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Globus, UVA, June 2005, JNL
THz Characterization of Lysozyme at Different Conformations
This work was supported by the U.S. DOD under Contract DAAD19-00-1-0402,and in part by U.S.Army NGIC Contract # DASC01-01-C0009
aDepartment of Electrical and Computer Engineering, University of Virginia,351 McCormick Road, PO Box 400743, Charlottesville, VA 22904, USAbElectronics Division, US Army Research Office, U.S. Army Research Laboratory,PO Box 12211, Research Triangle Park, NC 27709-2211, USA
cDepartment of Chemical Engineering, University of Virginia, PO Box 400741,Charlottesville, VA 22904, USA
Tatiana Globus*a, Tatyana Khromova a, Rebecca Lobo a, Dwight Woolard b, Nathan Swami a, and Erik Fernandez c
Globus, UVA, June 2005, JNL
MOTIVATION and GOALS
There is a general need for fast and less expensive techniques that can provide useful structural information on bio-materials.The capability of recently emerged THz spectroscopy to detect the hydrogen bond low-frequency vibrations opens potentially many applications for this technique includingmonitoring of folding-unfolding processes of proteins. Our goal is to demonstrate the capability of THz spectroscopy to discriminate between different protein conformations and to evaluate it possible application for monitoring folding-unfolding process of lysozyme, a easily available protein and whose structure has been well studied by other methods.
Globus, UVA, June 2005, JNL
Lysozime is a globular α+β protein with approximately 45 % of α-helix type secondary structure and ~ 20% of β-sheet structure.
In addition it has ~ 25% various turn conformations that generallyexist in globular proteins and ~ 13% of unordered or “random coil”secondary structure [2].
Lysozyme also adopts specific tertiary structure known from X-ray diffraction and nuclear magnetic resonance (NMR).
After many years of study protein folding-unfolding processes stillnot completely clear.
LYSOZYME
Globus, UVA, June 2005, JNL
Available Techniques for Protein Structure Characterization
While the three-dimensional structure of proteins is determinedby X-ray crystallography, it requires a well ordered crystallynesamples.
The nuclear magnetic resonance (NMR) spectroscopy can be applied to proteins in solution. However the interpretation is very complex and is limited to small proteins.
Alternative methods, circular dicroism (CD) and vibrational (infraredand Raman) spectroscopy are not able to generate structures at atomic resolution but provide structural information on proteins,especially on secondary structure.
High quality spectra can be obtained with relatively by recentlydeveloped Fourier Transform Infrared FTIR spectroscopy
Globus, UVA, June 2005, JNL
In FTIR spectroscopy, conformation sensitive the amide I band (1600-1700 cm-1) is used to determine the type of the secondary structure of proteins.Each type of secondary structures gives rise, in principle, to a different C-O strechfrequency in the amide I region of the spectrum of the peptide linkages in the backbone structure. Three analytical procedures are used: Fourier self-deconvolution, second derivative, and band curve-fitting. Assignments for deconvoluted Amide I subbands is based on X-ray data to determine the distribution of secondary structures.The number of sub-bands determined by deconvolution is usually do not exceed 7with the accuracy of analysis reducing for larger numbers.
Fourier Transform Infrared Spectroscopy (FTIR)
Globus, UVA, June 2005, JNL
Frequency, cm-10 1000 2000 3000
Abs
orpt
ion
coef
ficie
nt, c
m-1
0
500
1000
1500
2000
I Visible & near IRII -far IRIII -THz
Frequency, THz3 10 30 60 90
III I I
DNA Absorption Spectrum
Absorption spectrum of DNA (our results).
