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Unit 3
Cells and their Structure
The Cell Theory
• All living things are composed of cells
• Basic units of structure and function in living things
• Cells are produced from existing cells
Categories of CellsType 1 Prokaryotes
• Unicellular – one cell• No Nucleus – DNA
free floating• Example – Bacteria• Used in technique
called Recombinant DNA
Shapes of Prokaryotes• Cocci =
spherical (round)
• Bacillus = (rod shaped)
• Spirilla = helical (spiral)
What the heck is RecombinantDNA?
Recombinant DNA is what you get whenyou combine DNA from two different
sources.For example:
Mouse + Human DNAHuman + Bacterial DNAViral + Bacterial DNA
Human + (other) Human DNA
Why Make Recombinant DNA?Recombinant DNA Technology May
Allow Us To:• Cure or treat disease• Genetically modify our foods to increaseflavor, yield, nutritional value or shelf-life• Better understand human genetics• Clone cells or organs
Molecular Biology’s Best Friends: Bacteria
Why use bacteria?• They’re relatively simple organisms.• They reproduce very quickly and asexually (this means that the “daughter” cells will contain the exact same DNA as the “parent” cell).• It’s pretty easy to get DNA back into the bacteria after you’ve changed it.
Recombinant DNAThe foreign DNA is first joined to a small,
circular DNA molecule found in bacteria known as a plasmid.
Restriction enzymes
• A restriction enzyme (RE) is a specialized protein that cuts DNA in a very specific place.
• Molecular scissors
Steps to Recombinant DNAStep 1:
•Isolate (find) the human gene responsible for producing insulin and decide where you want to put it.•In this case, we decide to put our human DNA into the plasmid of E. coli, a very common bacterium.
Step 2:•Get the bacterial (plasmid) DNA out of the E. coli. We do this by basicallyexploding them.
Step 3:•Cut your human DNA and bacterial DNA with the same restriction enzyme
Step 4:•Mix the cut human DNA, which contains the insulin gene, with the cut bacterial DNA.•They’ll stick together because they were cut with the same restriction enzyme.
Step 5:Get your new recombinantplasmid back into the bacteria.This is easy because bacteria will take in DNA that’s floating around near them. We call this “transformation”.
Voila!!Now your E. coli will use its new DNAto make human insulin!Because they reproduce so quickly, you’ll soonhave thousands, millions, or billions of humaninsulin making machines.By filtering out the bacteria after they’ve madeinsulin, you’ve got clean human insulin that canbe packaged and given to diabetic patients.
A virus is an infectious agent made up of nucleic acid (DNA or RNA) wrapped in a
protein coat called a capsid.
Viruses have no nucleus, no organelles, no cytoplasm or cell membrane—Non-cellular
vs
This is why it does NOT belong to any kingdom.
Replication is how a virus spreads.A virus CANNOT reproduce by itself—it must invade a host cell and take over the cell activities, eventually causing destruction of the cell and killing it. (The virus enters a cell, makes copies of itself and causes the cell to burst releasing more viruses.) DNA/RNA is
copied.DNA/RNA injected
into cell.Virus attaches to cell.
Virus copies itself.
Cell bursts (lyses) and releases new viruses.
Step 1
Step 2 Step 3
Step 4
Step 5
Viruses have either DNA or RNA but NOT both.
Viruses with RNA that transcribe into DNA are called
retroviruses.
HIV Cell
A flea is a parasite to a dog and is harmful to the dog.
Viruses are parasites—an organism that depends entirely upon another living organism (a host) for its existence in such a
way that it harms that organism.
(This is the reason why HIV is so incurable.)
Categories of Cells Type 2 Eukaryotes
• Unicellular or Multicellular
• Has a Nucleus• Example – Plant,
Animal, fungi
How are Eukaryotes Different from Prokaryotes
Eukaryotes have a nucleus that contains DNA and Prokaryotes do not
Cell Structures in common
Cell Membrane Nucleus Ribosome
Endoplasmic
Reticulum
Vacuoles Mitochondria
Nucleus
• Controls most of cells processes
• Contains genetic information-DNA
• Chromosomes inside nucleus are the threadlike structures containing genetic information
Endoplasmic Reticulum
• Synthesizing, packaging and transporting of proteins
• Two types – rough ER and smooth ER
• Network of membranes
Golgi Apparatus
• Packaging system• Takes small molecules
and makes larger ones then stores them
• Proteins
Cell Membrane
• Regulates what enters and exits the cell
• Provides protection and support
• Semi-permeable – allows small substances through and keeps larger substances out
• Made of lipids and proteins