UCSD Lit Bldg Mold Report - University of California, San ... · 310 None Recent remodel (18 months), sink/kitchenette in area 254 None Sink in room, water staining on ceiling tile

Literature Building Mold Sampling Report

University of California, San Diego

Prepared By:

Aurora Industrial Hygiene

San Diego, CA

Prepared By: Date: April 6, 2009 Karen G. Shockley, CIH #6766

Literature Building Page 2 University of California, San Diego April 2009

TABLE OF CONTENTS

USE OF THIS REPORT ............................................................................................................................................. 3 1.0 Introduction ..................................................................................................................................................... 3 2.0 Executive Summary ......................................................................................................................................... 3 3.0 Observations ..................................................................................................................................................... 4

Table 1. Room Observations .............................................................................................................................. 4 4.0 Mold Sampling Methodology .......................................................................................................................... 5

Total Spore Air Samples ............................................................................................................................................ 6 Viable Air Samples .................................................................................................................................................... 6 Moisture Readings ..................................................................................................................................................... 6

5.0 Laboratory Results for Mold Sampling ......................................................................................................... 7 Table 2. Indoor and Outdoor Total Spore Air Sampling Results – March 5, 2009 ...................................... 8 Table 3. Indoor and Outdoor Viable Spore Air Sampling Results – March 5, 2009 .................................... 9 Table 4. Indoor Total Spore Air Sampling Results – 4th Floor ..................................................................... 10 Table 5. Indoor Total Spore Air Sampling Results – 3rd Floor ..................................................................... 11 Table 6. Indoor Total Spore Air Sampling Results – 2nd & 1st Floors .......................................................... 12 Table 7. Indoor Total Spore Air Sampling Results – 1st Floor...................................................................... 13 Table 8. Outdoor Total Spore Air Sampling Results – March 10 and 12, 2009 .......................................... 14

6.0 Mold Discussion ............................................................................................................................................. 15 7.0 Limitations ..................................................................................................................................................... 16 Appendix One – Photographs ................................................................................................................................... 17 Appendix Two – Site Diagrams ................................................................................................................................ 27 Appendix Three – Laboratory Reports of Analysis and Chains of Custody ........................................................ 31


USE OF THIS REPORT

This report is intended to provide an understanding of the potential hazards that the property evaluated in this report may pose to human health due to airborne mold. This report is based primarily upon data and information obtained during a three site visits by Aurora Industrial Hygiene, Inc. (Aurora) to the property identified herein on March 5, 10, and 12, 2009 and is based solely upon the condition of the property on the dates of such assessments.

Aurora has performed the work, made the findings, and proposed recommendations described in this report in accordance with generally accepted industrial hygiene and environmental science practices for mold in effect at the time the work was performed. This warrantee stands in lieu of all other warranties, expressed or implied. While this report can be used as a guide by the client, it must be understood that changing circumstances in the environment and in property usage can alter radically the conclusions and information contained in this report.

1.0 Introduction

This report documents the findings from mold sampling conducted by Aurora in the Literature Building (#627) on the UCSD La Jolla Campus in San Diego, California. The purposes of the survey were to visually assess the condition of the rooms sampled for signs of water damage or intrusion, and to conduct sampling to indicate whether mold was compromising the indoor air quality in selected rooms throughout all four floors of the building. The survey was limited to a list of rooms provided by UCSD personnel. Per the scope of work as outlined by UCSD, the remainder of the Literature Building was not assessed. In the course of completing this inspection, Aurora met with Steve Benedict and Sarah Meyer, UCSD Environment, Health and Safety Department; and Nancy Ho-Wu and Nancy Daly, Literature Department. Karen Shockley and Rick Shockley visited the site on March 5, 2009. Rick Shockley returned to the site on March 10 and 12, 2009 to complete the inspection and collect samples. All work was completed under the supervision of Certified Industrial Hygienist Karen Shockley (CIH #6766).

2.0 Executive Summary

Aurora was provided a list of rooms by UCSD. In each room, Aurora conducted a visual assessment and air sampling for mold. Moisture measurements were conducted if there was a visual indication of water intrusion and randomly of carpet and drywall walls in some of the rooms that were assessed. Air samples for total mold spores and viable mold spores were collected in the building and outdoors. The outdoor air samples were collected to provide an indication of background levels of airborne mold in the area. Surface tape lift samples for mold were not collected because no visible suspect mold was observed by Mr. Shockley.


