TRAINING WORKSHOP PROGRAMS

TRAINING WORKSHOP PROGRAMS

Helpline: 09891179928 [email protected] of 1 19

mailto:[email protected]

Recognising the need of trained human resource in the field of Biotechnology, Life Sciences ,Chemistry ,Pharmacy, Forensic Science, Food Technology and applied sciences . Allele Life Sciences is conducting several skill development programs to make youth employable.

IMPORTANT NOTES :

• Industry Oriented, Interdisciplinary Approach Hands on Learning Programs • State of art lab facility for hands on experience • As a part of our training program, we may suggest your name to our clients against

available vacancy • This training is limited to skill development only and we do not assure you for employment

or campus interview • Our prime motive is “Bridging Gap Between Industry & Academia” • All units are compulsory for certificate after completion

DURATION OF TRAINING PROGRAM: 40 Hrs or 1 to 2 Weeks

TRAINING FEE FOR INDIAN NATIONAL : INR 6,000 / ( For Single Workshop )

WHO MAY APPLY : BSc / B.Tech / MSc / MTech in any Chemistry / Forensic Science / Pharmacy Life Sciences / Biotechnology studying or completed students

AVAILABLE TRAINING PROGRAM :

NAME OF TRAINING PROGRAM Page No.

1 Training Workshop on PCR & Real Time PCR Technology 3

2 Training Workshop on Recombinant DNA Technology 4

3 Training Workshop on DNA Methylation Studies 5

4 Training Workshop on Protein Purification Strategies 6

5 Training Workshop on Amino Acid Analysis 7

6 Training Workshop on Protein Characterisation Techniques 8

7 Training Workshop on Adultartion in Food Products 9

8 Training Workshop on Quality Analysis of Alcoholic Beverages 10

9 Training Workshop on Role of DNA in Forensics 11

10 Training Workshop on Toxicology & Medico-Legal Evidence 12

11 Training Workshop on Bio-Separation by HPLC 13

12 Training in Workshop on Bio-Separation by Gas Chromatography 14



PROGRAM 1 : TRAINING WORKSHOP ON PCR & REAL TIME PCR TECHNOLOGY

It is a technique used for Gene expression analysis ; Cancer research, Drug research Disease diagnosis and management; Viral quantification Food testing ; GMO food, Animal and plant breeding; and many more life science applications.

COURSE CONTENT

UNIT I : INTRODUCTION TO PCR TECHNOLOGY, NUCLEIC ACID EXTRACTION & PRIMERS

Nucleic Acid Extraction , Quality Control Topics include: Extraction of Nucleic Acid , Gel Electrophoresis , Quantitative and Qualitative Analysis of Samples , Troubleshooting of Nucleic Acid Extraction

Primer Designing & Selection Topics include: Primer designing for cloning & Expression , Primer Selection for Molecular Marker Analysis

UNIT II : PCR CONDITION OPTIMISATION AND RUN FOR THE SAMPLE

PCR Optimisation Topics include: GC contents in Primers , Role of annealing temperature , Role of Contamination , Role of PCR Cycles etc. Troubleshooting Topics include: Order of troubleshooting, Examples of common problems and causes, Practical examples.

PCR Run for Reactions Topics include: Run of PCR reactions for biological samples Module Seven – Analysis of PCR Results Topics include: Analysis of results

UNIT III : INTRODUCTION TO REAL TIME PCR & TROUBLESHOOTING

Introduction to Real-time PCR & Applications: Fluorescence Principles, Absorption and emission of fluorophores ; Fluorescence resonance energy transfer (FRET), DNA intercalating dyes, and Probe based detection strategies. Troubleshooting of real-time PCR reactions: Data analysis of real-time PCR reactions: Step-for-step analysis, Absolute quantification, Relative quantification, Allelic discrimination, Plus/Minus assays.

