Postdoctoral  Trainee  Exchange  Program  between  UTSW  and  the  First  Affiliated  Hospital  of  Sun  Yat-­‐sen  University  

 Trainee:  Xiuyu  Leng  

Mentor:  Jay  W.  Schneider    

UTSW,  Dallas,  Feb  2013

Isoxazole (ISX) !Cardio-regenerative

small-molecules!

Lab  Research  Focus:  

UTSW  chemical    library  

Project  1.    ISX  and  fibrosis  

Results:    Isx  a;enuates    pro-­‐fibro@c  changes  induced  by  Ang  II  in  vivo.    

.    ACTA2  COL  DAPI

QuesUon:    Can  ISX  reduce  Ang  II  induced  fibrosis?  

Ang  II

Project  2.    Studies  of  heart  repair  by  magne@c  resonance  imaging  (MRI)

Pre-­‐  treat  with  ISX  or  VEH  for  14  days

 D0                                D3                                                    D7                                                                                          D14  

D1,  Injury  (MI)

   Cardiac  MRI  

Treat  with  ISX  or  VEH  for  totally  28  days

Before  MI AVer  MI

Study  design:  

Project  3.    Studies  of  GPR68

GPR68  ACTN2  DAPI  

FLAG    TOPONINI  DAPI  

TransducUon  of  GPR68    adenovirus  in  neonatal  rat  cardiomyocytes      

 GPR68  and  ACTN  staining  in  mouse  heart

Project  4.    ISX  and  GPR68  in  neurogenesis  

Coupling Hippocampal neurogenesis to brain PH through proneurogenic small molecules that regulate proton sensing G protein-coupled receptors.

ACS Chemical Neuroscience. 2012.

TransducUon  of  GPR  68  adenovirus  in  HCN  cells    

FLAG  TUJ1  DAPI

•  Acquired  exper@se  in  lab  techniques:  –   Cell  culture,  RT-­‐PCR,  immunocytochemistry,  protein  blo^ng  and  perfusion  

fixa@on  of  hearts,  etc.    

•  Addi@onal  exci@ng  technologies:    –  Microarray,  proteomics,  NMR(Nuclear  magne@c  resonance  spectroscopy),  etc  

•  Observa@on  of  pharmacological  regula@on  of  heart  repair  following  injury  

•  Co-­‐authored  one  paper  •  Next  paper  in  prepara@on  

–   (A  cardiogenic  small-­‐molecule  that  regulates  myocardial  metabolism,  heart  func@on  and  survival  by  Schneider  et  al)  

•  Contribu@on  of  GPR68  adenovirus  data  to  future  papers.      

Summary  of  two  years’  training  in  UTSW