.-

[ | gg_apr unr urm u m m uur u u m u mm m mmdu m mJlrJlsJ6k E, no = = mmmist_--m m = = =m mm m mm m m m m m m m m ,,

| CC Form 374A U.S. NUCLE A'l CELULATOftY COMMIS$lON |p3ng 3 og 1 -pgggg| NI Uce;.01pmber | ,

L ll I~

-1- MATERIALS LICENSE 34-06903-05 '

y' " " * * " " " '

SUPPLEMENTARY SHEET |030-02764 |

|Amendment No. 66 I

I

University of Cincinnati- fMail Location 569 t234 Goodman Street iCincinnati, OH 45267 L >

L

in accordance with letter dated llay 17, 1990, License llumber 34-06903-05 is amended as ffollows: i>C

: Conditions 11.A., 11.C. and 20. are amended to read: I

I

11'. A. Licensedmaterialshallbeusedk,hrkrdet'/thesupervisionof, individuals I

rdesignated by the- licensee"sFifaMiation SafiltPCdmmittee,JamesL.Lessard,Ph.DyChairman, y [. [

V tThe Radiation Protect) ion Officer for the activities abthorized by this licenseC. I

is Victoria R. Molrris. The Assistant Radiation Safety',0f;ficer for the L

4P Iactivities auth6r:izedsby4his license is Howard R;rElsori,L.h.D.\e a ,% .

v, i WD in.this/ M /1 cense, therl[

[

Except as specificafly providedjot'hgrwise ?'s'athme)ntst represent'jcensee shall20.conduct its progra6'in accordancelwl,th 'the t ations, and L

procedures containoff in the docsinent's inAludinghaWenclosures,Oisted below, i

The fluclear Regulatdry Couaissioy%the' gilla'tibas'slall' govern unless the statements,@re L

li 'Qa Irepresentations arid [procedtifesttit

1 a l lh @T@pplication a& correspondence are.morerestrictivethan'thgrjdgula'ti6nsf.~[censee [;TTN f :' ' 3

M h Lh W 41Application dat_e'd_ Augtnj'!13,JB4 ikt udii)g att ch'ments-dated August 9,1984;i t !A.

qj.ap"('g![%Lg ,C9 {!and g)u q /rgc Lg

Letters dated Apr i,1#11,1986, July /2,W986,,ej;;Tebruary 17,1989, February 27, [1yiB.

1989, April 24,1989frygust 22,1989fAugust 23, 1989 (2 documents), giAugust 31,.1989, llovember 30, 1989, February 22, Q990, and llay 17, 1990. ei'

y| Q tY Yr ), )n:s Y Ce n ' t

[ lg :

L \I ;

t !

E 'll JE

'

t i'E

E :

>For the U.S. Iluclear Regulatory Commission E '

E i

2C3 iE

; Original Signed g

Date: June 14, 1990 By William J. Adam, Ph.D. L P,

9010220250 900614 llaterials Licer. sing Section, Region Ill I'

REC 3 LIC30 h I'

MATLSLICENSING PDR bl: g ,

----- --mm---.-,- , m _ _

Y. _ _ _ . m a

P 7'

p.-

'. ,.

:,4- 1A" .

a

JUN 141990 -

-University of CincinnatiATTN: Ms. Victoria R. Morris

_ Radiation Safety Officer234 Goodman Street '

,

Mail Location 569-Cincinnati, OH= 45267 l

Gentlemen:

Enclosed are Amendment Nos. 66, 07, 05, 08, 06 and 07 to your NRC License #

Nos.g34-06903505-|d34-06903-09,34-06903-11,34-06903-13,S00-265andSNM-490,respeitiiely.

~

Please note, effective June 427, 1988 the NRC established technical and financialcriteria for requiring the submission of plans (53 FR 24018). Specifically,Sections 30.35 " Financial Assurance and Recording for Decommissioning" and30.36 " Expiration and Termination of Licenses" of Title 10 Code of FederalRegulations Part 30 addresses decommissioning planning needs, timing, fundingmethods, and environmental review requirements for public and private facilitiesholding by product material licenses. Based upon review of your licensed

~

material possession limits, it appears that it may be necessary for yourorganization to comply with the-above regulations. The decommissioning fundingplans, . certification of financial assurance, etc., are to be submitted for *

review and approval by the NRC on or before July 27, 1990,

Please review the enclosed document carefully and be sure that you understandall conditions. You must conduct your program involving radioactive-materialsin accordance with the conditions of your NRC. license, representations made inyour: license: application, and NRC regulations. In particular, note that youmust:

,

1. Operate in accordance with NRC regulations 10 CFR Part 19, " Notices,Instructions and Reports to Workers; Inspections," 10 CFR Part 20," Standards for Protection Against Radiation," and other applicableregulations.

2. Possess radioactive material only in the quantity and form indicatedin your license.

s

3. Use radioactive material only for the purpose (s) indicated in yourlicense.

I

4. Notify NRC in writing of any change in mailing address.,

|

|

..

-

g-U

' '

7

,%"o

University of Cincinnati 2-

5. Request and obtain appropriate amendment if you plan-to change ownershipof your organization, change locations of radioactive material, or makeany other changes in your facility or program which are contrary: to your.license conditions or representations made in your license applicationand any supplemental correspondence with NRC. Any amendment requestshould be accompanied by the appropriate fee specified in_10 CFR Part 170.

6. Submit a complete renewal application with proper fee or termination-request at least 30 days before the expiration date on your license.You will receive a reminder notice approximately 90 days before theexpiration date. Possession of radioactive material af ter your license -expires is a violation of NRC regulations.

7. Request termination of your license if you plan to permanentlydiscontinue activities involving radioactive material prior toyour expiration date.