Regions I & II (Studied wellby IR & Raman)- resonances dueto short-range, high energyinteractions
in THz Region III – morespecies specific spectral features of bio molecules are found
FTIR spectroscopy produces high quality spectra in the region II,and can separate overlapping subcomponents in the spectra
Globus, UVA, June 2005, JNL
In the THZ region III (0.1-10 THz or 2 - 300 cm-1), absorption spectra reflect low-frequency molecular internal motions or vibrations involving the weakest hydrogen bonds and/or non-bonded interactions between different functional groups within molecules or even between molecules.The resonant frequencies of such motions – phonon modes- are strongly dependent on molecular structure
INTERNAL MOLECULAR VIBRATIONSINTERNAL MOLECULAR VIBRATIONS
Bonds
Bond angles
>21 THz (700 cm-1 )
6-27 THz (200-900 cm-1 )
< 9 THz (<300 cm-1 )Torsion angles
FrequenciesVibrations of
Region III
Globus, UVA, June 2005, JNL
WEAK HYDROGEN BONDS
Weakest hydrogen bonds, shown by dots:-C-H ......O--C-H ......N-
-C ...... H-N--C ...... H-O--N-H......O=C-
- weak and have only ~ 5% of the strength of covalent bonds
- multiple hydrogen bonds stabilize the structure of bio-polymers
- hold the two strands of the DNA double helix together, or hold polypeptides together in different secondary structure conformations.
Globus, UVA, June 2005, JNL
Why THz?
Liquid water absorptionLess water absorption (at list 2 orders)
compare to IR and far-IR. Lessoverlap with water or other analytesabsorption bands. Liquid samples can be characterized.Absorption bands are more narrow in the THz range than in the IR and overlapping of neighboring bands is less.
The availability of multiple resonances for the sensitive measurement of bio-molecule structure.
Spectra are more species specific.
THz spectroscopy reveals structural information quite different from all other methods: it is the only technique that can directly detect weakest hydrogen bonds and other non-bonded interactions within biopolymers.
Globus, UVA, June 2005, JNL
“THE WORLD OF THE DEAD OR OF FUTURE PUNISHMENT“M .N. Afsar &K.J.Button, “Infrared and Millimeter Waves,” V.12, Acad. Press, 1984
“Terahertz gap”The spectral range between the upper end of the microwave and the
lower end of the extreme far IR
Low energy of sources.Low energy of sources.Low absorptionLow absorption of of biological materialbiological material requires samples with requires samples with large area and thickness which is difficult to make beclarge area and thickness which is difficult to make because ause samples are too fragile.samples are too fragile.PoorPoor reproducibilityreproducibility of experimental results due to of experimental results due to multiple multiple reflectionreflection in measurement systems, responsible for in measurement systems, responsible for artificialartificialfeaturesfeatures; difficulties in sample ; difficulties in sample preparationpreparation, , ininstabilitystability of material.of material.TheThe absenceabsence of goodof good commercially availablecommercially available laboratory laboratory instrumentsinstrumentsPotentially promisingPotentially promising laboratory techniqueslaboratory techniques as as time resolved time resolved spectroscopy and spectroscopy and photomixingphotomixing technology technology are only recently are only recently emergedemerged
Globus, UVA, June 2005, JNL
Is there something in the very far IR spectra? ((initial prediction of initial prediction of vibrationalvibrational modes in polymer DNA in the 1modes in polymer DNA in the 1--100 cm100 cm--1 frequency range [ 1 frequency range [ E.W.ProhofskyE.W.Prohofsky, K.C. Lu, , K.C. Lu, L.L.Van Zandt and B. F. Putnam, Phys L.L.Van Zandt and B. F. Putnam, Phys LettLett., 70 a, 492 1979; K.V. ., 70 a, 492 1979; K.V. DeviDevi Prasad and Prasad and E.W.ProhofskyE.W.Prohofsky, Biopolymers, 23,1795, 1984]. , Biopolymers, 23,1795, 1984].
What are the reasons why researchers for 20 years failed to achieve reproducible results? Experimental results are not reproducible and are
contradictive. It was not clear what to expect. Can we improve the results?
Can we use the observed features for DNA characterization, identification and discrimination between species?
The key to answer all these questions: we need to know of what we are looking for.
