-
7/23/2019 uji bioassay & susceptibillity
1/20
5
BEB H
SMDLE@]K]ED
E$ Ke|er BmkeoednDmnere Hdljdmvhe imr}seoed veke` ve|} dmnere
bmromibedn xedn
imihkhoh ieveke` omvm`e|ed xedn ievh` f}o}s |hdnnh |mr}|eie
ieveke`
smdxeoh| imd}ker$ Omeleed |redvhvh Mshlmihjkjnh xedn lh|edleh
lmdned
vmieohd bmromibedndxe smdxeoh| lmnmdmre|hg led smdxeoh| imd}ker
xedn
bmk}i lese| lhe|evh vmsmd}`dxe imr}seoed vmbenhed |ed|edned
omvm`e|ed
lhieve lmsed$ Smdxeoh| imd}ker |mrvmb}| lhvmbeboed jkm` wmo|jr
smdxeoh|$
\ed|edned kehddxe xedn `er}v lh|ednn}kednh ed|ere kehd eleke`
imdhdnoe|dxe
ieveke` omvm`e|ed omrae+ omvm`e|ed khdno}dned+ led smr}be`ed
lekei bhledn
omsmdl}l}oed smdlhlhoed+ vjvhek b}lexe led leiseo nkjbekhvevh
xedn eoed
imibmrhoed smdner}` |mr`eles smromibedned omeleed omvm`e|ed
ievxereoe|$
\mroeh| lmdned ieveke` smdxeoh| imd}ker o`}v}vdxe xedn
lhvmbeboed
jkm` vmrednne+ ihvekdxe dxei}o+ smrk} eledxe |hdleoed smdedneded
xedn
|mse|$ \hdleoed }d|}o imi}|}v red|eh smd}kered smdxeoh| led
smibmred|eved
vmrednne vedne|ke` lhsmrk}oed$ \hdleoed smibmred|eved vmrednne
lese|
lhkeo}oed lmdned bmrbeneh fere vmsmr|h imdnn}deoed hdvmo|hvhle$
]d|}o
imdnm|e`}h omieis}ed hdvmo|hvhle lekei imib}d}` vmrednne
o`}v}vdxe
dxei}o lhkeo}oed smdn}ahed xedn lhvmb}| }ah bhjevvex$
Sjs}kevh v}e|} vmrednne xedn lhomdlekhoed+ sele }i}idxe
rmd|ed
|mr`eles hdvmo|hvhle xedn lhn}deoed }d|}o imibmred|evdxe$ Sele
bmbmrese
nmdmrevh+ omeis}`ed lerh hdvmo|hvhle h|} vmieohd imd}r}d vmbeb
vmrednne
vmieohd |jkmred |mr`eles hdvmo|hvhle led eo`hrdxe |hleo vmrednne
|mrvmb}|
imdaelh rmvhv|md |mr`eles hdvmo|hvhle xedn lhn}deoed (Brj~d led
Sek+ 58>5$
-
7/23/2019 uji bioassay & susceptibillity
2/20
2
\jkmredvh v}e|} vsmvhmv vmrednne |mr`eles hdvmo|hvhle vedne|
bmrenei+
lese| |mrb}o|h lmdned |mraelhdxe bmrbeneh srmvmd|evh omie|hed
bhke bmbmrese
omkjisjo vmrednne lerh vsmvhmv xedn veie+ lhseseroed lmdned
bmrbeneh ljvhv
e|e} ojdvmd|revh hdvmo|hvhle$ \jkmredvh h|} bmrohver ed|ere 3-
led 533- xedn
imr}seoed lhv|rhb}vh o}i}ke|hg djriek xedn lhvmb}| vmbered
|jkmredvh+
vmlednoed Iefdehr (5805 imdxmb}| lhggmrmd|hek
v}vfms|hbhkkh|x$
Eles}d omr}nhed smdnn}deed hdvmo|hvhle eleke` |hib}kdxe
nek}r)nek}r
vmrednne vevered xedn rmvhv|md |mr`eles hdvmo|hvhle$ Imoedhvim
rmvhv|mdvh
vmrednne |mr`eles hdvmo|hvhle lese| |mraelh imkek}h smr}be`ed
ghvhjkjnh led
smr}be`ed |hdnoe` keo} (JBrhmd+ 587>+ Brj~d led Sek+ 58>5$
Rmvhv|mdvh
ghvhjkjnh sele vmrednne imismrkh`e|oed omdxe|eed xedn kmbh`
smd|hdn$ @ek hdh
lhvmbeboed oermde geo|jr)geo|jr 5 lexe ebvjrbvh hdvmo|hvhle xedn
vedne|