Air sample results indicated that the airborne mold spores indoors were comparable to the outdoor/background sample locations. Results did not indicate any degradation of the air quality from mold. Moisture readings were all in the “safe/dry” range. 3.0 Observations

Aurora conducted a visual assessment of the rooms on the list provided by UCSD. Aurora conducted moisture measurements if there was a visual indication of water intrusion and randomly of carpet and drywall walls in some of the rooms that were assessed. The visual assessment included looking above drop ceiling tiles only if stained tiles were noted. The following table indicates the number of operable windows, whether they were opened or closed during the assessment, and any other notable observations found by Mr. Shockley. Surface tape lift samples for mold were not collected because visible suspect mold was not observed by Mr. Shockley.

Table 1. Room Observations

Room # Number of Operable Window –

Closed or Open Other Comments

454 None Office is dusty 453 1 – Slightly open 452 1 – Closed 451 1 – Closed 448 1 – Closed

445 1 – Open Could be a dog routinely in room, photo on shelf and

bowl on floor 443 1 – Closed 438 1 – Open Office is messy, dog food in room

434 1 – Closed Tea cup and honey on a table 431 1 – Closed 427 1 – Closed 425 1 – Closed Not used, pretty empty

410 None New office space

354 None Sink in the room 353 1 – Closed 349 1 – Closed 348 1 – Closed 346 1 – Closed

341 1 – Slightly open Office is messy, stain on carpet and wall near door

appear to be from coffee

337 None


Room # Number of Operable Window –

Closed or Open Other Comments

336 None 333 1 – Open 332 1 – Open 329 1 – Closed 328 1 – Closed 326 1 – Closed 324 1 – Closed

310 None Recent remodel (18 months), sink/kitchenette in area

254 None Sink in room, water staining on ceiling tile above sink

but everything looked dry above it 251 1 – Closed Fan on desk turned off prior to sampling 242 1 – Closed 240 1 – Slightly open 223 None

210 None There is a kitchen area with microwave and there was a

food odor during sampling 110 None 116 1 – Closed 117 1 – Closed 123 1 – Closed Dirty on top of filing cabinets 124 1 – Closed Computer server room 128 1 – Closed 129 1 – Closed 130 1 – Closed 133 1 – Closed 134 1 – Closed 137 Double door – Closed Sink in room 138 Double door – Open Refrigerator and microwave in room 155 2 Double doors – Closed 139 1 – Closed 140 1 – Closed 141 1 – Closed 150 1 – Closed

4.0 Mold Sampling Methodology On March 5, 2009 Mr. Shockley collected air samples from ten sample locations, eight inside the building (two on each floor) and two outside of the building. On March 10, 2009 Mr. Shockley collected air samples from 45 sample locations, 42 inside the building (several on each floor) and


three outside of the building. On March 12, 2009 Mr. Shockley collected air samples from two sample locations, one inside room 242, which was inaccessible on March 10, 2009, and one outside of the building. The outdoor air samples were collected to provide an indication of background levels of airborne mold in the area. At each of the sample locations on March 5, one air sample was collected to assess total airborne spores and one was collected to assess viable airborne spores. On March 10 and 12, only air sampling for total mold spores was conducted. Surface tape lift samples for mold were not collected because visible suspect mold was not observed by Mr. Shockley. Moisture measurements were collected randomly of carpet and drywall walls in some of the rooms that were assessed and all of the readings were in the “Safe/Dry” range. Total Spore Air Samples

Air-O-Cell spore trap 37-mm cassettes are used to assess total spores. A sampling pump calibrated to a flow rate of 15 liters per minute draws a known volume of air over a sampling period of up to five (5) minutes. Once collected, the sample is submitted to a microbial laboratory where a small glass slide is removed from the cassette for direct examination. Viable Air Samples

Air sampling for viable airborne mold spores and bacteria is performed using the Aerotech A-6 Single Stage Microbial Sampler, through which a known volume of air is collected and recorded. A sampling pump calibrated to a flow rate of 28.3 liters per minute is used to draw air through the A-6 sampling device. Within the A-6 sampling device is a petri dish that contains a laboratory prepared agar gel. Airborne particulates are deposited onto the agar gel. The sample is then sealed and submitted to a microbial laboratory where it is incubated in a controlled environment for 7-14 days. The petri dish samples are then assessed for viable mold and/or bacteria colonies, and reported in colony forming units, or CFUs. All of the collected mold samples were assigned a unique sample identification number, placed in sealed containers, labeled, and hand-delivered by Aurora Industrial Hygiene personnel to EMLab P&K Laboratory (EMLab), located at 9089 Clairemont Mesa Blvd, Suite 106, San Diego, CA 92123. EMLab is accredited by the American Industrial Hygiene Association for microbiological analyses. Moisture Readings

Moisture readings were collected using a Protometer penetrating moisture meter. In wood, the instrument measures the material’s actual percent moisture content (%H2O). When testing material other than wood, the meter measures the wood moisture equivalent (WME) value of the material. WME is the moisture level that would be attained by a piece of wood in equilibrium with the material being tested. As the critical moisture levels for wood are known, WME measurements enable the moisture meter user to establish if materials are in a safe air dry, borderline or damp condition.