You will be periodically inspected by NRC. Failure to conduct your program inaccordance with NRC regulations, license conditions, and representations in yourlicense application will result in enforcement action against you in accordancewith the General Policy and Procedures for NRC Enforcement Actions, 10 CFRPart 2, Appendix C.

If you have any questions or require clarification of any of the above statedinformation, contact us at (708) 790-5625.

Sincerely,

Original Signed ByWilliam J. Adam, Ph.D.Materials Licensing Section

Enclosures:1. NUREG-13362. NUREG 13373. 10 CFR Part 30

RIIIWAdam /ib06/S/90

_ _ _ - . _____-_. _ _-.

r p' }'

. ..m*

.. ,

! 's : ,

, ;<>J

: (FOR LFMS USE)

.O--: ;INFORMATION-FROM LTS. . -

BE. tween:. : -----------------_--

:'LICENSE ~FEELMANAGEMENT BRANCH, ARM : PROGRAM C00.:~02210-

O,- AND : STATUS CODE: 0REGIONAL' LICENSING-SECTIONS- : FEE CATEGORY: * 3P EX~7C 3P

: EXP. DATE: 19' 0531'~

f

O : FEE COMMENTS: iTO.21cA>c9)_ STATE !'

: : ::::::::::g, n'V : LICENSE FE E TR ANSMI17AL

-

.

A. REGION

'

L -1. -APPLICATION ATTACHED.

LAPPLICANT/ LICENSES:- CINCINNATI, UNIVERSITY OF

.O _ RECEIVE 0 DATE: 900521>

~0OCKET NO: 3002764CONTROL NO.: 389417 4

,-O. license.NO.: 34-06903-05 :.

ACTION TYPE: AMENDMENT

O 2. eeE ATTACse0AMOUNT: ___[.___ dCHECK NO.: __,(f_____. Jw +<t ,

cpp J, *I ;,;A??ba vac0p "4 D"A *#- .

A~

3-,

.g f ,n o W : a r A SIGNED _ 2 _ d a .g ___________"

|O uf %____c u_.r.6._____________ ,OATe -v.a

l. O- B LLICENSE FEE MANAGEMENT BRANCH (CHECK WHEN MILES PO 0 / _/)'.

'-

_k _,t_3__ k )_( _ [_( _ l_y_ W _// g g'

'

' 1. FEE-CATEGORY AND-AMOUNT: . ___ _ ___

.O. CORRECT FEE PAID.

.. .

2. PLICATION MAY BE PROCESStu run. .it*

# 2AMENOMENT _____ __,,_____

.O. RENEWAL .i______________

LICENSE i______________

'.0 : 3. 0THen __________________ ______________

________________________________ , ,

TO SIGNED -

DATe _s__ 3 1 II___________________ _____ _

, _________ __,

.O,

|'

.

'D

.r3''

cV<

..(t-'' J.

.

f' R.- ..

y_ *

c, *, "

<

.j.

_. & ''

-

r University of Cincinnati Office of the~

Medical Center - Senior Vice Presidentand Provost for Health Affairs

i

" -

Cincinnati, Ohio 45267 0663 'I141 Health Professions Building...

'

Phone (513) 558-6052FAX (513) 558-2962

P

i

|

$

< -

i- h 1

mg#

grme

May 17, 1990 $ 0Eb (8 F,y esDJ gy o

Mr. George M. McCann~

Id. f",

'"U.S. Nuclear Regulatory CommissionChief Materials Licensing Section799 Roosevelt RoadGlen Ellyn, IL 60137

i

af[~ ~~"( )['REF: NRC License No. Docket No.

34-06903-05 030-02764 ijf' ' - -q-h,, ,'F-

,,

34-06903-09 030-11331 cye . e--,, .,

34-06903-11 030-18949 _/ gd'.j'' - g<. J34-06903-13 030-20526 }

-

l ,r , g*/M _.e '

SNM-490 070-00539 y. ,%-

f o,' 'b' ' h"

' ''"SUD-490- 070-02678 C'

f - 3'O'- -- - 1't ':SUBJ: LICENSE AMENDNENT REQUESTS

- --- ' Q ,- -. __ , ,',' -

' " ~ -Dear Mr. McCann: ~4~ ~; -;

The University of Cincinnati requests that all NRC licenses referencedabove be amended as follows: i

1. To name Victoria R. Morris, M.S. as the Radiation Safety Officer j

(RS0) effective July 1, 1990.!

Ms. Morris received her Master of Science degree in Health Sciences(with majors in Health Physics and Industrial Hygiene) from Purdue-University; is presently the Radiation Safety Of ficer (RS0) for ,

Merck, Sharp and- Dohme -Research Laboratory and is a Certified !

Health Physicist (possessing American Board of Health Physicscertification), thus meeting the RSO requirement specified in 10

CFR 35.900(a).,

!

2. To name Howard R. Elson, Ph.D. as the Assistant RadiatiorREEEIVED 4

Officer (ARS0) effective July 1, 1990.

MAY 2(f'

1990

REGION IIIPatient Care * Education * Research * Community Service MAY 2J 1990An affirrnative action / equal opportunity institution gg4g7

Q;_ ,.

~

._ .__ ____- ___ _ - _ _ _ --_-______ _-

.. . -.,.

3' '....

Mr. George M. McCannMay 17, 1990Page II

Dr. Elson is presently the Radiation Safety Officer (RS0) for theUniversity of Cincinnati and has been specifically named on allabove-ref'erenced licenses since August 1989. Documentation of histraining and experience was submitted to your office at that time.

