FHSC1214 Fundamental of Cell Biology
TitleInvestigation of the Enzymatic Effect of Materials on
Hydrogen Peroxide
Solution___________________________________________________________________________
Objective To investigate the enzymatic effect of various
materials in the hydrogen peroxide solution.
ApparatusBeaker, test tubes, water bath of 95C and Bunsen
burner.
MaterialsFresh liver, potato cubes, manganese dioxide, hydrogen
peroxide, wood splints and parafilms.
Procedure1. Six fresh empty test tubes were labelled 1, 2, 3, 4,
5, 6 and were stood in a rack.2. A razor blade was used, the
provided liver was cut into several pieces of roughly 0.8 cm x 0.8
cm x 0.5 cm.3. One piece of liver was placed into tube 1.4. The
second piece of liver was placed into the bottom of tube 2. A
wooden splint was used to gently spread the liver, without mashing
it, over as wide an area as possible of the bottom of the test
tube. Tube 2 was placed in the water bath of 95C for about five
minutes.5. A weighing balance was used, two 0.5g portions of
manganese dioxide powder were measured out and each portion was
pour into tube 5 and 6.6. Tube 6 was put in the water bath for five
minutes.7. Tube 2 and 6 were let cool after five minutes.8. The
third piece of liver was put on a white tile. With a mortar or
pestle, it was gently mashed into pulp. The pulp was scoop into
tube 3.9. The potato cubes was cut into roughly 0.8 cm x 0.8 cm x
0.5 cm. One of the cube was placed into tube 4.10. Another six
fresh empty test tubes were prepared and were stood in rack. 5 cm3
of hydrogen peroxide was put into each of the tube.11. Next,
hydrogen peroxide was quickly added into test tube 1, 2, 3, 4, 5
and 6. 12. The parafilm provided was quickly stretched and seal the
mouth of the test tubes by stretching the film over it. Step 11 and
12 should be done quickly.13. The test tubes were left for 20
minutes.14. After 20 minutes, a glowing splint was inserted into
each tube one at a time by penetrating the parafilm with it.15. All
the observations were recorded in Table 1. The observations on each
test tube were immediately recorded after the reaction has
started.
Data and Observation
Test tubeContents with 5 cm3 hydrogen peroxideObservations
before and after using wood splint
1Fresh liver
2Boiled liver (cooled)
3Pulped liver
4Potato cubes
5Manganese dioxide (untreated)
6Boiled manganese dioxide (cooled after heating)
Discussion
2