<18% (Green Zone) The material is in a Safe Dry condition, moisture related problems of decay/deterioration should not occur.


18 – 20% (Yellow Zone) The material is in a Borderline condition, decay/deterioration may occur under certain conditions.

>20% (Red Zone) The material is in a Wet condition, decay/deterioration is inevitable in time unless the moisture level of the material is reduced.

5.0 Laboratory Results for Mold Sampling Tables 2, 3, 4, 5, 6, 7 and 8 summarize the laboratory results for mold air sampling. Photographs of some of the sample locations are included in Appendix One, a sample location diagram is included in Appendix Two and laboratory reports of analysis are included in Appendix Three.


Table 2. Indoor and Outdoor Total Spore Air Sampling Results – March 5, 2009

Sample Identification

0305-01A Outdoors,

West of Building

0305-02A Room 134

0305-03A Room 110

0305-04A Room 223

0305-05A Room 254

0305-06A Room 310

0305-07A Room 349

0305-08A Room 427

0305-09A Room 438

0305-10A Outdoors, South of Building

Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3

Alternaria 13

Ascospores 80 53 13

Basidiospores 93 13 53 13 13 53

Cladosporium 53 53 53 160 110

Other brown 13 13 13 13 13 13 13 27

Penicillium/ Aspergillus types

53 53

Pithomyces 13

Rusts 13

Smuts, Periconia, Myxomycetes

13 27 13 13

Ulocladium

Background Debris1 1+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 1+

Sample Volume(liters)

75 75 75 75 75 75 75 75 75 75

Spores/ m3 250 120 13 120 80 80 13 250 40 170

1 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported.


Table 3. Indoor and Outdoor Viable Spore Air Sampling Results – March 5, 2009


0305-01B Outdoors,

West of Building

0305-02B Room 134

0305-03B Room 110

0305-04B Room 223

0305-05B Room 254

0305-06B Room 310

0305-07B Room 349

0305-08B Room 427

0305-09B Room 438

0305-10B Outdoors, South of Building

Fungi CFU2/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3

Aureobasidium 12

Cladosporium 47 24 12 82 12 24 24 130

Non-sporulating fungi

12 12 12

Penicillium 24 24 12 12 59

Yeasts 12


84.9 84.9 84.9 84.9 84.9 84.9 84.9 84.9 84.9 84.9

CFU/ m3 82 24 24 120 12 <12 <12 35 59 190

2 CFU = colony forming units


Table 4. Indoor Total Spore Air Sampling Results – 4th Floor

Sample Identification 0310-02 Room

454

0310-03 Room

453

0310-04 Room

452

0310-05 Room

451

0310-06 Room

448

0310-07 Room

445

0310-08 Room

443

0310-09 Room

434

0310-10 Room

431

0310-11 Room

425

0310-12 Room

410

Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3

Alternaria 13

Ascospores

Basidiospores 13 13 27 40

Cladosporium 53 53 53 53 53 53 53 110

Other brown 27 13


53 53 110 53

Pithomyces

Rusts 13


13

Ulocladium

Background Debris3

2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+


75 75 75 75 75 75 75 75 75 75 75

Spores/ m3 53 120 67 80 53 80 200 <13 110 160 <13



Table 5. Indoor Total Spore Air Sampling Results – 3rd Floor


0310-13 Room

354

0310-14 Room

353

0310-15 Room

348

0310-16 Room

346

0310-17 Room

341

0310-18 Room

337

0310-19 Room

336

0310-20 Room

333

0310-21 Room

332

0310-22 Room

329

0310-23 Room

328

0310-24 Room

326

0310-25 Room

324

Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3

Alternaria 13 13 13 Basidiospores 13 13 13 13 Bipolaris/ Drechlera group

13 53

Cladosporium 53 53 160 53 53 Other brown 27 13 27 13 27 Penicillium/ Aspergillus types

53

Rusts 13 Smuts, Periconia, Myxomycetes

13 27 13

Stachybotrys 13 Torula 13 Trichocladium 13 13 Background Debris4 2+ 2+ 2+ 3+ 2+ 2+ 2+ 2+ 2+ 2+ 3+ 2+ 2+

Sample Volume (liters)