3. To name James L. Lessard, Ph.D. as the Chairperson of the RadiationSafety Committee (RSC), effective June 15, 1990.

Dr. Lessard received his Ph.D. in Biochemistry from MarquetteUniversity. He received training and experience in the use ofradioactive material (H-3, C-14 and P-32) at the Roche Institute ofMolecular Biology from 1969 to 1971 and has been an Authorized User '

under the University of Cincinnati's broad-scope NRC license since 4

1974 for H-3, C-14, P-32 and I-125.|

Dr. Lessard is presently an Associate Professor of Pediatrics and,

Assistant Professor of Biological Chemistry at the University of. Cincinnati and has been an active member- of the University'sRadiation Safety Committee since April 1, 1989 (also serving on theCommittee's Radiation . Safety Task Force).t Dr..Lessard is aware of<RSC Chairperson responsibilities and the necessary devotion of timeto this appointment.

In regard to the Radiation Safety Committee Chairperson, JudithHarmony,.Ph.D. vill continue to serve in this capacity until. June15,-1990, when Dr. Lessard vill assume the responsibility (pendingNRC approval). Dr. . Harmony vill remain a voting member of the ;

committee.

The training and experience of Ms. Morris and Dr. Lessard are contained inthe attached curricula vitae. In addition, enclosed find the duties,

!authorities and responsibilities of the Radiation Safety Officer (RS0) andAssistant Radiation Safety Officer (ARS0).

As has been discussed with representatives of NRC Region III management,the University of Cincinnati is actively preparing its broad scope license '

renewal application for early submittal to your office. Your approval ofthe above amendment application is requested at this time so that thenewly appointed individuals may begin applying their expertise towards the '

University's revamped Radiation Safety Program in our continuing concertedeffort to provide a safe and efficient environment for radioactive '

material research and the highest quality of patient care.

|

.. . . , . -4 .. ,,

.a - .. . .,-.s.. ..

LE t .

.- |:)

L, 1

| |11 Mr. George M. McCann ;

May 17, 1990Page III j

|

|

|

. Should .you have' questions or comments in regard to the above, please!. - contact this office. Your time and consideration in this matter are

.

grehtly appreciated..

Sincerel'y,

so mea ,

i

Donald C. Harrison, MWl. :.

'Senior Vice President'andProvost.for Health Affairs

' DCH/cvk/j h

|: Enclosures: As stated '||

1 .cc v/o encli H.R. Elson, Ph.D.J.A. Harmony, Ph.D.J.L. Lessard, Ph.D.V.R. Morris, M.S.

4

Li

| .\ '

i

,

tY- ( I k

'.$

$

cournot No. 8 9 4 l' 7. _ . . _ _

p .. .

. ..

..,

'

.'|| .

.

AUTHORITIES, RESPONSIBILITIES AND. DUTIES OF THE RADIATION SAFETYOFFICER (R80) AND ASSISTANT RADIATION SAFETY OFFICER (ARSO)

i

1. Radiation Safety Officer

,

The RSO derives. authority from the President of the |Universitys of Cincinnati and is the Radiation SafetyCommittee's authorized representative regarding radiationprotection within the confines of UC. While ultimateresponsibility for radiation safety rests with the Univer-sity- President and the Radiation Safety Committee (RSC), i

the RSO is delegated responsibility for day-to-day opera- |tions, including implementation of the RCSP, and for !providing such services as may be required by the program. ''

The RSO's duties and responsibilities are as follows:

[ ~ to regularly communicate with the Chairperson of theL RSC concerning RCSP matters

- to present reports of activities at meetings of theRSC

- to.1 provide for a personnel radiation monitoringprogram'

;

- to establish and implement a radiation safety :

training program 1|

- to perform or supervise periodio surveys of all RAM,

and RPD use facilities '

!- to exercise surveillance over the procurement of all !

RAM and RPD'

'

- to inspect facilities and equipment to be used in jL conjunction with RAM and/or RPD '

- to provide consulting services to personnel at allu'

levels of responsibility on all aspects of radiation '

by protection

p - to supervise and coordinate a RAM and RPD disposalp program

I

4

k:. . .

g . , -,-- -- - -

,,

*-; s - . *,

'Il~ - . ~ -2-,

l-

- to perform leak tests of all scaled RAM sources (as ;applicable)

- to conduct physical inventories of all sealed RAMsources

- to maintain current copies of all applicable rulesand regulations for control of RAM and RPD

- to inform AUs of changes in rules and regulations-3

|- to maintain all records associated with the !administration of this program

!

- to review and approve (with the RSC) applications for.use of RAM or RPD

- to provide surveillance over all outgoing shipments of> RAM

- to maintain inventory control of all radiation sourcest

~

- to notify female radiation workers of the risks ofi !prenatal exposure (currently using NRC RegulatoryGuide 8.13)

,,

- to foster the ALARA concept at all levels of RAM and jRPD use

i

The-Radiation Safety Officer, through the JtSOF staff , will bconduct radiation level and contamination : surveys and safety jaudits-of each laboratory or . area authorized for use of '

licensed material on a semi-annual schedule. These ' surveysare conducted- to confirm authorized user'(AU)-surveys. The

-

audit portion of the RSOF inspection is intended to verify:a Authorized user inventories i

<

a RAM use, storage and disposal

a Radiation worker training i

a Protection and monitoring7

a that records maintained in each use area aresufficient to meet all requirements of licensesand permits.

1

CONTROL No. 8 9 417.a

--. . .

*.,.

^1,. .-3-..

d

i 2. Assistant Radiation Safety Officer (ARSO)

L The ARSO reports.directly to the RSO and, in general,provides the day-to-day direct supervision. of the Radia-

- tion Safety Office staff. The ARSO's basic functions and *

responsibilities are as follows:

- to supervise the daily technical operations of theRadiation Safety' Office (RSOF)

- to provide orientation, training and reviews of theRSOF staff +

- to supervise the receipt, surveying and distributionof radioactive material packages and the associated

,

'

recordkeeping

- to prepare and inspect radioactive wastes.and out-p going radioactive material packages and complete allE related documentation of these transfers

- to supervise the maintenance of inventories of radio-active material

.

