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D Alert Created by# - the latest mycological papers 2015/08/24 Search date: from Atsushi Nakajima (@Ats_Nakajima) 20

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D AlertCreated by:Atsushi Nakajima (@Ats_Nakajima) CC BY 4.0# 20  - the latest mycological papers from Google Scholar

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20150824-1

Microchrysosphaera graminicola, an enigmatic new genus and species in theHypocreales from Panama

C Judith, AY Rossman, AH Kennedy, O Cáceres… - Mycological …, 2015 - Springer

イネ科/Poaceae, パナマ/Panama, 新種記載/new species, 新属提唱/new genus, 分子系統解析/molecular phylogenetic analysis

2015/08/24

An unusual fungus with a novel set of morphological, ecological, and DNA sequence characteristics was found to be causing necrosis ofdistal portions of living leaves and sterility of Parodiophyllochloa cordovensis (Poaceae) in Panama. The small, superficial, soft-texturedyellow perithecia develop directly on the surface of the necrotic plant tissue. The unitunicate asci are filled with hyaline, long-fusiform,typically 6–12-septate ascospores that disarticulate into secondary part-ascospores that later produce tertiary spores. This undescribedfungus could not be cultured, nor was an asexual state observed. Morphological characteristics are inconsistent with any existing genericconcept, but suggest a close phylogenetic relationship with species of Hypocreales. This was confirmed by molecular phylogenetic analysis ofnrLSU rDNA sequences. While host relationship and disarticulation of ascospores suggest an affinity between this fungus and species ofClavicipitaceae, its colonization of necrotic tissue of a living host, lack of stromata, non-thickened apices of the asci, and long-fusiform (notfiliform) ascospores suggest otherwise. Furthermore, this fungus differs morphologically from species in Bionectriaceae and Nectriaceae inits disarticulating long-fusiform ascospores, part-ascospores producing tertiary spores, and occurrence on living grasses. Our phylogeneticanalysis of combined ITS, nrLSU, and TEF1 sequences yielded evidence for placement of the new species within the Hypocreales, close toBionectriaceae and Nectriaceae. However, our analysis did not specifically clarify and support the species’ membership within either family.This fungus is described as Microchrysosphaera graminicola, a new genus and species belonging to the Hypocreales, incertae sedis.

(仮訳)パナマ産のボタンタケ目の謎の新属新種、Microchrysosphaera graminicola

http://link.springer.com/article/10.1007/s11557-015-1095-2URL

Hypocreales()、Clavicipitaceae()、Bionectriaceae()、Nectriaceae()

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20150824-3

Three genera in the Ceratocystidaceae are the respective symbionts of threeindependent lineages of ambrosia beetles with large, complex mycangia

CG Mayers, DL Mcnew, TC Harrington, RA Roeper… - Fungal Biology, 2015 - Elsevier

新種記載/new species, 養菌性キクイムシ/ambrosia beetle

2015/08/24

Abstract The genus Ambrosiella accommodates species of Ceratocystidaceae (Microascales) that are obligate, mutualistic symbionts ofambrosia beetles, but the genus appears to be polyphyletic and more diverse than previously recognized. In addition to A. xylebori, A.hartigii, A. beaveri, and A. roeperi, three new species of Ambrosiella are described from the ambrosia beetle tribe Xyleborini: A. nakashimaesp. nov. from Xylosandrus amputatus, A. batrae sp. nov. from Anisandrus sayi, and A. grosmanniae sp. nov. from X. germanus. The genusMeredithiella gen. nov. is created for symbionts of the tribe Corthylini, based on M. norrisii sp. nov. from Corthylus punctatissimus. Thegenus Phialophoropsis is resurrected to accommodate associates of the Xyloterini, including P. trypodendri from Trypodendron scabricollisand P. ferruginea comb. nov. from T. lineatum. Each of the 10 named species was distinguished by ITS rDNA barcoding and morphology,and the ITS rDNA sequences of four other putative species were obtained with Ceratocystis-specific primers and template DNA extractedfrom beetles or galleries. These results support the hypothesis that each ambrosia beetle species with large, complex mycangia carries itsown fungal symbiont. Conidiophore morphology and phylogenetic analyses using 18S (SSU) rDNA and TEF1α DNA sequences suggest thatthese three fungal genera within the Ceratocystidaceae independently adapted to symbiosis with the three respective beetle tribes. In turn,the beetle genera with large, complex mycangia appear to have evolved from other genera in their respective tribes that have smaller, lessselective mycangia and are associated with Raffaelea spp. (Ophiostomatales).

