (G) ** ** ** ** ** ** **

    ( E) ** ** ** ** NS ** **

    (G x E) ** ** ** ** ** ** **

    CV% 4.4 5.22 6.05 9.71 10.38 11.41 14.85

    SE (G) 0.71 0.87 3.55 2.98 1.40 1.29 0.18(E) 1.72 1.73 7.84 5.54 2.31 2.52 .243

    (G x E) 1.23 1.51 6.15 5.17 2.43 2.29 .32

    (Source: NMRP 2006. Annual Report 2005/06. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 19: Grain yield and yield attributing characteristics of early maize genotypes in CVTE (combined over Dailekh,Pakhribas, Lumle), 2006 summer.

    GenotypesDays to 50% Plant

    Height

    (cm)

    EarHeight

    (cm)

    Plants/ha.

    (000)

    Ears/ha

    (000)

    Grainyield

    (kg/ha)Male Female

    POP-445C1 52 54 192.0 95.1 56.17 58.02 4370

    POP-446C1 53 56 187.8 92.1 55.93 61.98 4462

    S97TEYGHAYB(3) 54 56 184.9 107.3 57.90 59.88 4180

    POOL-16 SRZAMBIA 56 58 190.5 100.0 55.43 55.06 4600

    Z97EWBF2 56 60 204.9 108.8 55.68 56.42 4730

    Pool-17 E Local 54 56 194.8 107.5 50.86 55.19 3960

    Early Mid Atumani 56 59 208.5 108.4 56.05 52.72 4200

    Arun-1EV 56 58 198.6 107.8 52.59 52.84 4160

    Arun-4 55 58 198.3 103.6 57.53 59.01 4850

    Arun- 1 (std chk) 56 59 201.5 107.9 53.70 52.59 4090

    Arun-2 (std chk) 56 59 219.8 111.5 57.53 56.79 4480

    Famers Variety 56 59 221.8 118.8 53.95 54.94 4100

    Grand Mean 55 58 200.0 106.0 55.28 56.29 4350

    F-test

    Location (L) ** ** ** ** ** ** ns

    Treatment (T) ** ** ns ns * * ns

    L T ** * ns ns ns ns ns

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    CV% 3.3 3.35 13.87 18.64 7.52 10.67 21.06

    SE

    Location(L) 0.85 0.88 2.83 3.02 1.6 1.37 212.78

    Treatment (T) 0.61 0.64 9.26 6.57 1.4 2 305.2

    L T 1.05 1.12 16.03 11.38 2.4 3.47 528.62

    (Source: NMRP 2007. Annual Report 2006/07. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 20: Combined analysis for grain yield and agronomic traits of maize genotypes under heat stress screening trialsconducted at three different locations (Rampur, Nepalgunj and Parwanipur) in 2010 summer

    SN Genotypes Days to 50 % ASI(days)

    Plantheight(cm)

    Earht.(cm)

    EarLen.(cm)

    Eardia.(cm)

    Pl.Asp.(1-5)

    EarAsp.(1-5)

    GrainYield(kgha-1)

    tasseling silking

    1 RML-7 59 64 4 188 89 11 4 3 4 2719

    2 RL-84 56 59 3 166 79 13 3 3 4 3675

    3 RL-30-1 57 61 3 165 89 14 4 3 4 2890

    4 PUTU-13 61 67 3 139 66 11 3 4 4 2671

    5 Pool-17 E 45 47 3 161 70 11 3 3 4 3221

    6 Arun-1EV 47 50 3 167 79 13 4 2 3 2943

    7 Arun-4 57 62 3 171 75 15 4 2 3 3564

    8 Pool -16 51 55 3 165 78 13 4 3 3 2927

    9 Narayani 50 52 2 170 86 14 4 2 2 4110

    10 Upahar 61 62 5 190 90 14 4 2 2 5228

    11 Across-9331 53 56 2 173 90 14 4 2 2 3888

    12 S 97 TLYHGA YB(3)

    52 54 2 173 77 12 4 2 3 3069

    13 Rampur composite 55 57 3 186 88 13 4 2 3 4086

    14 Manakamana-4 56 57 4 182 87 14 4 3 3 5299

    15 Arun-2 49 53 1 173 85 13 4 2 3 3409

    16 OEHPW 58 61 2 203 97 15 4 2 2 4451

    17 P501SRCO/P 502

    SRCO

    60 64 3 172 69 13 4 2 3 3419

    18 BGBY POP 60 65 6 184 93 16 4 2 2 5110

    19 S99TYQ-HGB 56 58 1 175 84 13 4 2 2 4194

    20 Posilo Makai-1 59 64 4 187 90 15 4 2 2 4636

    21 SO 1 SIWQ3 64 66 3 164 67 13 4 3 3 349822 S 99 TLYQ-B 61 66 2 177 78 13 4 2 3 3419

    23 Rampur SO 3 FO2 56 59 3 190 96 15 4 2 2 4345

    24 Rampur SO 3 FO4 56 59 3 169 78 14 4 2 3 398225 Rampur SO3 FO6 57 60 4 192 92 14 4 2 2 4069

    26 Rampur SO3 FQ-02 62 65 4 183 82 14 4 2 3 3910

    27 BLSB SO7 F 10 54 56 5 174 87 14 4 2 2 410428 BLSB SO 7 F12 57 60 3 183 89 14 4 2 2 446629 TLBRS SO 7 F 14 55 58 2 190 87 14 4 1 2 4727