- to supervise the calibration and maintenance ofportable radiation detection instruments

- i

! - to supervise the performance of in-house personnel| dosimetry services 1

- to supervise the performance of audits of all usesand use : areas of radioactive material and radiation- ,

producing: devicesI

- to supervise the monitoring of therapy patient rooms- to supervise Univerisity Radiation Safety Training

programs for authorized users, radiation workers,ancillary personnel and patient care personnel 1

| - to supervise decontamination projects- to supervise. the radioactive material purchase

approval program

The ARSO, as a minimum, will possess a Bachelor of Sciencedegree in the physical or biological sciences (with emphasison health physics) or nuclear engineering. In addition, theARSO must have'3 to 5 years of health physics-related exper-ience. The ARSO assumes full authority and the responsibili-ties of the RSO in the RSO's absence. This includes theauthority to suspend activities when warranted.

- . .- _ _ _ __ __ _ _. . . ___ _ _

=y5,_. ...

>| A. r . .. ..y

,

,,/ Victoric R. M2rris, '

931 Cat Hill-RoadSellersv111e..PA 18960

Home phone: (215) 723 7333Work phone: (215) 661 7926

EDUCATION

[ M.S Health Sciences, Purdue University,. West Lafayette, IN 47907Area of Study: Health Physics / Industrial HygieneThesis: Initial Test Results of the Purdue University Radioactive.Waste Incinerator|.

B.S. Environmental Health, Purdue University, West Lafayette, IN 47907.

CERTIFICATION

Certified Health Physicist - American Board of Health PhysicsEMPLOYMENT' HISTORY

Staff Health Physicist - Radiation Safety Officer, Merck Sharp and DohmeResearch Laboratory ( May 87 - Present)*

Management of the operational radiation safety program for the West PointL site. (Includes supervision of 2 staff members and dealing with

supervisors of union personnel who har.dle radioactive material)* Conduct radiation ' safety training courses.Oversee on site radioactive vaste processing (incineration and decay). I*

Review applic.ations to use radioactive material, document any additional ';*

information or requirements, then present application with to the !

Radiation Safety Committee or appropriate subcommittee. !4*

Assist in preparing re application and amendments of NRC licenses.*Oversee bioassay and dosimetry program. Includes contacting andcounseling users about uptakes or exposures.

. Write departmental and user standard operating procedures.*

*: Oversee off-site shipments of radioactive material...

|'

Assistant Radiological Control Officer, Purdue University West Lafayette,I

Indiana (1984-1987)p *

Management of University Radiological Services Office (includes direct or'

indirect supervision of 5 staff members and 6 - 18 students) j

*Review and prepare applications to use radiocctive materint for approvalby the University Radiological Control Committee.

* Assist in preparing re-application and amendments of NRC licenses,* Conduct user training sessions.i'

. Write departmental standard operating procedures,*

i - Health Physicist, Purdue University West Lafayette, Indiana (1979-1984)b Review with users of radioactive material methods to minimize risk.

*

|, *. Management of University radioactive waste operations.

* Training of student interns and radiation safety technicians.Calibration of radiological control instruments.*

* Conduct radiation safety surveys of laboratories and x ray units.

Laboratory Technician. Lafayette Wastewater Treatment Plant lafayette,j1

. Indiana (1978-1979)

Vastewater Laboratory Technician Anheuser Busch Laf ayette. Indiana (1977)I''

CONTROL NO. 8 9 417l

_

[' . . . , ,. . , _ . ...y.... . ,j .q., , . .,-,

[ 'g y ,. * * . .

1..

. -; ..

'' society MEMaEREWIPS1'

Health Physics! Society

Delaware Valley: Society for Radiation Safety -

Hoosier Health Physics Society (Councilmen 1981-82)

ACADEMIC AND PROFESSIONAL HONORS

Sigma Xi=s

Eta Sigma Cammae9

i

PUBLICATIONS

* Evaluation of a Small Inexpensive-Incinerator for InstitutionalRadioactive Waste", Health Physics, Vol. 44-6, June 1983,pp. 671-675.,

REFERENCES UPON REQUEST

.

|

io

'Ii

o e. .

i

4

$

j.-

:

ii

' , .I;

!

i

.

.

. , . .. . . . . |

y . .

CD1UUCULUMVITAE * *

JAMESI. IPRRAMD, Ph.D., ..

. ..,

PERSONAL'

Born: March 9 1943, Eau Claire, Wiscons inMarriod: 1965;2 childrun 21 and 23

,

Offtee Bome.

Address: Division of Basic Science Research 8875 Blackwood CourtChildren's Hospital Research Foundation Cincinnati,OH 45236Elland and Betaesda AvenuesCincinnati,OH 45229

Phone: (513)55S4549 (518)793-0573.

' EDUCATION

1985 B.S. Chunistry, Marquette University, Milwaukee, Wisconsin.'

, 15r10 Ph.D. Bianbe=4try, Marquette University and Marquette School of Medicine,Inc. (now Medical College of Wisconsin) Milwaukee, Wisconsin.

t

PROFESSIONALAFFRJATIONS

American Chemical SocietyAmerican AWation ihr the Advancement of ScienceSigmaX1Sigma' Gamma Chi (Marquette University Chamistry Honor Society)New Yock Aandemy of Science

. American Society ofCell Biology,

HONORS, AWARDS,REVIEWCOMMITIEES

Academic Scholarship, Marquetta University ,1961-1965Public Health Service Pr=1Macal Fellowship Grant ,19681969PMMmalFellowship, Roche Institute of Malan=lar Biology,1969-1971

-

'

Pharmaceutical Manufado-. Association Foundation'

Pharmacology-Morphology Fellowship,1972-1974 ,

Ad Hoc Reviewer for Molan nler Cytology Study 86ction,1984Member, American Heart Amaaeintion (Ohio AMiliate)

Sdentific Review Committee,1988-1990Ad Hoc Reviewer, Journal of Biological Chemistry, Journal of Cell Biology,'

Developmental Biology'i

PROFESSIONALEXPERIENCE

1965-1909 Prad~4aral Research Assistant, Blochamlatry Departmant, MarquetteMedical School, Inc. Graduato advisor, Dr. F. Taketa.