Questions to answer:
Globus, UVA, June 2005, JNL
Maria Bykhovskaia, B. Gelmont
Energy minimum Normal modes Oscillator strengths Spectra
QUESTIONS:
What do we expect to find in the submillimeter wave range?What is the predictive power of the method?How sensitive are far IR absorption spectra to DNA structure?
Normal mode analysis is applicable to molecules with less than 30 base-pairs
IR active modes are calculated directly from the base pair sequence and topology of a molecule.InitialInitial approximation was generated and optimized by the approximation was generated and optimized by the program packages program packages JUMNA and LIGAND JUMNA and LIGAND (group of Prof. (group of Prof. LaveryLavery,, Inst.BiologieInst.Biologie Phys.Chim.ParisPhys.Chim.Paris).).
THEORETICAL PREDICTION OF THz ABSORPTION SPECTRA
Globus, UVA, June 2005, JNL
ENERGY MINIMIZATION AND NORMAL MODE ANALYSIS(in internal coordinates of a molecule)
Maria Bykhovskaia, B. Gelmont
E total = E Van der Waals + EElectrostatic + E HBonds + E Torsion + E Bond angles
Molecular potential energy approximated as a function of dynamic variables(q): torsion and bond angles.
Two BB--helicalhelical conformation conformation DNA DNA fragments (TA)12(TA)12 with different base pair sequences:
andand thethe AA--helixhelix of double stranded of double stranded RNA Poly[C]RNA Poly[C]..Poly[G]Poly[G]
AAAAAAAAAAAA ATATATATATATTTTTTTTTTTTT TATATATATATA
Van der Waals and electrostatic interactions; the energy of hydrogen bonds deformations; torsion rotation potentials; stretching deformations of bond angles and of bond length
Conformational energyincluding long distance interactions :
Globus, UVA, June 2005, JNL
LOW FREQUENCY NORMAL MODES
0 50 100 150 200 2500
5
10
15
0 50 100 150 200 2500
5
10
15
20Poly (dAdT) Poly (dTdA)
Poly (dA) Poly (dT)
0 200 400 600 8000
5
10
15
Num
ber o
f mod
es
0 200 400 600 8000
5
10
15
20
Vibrational frequency (cm-1)
360 normal modes were found for each sequence with 360 normal modes were found for each sequence with the density higher than 1 the density higher than 1 mode per cmmode per cm--11.. There is almost There is almost no overlapno overlap of of weak bond modesweak bond modes with vibrations with vibrations of of covalent bondscovalent bonds which have frequencies above 750 cmwhich have frequencies above 750 cm--11. .
Maria Bykhovskaia, B. Gelmont
Globus, UVA, June 2005, JNL
Absorption Spectra vs. Base Pair Sequence
α(ω) ∼ γω2 Σ (pk)2 / ( (ωk2 -ω2 )2 + γ2 ω2 ), the oscillator decay γk=2 cm-1 ,
the dipole moment
0 100 200 300 400 500
Poly(dAdT)Poly(dTdA)Poly(dA)Poly(dT)
0 100 200 300 400 500
Observed (Powell et al., 1987)
Abs
orpt
ion
Frequency (cm-1)
TheThe spectrum of optical activitiesspectrum of optical activities is veryis very sensitive to the DNA to the DNA base pair sequencebase pair sequence
iii
i me /ap ∑= ,
Maria Bykhovskaia, B. Gelmont
Globus, UVA, June 2005, JNL
Direct comparison of experimental spectrum (red) with theoretical prediction (blue) for a short chain DNA fragment with known structure.
Reasonably Good correlationvalidates both, experimental and theoretical results.From the width of the vibrational modes:
oscillator decay γ =0.5 cm -1
relaxation time τ =7 10-11 s
Many of the initial successful measurements of the THz absorption properties of biological materials have been performed at the University of Virginia.
Evidence of multiple resonances in THz transmission spectra with a high degree confidence in recognition of bio- molecules has been demonstrated.