keibe| vm`hdnne vmrednne |hleo ie|h? 2 lexe smdxhiseded
hdvmo|hvhle lekei
aerhdned xedn |hleo wh|ek+ vmsmr|h aerhdned kmieo vm`hdnne jrned
wh|ek |mr`hdler
led vmrednne |hleo ie|h? 6 lexe movormvh hdvmo|hvhle xedn fmse|
vm`hdnne
|hleo veiseh imib}d}` vmrednne? 7$
B$ R}i}ved Ieveke`5$ Eseoe` hdvmo|hvhle xedn lhn}deoed ievh`
mgmo|hg }d|}o imdnmdlekhoed
vmrednne
2$ Benehiedeoe` omrmd|eded vmrednne vevered |mr`eles bmbmrese
amdhvgjri}kevh hdvmo|hvhle
-
7/23/2019 uji bioassay & susceptibillity
3/20
6
F$ \}a}ed5$ Ie`evhv~e ieis} imkeo}oed }ah bhjevvex imlhe lmdned
bmder$2$ Ie`evhv~e ieis} imkeo}oed }ah bhjevvex lhdlhdn lmdned
bmder$6$ Ie`evhv~e ieis} imkeo}oed }ah v}vfms|hbhkkh|x lmdned
bmder$
-
7/23/2019 uji bioassay & susceptibillity
4/20
imi}|}v ie|e red|eh smd}kered LBL$ Gjnnhdn lhieov}loed vmbeneh
}sexe
imibevih dxei}o lm~eve (emlmv emnx|h$ Lh severed+ vee| hdh+
veke` ve|}
amdhv hdvmo|hvhle xedn lhn}deoed }d|}o imibmred|ev wmo|jr lmiei
bmrlere`
lmdn}m eleke` ieke|`hjd$
Ieke|`hjd imr}seoed hdvmo|hvhle njkjdned jrnedjgjvge|$ Fhrh
o`ev
lerh ieke|`hjd+ ed|ere kehd ieis} imk}is}`oed vmrednne lmdned
fmse|
lmdned imoedhvim imdxmredn vhv|mi vereg |mr}|eie sele vhdesvhv$
Fhrh
ieke|`hjd kehd+ imis}dxeh |jovhvh|ev rmke|hg rmdle` |mr`eles
ieiiekhe led
o}redn v|ebhk |mr`eles wmr|mbre|e$ Vmkehd h|} ieke|`hjd bmrvhge|
ojrjvhg|mr`eles kjnei+ bmrbe} o`ev+ vmr|e imis}dxeh red|eh oerbjd
xedn smdlmo$ Lh
severed bmd|}o ieke|`hjd eleke` fehr+ bheve lheskhoevhoed lekei
|`mriek
gjnnhdn$ Ijlm jg md|rx ieke|`hjd eleke` imkek}h o}kh|+
smrdegeved led
smdfmrdeed$
Dei}d smdnn}deed hdvmo|hvhle (}d|}o imibmred|evcd dxei}o
emlmv
emnxs|h xedn o}redn |mromdlekh eoed bmreohbe| |mraelhdxe
rmvhv|mdvh sele
dxei}o$ Vmfere srhdvhs imoedhvim rmvhv|mdvh hdh eoed imdfmne`
hdvmo|hvhle
bmrhoe|ed lmdned |h|ho |ernm|dxe e|e} |}b}` vmrednne imdaelh
ieis} }d|}o
imdn}reh be`ed eo|hg hdvmo|hvhle vmbmk}i veiseh sele |h|ho
vevered$
Vmlednoed amdhv e|e} |hdnoe|ed rmvhv|mdvh h|} vmdlhrh imkhs}|h
|e`es rmd|ed+
|jkmred ber} omi}lhed |e`es rmvhv|md$ Bmbmrese geo|jr xedn
imismdner}`h
imoedhvim rmvhv|mdvh hdvmo|hvhle sele emlmv emnxs|h hdh+ ed|ere
kehd 1
5$ Geo|jr nmdm|hf$ Geo|jr hdh |mrned|}dn sele ombmreleed nmd
rmvhv|mdxedn ieis} imdnojlm smibmd|}oed mdqhi |mr|md|} lekei
|}b}d
dxei}o$ Mdqhi hdh` eoed imdm|rekhvhr ombmreleed hdvmo|hvhle
(ihvekdxe mdqhi mv|mrevm$
-
7/23/2019 uji bioassay & susceptibillity
8/20
0
2$ Geo|jr bhjkjnhv xeh|} omfmse|ed rmnmdmrevh dxei}o emlmv
emnxs|h$Omieis}ed bmreles|evh |mr`eles |moeded ekei vmsmr|h
smibmrhed
hdvmo|hvhle led lhl}o}dn omfmse|ed rmnmdmrevh xedn |hdnnh
