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Translation
tRNA
• The process of assembling a protein from
the genetic information on an mRNA
molecule
“cooking from a recipe”
“building from instructions”
• Transfer RNA
• “t” shaped molecule that links each codon
on the mRNA molecule to its specific amino
acid
Amino Acid
Attachment
Site
Anti – Codon
Arm
Anti – Codon:
3 base pair sequence that is
complimentary to a specific
codon on an mRNA
Amino – Acyl Arm
Ribosomes • A specialized structure that “reads” the
mRNA and co – ordinates the action of the
tRNA and the assembly of proteins
Have 2 subunits:
1. Small Subunit – reads mRNA
2. Large Subunit – handles tRNA
– The large subunit has 3 binding sites for tRNA:
– A Site (Aminoacyl tRNA Binding Site)
– P Site (Peptidyl tRNA Binding Site)
– E Site (Exit Site)
The Process
The Process:
Step #1
Initiation
• mRNA binds to an active Ribosome
• Binds in a way that 2 neighbouring codons
are exposed
• Ribosome reads the mRNA in the 5’ to 3’
direction
• Ribosome reads the initiator codon (AUG)
and the initiator tRNA carrying the amino
acid methionine binds to the A – Site of
the Ribosome
Step #2:
Elongation
• The tRNA bound to the A – Site of the
ribosome slides into the P –Site as the
ribosome moves along the mRNA
• A new tRNA carrying a new amino acid
corresponding to the codon exposed in the
A – site binds to the ribosome
• A peptide bond forms between the new
amino acid and the amino acid attached to
the tRNA in the P – Site forming a larger
polypeptide chain
• The tRNA in the P – Site slides into the
E –Site where it is ejected and the process
repeats.
Step #3:
Termination
• Elongation continues until the ribosome
reads a “stop” codon on the mRNA
• When a stop codon is encountered a
“release” factor binds to the termination
codon and the complete polypeptide chain is
released
• The chain is then “processed” into a
functional protein