UNIT III : REAL TIME PCR REACTION SETUP , SAMPLE RUN & DATA ANALYSIS

Real-time PCR reaction setup: Construction of a standard curve: Biostatistics principles; Linear regression , Standard melt curve analysis ,High resolution melt curve analysis Practical Session II: Hands-on real-time PCR experience,Delegates set up their own SYBR Green real-time PCR reactions, Real-time PCR optimisation

Analysis of SYBR Green real-time PCR results: Melt curve analysis , Compare results from practical sessions I and II , Identify factors affecting success of real-time PCR reactions: Primer dimers; GLP aspects . Amplification efficiency : Impact of efficiency of a PCR assay on the quantification results ,Role of amplification efficiency in validation of assays , Primer and probe design, and synthesis

UNIT VII : TEST OF COMPETENCE & PROFICIENCY

Our test will be based on the theory, practical and interpersonal skills for industry or research. Your certificate will be read with evaluation and detailed report.



PROGRAM 2 : TRAINING WORKSHOP ON RE-COMBINANT DNA TECHNOLOGY

The overall goal of the Training Workshop on rDT program is to provide the trainee with the skills they will need to provide appropriate cloning & expression technique for Cutting a piece of DNA from one organism and inserting it into a vector where it can be replicated by a host organism.

COURSE CONTENT

Unit I Nucleic Acid Extraction, Quantitation of Nucleic Acid Integrity

Extraction of both DNA & RNA , Qualitative analysis by electrophoresis, gel Docking and image analysis. Quantitative / DIN or RIN Analysis by Spectrophotometer/ Nano Drop/ Bio-Analyser.

Unit II - Bioinformatics

Primer Designing, Vectors , Selection of Restriction Sites, Virtual PCR, Bioinformatics tools & Techniques, Gel Analysis Software, Real Time PCR Primer Design, Q-PCR Data Handling, Sequence Data Analysis .

Unit III - Construction of Plasmids as Vector

Unit IV - r-DNA Technology :

Isolation of pUC18 plasmid from TOP10-pUC18 E coli cells, Restriction digestion of pUC 18 and λ DNA , Purifying pUC18/Hind III/ EcoR I digest by gel elution , Ligating the linearised plasmid - pUC18 and the insert –λDNA, Preparation of competent cells , Transformation of TOP10 cells with the pUC18-λDNA ligated product. Colony PCR : To amplify the inserted λDNA digest in pUC18 vector

Unit V - Genetic Engineered Clone Confirmatory Assay

Confirmation By PCR and its Optimisation





PROGRAM 3 : TRAINING WORKSHOP ON DNA METHYLATION STUDIES

The Genetic Methylation Studies is the study of the effects of chemical and physical agents on genetic material . Genetic toxicology helps discern the possibility of heritable mutations, developmental defects, cancer initiation, ageing and other long-term adverse genetic effects .

COURSE CONTENT

Unit I Nucleic Acid Extraction, Quantitation of Nucleic Acid Integrity

Extraction of both DNA & RNA , Qualitative analysis by electrophoresis, gel Docking and image analysis. Quantitative / DIN or RIN Analysis by Spectrophotometer/ Nano Drop/ Bio-Analyser.

Unit II - Bioinformatics

Primer Designing, Vectors , Selection of Restriction Sites, Virtual PCR, Bioinformatics tools & Techniques, Gel Analysis Software, Real Time PCR Primer Design, Q-PCR Data Handling, Sequence Data Analysis .

Unit III - Role of PCR & Real Time PCR in DNA Methylation

PCR & Optimisation for the Expression of Lethal Gene :- Optimisation of Conventional , Nested and PCR PCR Multiplexing Methods , Run of Conventional PCR , Data Analysis .