    30 TLBRS SO 7 F16 56 60 1 188 102 14 4 1 2 514431 R POP-1 55 57 2 191 93 13 4 1 2 441132 R POP-2 50 53 2 196 101 13 4 2 3 445333 R POP-3 53 56 4 178 93 14 4 2 3 419434 R POP-4 51 53 3 191 99 14 4 1 2 4799

    35 NML-1/NML-2 56 57 1 187 97 15 4 2 3 4780

    36 RML-4/NML-2 64 67 2 166 78 14 4 3 3 3555

    37 RML-8/RC 49 51 2 184 86 14 6 3 3 495838 Manakamana-3 58 60 4 191 98 15 4 2 2 4206

    39 Rampur2 51 53 3 171 76 14 4 3 2 337540 Deuti 57 60 5 192 98 16 4 1 3 4185

    41 Pop-446 50 53 3 154 62 12 4 3 4 1883

    42 Khumal Yellow 51 55 4 177 86 13 4 2 2 4366

    43 Gulmi-2 54 56 2 234 122 15 4 2 3 373444 Resunga Composite 54 57 4 200 94 14 4 2 2 4631

    Grand mean 55 58 3 180 86 14 4 2 3 3971

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    CV (%) 5.40 5.70 44.02 9.56 14.05 13.32 20.7 42.86 27.86 28.70SD 4.39 4.83 1.12 15.29 11.25 1.19 0.40 0.61 0.66 771

    LSD (0.05) 3.36 3.74 1.52 20.63 14.33 2.12 0.93 1.06 0.83 1299

    F-test Genotypes (G) ** ** ** ** ** ** ns ** ** **

    Location (L) ** ** * ns * * ns ns * **

    Interaction (G L) ** ** ns NA NA * NA * ** ns

    ASI: anthesis-silking interval, pl:plant, ht: height, Len.: length,dia: diameter,Asp.:aspect and Gr.:grain, NS=Nonsignificant, NA:Not available, * significant at 1% level and **significant at 5% level. (Source: NMRP 2010. AnnualReport 2009/10. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 22: Combined analysis on pest infestation of maize genotypes under heat stress screening trials conducted at threedifferent locations (Rampur, Nepalgunj and Parwanipur) in 2010 summer

    SN Genotypes Wilt % Stem borer%

    S N Genotypes Wilt % Stemborer %

    1 RML-7 20.17 3.83 26 Rampur SO 3 FQ-02

    2.33 0.0

    2 RL-84 6.0 0.00 27 BLSB SO7 F 10 3.17 1.50

    3 RL-30-1 1.17 5.0 28 BLSB SO 7 F12 6.0 0.834 PUTU-13 11.0 2.17 29 TLBRS SO 7 F 14 2.0 0.05 Pool-17 E 12.83 8.33 30 TLBRS SO 7 F 16 7.17 0.0

    6 Arun-1EV 6.17 1.67 31 R POP-1 8.33 0.07 Arun-4 2.0 3.17 32 R POP-2 9.0 0.08 POOl -16 9.0 2.67 33 R POP-3 18.33 1.17

    9 Narayani 3.83 0.83 34 R POP-4 8.33 0.8310 Upahar 1.67 6.83 35 NML-1/NML-2 14.50 2.5011 Across-9331 2.50 0.0 36 RML-4/NML-2 4.17 8.012 S 97 TLYHGA

    YB (3)7.0 1.83 37 RML-8/RC 3.0 0.0

    13 RC 8.50 0.0 38 Manakamana-3 7.0 0.014 Manakamana-4 1.50 7.17 39 Rampur-2 4.0 2.17

    15 Arun-2 6.33 1.17 40 Deuti 4.67 0.016 OEHPW 7.0 0.72 41 Pop-446 10.33 0.017 P 501SRCO/P

    502 SRCO8.83 5.83 42 Khumal Yellow 6.33 0.0

    18 BGBY POP 6.00 0.83 43 Gulmi-2 1.50 2.0

    19 S 99 TYQ-

    HGB

    1.83 2.0 44 Resunga

    Composite

    11.17 0.0

    20 Posilo Makai-1 5.50 4.0 Grand mean 7.11 1.94

    21 SO 1 SIWQ-3 11.17 0.0 CV (%) 131.67 266.6422 S 99 TLYQ-B 19.0 0.83 SD 4.687 2.31323 Rampur SO 3

    FO26.33 2.33 LSD (0.05) 10.89 5.827

    24 Rampur SO 3FO4

    10.83 2.33 F-test Genotypes(G)