75 75 75 75 75 75 75 75 75 75 75 75 75

Spores/ m3 27 93 130 190 110 27 13 27 67 27 80 13 13



Table 6. Indoor Total Spore Air Sampling Results – 2nd & 1st Floors


251

0310-27 Room

240

0312-01 Room

242

0310-28 Room

210

0310-30 Room

116

0310-31 Room

117

0310-32 Room

123

0310-33 Room

124

0310-34 Room

128

Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3

Alternaria 13

Ascospores

Basidiospores 40 80 13

Bipolaris/ Drechlera group 13

Cladosporium 53 110 320 53 110 160 53

Epicoccum 13 13

Other brown 13 13


110 53 110 53 53

Rusts


13 13 13

Ulocladium

Background Debris5 2+ 3+ 3+ 2+ 3+ 2+ 3+ 2+ 2+

Sample Volume(liters) 75 75 75 75 75 75 75 75 75

Spores/ m3 110 320 350 110 230 53 190 67 67



Table 7. Indoor Total Spore Air Sampling Results – 1st Floor


129

0310-36 Room

130

0310-37 Room

133

0310-38 Room

137

0310-39 Room

138

0310-40 Room

155

0310-41 Room

139

0310-42 Room

140

0310-43 Room

141

0310-44 Room

150

Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3

Alternaria

Ascospores 13

Basidiospores 13 13 13

Cladosporium 53 53 53

Curvularia 13

Epicoccum 13

Other brown 13 13 13 13


53 53 53 53 53

Rusts


13

Ulocladium

Background Debris6 2+ 2+ 2+ 2+ 2+ 2+ 2+ 3+ 2+ 2+

Sample Volume(liters) 75 75 75 75 75 75 75 75 75 75

Spores/ m3 <13 67 110 130 27 13 67 130 <13 27



Table 8. Outdoor Total Spore Air Sampling Results – March 10 and 12, 2009


0310-01 Outdoors,

East of building

0310-29 Outdoors,

West of building

0310-45 Outdoors, South of building

0312-02 Outdoors, South of building

Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3

Alternaria 13

Ascospores

Basidiospores 53 93 40 67

Bipolaris/ Drechlera group

Cladosporium 110 53 160 53

Curvularia

Epicoccum

Other brown 27


450 53

Pithomyces

Rusts


Stachybotrys

Torula

Trichocladium

Ulocladium

Background Debris7 2+ 2+ 2+ 1+

Sample Volume(liters) 75 75 75 75

Spores/ m3 160 640 250 120



6.0 Mold Discussion No regulatory standards exist for air or surface mold spores. High variability in mold spore concentrations will exist in different geographic locations, seasons, local weather patterns, and on a diurnal basis. The following basic guide provided by ETA Laboratories was used as a reference. Typical Indoor Mold Spore Concentration Ranges

Description Spores /m3 Predominant Types "Clean" building Less than 2,000

Less than 700 Less than 500

Total for all spore types Penicillium, Aspergillus "Outdoor spores": alternaria, ascospores, basidiospores, cladosporium

Possible Indoor Amplification 1,000 - 5,000 Penicillium, Aspergillus, Cladosporium

Indoor amplification present 5,000 - 10,000 Penicillium, Aspergillus, Cladosporium

Chronic Indoor Amplification 10,000 - 6,000,000

Penicillium, Aspergillus, Cladosporium, Stachybotrys, Basidiospores

As a general rule, indoor fungal problems are usually indicated when a significant difference is demonstrated between indoor and outdoor airborne spore concentrations or types of spore genera or species. Mold spores concentrations indoors were comparable to outdoor samples and results did not indicate an indoor amplification of mold. Surface tape lift samples for mold were not collected because visible suspect mold was not observed by Mr. Shockley. Moisture measurements were collected randomly of carpet and drywall walls in some of the rooms that were inspected and all of the readings were in the “Safe/Dry” range.


7.0 Limitations The data and observations collected during the course of this assessment have been gathered to provide the Client with information pertaining to the areas of the subject property identified in this report. Although Aurora believes that the findings and conclusions provided in this report are reasonable, the assessment is limited to the conditions observed and to the information available at the time of the work. Due to the nature of the work, there is a possibility that there may exist conditions which could not be identified within the scope of the assessment or which were not apparent at the time of our site work. The assessment is also limited to information available from the client at the time it was conducted. It is also possible that the testing methods employed at the time of the report may later be superceded by other methods. Aurora does not accept responsibility for changes in the state of the art.

Aurora does not guarantee that all mold contaminated areas in the subject property were recognized during our evaluation. This report is limited to the samples taken and locations sampled. Additional sampling may be needed to further identify other pollutants or other mold contaminated areas at the subject property. Microbial growth may occur if sources of moisture are not remediated.

We hope that this information is helpful. Please feel free to contact us at (619) 276-5901 if you have any questions.