Real Time PCR & Applications: Introduction to Real-time PCR & Applications , Real-time PCR reaction setup , Construction of a standard curve: Biostatistics principles; Linear regression , Standard melt curve analysis ,High resolution melt curve analysis . Analysis of SYBR Green real-time PCR results

Unit IV : Methylation Studies by PCR

Methylation in DNA : Bisulfites modification of DNA , Bisulfites modification in nanogram quantities of DNA ,DNA Methylation PCR array

Methylation Specific PCR Technology :- PCR & Applications, Optimisation of PCR, Troubleshooting in PCR applications , Single Nucleotide Prime Extension

Unit V - Methylation Analysis by Q-PCR Assay

UNIT VI : TEST OF COMPETENCE & PROFICIENCY




PROGRAM 4 : TRAINING WORKSHOP ON PROTEIN PURIFICATION STRATEGIES

As a career, genetic toxicology provides the excitement of science and research while also contributing to the well-being of current and future generations. • As a toxicologist you may participate in basic research using the most advanced techniques in molecular biology, analytical chemistry, and biomedical sciences; You may work with chemical, pharmaceutical industries.

Course Content

Unit-1 Introduction to Protein Extraction :- Acid Base Equilibrium, pH, Buffer System, Charge, pI and pKa Value, Quantitative determination of biomolecule, mini scale bacterial protein extraction, protein extraction from plant source or other biological source

Unit -2 Introduction to Protein Purification :- Sample solvents, Column selection, Partition coefficient, Mobile phase selection, Gradients, Effect of flow rate, Temperature effects and Sample preparation.

Unit - 3 Challenges in Protein Purification

Unit - 4 Protein Purification by Affinity Chromatography:- Affinity Chromatography ( IMAC, GST Tagged Purification, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G )

Unit –5 Ion Exchange Protein Purification :- Ion Exchange Chromatography ( Mono-Q / Q Sepharose Fast Flow / Resource Q / Q Sepharose XL / DEAE Sepharose / SP Sepharose / CM Sepharose )

Unit -6 Reverse Phase Protein Purification : - Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins

Unit -7 Product Development : Valuable Protein Extraction From Bio-waste like ; Protein Extraction for Hair





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PROGRAM 5 : TRAINING WORKSHOP ON AMINO ACID ANALYSIS

Amino acid analysis is a fundamental biochemical technique used for the determination of the amino acid composition or content of proteins, peptides and other pharmaceutical or biological preparations.

COURSE CONTENT

Unit I : Sample Preparation For Amino Acid Analysis :- A preliminary step, required for the proper separation of amino acids and peptides, consists in finding a suitable, partitioning scheme of the extract between various solvents, in order to remove the unwanted compounds, such as: polysaccharides, lipids, phenols and others.

Unit II : Hydrolysis and Derivitisation of Amino Acid :- Liquid Phase Hydrolysis, Vapour Phase Hydrolysis, Pre Column Derivtisation, Post Column Derivitisation

Unit III : Separation of Amino Acids :- Separation of derevitised amino acids, Separation of non deriveitised amino acids, Separation of Amino Acid by Chromatography

Unit IV : Determination of Amino Acid :- Determination of amino acid after hydrolysis, Determination of amino acid before hydrolysis, Protein & Peptide Quantitation by spectrometry, Total Amino Acid Analysis, Free Amino Acid Analysis

Unit V : Reverse Phase Amino Acid Chromatography : - Reverse-Phase HPLC Separation of Amino Acid

Unit VI : Amino Acid Analysis of Food or Biological Sample





PROGRAM 6 : TRAINING WORKSHOP ON PROTEIN CHARACTERISATION

Protein Characterisation Workshop will provide hands-on laboratory exercises and instruction on protein characterisation strategies for biotechnology products, especially proteins. Participants will integrate theory with practice in areas such as protein extraction techniques, sample preparation, SDS-PAGE, Iso Electric Focusing, 2-D Electrophoresis and Western Blot Analysis of proteins.