Experiment and Modeling
Poly [C]- Poly [G] d=6.5 μm
Frequency, cm-1
10 12 14 16 18 20 22 24
Abs
orpt
ion
coef
ficie
nt, c
m-1
0
10
20
30
40 measured calculated, γ=0.5 cm-1, E perp Zcalculated, E // Z
Globus, UVA, June 2005, JNL
Bruker IFS-66 spectrometer (Hg- lamp source, He cooled Si-bolometer @ 1.7 º K). Vacuum systems are not shown.
Attachment for reflection measurements.Resolution 0.2 cm-1.Range of interest throughout 10 cm-1 – 25 cm-1.
Experimental set up
T.Globus, UVA
Globus, UVA, June 2005, JNL
THz FourierTHz Fourier--Transform (FT) spectrTransform (FT) spectroscopyoscopy
Spectral resolution at least 0.25 cm-1 to measure features with 0.5 cm-1
band widthHigh sensitivity (signal to noise) and reproducibility to provide standard deviation better than 0.3% to measure small signals
What is important?Good Instrument performance:
7 samples of BG (5 mg each)
Frequence,cm-1
10 12 14 16 18 20 22 24
Sta
ndar
d de
viat
ion
0.001
0.002
0.003
0.004
0.005
0.006
7 different samples
High transparency of substrates
10 15 20 25
Frequency, cm
0.87
0.89
0.91
0.93
0.95
0.97
0.99
1.01
Tran
smis
sion
CT DNA, 5 mg100%-linePC-membrane, d=5 mkm
-1
Sensitivity Resolution
Globus, UVA, June 2005, JNL
Serious problem at THz - all kinds of multiple reflectionsor standing waves in most of measurement systems because of large wavelengths of radiation. These effects cause artificial false resonances.
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.97
0.98
0.99
1.00
1.01
# 206.83, Gor-Tex, d=3 mm
Check for false resonances using thick plates from material with low absorption is important. Ideallyspectrum is close to cos form.
Challenging ProblemChallenging Problem
Globus, UVA, June 2005, JNL
Salmon DNA
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.68
0.70
0.72
0.74
0.76
0.78
0.80
0.82
0.84#220.29, 10/31/02#220.39, 10/31/02#222.12, 11/07/02#225.23, 11/14/02#227.07, 11/21/02#230.10, 12/11/02
Improved technique for solid film sample preparation:good alignmentreduced amount of material from 15-20 mg to 1- 3 mg for one samplereproducibility better than 0.5%
Period of 45 days
Long term reproducibility
Globus, UVA, June 2005, JNL
Hen egg white lysozyme (HEWL) has been purchased from Sigma (97% pure, balance primarily sodium acetate; catalog no. L6876; lot 65H7025).
Salt precipitation
Native lysozyme, HEWL was resuspended in 0.1M phosphate buffer to a protein concentration of 50-150 mg/mL.
Unfolded lysozymelysozyme have been prepared by have been prepared by salt (KSCN) precipitation. . HEWL was HEWL was resuspendedresuspended in 0.1M phosphate buffer to a protein concentration of in 0.1M phosphate buffer to a protein concentration of 50 mg/50 mg/mLmL and then and then KSCN was addedKSCN was added (0.2M in solution, pH adjusted to 2.14 (0.2M in solution, pH adjusted to 2.14 using 1M using 1M HClHCl) ) to induce unfolding and precipitation to form slurry.
Refolded lysozyme by removing of KSCN from slurry of unfolded lysozyme by centrifuging, dissolving in 0.1M sodium acetate buffer solution (pH 3.8 with 1M NaOH), second centrifuging to remove precipitate, ultrafiltration and desalting using size exclusion chromatography.
Material for studyMaterial for study
Globus, UVA, June 2005, JNL
Two standard procedures were used to prepare samples in unfolded conformation:
The thermal denaturation at 950C for at least two hours.In water the secondary structure of lysozyme undergoes a cooperative thermal unfolding transition .