imdxmbeboed dxei}o fmse| imd}r}doed nmdmrevh xedn rmvhv|md$
6$ Geo|jr jsmrevhjdek imkhs}|h be`ed ohihe xedn lhn}deoed+
fereeskhoevh+ grmo}mdvh+ ljvhv led keie smieoehed$
Kea} smromibedned rmvhv|mdvh vedne| lhsmdner}`h jkm` |hdnoe|
|moeded
vmkmovh xedn lh|mrhie jkm` v}e|} sjs}kevh dxei}o Emlmv emnxs|h
Sele
ojdlhvh xedn veie sjs}kevh xedn imdmrhie |moeded xedn kmbh`
omrev eoedbmromibedn imdaelh sjs}kevh xedn rmvhv|md lekei ~eo|}
xedn kmbh` vhdnoe|
lhbedlhdnoed lmdned sjs}kevh xedn imdmrhie |moeded vmkmovh kmbh`
kmie`$
Sele leverdxe imoedhvim rmvhv|mdvh hdvmo|hvhle sele vmrednne
lese|
lhbenh imdaelh |hne |e`es$ Sele |e`es smr|eie |mraelh
smdhdnoe|ed
lm|jovhghoevh hdvmo|hvhle+ vm`hdnne hdvmo|hvhle imdaelh |hleo
bmref}d (`ek hdh
lhvmbeboed smdner}` omrae mdqhi |mr|md|}$ Omi}lhed |mraelh
smd}r}ded
omsmoeed |h|ho |ernm| hdvmo|hvhle sele |}b}`+$ \e`es vmkeda}|
|mraelh smd}r}ded
kea} smdm|revh hdvmo|hvhle imkek}h o}kh|+ vm`hdnne imdn`eibe|
iev}odxe
be`ed eo|hg hdvmo|hvhle led imdhdnoe|oed mdqhi
lm|jovhghoevh$
-
7/23/2019 uji bioassay & susceptibillity
9/20
8
BEB HHH
SRJVML]R SREO\HO
E$ ^eo|} Smkeovedeed@erh*\ednnek 1 Reb}* 58 Lmvmibmr 2352
^eo|} 1 30$63 ^HB
\mise| 1 Kebjre|jrh}i Rmoexeve
B$ Eke| led Be`ed]ah Bhjevvex5$ Eke| 1
e$ Nmkev skev|hob$ Keobed e|e} hvjkevhf$ V|js~e|f`
2$ Be`ed 1e$ Bmrbeneh iefei amdhv Hdvmo|hvhleb$ Dxei}of$
Omfje
]ah V}vfms|hbhkkh|x
5$ Eke| 1e$ \eb}dn fj}d| lerh shse SWF bmrvmoe| ihoeb$ Oehd
v|rhihdf$ Oesevl$ \hvv}mm$ Oerm| nmkedng$ Shsm|
-
7/23/2019 uji bioassay & susceptibillity
10/20
53
n$ Evshre|jr`$ Nmkev skev|hoh$ V|js~e|f`
2$ Be`ed 1e$ Hdvmf|hvhleb$ Keke|f$ Dxei}o
F$ Fere Omrae]ah Bhjevvex5$ Imdxhesoed eke| led be`ed xedn eoed
lhn}deoed2$ Imdxhesoed vmrednne xedn eoed lh}ah
e$ Dxei}o 15 Imdneibhk dxei}o lmdned evshre|jr+ = mojr dxei}o
}d|}o lhbmrh
smrkeo}ed led = mojr dxei}o }d|}o ojd|rjk (|edse smrkeo}ed
2
Imiev}ooeddxe omlekei nmkev skev|ho+ ve|} nmkev v}d|}o
fjd|rjkled ve|} nmkev xedn kehddxe }d|}o lhbmrh smrkeo}ed$
6 Imd}|}s nmkev skev|ho |mrvmb}| lmdned oehd v|rhihd led
oerm|nmkedn$
Imdnnmvmr nmkev skev|ho xedn eoed lhbmrh smrkeo}ed om
e|evsmri}oeed lhdlhdn xedn |mke` lhvmisrj|oed rmvhl}
hdvmo|hvhle$
-
7/23/2019 uji bioassay & susceptibillity
11/20
55
0 Imibheroed dxei}o ojd|eo lmdned rmvhl} hdvmo|hvhle
selesmri}oeed lhdlhdn vmkeie 63 imdh|$
8 Vm|mke` ~eo|} smdn}ahed vmkmveh+ imihdle`oed dxei}o
omlekeinmkev skev|ho bmr|}|}s oehd v|rhihd( imdn`h|}dn dxei}o
xedn
ie|h$
53Imibmrh ker}|ed n}ke 53- sele oesev vmbeneh d}|rhvh
dxei}o55Imdxhised dxei}o lekei oj|eo smdxhiseded vmkeie 2<