-

19841971 Postdoctoral Fellow of the Roche Institute of Molecular Biology Nutley, .New Jersey. Sponsor, Dr. Sidney Pestka.,

.

1,

CONTRO!.No 8 9 417.

... . . . - . - . . . . _ . . . . . . . . . . . .

. . _ . . . . . . . . -. .. .

E, CliRRICULUMVITAE JAMES L.LESSARD,Ph.D,~

. ~*

.

4

,,.i ' 15r11-1972

Research Scholar, Children's Ifospital Research Foundation,l

Cincinnati, Ohio. Supervisor, Dr. Ernest F. Zimmerman.

197E1974 Fellow in Pharmacology-Morphology, Pharmaceutical ManufacturersAr.sociation Foundation. Sponsor, Dr. Ernest F. 7dmmerman.,

Ifr/MS79 Assistant Pmfassor of Rar.earch Pediatrics, University of CincinnatiMedical School.

1972-Present Staff Scientist, Institute for Developmental Research, Children'sHospital Research Foundation, Cincinnati, Ohio,

1972-PresentFaculty of the Developmental Biology Graduate Program, University of,, Cincinnati.

1978.PresentMember of the University wide Graduate Faculty, University ofCincinnati

1974-Pseaant*

Assistant Professor of Biological Chemistry, University of Cindanatii

1979 Present Jamadate Professar of Pediatrics.

1981-Present Director, Core Man ~1on=1 Antibody Facility

1986-1989Director af Graduate Studies, Developmental Biology Graduate"reirma, University of nin-mn.ti.

1989 PrenantDireciar, Developmental Biology Greduste Program, University ofCincinnati.

!

'COMMITmen *i

CidMrenhHospitalMadiaalCenter

1981-Phaeant Chairman Hazardona Materials Committee1987 Present Mamhar, Biohamed Safety Om=mittaa

',

1987-Premant Member, CHMC Bafety Ca==Jtteel

UnivetuityofCincinnail I.

11r151980 Member, Medical Sciences Committee!'

'19841987Member, Reappointments, Promotion and Tenure Committee,Department of Pediatrics, University of Cincinnati

i

19d8Chairman, Reappointments, Promotion and Tenure Committaa,Department of Pediatrics, University of Cindnnati,

"

1989-Present Member, Radiation Safety Committee, University of Oindnnati1989 Present Mambor, Radiation Safety Task Force, University of Cincinnati

.

2',

- e

. . - - - . ..... .. . . . . .. .

.

-... -

. l. .

CURRICULUM VITAE JAMES L.LESSARD,1%D,,

- - *, .

-. .

\'

PUBLICATIONS|1i

1. Taketa F., Smit.s, M.R., Dibona, F.J. and Lessard, J.L St.udies on cat homoglobinand hybrids with human hamoglobin A. Blochomistry 6:3809-3819 (1967).

.

, 2. Taketa, F., Smits, M.R. and Imssard, J.L Hemoglobin heterogeneity in the cat. Ii

Biochem. Biophys. Ros. Commun. 30:219 226 (1DG8). I

' 3. Lossard, J.L. and Takata, T. Multiple homoglobins in fetal, newborn and adult mis. l!

Diochem. Biophys. Acta.175:441-444 (1909). ;

\-

4. Hishizawa,' F., Lossard, J.L and Pestka, S. Studies on the formation of transfer |ribonucleic acid ribosome complexes. XIL Phenylalanyloligonucleotide binding to ;,

R. coli ribosomes: necessity for a fmo umino group. Proc. Natl. Acad. Sci. USA]66:523430(1970).'

|;5. Pestka, S., Hishizawa, T. and lessard, J.L. Studies on the format. ion of transfer !

ribonucleic acid-ribosomes complexes. XUL Aminoacyloligonucleotide hinding toribosomes: chameteristics and mquirements. J. Biol. Chem. 245:6208-6219 (1970). -

,

6. Taketa, F., Mank, A.G. and 14ssard, J.L. Chain amino terminal of the cathemoglobins and the naponse to 2,3-diphosphoglycerate and adenosine |

-

tripha=nh=te. J. Biol. Chem. 248:4471-4476 (1970). 1

!

7.' 14ssard, J.L. and Postka, S. Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIL Binding aminoacyloligonucleotides to ribosomes. J.

,

Biol. Chem. 247:69316908 (1972)..

8. 'Lessard, J.L. and Pestka S Studies on the formation of transfer rihaandeic add-, . -

ribosome complexes. XXIIL Chloroamphenicol, aminoacyloligonucleotides, andKachaichia ooit dbosomes. J. Biol Chem. 247:6909 6912 (1972).

9.~ Lessard, J.L., Wee, E. and Zimmannan. E.P. Presence of contractile proteins in'

mouse fatal palates prior to shalf elevation. Teratology 9:118-126 (1974)..

10. Whitsett, J. and Iassard, J.L. Characteristics of the microvillus brush border ofi

human placenta: insulin receptor localization in brush border membrane.{

,

Endocrinology 108:14581468 (1978). .

|,

' 11. Iassard, J.L., Rain, D., Carlton, D. and Akeson, R.A. A solid. phase assay forantiachin antibody and actin using Protein-A. Anal. Biochem. 94:140-149 (1979).