(仮訳)クワイカビ科の3属はそれぞれ、大型で複雑な菌嚢を有するアンブロシア・ビートルの独立3系統の共生菌である

http://www.sciencedirect.com/science/article/pii/S1878614615001397URL

Ceratocystidaceae()、Ambrosiella()、Microascales()、Phialophoropsis()、Raffaelea()、Ophiostomatales()

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20150824-4

Commercial Laurus nobilis L. and Syzygium aromaticum L. Merr. & Perry essential oilsagainst post-harvest phytopathogenic fungi on rice

MP Santamarina, J Roselló, S Giménez… - LWT-Food Science and …, 2015 - Elsevier

GC/MS, ゲッケイジュ属/Laurus, フトモモ属/Syzygium, 菌体の化学組成/chemicalcomposition, 抗真菌活性/antifungalactivity, 病原真菌/pathogenic fungi

2015/08/24

Rice is exposed in the field and in stored conditions to a great variety of fungi that can cause a lot of diseases with potential risk toconsumers. In the present study, the chemical composition of commercial Laurus nobilis L. and Syzygium aromaticum L. Merr. & Perryessential oils and antifungal activity against five pathogenic fungi isolated from Mediterranean rice grains has been investigated. Thirty-seven compounds accounting for more than 99.5% of the total essential oil were identified by GC and GC/MS. 1,8-Cineole (51.95%), α-terpinyl acetate (12.93%) and the monoterpene hydrocarbon sabinene (9.56%) were the main compounds in bay leaf essential oil, whilethe phenylpropanoid eugenol (88.58%), and the sesquiterpene hydrocarbons β-caryophyllene (8.13%) and α-humulene (2.35%) werefound in clove essential oil. Clove essential oils at 300 μg/mL showed more antifungal effect than bay leaf essential oil against all testedstrains. S. aromaticum essential oil showed the best antifungal activity towards Fusarium graminearum and similar antifungal activitycompared to pure eugenol against all tested phytopathogenic fungi. In inoculated rice grain, clove essential oil significantly reduced thefungal infection in food so S. aromaticum essential oil could be a good alternative as preservative in stored rice grain.

(仮訳)イネのポストハーベスト病原菌に対する商用ゲッケイジュおよびクローブ精油の効果

http://www.sciencedirect.com/science/article/pii/S0023643815301341URL

Fusarium()、Fusarium graminearum()

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20150824-5

First report of Neurospora on Corylus avellana in natural forest of Iran

K Rahnama, R Habibi - Journal of Yeast and Fungal …, 2015 - academicjournals.org

イラン/Iran, ハシバミ属/Corylus, 森林/forest

2015/08/24

Teleomorph stage of the fungus Neurospora udagawae was formed abundantly on the culture media of potato dextrose agar, potato carrotagar and malt extract agar. Perithecia were visible on the culture medium and plant tissues (on the bark of tree) of hazelnut tree (Corylusavellana L.) from specimens of the Northwest forest of Iran. The fungus was identified for the first time in this area. The isolate wasexamined for its macroscopic and microscopic features and identified as nonconidiating species of Neurospora. Growth rate of the fungushyphae was more than twice in 30 than 10 and 20°C. There was no growth at 5 and 40°C. This fungus is recorded here for the first timefrom hazelnut tree of Iran and so in the world.