COURSE CONTENT

Unit I : Protein Extraction :- Acid Base Equilibrium, pH, Buffer System, Charge, pI and pKa Value, Quantitative determination of biomolecule, mini scale bacterial protein extraction, protein extraction from plant source or other biological source

Unit II : Protein Estimation :- Protein Estimation by Lowry Method / BCA Method / Bradford Method

Unit III : Protein Characterisation by SDS-PAGE:- Basics of SDS-PAGE, Acetic-Acid Urea Polyacrylamide Electrophoresis of basic proteins

Unit IV : Western Blot Analysis : Protein Analysis by Western Blot

Unit V : Iso Electric Focusing of Proteins :- Iso Electric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels

Unit VI : 2-D Protein Electrophoresis : - Preparation of Protein sample for 2-D Electrophoresis, Protein solubility in 2-D Electrophoresis, Disruption of Di-sulphide bridges, Two Dimensional Polyacrylamide Gel Electrophoresis of Proteins using pH Gradient in First Dimension.

Unit VI : Data Analysis : Documentation of Gels / Membrane with Gel Documentation System, Gel Analysis Software and data analysis, Zymography of proteins.





PROGRAM 7 : TRAINING WORKSHOP ON FOOD ADULTERATION ANALYSIS

The purpose of the training programme is to upgrade skills and generate trained human resource in the food industry. The Training will give an opportunity to have hands-on experience in Food Adulteration Analysis. Food Analysis Workshop will provide hands-on laboratory exercises and instruction on separation and analysis strategies for adulterants in food samples .

COURSE CONTENT

Unit I : Quality Control Procedure For Food Adulterants :- Food Safety & Standards Act, 2006, Function, Duties & Responsibilities of Food Safety Regulators,Food Safety Management System

Unit II : Food Adulterants Analytical Methods & Sample Preparation :- Food Adulterants & Limits, Analytical Methods for the detection of food adulterants, Sample Preparation for food analysis

Unit III : Analysis of Food Adulterants by HPLC :- Basics of HPLC - Sample Preparation , Gradient Making ,Parts of HPLC , Troubleshooting and Maintenance , Operating Procedure of HPLC , Run the sample in HPLC . Data Analysis

Unit IV : Analysis of Food Adulterants by GC :- Basics of GC - Sample Preparation , Parts of GC , Troubleshooting and Maintenance , Operating Procedure of GC , Run the sample in GC , Data Analysis

Unit V : Sample Analysis : Participants will analyse the following samples

» Analysis of BHT in Food Samples » Analysis of Benzoic Acid in Food Sample » Analysis of caffeine in Beverages » Analysis of Mycotoxin in Food Product





PROGRAM 8 : TRAINING WORKSHOP ON QUALITY ANALYSIS OF BEVERAGES

The purpose of the training programme is to upgrade skills and generate trained human resource in the food industry. The Training will give an opportunity to have hands-on experience in Alcoholic Products Analysis. Alcoholic Product Analysis Workshop will provide hands-on laboratory exercises and instruction on separation and analysis strategies for quality standards of alcoholic products . Participants will integrate theory with practice in areas such as adulterants analysis techniques, chromatography theory and systems, sample preparation, type of columns, column selection, buffer selection, Gas and High Pressure Liquid Chromatography.

COURSE CONTENT

Unit I : Quality Control Procedure For Alcoholic Products :- Food Safety & Standards Act, 2006, Function, Duties & Responsibilities of Safety Regulators,Food Safety Management System

Unit II : Chemical Analysis Preparation :- Acidity Testing, IBU Testing, Specific Gravity, Alcoholic Content Testing, Antioxidant Testing, Post Fermentation Analysis, Colour, Turbidity Testing, pH Testing, Dissolved Oxygen etc.