Denaturation by dissolving in 6M guanidine cloride (GuHCl),pH 4.6, results in substantially unfolding state in which littlepersists secondary or tertiary structure and eliminates refolding process in unfolded lysozyme.Simple techniques has been developed to fabricate large area, thin,stable samplesThin, air dried protein films were prepared on polycarbonate (PC) filter membrane. Heat denaturated lysozyme was drying at 95oC. Material in the form of gel have been prepared by placing 100-200 μl of solution between two polyethylene films (PE )and stored in thefreezer at -20 oC
Lysozyme Denaturation. Sample Prep.
Globus, UVA, June 2005, JNL
Image of the Salmon DNA sample in polarizing microscope (free standing).
Film thickness about 10 μm.
Gel concentration 1:10.
DNA, as a rod-like polymer, spontaneously forms ordered liquid crystalline phases in aqueous solution with the long molecular axis preferentially aligned in one direction.
In drying process, DNA solution undergoes a series of transitions and film samples are characterized by their microscopic textures with periodic variations in refractive index and fringe patterns observed in polarizing microscope.
The film texture depends on the concentration of molecules in solution and on drying conditions.
Material texture
Globus, UVA, June 2005, JNL
Absorption coefficient at two orientations
Poly [A]-Poly[U], d=16 μm
Frequency, cm-1
10 15 20 25
Abs
orpt
ion
coef
ficie
nt, c
m-1
0
50
100
150
E perp Z E//Z
Documented strong anisotropy of optical characteristics ofbiological molecules at THz.
E
z
x+y
x+y
Ez
a b
Sample position with electric field (a) perpendicular and (b) parallel to the long-axis of the molecule z.
Change with sample rotation.Change with sample rotation.
Globus, UVA, June 2005, JNL
Biological materials in an aqueous form (or gel) can be characterized as well as in solids.
Signature is strong. Relative change in the peak up to 10-30%.Spectra are not disturbed by water absorption at THz (except at 18.6 cm-1).High sensitivity of spectra to orientation.
Lysozyme
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.18
0.20
0.22
0.24
0.26
0.28
0.30
0.32
0.34
30 deg90 deg 120 deg
Importance: all living matter is in a liquid form
Possible applications:
THz spectra of Biopolymers in water (gel)
structural characterization of proteins and DNA at THz; monitoring biological processes.
Disturbance at 18.6 cm-1 is due to
absorption of water vapor in air.
Globus, UVA, June 2005, JNL
LU 1169, long term reproducibility
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.82
0.84
0.86
0.88
0.90
07.01.04 (1)07.01.04(2)07.08.0408.05.04
Lysozyme Native, liquid 200 mg/ml
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.94
0.95
0.96
0.97
0.98
0.99
1.00 LN 1154,LN 1155LN 1161LN 1163
4 samples of LN by diluted HCl
LYSOZYME Native and Thermally Unfolded
Spectra of thermally denaturated (LU) lysozyme
A big difference observed between transmission spectra of native lysozyme obtained by diluted HCl and thermally denaturated lysozyme with the same amount of dry material
Resonance structure in four folded samples (LN) in diluted HCl is very weakwith the intensity of peaks ~0.5% - 1%, and the probability of errors is high.
Unfolded lysozyme has higher absorption (less transmission) with higher intensity of resonances. Spectra of unfolded samples demonstrate a good long term reproducibility at the same orientation. However, after storing for a month, significant changes in the measured spectrum observed.
Globus, UVA, June 2005, JNL
LU 1157, liquid, thermally unfoldedeffect of orientation
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.60
0.62
0.64
0.66
0.68
0.70
0.72
0.74
0.76
0 deg90 deg
LYSOZYME Thermally Denaturated (Unfolded)
Spectra of unfolded lysozyme are sensitive to orientation.
Globus, UVA, June 2005, JNL
Lysozyme Native and thermally unfolded, liquid
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.85
0.90
0.95
1.00
LN 1155 LU 1169
Spectra of a lysozyme sample unfolded with GuHCl differ from thermally unfolded.