(仮訳)イランの天然林のセイヨウハシバミに発生したアカパンカビ属菌の初報告

http://www.academicjournals.org/article/article1439642009_Rahnama and Habibie.pdfURL

Neurospora()、Neurospora udagawae()

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D AlertCreated by:Atsushi Nakajima (@Ats_Nakajima) CC BY 4.0# 20  - the latest mycological papers from Google Scholar

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20150824-6

Identification of commercial Ganoderma (Lingzhi) species by ITS2 sequences

B Liao, X Chen, J Han, Y Dan, L Wang, W Jiao… - Chinese …, 2015 - cmjournal.org

イラン/Iran, ハシバミ属/Corylus, マンネンタケ属/Ganoderma, 森林/forest

2015/08/24

Teleomorph stage of the fungus Neurospora udagawae was formed abundantly on the culture media of potato dextrose agar, potato carrotagar and malt extract agar. Perithecia were visible on the culture medium and plant tissues (on the bark of tree) of hazelnut tree (Corylusavellana L.) from specimens of the Northwest forest of Iran. The fungus was identified for the first time in this area. The isolate wasexamined for its macroscopic and microscopic features and identified as nonconidiating species of Neurospora. Growth rate of the fungushyphae was more than twice in 30 than 10 and 20°C. There was no growth at 5 and 40°C. This fungus is recorded here for the first timefrom hazelnut tree of Iran and so in the world.

(仮訳)ITS2配列による商用マンネンタケ属(霊芝)の種の同定

http://www.cmjournal.org/content/10/1/22/URL

Ganoderma()、Neurospora()、Neurospora udagawae()

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20150824-7

A method for obtaining RNA from Hemileia vastatrix appressoria produced in planta,suitable for transcriptomic analyses

A Loureiro, HG Azinheira, M do Céu Silva, P Talhinhas - Fungal Biology, 2015 - Elsevier

さび菌/rust fungi, 遺伝子発現/geneexpression, 定量PCR/quantitative PCR

2015/08/24

Abstract Appressoria are the first infection structures developed by rust fungi and require specific topographic signals from the host for theirdifferentiation. The ease in obtaining appressoria in vitro for these biotrophic fungi led to studies concerning gene expression and genediscovery at appressorial level, avoiding the need to distinguish plant and fungal transcripts. However, in some pathosystems, it wasobserved that gene expression in appressoria seems to be influenced by host-derived signals, suggesting that transcriptomic analysesperformed from in planta differentiated appressoria would be potentially more informative than those from in vitro differentiatedappressoria. Nevertheless analysing appressorial RNA obtained from in planta samples is often hampered by an excessive dilution of fungalRNA within plant RNA, besides uncertainty regarding the fungal or plant origin of RNA from highly conserved genes. To circumvent thesedifficulties, we have recovered Hemileia vastatrix appressoria from Arabica coffee leaf surface using a film of nitrocellulose dissolved in butyland ethyl acetates (nail polish), and extracted fungal RNA from the polish peel. RNA thus obtained is of good quality and usable for cDNAsynthesis and transcriptomic (quantitative PCR) studies. This method could provide the means to investigate specific host-inducedappressoria-related fungal pathogenicity factors.

(仮訳)植物体内部で形成されたHemileia vastatrix付着器からトランスクリプトーム解析に適したRNAを採取する手法

http://www.sciencedirect.com/science/article/pii/S1878614615001452URL

Hemileia()、Hemileia vastatrix()

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20150824-11

Why Nuclear Ribosomal Internal Transcribed Spacer (ITS) has been Selected as theDNA Barcode for Fungi

Z Mahmoud - Adv Genet Eng, 2015 - omicsgroup.org

DNAバーコーディング/DNA barcoding

2015/08/24

The application of DNA sequences of standardized genetic markersfor the identification of eukaryotic organisms is known as DNAbarcoding.Based on a recent study by a multinational andmultilaboratory fungal barcoding consortium, the nuclear ribosomalinternal transcribedspacer (ITS) has been selected as the DNAbarcode for fungi. DNA barcoding shows tremendous promise for theorganisms rapididentification at the species level. Recently, DNAbarcodes are used for identification of fungal species in marinesediments.

(仮訳)なぜ核リボソームITS領域が菌類のDNAバーコードに選定されたのか

http://www.omicsgroup.org/journals/why-nuclear-ribosomal-internal-transcribed-spacer-its-has-been-selected-asthe-dna-barcode-for-fungi-2169-0111-1000119.pdf

URL

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