Unit III : Analysis of Adulterants by HPLC :- Basics of HPLC - Sample Preparation , Gradient Making , Parts of HPLC , Troubleshooting and Maintenance , Operating Procedure of HPLC , Run the sample in HPLC . Data Analysis

Unit IV : Analysis of Adulterants by GC :- Basics of GC - Sample Preparation , Parts of GC , Troubleshooting and Maintenance , Operating Procedure of GC , Run the sample in GC , Data Analysis

Unit V : Chemical Analysis Procedures :- Procedures For the analysis of Alcohol by volume , Reducing Sugar, Aldehydes, Esters and Organic Acid ( Citric Acid, Acetic Acid, Malic Acid, Tartaric Acid ) Anthocynin, Total Phenolics, Sorbic Acid, Total Sulfidethanol, methanol, polyphenols, acetaldehyde, glutathione, proteins, several colloids etc

Unit VI : Sample Analysis : Participants will analyse the following samples

» Analysis of Total Alcohol in Whisky & Beer » Analysis of Sorbic Acid in alcoholic product » Analysis of Protein in Alcoholic Product » Analysis of Total Phenolics

UNIT VIII : TEST OF COMPETENCE & PROFICIENCY




PROGRAM 9 : TRAINING WORKSHOP ON ROLE OF DNA IN FORENSICS

he purpose of the training programme is to upgrade skills and generate trained human resource in the related sector. The Training will give an opportunity to have hands-on experience in Forensic DNA Analysis.

Forensic DNA Analysis Training will provide hands-on laboratory exercises and instruction on forensic DNA Analysis strategies for forensic samples, especially DNA. Participants will integrate theory with practice in areas such as DNA Extraction, DNA Analysis, PCR Reactions, Q-PCR Reactions and dHPLC Method for forensic analysis.

COURSE CONTENT

Unit I : Sample Collection, Law & Procedures, Record Keeping of Forensic Samples :- Acid Base Equilibrium, pH, Buffer System, Charge, pI and pKa Value, Quantitative determination of biomolecule, mini scale bacterial protein extraction, protein extraction from plant source or other biological source

Unit II : DNA Extraction, Quantitation & Optimisation : Extraction of Nucleic Acid , Gel Electrophoresis , Quantitative and Qualitative Analysis of Samples , Troubleshooting of Nucleic Acid Extraction

Unit III : Primer Designing & Selection Topics include: Primer designing for cloning & Expression , Primer Selection for Molecular Marker Analysis

Unit IV : STR Analysis of Forensic Samples :- Basics of DNA Profiling, Advantage of STR Marker, Position of STR Marker in Human Chromosome, STR Analysis, Variation of STR among individuals.

Unit V : Forensic Data Analysis : Gel Analysis Software, Similarity- Dissimilarity Matrix, Dendogram Generation and data analysis

Issues & Challenges in Forensic DNA Analysis:





PROGRAM 10 : ROLE OF TOXICOLOGY IN MEDICO LEGAL FORENSICS

The purpose of the training programme is to upgrade skills and generate trained human resource in Forensic Science. The Training will give an opportunity to have hands-on experience in Forensic Toxicology Analysis.

Forensic Toxicology Workshop will provide hands-on laboratory exercises and instruction on forensic toxicology sample analysis strategies for detection of toxic compound in forensic sample. Participants will integrate theory with practice in areas such as sample preparation technique, detection of lethal dose in biological samples, chromatography strategies, method development and validation.

COURSE CONTENT

Unit I : Sample Collection, Law & Procedures, Record Keeping of Forensic Samples :- Acid Base Equilibrium, pH, Buffer System, Charge, pI and pKa Value, Quantitative determination of biomolecule, mini scale bacterial protein extraction, protein extraction from plant source or other biological source

Unit II : Basics of Forensic Toxicology :- Introduction of Pharmacology, Drug, Poison & Metabolite, Pharmacokinetics of Drug ( Liberation, Absorption, Distribution, Metabolism & Elimination ), Drug Actions in human body, Forensic Toxicology Analytical Tools & Techniques

Unit III : Basics of Chromatography :- Analysis of samples by HPLC & Gas Chromatography : Introduction to HPLC Pump , Introduction to Auto Samplers and Injectors , Introduction to HPLC Detectors , Introduction to GC, GC Detectors, GC Columns, HPLC Columns , Tubing , Injectors , Introduction to Chromatography Software .