Lysozyme unfoldedd with GuHCl #1177, liquid,two independent measurements
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.87
0.88
0.89
0.90
0.91
0.92
0.93
LYSOZYME Native and Denaturated with GuHCl
Globus, UVA, June 2005, JNL
LYSOZYME Unfolded with GuHCl and thermo-Unfolded
LU1177 with GuHCl & LU1169,Thermo-unfolded
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.82
0.84
0.86
0.88
0.90
0.92
0.94
LU1177 with GuHClLU 1695,thermo
Spectra of thermally unfolded and GuHCl unfolded lysozymes.
2 spectra show very close correlation with almost all resonant frequencies presentin both cases, however there are some differences: clearly additional absorptionpeak at 19.6 cm-1 in thermo-denaturated sample, and difference between two atfrequencies 23-24 cm-1.
Globus, UVA, June 2005, JNL
Possible applications of proteins THz spectroscopyWide-range of biomedical applications based on close relationship between structure and spectra, including monitoring changes in molecular conformation in real time
real-time analysis of protein binding for transport
protein binding with antibodies,
specificity interactions between proteins and nucleic acids
binding stability studies of drug-protein and vitamin-proteinsystems
disease diagnostic
Globus, UVA, June 2005, JNL
ConclusionsTHz spectra are sensitive to conformation change in proteins.
Absorption of unfolded lysozyme is higher in comparizon withthat of a native lysozyme.
Spectra of unfolded lysozyme are much more sensitive to orientation.
Resonance features in THz spectra of unfolded lysozyme are stronger and differ in frequencies from those of native lysozyme.
The sensitivity of THz spectroscopy is high enough to discriminate between unfolded samples prepared by different techniques reflecting different degree of unfolding process.
THz spectroscopy apparently can be used for monitoring folding-unfolding process of proteins.
Globus, UVA, June 2005, JNL
CC892-F, bl-a
Frequency,cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.54
0.56
0.58
0.60
0.62 0 deg. 0 deg., repeat60 deg. 60 degr., repeat
Typical transmission spectra of cancer cells.Resonance structure is sensitive to orientation of a sample relative to the electric field of radiation.
E
H
Experimental Results on Cancer Cells
Long term reproducibility of transmission spectra of prostate cancer cells measured at the same orientationafter storage of the sample in frozen condition for several weeks.
Radiation is 80% polarized
CC896, pr-b
Frequency, cm-1
10 12 14 16 18 20 22 24
Tran
smis
sion
0.40
0.42
0.44
0.46
0.48
0.50 (1) 10-22-03(2) 10-22-03 (3) 10-30-03 (4) 11-13-03
Globus, UVA, June 2005, JNL
Prostate and Bladder Cancer Cells
Liquid and frozen samples measured at close orientation have a similar pattern.
Structure around 14 cm-1 have been chosen to control material orientation, and spectra from different samples having the same orientation have been compared using the structure at 14 cm-1 as a key.
a) Cancer Cells frozen
Frequency, cm-1
10 12 14 16 18 20 22 24Tr
ansm
issi
on
0.44
0.46
0.48
0.50
0.52
0.54
CC900, 0 deg., bl-b CC888, 0 deg., pr-a
Spectra of prostate and bladder cancer cells
10 12 14 16 18 20 22 24
Tran
smis
sion
0.38
0.40
0.42
0.44
0.46
0.48
0.50
CC896, frozen CC896
CC895
Frequency, cm-1
0.24
0.26
0.28
0.30
0.32
0.34
0.36
CC895, liquid
There are noticeable difference in spectra of prostate and bladder cancer cells:
Globus, UVA, June 2005, JNL
Cancer cells revealed vibrational modes in THz transmission spectra with theresonance structure that is sensitive to the orientation of cells relative to the electromagnetic field of radiation.
Spectral features, like position of transmission minima which correspond to themaxima of absorption, demonstrated good long-term reproducibility and hadalmost the same pattern after storage of samples for 2 weeks at 0° C or below when sample orientation was maintained a constant.
Comparison of transmission spectra for a prostate and a bladder cancer cell lines measured at the “same orientation” revealed spectral features which can be used to discriminate between them.