,

12. Williams, D.A., Iessard, J.L., Boxer, L.A., Rain, D. and Baehner, R.L. Metabolicand structural mquirements for concanavalin A capping in phagocytic cells. Proc.Soc. ExptL Med. BioL162:34-37 (1979).

|

13. Rain, D.C., Gruenstein, E. and Lessard, J.L. Synthesis of myosin and actin ini diferentiatingneuroblastoma cells. J.Neurochem. 34:1459-1469 (1980).

14. Kuhn, M., Babiazz, B.S., lessard, J.L. and Zimmerman, E.F. Palate morphogenesis:', L Immunological and ultrastructural analyses of mouse palate. Teratology 21:209-223(1980). 1

,

. ,

.

I

a|

.

.. . . conmot No. 8 9 4 3 g i-

|. . . . . . . . . . . .. . . . . . , . . = _ . _ . . . . ... ....... ,. = .= ..

.. - . . .__ _ -- . - - . _ . - .

( , .CUlkRICdLUMVITAE___ - - . - -

y__

JAMES L LERRARD,Ph.D, '- '

; '15.I4asard, J.L IIybridoma antibodies: new tools for old[A Peace Marcell Dek$'erblems. In: Nonisoto cAlte atives to Radioimmunoassay. Ed., L. Kaplan an

,

. , ,, ,

!! 16. Sadlar, T.D., Greenberg, D., Coughlin, P., and lessard, J.L Actin distribution; patterns in the mouse neural tube during naurulation. Science 21E172174 (1982).I

17. Ball, W.J., Schwartz, A. and Lessard, J.L. Isolation and characterir.ation of|

i

monebal antibodies to Na K+-ATPar.o. Biochem. Biophys. Acta 719: 418 423 (1982).4| i

|| 18. Whilaett, J.A., Hull, W., Dion, C., and 14ssard, J. c AMP dependant actin ;; phosphorylation in deve?oping rat Inng and type II epithelial cells. Exptl. Lung Res.,); 9:191-201(1985).j'

the gamma,' isoform of non muscle actin in cultured cells. J. Cell Biol.1022726-87Otey, C.A. Kalnoski, M.H., Isssard, J.L., and Balinski, J.C. Immunolocohation ofl

19. I

1'; G986).| |

, 20. Moyer, S.A., Baker, S.C. and Lessard, J.L Tubulln: A factor n-ary for the!

''

. synthesis of both Sendai virus and Vesicular stomatitus virus RNAs. Pme. Natl.Acad. Sci., USA 83:5405-6409 G986).

George, J.F., Karp, R.D. Rallahan, B.L. and Lessard, J.L Alteration of the protein2Lcomposition in the hamn, lymph of American cockroaches immunised with solubleproteins. J ImmunoL 62:505-509 (1987).,,

'122. Gomes, R.A., Sturgill, B., Chevalier, RL., Lessard, J.L, Owens, GX and Peach,

M.J. Expression of muscle s '

!=a=Ws in multiple organs of the fetal rat. CellandTissus Res. 250:7-12(1i

.

23.~

H. The EfEurential e5 mets of TPA on myo,sbrils and stress fibers in myotubes andIin, ZX Eshleman, J., Forry.Schaudies S., Duran, S., Lesnard, J.L. and Holtser,*.

replicating mononaciented calls. J. Cell Biol.106:18851876 (1987). |'

'

,

44 Lassard, J.L Two man ~bal antibodies to actin. one generally rosative and onemuscle adective. CeR Motility and the CyWi=laton.10:849-862 0988).

4

25, Wang S., Greaser M.L. Schulz, E., Balineid. J.C., Iin, JJ.C., and Lassard, J.Studies on cardiac myaAh iNg=nesis with antibodies to titin, actin, tropomyosin

- -

'and myosin. J. Cell Biol.107:1075-1083 G 988).

26. McHugh, R.M. and IAaaard, J.L. Nucleotida sequence of a rat vascular alpha-actinCDNA. Nucleic Acids Res.16:4167 G 988).

27. Sawtell, N.M., Hartman, A.L. Saucsik, J.C., and Lossard, J.L. Imme-h=micalstudies of h=41n expression in intestinal epithallai and cultured muscle cells. |i

UCLA Symposium on the Molecular and Cellular Aspects of Muscle Development. |;

L Kades and F. Swwalg eds., Alan R Iiss, New York (1988) pp 147156.|

' 28. McHugh, K.M., Bmwn, C.W. and Lessard, J.L A molecular analysis of the rati

lstomach alpha-vascular, gam, ma-enteric, and gemma. cytoplasmic isoactins.'

UCIA Symposium on the Molecular and Cellular Aspects of Muscle Development. |-

|

iL. Kedes and F. Stockdale, eds., Alan R Liss, New York (1988) pp 157-168. '

..

1

4

.

.-, -

.

C4)NTROL NO. 8 9 4"l' 7~~

. . . . . - _ - _ . . . . . . . - . .

|'

- - _ . ---_ .. . - . . . - . -:: PT - - - - - - -

7p~,.. .. .

CURRICULUMVITAE JAMES L. LESSARD, Ph.D. '--,

,

, .,

29. Greaser, ML, Handel, S.E., Wang, W M., Schulz, E., Bulinski, J.C., Iin, J. J-C.,and Lessard, JL Assembly of titin, myosin, actin, and tropomposin into myofibensin cultured chick oartliomyocytes, UCIA Symposium on Molecular and Cellular

'

Biology, Vol. 93, L. Kedes and F. Stockdale, eds., Alan R. Lias, New York. (19S8) pp2472rA

ad. McHugh, K.M. and lassard, JA The developmental arpression of the rat alpha- '

vascular and gnmma-enteric smooth muscle isoactins: Isolation andcharacterization of a rat gamma-enteric actin CDNA. Mol. Cell. Biol.12:5224-52310 988).

