MISSIONThe MRC Core’s mission is to provide scientists with access to the latest molecular technologies for exploring basic biological mechanisms and the molecular basis of human disease.

INTRODUCTIONThe Molecular Resource Center (MRC) Core, established in 1985, is a Tennessee Higher Education Commission (THEC) Center of Excellence serving the University of Tennessee Health Science Center (UTHSC) campuses. In order to accomplish its mission, the MRC invests in both state of the art equipment and trained personnel. The MRC houses a full range of equipment that facilitates molecular, genetic, and biochemical studies at the sub-cellular and systems biology levels. In addition to providing technical services, MRC personnel assist and instruct investigators in applying specialized tools of modern molecular biology to basic and clinical research. The MRC’s services include sequencing at both the single fragment and whole genome/transcriptome (next-generation sequencing, NGS) levels along with offering Affymetrix microarrays. Equipment, reagents, and training for real-time PCR and automated isolation of nucleic acids are also provided.

SERVICES• Nucleic Acid (DNA/RNA) Isolation (Qiagen QIAcube)• Nucleic Acid Quality Analysis (Quantification and

quality determination)• Sanger Sequencing (Plasmids, fragments & PCR

products) • Next-generation Sequencing (ChIP, targeted or

whole genome or exome, & microbiome)• Gene Expression Analysis (Affymetrix microarrays,

total RNA and miRNA)• Gene Expression Analysis (RNA-Seq)• Gene Expression Analysis (qRT-PCR & digital PCR)• Genotyping (Capillary Electrophoresis for STR & SNP

analyses)• Genotyping (qPCR & copy number analysis by digital

PCR)• Genotyping (Next-generation sequencing- targeted

& exomes)

• Methylation Analysis (Sanger sequencing)

• Equipment for Client Use -Robotic Liquid Handler,

Multimode Plate Reader, Fluorescence Microscope

Molecular Resource Center of Excellence (MRC)A University of Tennessee Health Science Center Institutional Core

CORE DIRECTOR: WILLIAM TAYLOR, PHD

EMAIL: wtaylor@uthsc.edu

Translational Sciences Research Building (TSRB), 71 S. Manassas, Suite 110

(901) 448-6165

EXECUTIVE DIRECTOR: TIFFANY SEAGROVES, PHD

tseagro1@uthsc.edu(901) 448-5018

CORE INFORMATION

Introduction The Molecular Resource Center (MRC) Core, established in 1985, is a Tennessee Higher Education Commission (THEC) Center of Excellence at The University of Tennessee Health Science Center (UTHSC). In order to accomplish its mission, the MRC invests in both state of the art equipment and trained personnel. The MRC houses a full range of equipment that facilitates molecular, genetic, and biochemical studies at the sub-cellular and systems biology levels. In addition to providing technical services, MRC personnel assist and instruct investigators in applying specialized tools of modern molecular biology to basic and clinical research. The MRC’s services include sequencing at both the single fragment and whole genome/transcriptome (next generation sequencing) levels along with offering Affymetrix and Illumina microarrays. Equipment, reagents, and training for real-time PCR and automated isolation of nucleic acids are also provided.

Services• Nucleic acid (DNA/RNA) isolation (Qiagen QIAcube)• Nucleic acid Quality analysis (quantification and quality determination)• Sanger sequencing (Plasmids, Fragments & PCR products)• Next-gen sequencing (ChIP, targeted, Genome, Exome, & Microbiome; )• Gene Expression Analysis (Affymetrix microarrays, total RNA and miRNA)• Gene Expression Analysis (RNA-Seq)• Gene Expression Analysis (qRT-PCR & digital PCR)• Genotyping (Capillary Electrophoresis for STR & SNP analyses)• Genotyping (Illumina Microarrays)• Genotyping (qPCR & copy number analysis by digital PCR)• Genotyping (Next Gen sequencing- targeted & exomes) • Methylation analysis (Illumina microarrays & Sanger sequencing)• Equipment for client use -Robotic Liquid Handler, Multimode Plate Reader, Fluorescence Microscope

