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TORELANCE LEVEL OF DIFFERENT CABBAGE VARIETIES TO BLACK ROT
BY: MUNENE DAVID M.
A22/0081/2009
SUPERVISOR: PROF. DANIEL MUKUNYA
Cabbage is the most valued and the most used vegetable in the world
Of all the crucifers, cabbages are the most affected by black rot caused by a bacteria Xanthomonas campestris pv campestris
Different seed companies have breed for resistant varieties (F1) but farmers are still suffering losses related to black rot
This means there is a need to evaluate the most resistant variety that can minimize losses encountered by the farmers around Kabete
Despite breeders effort to breed for resistant varieties of cabbage, black rot disease has remained to be a threat in most parts of the world, especially in small scale
Production(21MT/ Ha) has remained much lower than the yield potential (26-75 MT/Ha depending on variety) despite high demand, good market prices and health benefits associated with cabbages
Infected cabbages are also prone to other pathogens leading to high storage losses
This is as result of break down of resistant gene following the continued mutation of the bacteria over time
General objective; Evaluation of the most tolerant cabbage varieties against black
rot commonly grown in Kenya
Specific objective; Determine the incidence of black rot in different cabbage
varieties
Four cabbage varieties- Riana, Fanaka F1, Pructor F1, Queen F1
1 kg DAP 2kg CAN pesticides
70% alcohol(100ml) 3% Sodium hypochlorite
(50ml) Sterile distilled water Empty Petri dishes 12 plates of nutrient agar Wire loop Spirit lamp 12 universal bottles Clean distilled water Teat pipette/ dropper
Planting and establishment of cabbages in the field Collection of young black rot infected cabbage material
from the field station for bacterial isolation in the laboratory Isolation of bacteria using aseptic method Sub-culturing of bacteria to produce clean pure colonies Serial dilution to achieve different numbers of colony
forming units(c.f.u) Bacterial inoculation to cabbage plants Data collection
Bacteriology exercise book, fourth year, isolation of bacteria http://go.warwick.ac.uk/wrap Edward J. Sikora, Extension Plant Pathologist, Professor, Entomology and
Plant Pathology, Auburn University International Rules for Seed Testing Annexe to Chapter 7: Seed Health
Testing Methods HCDA ( Horticulture Crop Development Authority), 2007 Report http://dx.doi.org/10.1094/PDIS-94-3-0298 Massomo SMS, Mabagala RB, Swai IS, Hockenhull J, Mortensen CN .
“Evaluation of varietal resistance in cabbage against the black rot pathogen, Xanthomonas campestris pv. campestris in Tanzania.” Crop Protection 23,4(2004): 315-325.
Chupp C. “Black rot of cabbage.” Manual of Vegetable Plant Diseases. New Delhi, India : Discovery Publishing House, 2006. p. 132-133
Williams PH. "Black rot: a continuing threat to world crucifers." Plant Disease 64.8 (1980): 736-742.
"Black rot of cabbage and other crucifers." Integrated Pest Management. University of Illinois Extension. Dec 1999.
Activity Dec Jan Feb March April May
Raising
seedlings in the
nursery
watering
Transplanting
gapping
Top dressing
Data collection
proposal
inoculation
Data analysis
Final
presentation