,

.

81. Sawtell, N'.M., Hartman, A.L., and Insard, J.L. Expression of unique actinisoforms in rat intestinal epithelial cells. Cell Motility and the Cytoskel.11:318 325(1988).

,. ,

32. Handel, S.E., Greasar, ML, Wang, S.M., Schultz, E., Bulinski, J.C., Lin, J. J-C.,and Im-rd, JL Myo5brillogenesis in chick cardiac myocytes as studied withantaW against muscle and nonmuscle proteins. Dev. Biol.132:35 44 (1989).

,

33. Hartman, A.L., Sawtell, N.M., and Lessard, J.L. Differential expression of actin,

isoforms in developing rat intestinal epithelial cells. J. Histochem. CyMam- i

87:t225-1288(1989).,

80 IWazi, Y.M., I.essard, J.L., and Weiss, M.A. Use of anti actin and 8100 proteinantibodies in differentiating benign and malignant schlorosing breast lesions. J.Surg. Path 2:125-135(1989). ;

4

35. Sawtell, N.M., Hartman, A.L. and Lessard, J.L. Cellular distribution of smoothmuscle actins during mammahan embr7ogenesis: Expression of the a vascular

'

but not the v.entarie W~ m in differentasting striated myocytes. J. Cell Biol. <

109:202EN29870989). Ii4

86. Sawtell, N.M., Lassard, J.L., and Hartman, A1. The epitope pattem of two unique -

actins in intestinal epithalial cells is highly conserved. J. Erpt1 Zool., in press

' 37, i Rudnicki, M.A., Sawtell, N.M., Rauhl, K.R., Berg, R., Craig, J.C., Jardine, K.,'

I.easard, J.L. and McBurney, M.W. Smooth muscle actin arpression duringambryonal carcinoma differentiation in cell culture, J. Cellular Physiol.142:89-98 i

.

(1990). ''

,

88.

|Brown, C.W., McHugh, K.M., and Lessartl, J.L. Nudeotide sequence of a ratcyotplasmic gamma-actin CDNA. (in preparation).

1

'

,a

'I i

|

t4'

| |

, ..

{

l

'

5

.*4 . .

De eu. g84._ _ _ SW.G.h WD..E.pt. 8 WD*

4

-+ - -- - - ,~- - - - - - - - , ,--- - , , , - - , ,~. ---r---- m -

._ _ _ _ . . _ y

CdlAUCULUMVITAE JAMES L LESSARD,Ph.D. ', . .

,

|.- *

m t

!1. | Lessard, J.L. Smits, M.R., Dibona, F.J. and Taketa. F. St.ructurnt and functional!

'

properties ofcat hemoclobin. Fod. Proc. 20:829 (1967). Jj

2. Iassard, J.L, Hishizawa, T. and Pastkn, S. Aminoacyloligonucleotido binding to !ribosomes and the effects of antibiotics. Fed. Proc. 20:899 (1969)..

,

3. Pestka, S., Hishizawa, T. and Lessard, J.h Binding of the aminotarminus nf' aminoacyl-TRNA to ribosomes. 8th Intern. Cong. Biochem. Abst. 207: (1970).

A Lessard,<J.L and Zimmerman, E.F. Actin in mouse fetal palates. Teratology |.

5: Abs.2160 (1972).'

5. Rein, D.,'Carlton, D. and lassard, J.L. Actin and myosin in marineneumblastoma cells. J. Cell Biol. 67:350a (1975).e

6.' Lessard, J.L., Goldblatt, D., Rain, D. and Carlton, D. T-li== tion of actin in i

nemobhtoms wIl. by immunofluorusomace. lat Intern. Cong. Cell Blot J. CellBiot 70:150a(1976). >,

7. Kuhn, E.M., Bablarz, B.S.,14ssard, J.L. and Zimmerman, E.F. PalateMorybogenesis: L r~mli =_ tion of non-muscle contractile systems by-

;

immunofluoresonnee and electron microscopy. Presented at the 5th Intern. Conf.Birth Defecta1977. |

8. Lessard, J.L. Rain, D.C., Carltan, D. and Akasan, RA. A solid-phaseradioimmunoassay for actin and antiactin igg. J. Cell Biot 75:276a (1977)..

9.'

Willl===, D., Boxer, L., Lassard, J.L, Rein, D. and B -h==e, R Diffemnoes in *

cy+aa-1==in B and metabolic requirements for maa=n=valin A capping in ruineapig polymarah==d--- leukocytes, alveolar macrophages, and peritonaal - .,

-

macrophases. J. Cell Biol 79:261a (1978).'

10. I4ssard, J.L., Edelbrock, C., Milostan, F. and Carlton, D. Hybridoma and rabbitantibodies against chicken muscle actins: Specificity and cross-reactivity. Europ. J.Cell Biol.22:817aG980).

'

11 h m A. and Lassard, J.L. Actin associated proteins in P3X88 Ag8 myeloma *

'

calls during immunoglobulin patching. J. Cell Biol. 87:100a (1980).,

12. Lassard, J.L. Antibody specificity toward muscle actins correlates with amino acid )evaanaos near the amino terminal. Fed. Pme. 41:1416a (1982).

'

>

18. Wilit==, D.A., Rothbaum, RJ., Daugherty, C.C., Lessard, J.L. and Harris, RE. |Polymorphonuclear leukocyta (PMN) cyfnahalatal abnormalities in Schwachmansyndromes (SS). Pediatr. Res. 16:218a (1982).