Appendix One – Photographs

Photo One – Satellite photo. The dashed box is around the section of the UCSD La Jolla Campus that includes the

Literature Building.

Interstate 5

Interstate 805


Photo Two – Another satellite photo. The red arrow indicates the Literature Building.

Interstate 5

Genesee Avenue


Photo Three – Close-up photo of the Literature Building. The locations of the outdoor samples are indicated.

Sample location west of the building

Sample location south of the building

Sample location east of the building


Photo Four – Outdoor sample location east of the building.

Photo Five – Outdoor sample location south of the building.


Photo Six – Outdoor sample location west of the building.

Photo Seven – Samples 0305-09A and 09B collected in room 438. The red arrow points towards a water dish and

cup of dog food found in the room.


Photo Eight – Sample 0310-08 collected in room 434. The red arrow points to the tea cup and honey on the table.

Photo Nine – Samples 0305-06A and 06B collected in room 310.


Photo Ten – Sample 0310-17 collected in room 341.

Photo Eleven – Staining on carpet and wall in room 341 that appear to be from coffee.


Photo Twelve – Samples 0305-05A and 05B collected in room 254.

Photo Thirteen – Staining on ceiling tile in room 254, everything looked dry above the stain.


Photo Fourteen – Sample 0310-28 collected in room 210.

Photo Fifteen – Samples 0305-02A and 02B collected in room 134.


Photo Sixteen – Sample 0310-32 collected in room 123.


Appendix Two – Site Diagrams DIAGRAM NOT TO SCALE

0305-01

0310-29

0305-10

0310-45

0305-03

0310-01

0305-02

0310-30

0312-02

0310-31

0310-32

0310-33

0310-34

0310-35

0310-36 0310-37

0310-38

0310-39

0310-40

0310-44

0310-41

0310-42

0310-43


0305-04 0310-28

0305-05

0310-26 0310-27

0312-01


0305-07

0310-15

0305-06

0310-17

0310-16

0310-18

0310-13

0310-14

0310-19

0310-20

0310-21

0310-22 0310-23

0310-24

0310-25


0305-08

0310-02

0305-09

0310-03

0310-04

0310-05

0310-06

0310-07

0310-08

0310-09 0310-10

0310-11 0310-12


Appendix Three – Laboratory Reports of Analysis and Chains of Custody

EMLab P&K

Approved by:

Lab ManagerMalcolm Moody

Report for:

Ms. Karen ShockleyAurora Industrial Hygiene, Inc.1094 Cudahy PlaceSuite 120San Diego, CA 92110

Regarding: Project: UCSD-Literature BuildingEML ID: 519266

EMLab P&K

This coversheet is included with your report in order to comply with AIHA and ISO accreditation requirements.

For clarity, we report the number of significant digits as calculated; but, due to the nature of this type of biological data, the number of significant digits that is used for interpretation should generally be one or two. All samples were received in acceptable condition unless noted in the Report Comments portion in the body of the report. Due to the nature of the analyses performed, field blank corrections of results is not a standard practice. The results relate only to the items tested.

EMLab P&K ("the Company") shall have no liability to the client or the client's customer with respect to decisions or recommendations made, actions taken or courses of conduct implemented by either the client or the client's customer as a result of or based upon the Test Results. In no event shall the Company be liable to the client with respect to the Test Results except for the Company's own willful misconduct or gross negligence nor shall the Company be liable for incidental or consequential damages or lost profits or revenues to the fullest extent such liability may be disclaimed by law, even if the Company has been advised of the possibility of such damages, lost profits or lost revenues. In no event shall the Company's liability with respect to the Test Results exceed the amount paid to the Company by the client therefor.

Document Number: 200091 - Revision Number: 5

Dates of Analysis:Culturable air fungi (Incl. Asp spp.): 03-12-2009Spore trap analysis: 03-09-2009

Project SOPs: Culturable air fungi (Incl. Asp spp.) (I100002), Spore trap analysis (I100000)

EMLab P&K1010 N Central Avenue, Glendale, CA 91202

(866) 465-6653 Fax (858) 569-5806 www.emlab.comClient: Aurora Industrial Hygiene, Inc.C/O: Ms. Karen ShockleyRe: UCSD-Literature Building

Date of Sampling: 03-05-2009Date of Receipt: 03-05-2009Date of Report: 03-09-2009

SPORE TRAP REPORT: NON-VIABLE METHODOLOGYLocation: 0305-01A:

Outdoors, near main

entrance

0305-02A:Room 134

0305-03A:Room 110

0305-04A:Room 223

Comments (see below) None None None None

Lab ID-Version‡: 2298722-1 2298723-1 2298724-1 2298725-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores* 3 80AureobasidiumBasidiospores* 4 93 1 13 1 53Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 1 13 1 13 1 13 1 13Other colorlessPenicillium/Aspergillus types† 1 53Pithomyces 1 13Rusts*Smuts*, Periconia, Myxomycetes* 1 13 2 27StachybotrysStemphyliumTorulaUlocladiumZygomycetesBackground debris (1-4+)†† 1+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 250 120 13 120Comments:

EMLab ID: 519266, Page 1 of 3TestAmerica Environmental Microbiology Laboratory, Inc.

* Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens.† The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted.††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels.The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume.‡ A "Version" greater than 1 indicates amended data.§ Total Spores/m3 has been rounded to two significant figures to reflect analytical precision.





Room 2540305-06A:Room 310

0305-07A:Room 349

0305-08A:Room 427


Lab ID-Version‡: 2298726-1 2298727-1 2298728-1 2298729-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3Alternaria 1 13ArthriniumAscospores* 1 53AureobasidiumBasidiospores* 1 13Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53 3 160CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 1 13 1 13 1 13Other colorlessPenicillium/Aspergillus types† 1 53PithomycesRusts* 1 13Smuts*, Periconia, Myxomycetes* 1 13 1 13StachybotrysStemphyliumTorulaUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 80 80 13 250Comments:







Room 4280305-10A:

Outdoors, near south end of bldgComments (see below) None None

Lab ID-Version‡: 2298730-1 2298731-1

raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores* 1 13AureobasidiumBasidiospores* 1 13 1 53Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 2 110CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 2 27Other colorlessPenicillium/Aspergillus types†PithomycesRusts*Smuts*, Periconia, Myxomycetes*StachybotrysStemphyliumTorulaUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 1+Sample volume (liters) 75 75§ TOTAL SPORE/m3 40 170Comments:



EMLab P&K1150 Bayhill Drive, Suite 100, San Bruno, CA 94066



CULTURABLE AIR FUNGI REPORTLocation: 0305-01B:

Outdoors, near main

entrance

0305-02B:Room 134

0305-03B:Room 110

0305-04B:Room 223


Lab ID-Version‡: 2298712-1 2298713-1 2298714-1 2298715-1

raw ct. cfu*/m3 raw ct. cfu*/m3 raw ct. cfu*/m3 raw ct. cfu*/m3AcremoniumAlternariaAspergillus flavusAspergillus fumigatusAspergillus nidulansAspergillus nigerAspergillus ochraceusAspergillus versicolorAureobasidiumBasidiomycetesBipolaris/Drechslera groupBotrytisChaetomiumCladosporium 4 47 2 24 1 12 7 82CurvulariaEpicoccumFusariumNon-sporulating fungi 1 12 1 12PaecilomycesPenicillium 2 24 2 24PhomaRhizopusStachybotrys chartarumUlocladiumYeasts 1 12Positive Hole 400 400 400 400Sample volume (liters) 84.9 84.9 84.9 84.9§ TOTAL CFU*/M3 82 24 24 120* cfu = colony forming units Positive hole correction chart used for all calculationsComments:


Note: Interpretation is left to the company and/or persons who conducted the field work. Variation is an inherent part of biological sampling. The presence or absence of a few genera in small numbers should not be considered abnormal.NORMAL SPORE LEVELS: Indoor spore levels usually average 30 to 80% of the outdoor spore level at the time of sampling, with the same general distribution of spore types. Filtered air, air-conditioned air, or air remote from outside sources may average 5 to 15% of the outside airat the time of sampling. (These percentages are guidelines, only. A major factor is the accessibility of outdoor air. A residence with open doors and windows and heavy foot traffic may average 95% of the outdoor level while high rise office buildings with little air exchange may average 2%. Dusty interiors may exceed 100% of the outdoors to some degree, but will still mirror the outdoor distribution of spore types.)PROBLEM INTERIORS: A substantial increase of one or two spore types which are inconsistent with and non-reflective of the outside distribution of spore types is usually indicative of an indoor reservoir of mold growth.The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume.‡ A "Version" greater than 1 indicates amended data.§ Total CFU/m3 has been rounded to two significant figures to reflect analytical precision.