    * ns

    25 Rampur SO 3

    FO6

    5.17 2.67 Location (L) NA ns

    Interaction (G L) NA NA

    NS=Non significant, NA: Not available, * significant at 1% level(Source: NMRP 2010. Annual Report 2009/10. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 24 : Performance of Early Set maize genotypes in CFFT at Pakhribas in 2010 summer

    SN GenotypeDisease scoring(1- 5) Height Flowering

    Yield (kg

    ha-1)E.tur H.maydis plant ear male female

    1 POOL-17 E 1.625 2.125 182.5 143.3 54.75 59 2992

    2 ARUN-1EV 1.625 2.25 185.8 142.5 62.25 66.75 3501

    3 Arun-4 1.625 2.25 177 140.3 62.5 66.5 3265

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    4 Arun-2 1.625 2.375 186.8 147 60.75 65 4106

    5 FARMERS'VARIETY

    2.625 3.125 265 179.3 62.5 68 3043

    Grand mean 1.825 2.425 199.4 150.5 60.55 65.05 3381

    CV% 14.1 12.1 5.7 3.6 1.7 1.4 19.5

    LSD(0.05) 0.3978 0.4503 17.62 8.36 1.622 1.434 1016.1

    F-test ** ** ** ** ** ** NS

    (Source: NMRP 2011. Annual Report 2010/11. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 25 : Performance of Early Set maize genotypes in CFFT at Chambas in 2010 summer

    SN GenotypeHeight Flowering

    Yield (kgha-1)plant ear male female

    1 POOL-17 E 175.4 82.4 51.6 53.6 2977

    2 ARUN-1EV 195 89 53.8 55.8 4785

    3 Arun-4 215.4 108 55.6 57.6 5003

    4 Arun-2 216.6 100.6 56 58 4608

    5 FARMERS' VARIETY 236 116.4 60.2 62.2 4446

    Grand mean 207.7 99.3 55.44 57.44 4364

    CV % 7.4 15.1 1.9 1.8 9.6

    LSD (0.05) 20.51 20.09 1.416 1.416 559.3

    F-test ** * ** ** **

    (Source: NMRP 2011. Annual Report 2010/11. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 26: Maize FFT Early set at OR site Rajahar 2062/063 (2005/06)

    Treat. Genotypes Plant Ht.

    Cm

    Ear Ht.

    Cm

    # Plants/

    7.5 m2

    # Ears/

    7.5 m2

    Grain Yield

    t/ha

    % Yield difference

    over FV

    1 Pool-16 149 72 20 20 2.374 -16.23

    2 Pool-17 E 146 59 34 31 3.237 14.22

    3 Arun-1 EV 152 79 33 30 4.019 41.81

    4 Arun-4 143 73 30 29 3.572 26.04

    5 Arun-2 138 68 31 26 3.455 21.91

    6 Farmers Variety 152 65 25 25 2.834

    GM 146 69.11 28.78 26.78 3.248

    F-test ns ns ** ** *

    CV% 11.86 19.75 16.5 17.9 23.3

    LSD (0.05) 20.65 16.23 5.65 5.7 0.900

    (Source: NMRP 2006. Annual Report 2005/06. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 27: The mean performance of early maturing maize varieties for grain yield and other traits in on-farm evaluation

    trials conducted in Syangya and Palpa, 2001

    SN Varieties Days totasseling

    Days tosilking

    Plantheight(cm)

    Ear height(cm)

    No. ofplants/9m2

    No ofears/9m2

    G rainyield(kg/ha)

    1 Pool-15 39 44 179 109 40 40 3190

    2 Pool-17 E 47 52 197 114 40 40 3340

    3 Arun-2 46 52 218 112 41 41 4090

    4 Arun-1 47 52 219 115 39 40 3850

    5 Arun-4 47 52 207 125 41 41 4420

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    SN Genotypes Grain

    yield

    Tassel

    ing

    Silk

    ing

    ASI Plant

    height

    Ear

    height

    Plant

    stands/ha

    No.

    ofears/ha

    Prolifi

    cacy

    Days

    tomatu

    rity

    Husk

    cover(1-5)