THz spectroscopy appears to be a promising approach toward the characterization of human tumor histology, and with further refinement, may be capable of discriminating between different tumor phenotypes and biologicpotential.
CONCLUSIONS
Globus, UVA, June 2005, JNL
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D. L. Woolard, T. R. Globus, B. L. Gelmont, M. Bykhovskaia, A. C. Samuels, D. Cookmeyer, J. L. Hesler, T. W. Crowe, J. O. Jensen, J. L. Jensen and W.R. Loerop. “Submillimeter-Wave Phonon Modes in DNA Macromolecules", Phys. Rev E. 65, 051903 (2002)
T. Globus, M. Bykhovskaia, D. Woolard, and B. Gelmont , “Sub-millimeter wave absorption spectra of artificial RNA molecules”, Journal of Physics D: Applied Physics 36, 1314-1322 (2003).
T. R. Globus, D. Woolard, M. Bykhovskaia, B. Gelmont, L. Werbos and A Samuels, “THz Spectroscopic Sensing of DNA and Related Biological Materials” in Terahertz Sensing Technology, Volume II, World Scientific Publishing, International Journal of High SpeedElectronics and Systems (IJHSES), 13, No4, Dec. 2003.
T. Globus, M. Bykhovskaia, D. Woolard, and B. Gelmont , “Sub-millimeter wave absorption spectra of artificial RNA molecules ,”Journal of Physics D: Applied Physics 36, 1314-1322 (2003).
T. R. Globus, D. L. Woolard, T. Khromova, T. W. Crowe, M. Bykhovskaia, B. L. Gelmont,. Hesler, and A. C. Samuels, “Thz-Spectroscopy of Biological Molecules,” Journal of Biological Physics, V29, 89-100 (2003).
T. Globus, T. Khromova, D. Woolard and B. Gelmont, “Terahertz Fourier transform characterization of biological materials in solid and liquid phases”, Chemical and Biological Standoff Detection in the Terahertz Region, Providence, RI, Oct 2003, Proceedings of SPIE Vol. 5268-2, pp.10-18 (2004).
M. Bykhovskaia, B. Gelmont, T. Globus, D.L.Woolard, A. C. Samuels, T. Ha-Duong, and K. Zakrzewska, “Prediction of DNA Far IR Absorption Spectra Basing on Normal Mode Analysis”, Theoretical Chemistry Accounts 106, 22-27 (2001).
T. M. Korter and D. F. Plusquellic, Continuous-wave terahertz spectroscopy of biotin: vibrational anharmonicity in the far-infrared, Chemical Physics Letters, Volume 385, Issues 1-2, Pages 45-51 (2 February 2004).
E.R. Brown, J.E. Bjarnason, T.L.J. Chan, A.W.M. Lee, and M.A. Celis, Optical Attenuation Signatures of Bacillus Subtilis in the THz Region, Appl. Phys Letters, 84, pp. 3438-3440 (2004).
T. Globus, R. Parthasarathy, T. Khromova, D. Woolard, N.Swami, A. J. Gatesman, J. Waldman, “Optical characteristics of biological molecules in the terahertz gap”, Proceedings of SPIE Vol. 5584-1 “Chemical and Biological Standoff Detection II” OE04 Optics East, Phyladelphia, Oct. 2004.
T. Globus, D. L. Woolard, A.C. Samuels, T. Khromova, J. O. Jensen “Sub-Millimeter Wave Fourier Transform Characterisation of Bacterial Spores”. Proceedings of the International Symposium on Spectral Sensing Research, Santa Barbara, California, 2-6 June 2003, file://F:\isssr2003\index.htm (2004).
T. Globus, D. Theodorescu, H. Frierson , T. Kchromova , D. Woolard, Terahertz spectroscopic characterization of cancer cells, SPIE-The International Society for Optical Engineering Conference: the Advanced Biomedical and Clinical Diagnostic Systems III, v. 5692-42, San Jose, 2005
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