Unit IV : Chromatography Separation of Toxins - Column Chromatography , TLC , HPLC , Gas Chromatography , Solid Phase Extraction etc.

Data Analysis & Validation :

» Applied statistical concepts » Data analysis » Toxicology reports & case evidence » Quality Assurance » Method Optimisation





PROGRAM 11 : TRAINING WORKSHOP ON BIO SEPARATION BY HPLC

The purpose of the training programme is to upgrade skills and generate trained human resource in the related sector. The Training will give an opportunity to have hands-on experience in HPLC Chromatography and Method Development. High Performance Liquid chromatography enables reliable, high‐precision trace analysis with high repeatability of non volatile assay . HPLC is one of the most widely used techniques for analysing hydrocarbon mixtures. HPLC is widely used in the Pharmaceuticals, Bio-Pharmaceuticals, Food, Agriculture, Environmental, Forensic, Biotech, & Chemical Industries.

SCOPE OF THE WORKSHOP TRAINING :

As a career, genetic toxicology provides the excitement of science and research while also contributing to the well-being of current and future generations. • As a toxicologist you may participate in basic research using

COURSE CONTENT

Unit I : Introduction to HPLC :- Introduction to chromatography and chromatographic process - four modes of chromatography - reversed-phase, normal phase, ion exchange and size exclusion, Instrument operation each part of an HPLC instrument in detail – including the solvent delivery system, sample injection, connecting tubing and fittings, commonly used detectors

Unit II : Method Development :- Sample solvents, Column selection, Partition coefficient, Mobile phase selection, Gradients, Effect of flow rate, Temperature effects and Sample preparation.

Unit III : System Suitability:- Resolution, Efficiency, Asymmetry, Capacity factor, Selectivity, Signal to noise ratio, Precision and accuracy, System suitability limits and manual calculation of parameters, setting up software to perform system suitability calculations

Unit IV : Care of the HPLC System and Columns :- Storing of the HPLC system, Column storage, Changing between mobile phases, Developing a clean-up and shut down method.

Unit V : Troubleshooting : - Order of troubleshooting, Examples of common problems and causes, Practical examples.

Unit VI : Routine Maintenance Topics include: Theory & practical maintenance of Pumps, Filters, Injector, and Detector.

Unit VII : Hardware / System Validation : - Hardware validation for pumps, degasser, auto samplers, column ovens and detectors, and overall system check.

Unit VI: II Method Validation :- Accuracy, Precision, Intermediate Precision, Specificity, Detection limit, Quantification Limit, Linearity, Robustness, Acceptance criteria, Samples with more than one active.





PROGRAM 12 : TRAINING WORKSHOP ON BIO SEPARATION BY G.C

The purpose of the training programme is to upgrade skills and generate trained human resource in the related sector. The Training will give an opportunity to have hands-on experience in Gas Chromatography and Method Development.

Gas chromatography enables reliable, high‐precision trace analysis with high repeatability. Gas chromatography is one of the most widely used techniques for analysing hydrocarbon mixtures. GC is widely used in the Food, Agriculture, Environmental, Forensic, Biotech, Fragrance, & Chemical Industries.

COURSE CONTENT

Unit I : Introduction to Gas Chromatography:- The theory of GC, The GC System, Column types and packings, Mobile phases, the chromatogram, Familiarisation with the instrument and basic Operation

Unit II : Method Development :- Approaches to method validation, Frequency and Troubleshooting, Sample Preparation, Column selection, Partition coefficient,, Temperature effects and Sample preparation

Unit III : System Suitability :- Resolution, Efficiency, Asymmetry, Capacity factor, Selectivity, Signal to noise ratio, Precision and accuracy, System suitability limits and manual calculation of parameters, setting up software to perform system suitability calculations.

Unit IV : Care of GC System & Column :- Storing of the GC system, Column storage, Changing between mobile phases, Developing a clean-up and shut down method.

