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UNMODERATED POSTER SESSIONS

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P-3.042linical Observation of Prophylactic

ntravesical Instillation of PirurubicinTHP) in the Effects of Postoperativeuality of Life in Patients withuperficial Bladder Cancerheng Z, Li M, Chen X, Yan M, Yang Xeijing Cancer Hospital, Beijing, China

ntroduction and Objective: To evaluatehe effects and safety of prophylactic in-ravesical instillation of pirurubicin (THP)o superficial bladder cancer after TUR-Btr bladder partial resection.aterials and Methods: Eighty-seven

atients were selected, and all these pa-ients had received TUR-Bt or bladder par-ial resection because of superficial blad-er tumor, and they were followed up for-60 months. After the operation, THP30mg/30ml) was instilled into bladder,hen stayed for 30-45 min. In the follwingight weeks, intravescial instllation ofiruruvicin was administered once aeek. After the instllation for eighteeks, it was done once a month for

ight months. And these patients ac-epted cystoscopy per three months, ifound recurrence, they should have an-ther operation.esults: In our observation of 87 pa-

ients, we found 19 patients had urinaryrritation symptoms, 14 felt bladder un-

ell, 5 had dysuria, 4 was sleepless andemarkably 17 had psychological alter-tion for warrying about bladder instilla-ion, cystoscopy, recurrence and metasta-is.onclusion: Intravesical instillation ofirurubicin is an effective method to pre-ent recurrence, but the side effect wasainly local reaction, and will obviously

elieve in short time after therapy. How-ver, we should pay attention to someatients with psychological alteration, wehould take suitable method to instill piru-ubicin and have correct instruction toliminate their worries.

P-3.043igh Glucose Concentration Increaseuman Prostatic Stromal Cells (HPSC)ontraction Mediated by Proteininase C-Alpha (PKC-Alpha) Isozymectivityurnomo B, Wayan Widharse, Aulani’anepartment of Urology, Medical Schoolrawijaya University, Saiful Anwar Hos-ital, Malang, Indonesia

ntroduction and Objective: Severaltudies have suggested a specific associa-ion between severity symptom of benignrostatic hyperplasia (BPH) and diabetes

ellitus. Some studies have also proved p

ROLOGY 74 (Supplment 4A), October 2009

hat increasing protein kinase C (PKC)lpha isozyme activity after glucose expo-ure is a factor that increasing contractionn vascular smooth muscle cell culture.ncreasing contraction of prostatic stromalells was known as dynamic componentf prostatic obstruction. This in vitro ex-eriment proved that glucose treatment inuman prostatic stromal cells (HPSC) cul-ure would increase PKC alpha isozymectivity.aterials and Methods: Primary cultures

f HPSC were obtained from patients withladder outlet obstruction caused by be-ign prostatic enlargement. The cellsere incubated in various concentrationsf glucose (5, 10, 15, 20, 25, 30, 35 mi-ro-Mol) for 48 hours. The activity ofKC-alpha isozyme from various glucoseoncentrations were examined quantita-ively by spectrophotometry methods. Theffects of glucose on cells contractilityere examined by counting the percent-

ge of cells that contract after administra-ion of 1, 10, and 100 micro-Mol of phen-lephrine.esults: Exposures to various doses oflucose resulted in increased isozyme ac-ivity. The increment of activity is linearith the increment of glucose dose.here is a strong correlation between glu-ose doses and PKCalpha activity, with2�0.91. The result showed that adminis-

ration of 10 micro-M ol phenylephrineive the strongest contraction effect (54.37%) compared to 1 micro-Mol (34 �.37%) and 100 micro-M ol (25.75 �.22%). The percentage of contraction

nduced by various dose of phenylephrineould be increased, if the cells incubated

n 20 micro-Mol glucose for 48 hours be-ore phenylephrine administration. Theercentage of contraction would in-reased to 14.25 � 3.03% after exposureo 1 micro-Mol phenypephrine (p�0,002);0 � 3.03% after 10 micro-Mol pheyleph-ine (p�0,000), and 7.5 � 3.03% after00 micro-M ol phenylephrine (p�0,183).onclusions: At different concentrations,lucose increases HPSC contraction, medi-ted by PKCalpha isozyme.

P-3.044enign Prostatic Hyperplasia:istologic Confirmation of an Ever-rogressing Diseaseela-Navarrete R1, Manzarbeitia F2,arasa J2, Fernadez Acenero M2, Gonzaleznguita C1

Urology, Fundacion Jimenez Diaz Uni-ersity Hospital, Madrid; 2Pathology,undacion Jimenez Diaz University Hos-

ital, Madrid, Spain O

ntroduction and Objective: BPH mor-hogenesis starts from initial mesenquimalodules and myxoid primitive structuressually localized in the periurethral zonetransition zone). It has been supposedhat the younger is an individual the moref these structures would be identified inhe samples coming from TURP or opendenomectomy. We challenged this hy-othesis when looking for the role ofononuclear cells infiltration on theserimitive structures.aterials and Methods: Tissue samples

emoved from 70 patients who underwentURP were studied. The average age of

his population group was 70.8 � (57-97),ith an average tissue weight of 24.0 g

2-200 g). Areas of myxoid nodules oryxoid hyperplasia were identified during

outine evaluation and selected for specialtaining (Vimentin, smActin and Desmin)r processed conventionally using immu-ohistochemistry CD3, CD20, CD68, CD34.he number of myxoid nodules or myx-id hyperplasia was counted in each sam-le.esults: Myxoid and fibromyxoid nodulesere identified in variable proportion.any of them were localized in the subu-

otelial corion or interlaced with themooth muscle of the sphincter. The mostommon size was 0.2-5.0 mm. Next tohem were fibromuscular nodules of big-er size (�5mm), positive for Vimentin,educed expression of smActin and nega-ive expression of Desmin. A constant andniform infiltration of B and T lympho-ytes was present with occasional histio-ytes and mast cells. Glandular perme-tion at this stage was uncommon.urprisingly when compared the youngeropulation group (�57 years) with thelder (�80 years) it was discovered thathese initial mesenchyme structures wereore common in the older population

roup (0.33�0.7 vs. 2.00�2.7: p�0.02).onclusions: Progression of BPH in early

tages means a process of maturationrom myxoid and fibromyxoid nodules farefore glandular permeation is observed.ontrary to the expected decline of thisctivity with age, histology confirms thatPH is an ever-progressing disease.

P-3.045issue Effects of Greenlight HPS™ andvolve SLV™ Lasers on Caninerostates: An Acute In-Vivo Modelong C1, Spaliviero M1, Wolf R1, Kosanke

1, Chavez-Suarez M1, Broach F1, Araki M2

Department of Urology, University ofklahoma Health Sciences Center, Okla-oma City, USA; 2Okayama University,

kayama, Japan

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