Room 2540305-06B:Room 310

0305-07B:Room 349

0305-08B:Room 427


Lab ID-Version‡: 2298716-1 2298717-1 2298718-1 2298719-1

raw ct. cfu*/m3 raw ct. cfu*/m3 raw ct. cfu*/m3 raw ct. cfu*/m3AcremoniumAlternariaAspergillus flavusAspergillus fumigatusAspergillus nidulansAspergillus nigerAspergillus ochraceusAspergillus versicolorAureobasidiumBasidiomycetesBipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 12 2 24CurvulariaEpicoccumFusariumNon-sporulating fungiPaecilomycesPenicillium 1 12PhomaRhizopusStachybotrys chartarumUlocladiumYeastsPositive Hole 400 400 400 400Sample volume (liters) 84.9 84.9 84.9 84.9§ TOTAL CFU*/M3 12 < 12 < 12 35* cfu = colony forming units Positive hole correction chart used for all calculationsComments:







Room 4280305-10B:

Outdoors, near south end of bldgComments (see below) None None

Lab ID-Version‡: 2298720-1 2298721-1

raw ct. cfu*/m3 raw ct. cfu*/m3AcremoniumAlternariaAspergillus flavusAspergillus fumigatusAspergillus nidulansAspergillus nigerAspergillus ochraceusAspergillus versicolorAureobasidium 1 12BasidiomycetesBipolaris/Drechslera groupBotrytisChaetomiumCladosporium 2 24 11 130CurvulariaEpicoccumFusariumNon-sporulating fungi 1 12PaecilomycesPenicillium 1 12 5 59PhomaRhizopusStachybotrys chartarumUlocladiumYeastsPositive Hole 400 400Sample volume (liters) 84.9 84.9§ TOTAL CFU*/M3 59 190* cfu = colony forming units Positive hole correction chart used for all calculationsComments:



EMLab P&K

Approved by:

Lab ManagerMalcolm Moody

Report for:


Regarding: Project: UCSD-Literature BldgEML ID: 520993

EMLab P&K





Dates of Analysis:Spore trap analysis: 03-13-2009

Project SOPs: Spore trap analysis (I100000)


(866) 465-6653 Fax (858) 569-5806 www.emlab.comClient: Aurora Industrial Hygiene, Inc.C/O: Ms. Karen ShockleyRe: UCSD-Literature Bldg


SPORE TRAP REPORT: NON-VIABLE METHODOLOGYLocation: 0310-01:

Outdoors, east of bldg

0310-02:Room 454

0310-03:Room 453

0310-04:Room 452

0310-05:Room 451

Comments (see below) None None None None None

Lab ID-Version‡: 2306287-1 2306288-1 2306289-1 2306290-1 2306291-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3

Alternaria 1 13ArthriniumAscospores*AureobasidiumBasidiospores* 1 53 1 13Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 2 110 1 53 1 53 1 53 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 2 27Other colorlessPenicillium/Aspergillus types† 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes*StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75 75§ TOTAL SPORE/m3 160 53 120 67 80Comments:







Room 4480310-07:

Room 4450310-08:

Room 4430310-09:

Room 434Comments (see below) None None None None

Lab ID-Version‡: 2306292-1 2306293-1 2306294-1 2306295-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores*AureobasidiumBasidiospores* 1 13 2 27Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brownOther colorlessPenicillium/Aspergillus types† 1 53 2 110PithomycesRusts* 1 13Smuts*, Periconia, Myxomycetes* 1 13StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 53 80 200 < 13Comments:







Room 4310310-11:

Room 4250310-12:

Room 4100310-13:


Lab ID-Version‡: 2306296-1 2306297-1 2306298-1 2306299-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3Alternaria 1 13ArthriniumAscospores*AureobasidiumBasidiospores* 3 40Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53 2 110CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 1 13Other colorlessPenicillium/Aspergillus types† 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes* 1 13StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 110 160 < 13 27Comments:







Room 3530310-15:

Room 3480310-16:

Room 3460310-17:


Lab ID-Version‡: 2306300-1 2306301-1 2306302-1 2306303-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3Alternaria 1 13ArthriniumAscospores*AureobasidiumBasidiospores* 1 13 1 13Bipolaris/Drechslera group 1 13BotrytisChaetomiumCladosporium 1 53 1 53 3 160 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 2 27 1 13 2 27Other colorlessPenicillium/Aspergillus types† 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes* 2 27StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 3+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 93 130 190 110Comments:







Room 3370310-19:

Room 3360310-20:

Room 3330310-21:


Lab ID-Version‡: 2306304-1 2306305-1 2306306-1 2306307-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3Alternaria 1 13ArthriniumAscospores*AureobasidiumBasidiospores* 1 13Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 1 13Other colorlessPenicillium/Aspergillus types†PithomycesRusts* 1 13Smuts*, Periconia, Myxomycetes*Stachybotrys 1 13StemphyliumTorulaTrichocladium 1 13UlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 27 13 27 67Comments:







Room 3290310-23:

Room 3280310-24:

Room 3260310-25:


Lab ID-Version‡: 2306308-1 2306309-1 2306310-1 2306311-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores*AureobasidiumBasidiospores* 1 13Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 2 27Other colorlessPenicillium/Aspergillus types†PithomycesRusts*Smuts*, Periconia, Myxomycetes* 1 13StachybotrysStemphyliumTorula 1 13Trichocladium 1 13UlocladiumZygomycetesBackground debris (1-4+)†† 2+ 3+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 27 80 13 13Comments:







Room 2510310-27:

Room 2400310-28:

Room 2100310-29:

Outdoors, west of building

Comments (see below) None None None A

Lab ID-Version‡: 2306312-1 2306313-1 2306314-1 2306315-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3Alternaria 1 13ArthriniumAscospores*AureobasidiumBasidiospores* 3 40 3 80 4 93Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53 2 110 1 53 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 1 13 2 27Other colorlessPenicillium/Aspergillus types† 2 110 1 53 31 450PithomycesRusts*Smuts*, Periconia, Myxomycetes* 1 13 1 13StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 3+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 110 320 110 640Comments:A) 30 of the raw count Penicillium/Aspergillus type spores were present as a single clump.







Room 1160310-31:

Room 1170310-32:

Room 1230310-33:


Lab ID-Version‡: 2306316-1 2306317-1 2306318-1 2306319-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores*AureobasidiumBasidiospores*Bipolaris/Drechslera group 1 13BotrytisChaetomiumCladosporium 2 110 3 160 1 53CurvulariaEpicoccum 1 13FusariumMyrotheciumNigrosporaOther brown 1 13Other colorlessPenicillium/Aspergillus types† 2 110 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes* 1 13StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 3+ 2+ 3+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 230 53 190 67Comments:







Room 1280310-35:

Room 1290310-36:

Room 1300310-37:


Lab ID-Version‡: 2306320-1 2306321-1 2306322-1 2306323-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores*AureobasidiumBasidiospores*Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53CurvulariaEpicoccum 1 13FusariumMyrotheciumNigrosporaOther brownOther colorlessPenicillium/Aspergillus types† 1 53 1 53 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes* 1 13StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 67 < 13 67 110Comments:







Room 1370310-39:

Room 1380310-40:

Room 1550310-41:


Lab ID-Version‡: 2306324-1 2306325-1 2306326-1 2306327-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores* 1 13AureobasidiumBasidiospores* 1 13 1 13 1 13Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther brown 1 13 1 13Other colorlessPenicillium/Aspergillus types† 1 53 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes*StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 2+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 130 27 13 67Comments:







Room 1400310-43:

Room 1410310-44:

Room 1500310-45:

Outdoors, near SW corner


Lab ID-Version‡: 2306328-1 2306329-1 2306330-1 2306331-1

raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3AlternariaArthriniumAscospores*AureobasidiumBasidiospores* 3 40Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 1 53 3 160Curvularia 1 13Epicoccum 1 13FusariumMyrotheciumNigrosporaOther brown 1 13 1 13Other colorlessPenicillium/Aspergillus types† 1 53 1 53PithomycesRusts*Smuts*, Periconia, Myxomycetes*StachybotrysStemphyliumTorulaTrichocladiumUlocladiumZygomycetesBackground debris (1-4+)†† 3+ 2+ 2+ 2+Sample volume (liters) 75 75 75 75§ TOTAL SPORE/m3 130 < 13 27 250Comments:



EMLab P&K

Approved by:

Lab ManagerMagzoub Ismail

Report for:


Regarding: Project: UCSD-Literature BuildingEML ID: 521481

EMLab P&K





Dates of Analysis:Spore trap analysis: 03-16-2009

Project SOPs: Spore trap analysis (I100000)

EMLab P&K9089 Clairemont Mesa Blvd, Suite 106, San Diego, CA 92123




Room 2420312-02:

Outdoors, near SW cornerComments (see below) None None

Lab ID-Version‡: 2308752-1 2308753-1

raw ct. spores/m3 raw ct. spores/m3Alternaria 1 13ArthriniumAscospores*AureobasidiumBasidiospores* 1 13 2 67Bipolaris/Drechslera groupBotrytisChaetomiumCladosporium 6 320 1 53CurvulariaEpicoccumFusariumMyrotheciumNigrosporaOther colorlessPenicillium/Aspergillus types†PithomycesRusts*Smuts*, Periconia, Myxomycetes*StachybotrysStemphyliumTorulaUlocladiumZygomycetesBackground debris (1-4+)†† 3+ 1+Sample volume (liters) 75 75§ TOTAL SPORE/m3 350 120Comments:



Documents

UCSD Lit Bldg Mold Report - University of California, San ... · 310 None Recent remodel (18 months), sink/kitchenette in area 254 None Sink in room, water staining on ceiling tile