    1 Pool 17E 4503 B 56 59 3 209 93 50555 57592 1.14 99 3.5

    2 Pool 15E 3676 B 54 57 3 207 96 48333 54444 1.13 99 2.3

    3 Arun-4 5797 A 57 61 4 238 126 51111 55555 1.09 104 1.5

    4 Pool 16 5799 A 57 61 4 233 117 52222 55370 1.06 104 2.8

    5 Arun-2 5748 A 58 63 5 236 127 52778 55555 1.05 105 1.8

    6Farmersvariety 5554 A 57 62 5 278 153 53333 54259 1.02 105 1.5

    Grand

    mean 5229 56.25 60.5 4 233 118 51389 55463 1.08 103 2.3

    P-value

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    Annex 31: Income from maize and soybean intercropping, winter 2008, NMRP Rampur

    S.N. TreatmentsSoybean

    yield

    (kg/ha)

    Maizeyield

    (kg/ha)

    Net income fromsoybean (NRs/ha)

    Net incomefrom maize

    (NRs/ha)

    Total netincome

    (NRs/ha)

    1 Shitala 878 7415 11353 61222 72575

    2 Arun 2 725 5677 6752 35149 419013 Arun 4 844 6215 10307 43228 53535

    4 Manakamana 3 683 6462 5497 46933 52430

    5 Arun 1EV 809 5776 9261 36640 45901

    6 Deuti 913 6224 12399 43364 55763

    7 Pool 17 E 882 4201 11458 13008 24466

    8 Manakamana 4 760 8091 7797 71370 79167

    9 QHG-AB 718 7093 6542 56401 62943

    10 S99TLYQB 781 6768 8425 51518 59943

    11 Rampur Composite 620 7422 3614 61325 64939

    12 Across 9944 xAcross 9942

    851 7631 10516 64469 74985

    Grand mean789 6581 8660 48719 53379

    P-valuens * ns * *

    LSD (0.05)259 1915 7765 28722 29395

    (Source: NMRP 2009. Annual Report 08/09. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 32: Combined data of disease and insect scoring in Arun-1EV, Arun-4 and Pool-17 E over improved variety as

    check (Arun-2) at Sukranagar, Rajahar and Manahari in 2010 summer

    Aspects Arun-4 Arun-1EV Pool-17 E Arun-2

    1. DiseaseTurcicum leaf blight (TLB), 1-5 2.0 1.7 1.6 1.6Grey leaf spot (GLS), 1-5 1.1 1.1 1 1

    Helminthosporium leaf blight 2.25 2.25 2.12 2.37

    Annex 30: Summary sheet of yield attributing traits recorded in early maize CVT in 2004, 2005 and 2006 at Pakhribas

    (Dhankuta)

    SN Varieties Silk (days) Ear height(cm

    Plantheight(cm)

    Root lodgingplt/ha

    Shootlodgingplt/ha

    BarrenPl/ha

    1 S97TEYGHAYB(3)

    66 93 181.5 1235 3210 1975

    2 Arun-4 68 98 198.6 2593 1728 3457

    3 Pool-16 SRZAMBIA

    68 93 183.5 1481 864 2963

    4 Z97EWBF2 70 93 191.7 988 1358 3086

    5 Arun-1 EV 70 102 198.8 741 1975 3827

    6 Arun-2 69 107 204.1 1235 2716 3580

    7 Local check 73 146 246.4 2469 4321 6914

    Grand mean 69 104 200.7 1534 2310 3686

    F pr

    Entry ** ** ** ns * **

    year ** ** ** ns ** **

    Entry x year ** ** * ** ** **LSD (0.05)Entry 1.88 17.5 19.11 1560 1901 2133

    year 1.23 11.4 12.51 1021 1244 1397

    Entry x year 3.26 30.3 33.09 2702 3292 3695

    CV% 2.9 17.6 10.0 107 86 60.7

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    (HLB), 1-5

    Wilt (%) 2 6.17 12.83 6.33

    2. InsectStem borer (%) 3.17 1.67 8.33 1.17

    (Source: NMRP 2011. Annual Report 2010/11. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 33: Major identifying characteristics of Arun-4, Arun 1EV and Pool-17 E at NMRP Rampur in 2010 summer

    (Source: NMRP 2011. Annual Report 2010/11. National Maize Research Program, Rampur, Chitwan, Nepal)

    Annex 34.Performance of different early season maize genotypes tested in CFFT Dailekh, 2011

    SN Genotypes Tasseling

    days

    Silking days Plant height (cm) Ear height

    (cm)

    Grain

    Yield(kg/ha)

    1 Pool-17 E 57.33 59.33 233.7 122 4421

    2 Arun-1EV 59.33 61.33 230.3 120.8 5149

    3 Arun-4 59.33 61.33 239.6 128.8 4651

    4 Arun-2 59.67 61.67 254.7 150.9 4907

    5 Farmer's Variety 67.33 69.33 282.2 156 4812

    Grand mean 60.60 62.60 248.1 135.7 4788

    P-value