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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Thomas Waeghe, Ph.D. August 16, 2005
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Thomas Waeghe, Ph.D. August 16, 2005
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Thomas Waeghe, Ph.D. August 16, 2005
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Presenter: Thomas Waeghe, Ph.D.
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choosing Columns and Conditions for LC/MS
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Atmospheric Pressure Ionization (API) LC/MS Techniques
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Relative Applicability of Atmospheric Pressure Ionization Techniques
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Definition and Application of Electrospray Technique
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
ESI Process
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 10
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Key Chromatographic Points in Use of Electrospray
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Definition and Application of APCI Technique
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
APCI and APPI Sources for the LC/MSD
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
AP Chemical Ionization (APCI) Process
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 15
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Key Chromatographic Points in Use of APCI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Definition and Application of APPI Atmospheric Photoionization
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
APPI Mechanisms
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
APPI Process
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Atmospheric Pressure Ionization Techniques
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 21
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Selecting Columns and Conditions for LC/MS
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Agilent LC/MS Column Configurations
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Longer Columns for Complex Samples
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Longer Columns for Maximum Chromatographic Resolution 2.1 x 150 mm Column
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Shorter Columns for Fast Analysis with MS Identification 2.1 x 50 mm Column
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Short Columns with Sub Two-Micron Particles for Rapid LC/MS APCI Analysis ofClindamycin and...
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Mass Spectra of Clindamycin and Lincomycin High Throughput LC/MS with Rapid Resolution HTColumn
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Solvent Saver 3.0 mm I.D. is Ideal for Converting from LC to LC/MS
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Capillary Columns for LC/MS of Proteins and Peptides Identifiy Peptide PhosphorylationSite...
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Protein Separation on a NanoHPLC Column with Nano-electrospray MS Detection
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Break Number 2
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Mobile Phase Selection - ESI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choosing a Volatile Mobile Phase Comparison of Results with Volatile and Non-volatile Mobile PhaseA...
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Comparison Between Volatile Buffer and Non Volatile Phosphate Buffer (pH 2.5) (10 ppminjection:sc...
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Adjust Mobile Phase pH to Ionize Molecules in Solution ESI - LC/MS of Bases and Acids
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Basic Compounds - Benzodiazepines
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Choose Low pH Mobile Phase for Ionized Basic Compounds Positive Ion ESI LC/MS ofBenzodiazepines
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Mobile Phase Buffers for ESI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Solvent Selection and Guidelines for Successful APCI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Carbamates by APCI: Extracted Ions
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Electrospray Ionization (ESI) vs. APCI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Solvent Selection and Guidelines for Successful APPI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Effect of Chromatographic Solvent on Photoionization of Fat-Soluble Vitamins
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Effect of Dopants on Photoionization of Fat-Soluble Vitamins
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Analysis of Tocopherols (Vitamin E Isomers) by APPI
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Good Chromatography Always Requires Selecting the Best Bonded Phase
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Bonded Phase Selection at Low pH
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Bonded-Phase Selection at Mid pH
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
LC/MS Bonded Phase Selection at High pH – Extend-C18
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Bonded Phase and Mobile Phase Choices for LC/MS Analysis
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Adapting Existing LC Methods to LC/API-MS
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Conclusions
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
HPLC Column Technical Support
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 55
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Choosing a Column and Conditions for LC-MS - 081605 - Tom Waeghe
Slide 56