aei52Imdn`h|}dn omie|hed dxei}o sele smrkeo}ed led ojd|rjk
Ahoe omie|hed dxei}o sele smibedlhdn (ojd|rjk 1
e ; = -+ ieoe ednoe omie|hed lese| lhn}deoedb = -)23 -+ ieoe
omie|hed `er}v lhojrmovh lmdned r}i}v
Ebbjv 9
P 533 -
f 4 23 - omie|hed ojd|rjk }ah bhjevvex `er}v lh}kedn
b$ Omfje 15 Imdneibhk omfje lmdned ver}dn |edned+ vmbedxeo 6
mojr omfje$2 Imiev}ooeddxe omlekei nmkev skev|ho ievhdn)ievhdn 5
mojr
omfje smr nmkev skev|ho$
6 Imdneibhk bmbmrese sj|jdned keobed e|e} hvjkevh$ Imdfe|e|
smr}be`ed)smr}be`ed xedn |mraelh sele omfje xedn lh}ah$
-
7/23/2019 uji bioassay & susceptibillity
12/20
52
0 Imibedlhdnoed omfmse|ed lexe b}d}` |mr`eles omfje ed|ere
6amdhv hdvmo|hvhle xedn lhn}deoed$
]ah V}vfms|hbhkkh|x
5$ Imdxhesoed eke| led be`ed xedn eoed lhn}deoed2$ Imdxhesoed
vmrednne xedn eoed lh}ah
e$ Keke| 15 Imdneibhk keke| lmdned evshre|jr = mojr keke| }d|}o
lhbmrh
smrkeo}ed led = mojr keke| }d|}o fjd|rjk (|edse smrkeo}ed
2 Iev}ooed om lekei |eb}dn fj}d| smrkeo}ed xedn lh|}|}s
lmdnedoehd vr|hihd led lhbmrh oerm|
6 Keo}oed veie iev}ooed om lekei |eb}dn fj}d| |edse
smrkeo}edxedn lh|}|}s lmdned oehd vr|hihd led lhbmrh oerm|
Imdm|mvoed hdvmf|hvhle sele omr|ev |hvv}m sele |eb}dn
fj}d|smrkeo}ed
0 Imd}dnn} vmkeie 63 imdh| led eie|h bmrese a}ike` keke|
xednievh` `hl}s e|e} ie|h+ kh`e| a}ne sele fjd|rjk
8 Esebhke ele keke| xedn |hleo ie|h ieoe lh`jklhdn vmkeie 2<
aei+led omie|hed keke| lh e|ev 23- ieoe lese| lh iev}ooed om
lekei
r}i}v ebj|$
-
7/23/2019 uji bioassay & susceptibillity
13/20
56
b$ Dxei}o 15 Imdneibhk dxei}o lmdned evshre|jr = mojr dxei}o
}d|}o lhbmrh
smrkeo}ed led = mojr dxei}o }d|}o fjd|rjk (|edse smrkeo}ed
2 Iev}ooed om lekei |eb}dn fj}d| smrkeo}ed xedn lh|}|}s
lmdnedoehd vr|hihd led lhbmrh oerm|
6 Keo}oed veie iev}ooed om lekei |eb}dn fj}d| |edse
smrkeo}edxedn lh|}|}s lmdned oehd vr|hihd led lhbmrh oerm|
Imdm|mvoed hdvmf|hvhle sele omr|ev |hvv}m sele |eb}dn
fj}d|smrkeo}ed
0 Imd}dnn} vmkeie 63 imdh| led eie|h bmrese a}ike` keke|
xednievh` `hl}s e|e} ie|h+ kh`e| a}ne sele fjd|rjk
8 Esebhke ele dxei}o xedn |hleo ie|h ieoe lh`jklhdn vmkeie
25665553367$ Lmewh|e Hd|ed Sreledh SJ>56655533=>$ Lmvh Rhrhd
Djwh|e SJ>56655533>0$ Mdh Vegh|rhxedh SJ>5665553538$ Geaer
S}rdjij SJ>56655535253$ Gmdh \everh SJ>566555 3556655535752$
Hoe Er~emdh SJ>56655535056$ Kehke O`}vd}k O`e|hie`
SJ>56655532355665553225=$ I}`eiiel Edlx Ghriedvxe`
SJ>5665553256655532>5>$ Re|de L~h X}khd|hde
SJ>56655536350$ Vh|h D}raedde` SJ>56655536258$ \rh Djwh
V}ved|h SJ>56655536=23$ Xjkeibe Mrwhde V}aer~j SJ>566555
36>25$ X}khev|}|h SJ>566555368