Equipment and Software• Qiacube Robot (Qiagen RNA/DNA isolation) • Agilent Bioanalyzers (2) • Nanodrop 1000 and 8000 Spectrophotometers • Applied Biosystems 3130XL Sequencer • Ion Torrent PGM Sequencer • Ion Torrent Proton Sequencers (2) • Illumina NextSeq 500 • Affymetrix GeneChip System • Illumina iScan System • Roche LightCycler 480 Systems (2) • Fluidigm Biomark • Eppendorf epMotion 5075 Liquid Handling Robot • Spectramax m2e multimode plate reader • Zeiss Axiophot Microscope • Partek Flow and Genomics Suite Software • Ingenuity Pathway Analysis (IPA) Software

The MRC Core’s mission is to provide scientists with access to the latest molecular technologies for exploring basic biological mechanisms and the molecular basis of human disease.

Molecular Resource Center of Excellence (MRC)

UTHSC Research Cores and Shared Resources

UTHSC Institutional Cores are dedicated to the success of your

project. We serve the UTHSC research community by providing access to

state-of-the-art equipment and to expert consultation services.

http://www.uthsc.edu/research/institutional-cores/index.php

CORE STAFF

Assistant Director: Tom Cunninghamtcunningham@uthsc.edu

(901) 448-6191

Research Associate: Jian Yanjyan1@uthsc.edu

(901) 448-6042

Research Specialists: Lorne Roselrose4@uthsc.edu

(901) 448-8229

Felicia Waller fwaller@uthsc.edu

(901) 448-8746

Caitlin Costelleccostel2@uthsc.edu

(901) 448-6191

The University of Tennessee is an EEO/AA/Title VI/Section 504/ADA/ADEA institution in the provision of its education and employment programs and services.

For more information:

Molecular Resource Center of Excellence (MRC)71 S. Manassas, Memphis, TN 38163 | 901.448.6165uthsc.edu/research/institutional-cores/mrc/

The University of Tennessee is an EEO/AA/Title VI/Title IX/Section 504/ADA/ADEA institution in

the provision of its education and employment programs and services.

EQUIPMENT AND SOFTWARE

• Qiacube Robot (Qiagen RNA/DNA isolation)

• Agilent Bioanalyzers (2)

• Nanodrop 1000 and 8000 Spectrophotometers

• Applied Biosystems 3130XL Sequencer

• Ion Torrent PGM Sequencer

• Ion Torrent Proton Sequencers (2)

• Illumina NextSeq 500

• Affymetrix GeneChip System

• Roche LightCycler 480 Systems (2)

• Fluidigm Biomark

• Eppendorf epMotion 5075 Liquid Handling Robot

• Spectramax m2e multimode plate reader

• Zeiss Axiophot Microscope

• Hamilton STARlet robot for automated NGS library

preparation

STAFFWilliam Taylor, PhD, Core Director71 S. Manassas, Suite 110901.448.6165wtaylor@uthsc.edu

Tiffany Seagroves, PhD, Executive Director71 S. Manassas, Suite 110901.448.5018tseagro1@uthsc.edu

Tom Cunningham, Assistant Director71 S. Manassas, Suite 110901.448.6191tcunningham@uthsc.edu

Lorne Rose, Research Specialist71 S. Manassas, Suite 110901.448.8229lrose4@uthsc.edu

Felicia Waller71 S. Manassas, Suite 110901.448.8746fwaller@uthsc.edu

UTHSC RESEARCH CORES AND SHARED RESOURCES

UTHSC Institutional Cores are dedicated to the success of your project. We serve the UTHSC research community by providing access to state-of-the-art equipment and to expert consultation services.

uthsc.edu/research/institutional-cores/index.php