Unit V : Troubleshooting:- Order of troubleshooting, Examples of common problems and causes, Practical examples.

Unit VI : Routine Maintenance :- Routine Maintenance Topics include: Theory and practicals, Flow Check, Column Change, Filters, Injector, and Detector.

Unit VII : Hardware / System Validation:- Hardware validation for injectors , Oven Temperature , auto samplers, Gas Ports and detectors, and overall system check.

Unit VIII : Method Validation:- Instrument Detection Limits and Method Quantitation Limits, Accuracy, Precision, Intermediate Precision, Specificity, Detection limit, Quantisation, Limit, Linearity, Robustness, Acceptance criteria, Samples with more than one active.





DURATION OF TRAINING PROGRAM: 40 Hrs or 1 to 2 Weeks

TRAINING FEE FOR INDIAN NATIONAL : INR 6,000 / ( For Single Workshop )

WHO MAY APPLY : BSc / B.Tech / MSc / MTech in any Chemistry / Forensic Science / Pharmacy Life Sciences / Biotechnology studying or completed students

LAB TIMINGS : Session I : 9 A.M to 1 P.M Session II : 1 P.M to 5 P.M

HOW TO APPLY –

Details of Documents For Registration :

1. Scan copy of Any identity proof along with University / College Identity Card / Aadhar Card etc. 2. Filled Registration form with photograph ( Given in Last Page of Brochure ) 3. Registration fee will be Rs 1000 / - paid through on line 4. After payment send all documents with payment receipt at : [email protected] or Whatsapp - 9891179928 5. We will send confirmation within specified time through e.mail or remind us if not received at our email.

ON LINE PAYMENT METHOD :

Payment By Internet Banking Scan PAY TM Code

Beneficiary Name - Allele Life Sciences Private Limited Account Number - 61071508494 IFSC Code - SBIN0031811 Bank Name - State Bank of India Bank Address - SBI, 14/15, Sector-18, Noida, UP - 201301

Or Pay Through UPI Address - allelelifesciences@upi




Registration Form

Name of Training Program : Expected Date of Joining :Candidate Details :

Name: ………………………………………………………………………………………………. Father’s Name: …………………………………………………………………………………… Address : ………………………………………………………………………………………….. Contact No : ………………………………………………………………………………………. Email: ………………………………………………………………………………………………. Institution : ………………………………………………………………………………………… Qualification : ………………………………………………………………………………………

Terms & Conditions :

1. The admission to training / internship programs will be confirmed after the payment of registration fee along with documents.

2. The registration fee deposited is completely non refundable. 3. The industrial training fee includes the cost of chemical , reagents and study material costs. 4. I will deposit the service charges as decided by the company at the time of joining date of training program. 5. Students have to bear their own boarding/lodging /conveyance charges. We facilitate students in finding proper paying guest arrangements. 6. The trainees will have to bring their own lab coat and wear them all the time in the campus. 7. Trainees are selected on first come first serve basis 8. Trainees will maintain adequate discipline inside the lab premises. 9. Company will not be responsible for any medical, legal issues during the internship tenure.

DECLARATION

I________________________________from____________________________________ hereby declare that all statement/information given in the application form are true to the best of my knowledge and belief . I will strictly abide by the norms/lab etiquette during the training

Signature

Place: ______________ Date: ________________________

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LAB FACILITY :

Our State of art facility is located in Industrial Area of Noida (NCR ) . The lab / research facility is Total : 6000 Sq Feet

Affymatrix & Agilent Microarray Platform Gene Expression Studies, Biomarker, Sequencing

Real Time PCR ( ABI ) Gene Expression, Sequence Detection

PCR ( ABI, Biorad , Eurofins ) - 5 in numbers Amplification of nucleic acids

Bioanalyser & Spectrophotometer ( Thermo ) Quantification of Nucleic Acids

Gel Documentation System Visualisation of Nucleic Acids, PCR Products etc.