It i Dingle, S., Akoson, R and I4ssard, J.L. Diffemntial antibody response amongmouse strains to guinea pig leukemia cell surface antigens and actin. Hybridoma1:197(1982). 1

i

' 15. 14ssard, J.L. and Edelbrock, C. Hybridoma antibodies to gizzard actin binding to.cpitopes that are eithar unique or shared with other actins. Hybridoma l:198(1982).

'G

M UROL}O. b y 41 [-

'. .. . ..

,

. . . . .. . . . . _., . . ~ . . . . . .

-) -

.-_ -__ :. a_ : - _. _. .- _ ._ .. . . - .

-.

CURRICUI. AIM VITAE JAMES L LESSARD,Ph.D.. . '

. . .'

16. Stein, E.A., McNearey, C., I4ssard, J., Kashyup, M.L. Smith, R.D., and Pesoe, A.*

Prwiuction and evaluation of monoclonal antibodies to human apolipoprotein AILPresented at the 55th Scientific Session of the American IIoart Association.,

17. 14ssani, J.L, Carlton, D., and Edelbrock, C. Characterization of monoclonalantilxxlios to muscle actins. Fed. Pme. 42:2218 (1983).

18. Lessard, J.h, SchefIler, S., Engel, L., and Tepperman, K. Imruunofluorescentlocalization of actins in diffenntiating chick myoblasts. J. Cell Biol. 74:285 (1983).,

19. 14ssard, J.L. Characterization of monoclonal antibmlies to muscle actina. Fed.Prte. 42:2218(1983),

t

20. Lassard, J1 Monoclonal antibodies to artarial actins. International Cell Biology,Seno, S. and Okada, Y., eds. Academic Press, Tokyo, Japan, p. 515 (1984).

2L i Lessard, J.L. and Sadler, T.D. Developmental expression of three actin epitopes inthe rat. J. Ceu Biochem. Suppl. 9B,40. (1985).

22L Greasar, M., Bulinski, J., Lin, J. and I4ssard, J.L. Immunolocalization of titin-

and actin in cultured rat cardiac myocytes. J. Call Biot 103:125a (1980).-

23.| Hartman, A1, Sawtall, N.M. and lessard, J.L. Differential erpression of actinisoforma in developing rat intestinal epithelial ceHs. J. Histochem. Cytochem.89:1019(1987).

.

i 24. Sawtall, N.M., Hartman,A.L. and Lessard, J.L. Suboellular distribution of,

" muscle" actin in adult rat intestinal epitheHal cells, J. Histochem. Cytochem.89:1018(1987).i

25. Smoosik J.C. and Lessard, J.L. Developmental Expression of the muscle isoformsof creati,ne kinase and actin in cultured chicken myoblasta. Fed. Proc. 46:2129 'v,

(1987).'

.

26. Sawtmllisoforma, N.M., Hartman, A.L. and lessard, J.L. Expression of unique actin

in rat intestinal epithelial calls. Fed. Proc. 46:2015 (1987).i

57. Sawlall, N.M., imamard, J.L. and Hartman, A.L. An immunologically definedmarker of actin isoform divargence. J. Cell Biot 105:26a (1987),, ,

28. McHugh, E.M. and Lessani, J.L. Isolation of a unique actin specific CDNA clonefmm rat st==h. J. Cell Biot 105:26a (1987)..

29. HUazi, Y.M., lassard, J.L., and Weiss, M.A. Use of anti actin and 8100 protainantibodies in differentiating benign and malignant aclerosing breast lesions. U.S. :

and Canadian Academy of Pathology, March 1988.,

30. Sawteu, N.M., Lessard, J.L., Richardson, B, Dingle, S., and Hartman, A.L. Theepitow pattern of two unique actins in intestinal epithelial cells is highly conserved..

J. Ce"InlarBiochemistry Suppt 120,844a. (1988). .

j

..

81. Greaser, M.L., Handal, S.E., Wang, S-M., Schulz, E., Bulinski, J.C., Lin, J. J-C.,and lessard, J.L. Assembly of titin, myosin, actin, and tropomyosin into myofibrils t

in chick canilomyocytes. J. Cellular Biochem. Suppl.120 338a. (1988).,

!

7

.

. .

.,. = - . =. ..

I ~ '

. -. . .*

y ,;- comucumavrrAE JAMES L.LESSMtD, Ph.D.-

t

32. Srucsik, J.C., Ayra, G., and lessard, J.L. Differences in the developmental,

expression of the muscle isoforms of creatine kinase and actin in culturedmyoblasts. J. Collular Biochemistry Suppl.120,345a. 0988).

33. Brown, C.W., McHugh, K.M., and Imtsard, J.L. Alternative 3'-untranslatedregions in two rat cytoplasmic y actin CDNA clones. J. Cellular Biochemistry Suppl.,

120,348a. (1988).

t 34. McHugh, K.M., Crawford, K., and lossard, J.L. Isolation and characteriration of-

smooth muscle al(Suppt 120,373a)pha and gamma actin cDNAs in rat. J. Cellular Biochemistry(1988).

35. Brown, O W., McHugh, K.M., and l4ssard, J.L. Two mRNAs differing in their 3'-

untranslated regions encode cytoplasmic y-actin. J. Cell BioL107:252a (1988)..

30. Sawtell, N.M., Hartman, A.L. and Lessard, J.L. The smooth muscle a-actin istransiently expressed in rat developing embryonic striated muscle. J. CallBloL107:252a(1988).

4

37. I-rd, J.L., Crawford, K., Ayra, G., McHugh, K.M., and Richardson, B. The,

relationships amon the actin hinding sites for monoclonal antibodies, Dnase I andactin. J.CellBioL107:33a (1988).,

i .

,

|'

.

!

l

.

t I

.

| t'

to,

.;.

I i;

.

!

i t!,

/;.

.

,

',

'

8.

'

GQNTROLNo. S 9 417i

- -- - -

,.-,