Electrophoresis & Power Supply ( Biorad ) - 7 Sets Separation of Nucleic Acids & Other Arrays

DNA Concentrator ( Thermo Speedvac ) Nucleic Acid Extraction

Centrifuge, High Speed Centrifuge - 8 Nos Sample Preparation

PCR Station and other accessories

Biorad Profinia Affinity Chromatography Affinity Chromatography - IMAC, GST, Antibody

Biorad Biologic Low Pressure Chromatography Size Exclusion, Ion Exchange, Affinity etc.

Preparative HPLC ( Thermo ) , Agilent 1100 Bulk Protein Purification & Analysis

GE Amersham 2-D Electrophoresis System Protein Characterisation

Immunoblot, SDS-PAGE , Biorad HV Powerpac Visualisation of Nucleic Acids, PCR Products etc.

Mass Spectrometry , ELISA, Immunoassay Protein Identification

Cryo Preservation Facility & Common Facility Sample Storage & Preparation

Agilent HPLC System - PDA, FLD & ECD Detector Separation and analysis of molecules

Agilent GC with FID & FPD Detectors Separation and analysis of molecules

Thermo Prep HPLC with Dual Pump & UV-Vis Bulk Purification & Analysis

Shimadzu GC with FID & NPD Detector Separation and analysis of molecules

Triple Quad GC-MS System ( Agilent ) Analysis of Semi Volatile & Volatile Compound

LC-MS-MS ( API Sciex ) Analysis of Non Volatile Compound

Varian Carry Spectrophotometer Analytical Tool for various purpose

Thermo Helios Spectrophotometer Analytical Tool for various purpose

Vacuum Rotary Evaporator ( Buchi ) Sample Preparation



OTHER ANALYTICAL FACILITY :

Refractometer , Flame Photometer ( Toshniwal), Karl Fisher Titrator (Sistronics), Potentiometer, Polarimeter , Tintometer ,Viscometer , Kjeldahl Distillation Unit , Kjeldahl Digestion Unit , Ion Selective for Fluoride Analysis ( Thermo Orion ) , Nephalometer , Soxhlet Extraction , Rotatory Vaccum Evaporator with chiller , etc.

Microbiology & Cell Culture Facility :

Vertical Laminar Air Flow ( 4x2x2) , Horizontal Laminar Air Flow ( 2x2x2) B.O.D. Incubator ( Julabo ) , CO2 Incubator ( Jauan ) , Orbital Incubator Shaker, UV Chamber , Incubator, Colony Counter , Colorimeter , Muffle Furnace , Hot Air Oven , Desiccators, Binocular Microscopes and , Lypholizer Biochemistry / Organic Synthesis Chemistry Lab :

Spectrophotometer ( Thermo Heleus Alpha ) , Analytical Balance ( Sartorius ), Ph Meter ( Thermo Orion ) , Ion Selective (Thermo Orion) , Conductivity Meter ( Thermo Orion ), Dissolved Oxygen Meter ( Thermo Orion ) , Turbidity Meter, Autoclaves, Hot Air Oven , Hot Plate , Magnetic Stirrers , Pipette Washer , Shaking Machine , Water Bath , Colorimeter , Flame Photometer , etc.

Lab Water Purification : Millipore Milli Q System

Clinical Biology Lab : Haematology Analyser , Automatic Immunoassay, Haematology HPLC Biorad Variant



ALLELE LIFE- SCIENCES PRIVATE LIMITED C-59, Sector-10, Industrial Area,NOIDA, Gautam Budh Nagar,Uttar Pradesh India - 201301 E.mail - [email protected] , [email protected] - +91 - 9891179928 | 8377082003 | 9319466389WhatsApp - +91 - 9891179928 | 8377082003

Corporate Identity Number : UD85195DL2006PTC150518 ,

Registered Under Indian Companies Act , 2003, Ministry of Corporate Affairs, Govt. Of India.

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