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Introducing Thermo Scientific
Pierce Prestained and Unstained
Protein Ladders for easy protein
molecular weight determination.
Fermentas
now sold as
Thermo Scientific
Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe SuperSignal Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.
Electrophoresis Gel Stains GuideThis 21-page brochure summarizes the key features and benefits of Thermo Scientific Pierce Staining Products. Easy-to-follow protocols with schematics demonstrate just how easy staining can be. To download this technical handbook, visit www.thermoscientific.com/pierce
5µL 10µL 5µL 10µL
150kDa
100kDa
80kDa
60kDa
50kDa
40kDa
30kDa
20kDa
4-12% Bis-Tris 4-20% Tris-Glycine
Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific Pierce Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.
Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific PageRuler Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin conjugates.
Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific PageRuler and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.
Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific Spectra Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.
with new protein ladder choices.Reach greater highs (and lows)
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDaPageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
© 2011 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Novex and NuPAGE are registered trademarks of Invitrogen. Ready Gel is a registered trademark of Bio Rad. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
BN
0307
116
03/
11 P
rinte
d in
the
U.S
.
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDaPageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
Product #’s PI-84785, PI-84786
Introducing Thermo Scientific
Pierce Prestained and Unstained
Protein Ladders for easy protein
molecular weight determination.
Fermentas
now sold as
Thermo Scientific
Thermo Scientific Pierce Protein Research Product Portfolio now includes protein ladders made with Fermentas technology. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our unstained protein ladders.
Highlights:
• Cost effective – high quality without the high price; average cost of less than $1 per gel application
• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination
• Convenient – protein ladders are ready to load with no heating or boiling required
• Reliable – exceptional lot-to-lot consistency and reproducibility
Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe SuperSignal Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.
Electrophoresis Gel Stains GuideThis 21-page brochure summarizes the key features and benefits of Thermo Scientific Pierce Staining Products. Easy-to-follow protocols with schematics demonstrate just how easy staining can be. To download this technical handbook, visit www.thermoscientific.com/pierce
5µL 10µL 5µL 10µL
150kDa
100kDa
80kDa
60kDa
50kDa
40kDa
30kDa
20kDa
4-12% Bis-Tris 4-20% Tris-Glycine
Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific Pierce Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.
Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific PageRuler Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin conjugates.
Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific PageRuler and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.
Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific Spectra Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.
with new protein ladder choices. Reach greater highs (and lows)
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDaPageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
© 2011 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Novex and NuPAGE are registered trademarks of Invitrogen. Ready Gel is a registered trademark of Bio Rad. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
BN
0307
116
03/
11 P
rinte
d in
the
U.S
.
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDaPageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
Product #’s PI-84785, PI-84786
Reach new heights in gel electrophoresis
0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
Spectra Multicolor High Range
Spectra Multicolor Broad Range
PageRuler Unstained Broad Range
PageRuler Plus Prestained Ladder
PageRuler Unstained Ladder
PageRuler Prestained Ladder
Pierce Prestained Marker
Pierce Unstained Marker
PageRuler Unstained Low Range
Spectra Multicolor Low Range
Protein Ladder Range (kDa)
The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.
Unstained Ladder
Prestained Ladder
Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders
Rem
ove
this
pan
el a
t per
f and
reta
in fo
r fut
ure
refe
renc
e.
Reference Guide: Migration Patterns in Different Electrophoresis Conditions
Mig
ratio
n Pa
ttern
s of
Spe
ctra
Mul
ticol
or
Hig
h Ra
nge
and
Low
Ran
ge P
rote
in L
adde
rM
igra
tion
Patte
rns
of S
pect
ra M
ultic
olor
B
road
Ran
ge P
rote
in L
adde
rM
igra
tion
Patte
rns
of P
ageR
uler
Pr
esta
ined
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Pag
eRul
er P
lus
Pres
tain
ed P
rote
in L
adde
r
SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex® precast gels.††† – migration patterns were determined using respective Ready Gel® precast gel.
% le
ngth
of g
el 40
25
15
1052
40
25
15
1052
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
52
40
25
15
10
52
40
25
15
1052
40
25
15
105
40
15
1052
Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine
Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †
Running buffer Tris-Tricine SDS
Apparent Molecular Weights, kDa
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
300250
180
130
100
70
5040
300
250
180
130
100
300250180
130
100
70
50
40
300
250
180
130
100
70
5040
270205
150
120
85
65
50
40
270205150120
85
65
50
40
270185140115
80
65
50
40
300250180
130
100
70
50
40
300250180130100
70
50
40
Thermo Scientific Spectra Multicolor High Range Protein LadderGel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
260
140100
70
504035
25
15
10
260
14010070
50
40
35
25
15
10
260
140
100
70
50
40
35
25
15
260
140100
70
50
40
35
25
15
26014010070
50
4035
25
15
10
26014010070
50
40
35
25
15
10
225
120
85
65
40
50
3025
225
120
85
65
40
50
30
25
225
115
80
65
35
50
30
25
1510
225
11580
65
35
50
30
25
15
225
1158065
35
50
30
25
15
235
120
8070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
235
1208070
40
50
3025
15
10
260
140
100
70
50
4035
25
15
10
Thermo Scientific Spectra Multicolor Broad Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
205
120
85
65
30
50
25
250
100
70
35
55
25
15
130
19011580
70
30
50
25
15
10
190
1158070
30
50
25
15
10
185
115
80
65
30
50
25
1510
250
100
70
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
100
70
35
55
25
15
130
250
10070
35
55
25
130
10
250
10070
35
55
25
15
130
10
205
120
85
65
30
50
25
185
1158065
30
50
25
15
185
1158065
30
50
25
15
1901158070
30
50
25
15
10
% le
ngth
of g
el
15
Thermo Scientific PageRuler Plus Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
17013010070
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
17013010070
55
4035
25
15
10
170130
100
70
55
40
35
25
15
170130100
70
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
150
120
85
65
50
403025
150
120
85
65
50
40
30
25
115140
80
65
50
40
30
25
15
10
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
10
1401158070
50
40
30
25
15
10
1401158070
5040
30
25
15
10
Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
†† – Migration patterns were determined using respective NuPAGE® precast gels.
% le
ngth
of g
el
Thermo Scientific PageRuler Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
Ordering Information
Product # DescriptionPkg. Size
PI-26610 Pierce® Unstained Protein MW Marker
2 x 1mL
PI-26611 Pierce Unstained Protein MW Marker
25µL
PI-26612 Pierce Prestained Protein MW Marker
500µL
PI-26613 Pierce Prestained Protein MW Marker
25µL
PI-26614 PageRuler Unstained Protein Ladder
2 x 250µL
PI-26615 PageRuler Unstained Protein Ladder
25µL
PI-26616 PageRuler Prestained Protein Ladder
2 x 250µL
PI-26617 PageRuler Prestained Protein Ladder
10 x 250µL
PI-26618 PageRuler Prestained Protein Ladder
25µL
PI-26619 PageRuler Plus Prestained Protein Ladder
2 x 250µL
PI-26620 PageRuler Plus Prestained Protein Ladder
10 x 250µL
PI-26621 PageRuler Plus Prestained Protein Ladder
25µL
PI-26634 Spectra™ Multicolor Broad Range Protein Ladder
2 x 250µL
PI-26623 Spectra Multicolor Broad Range Protein Ladder
10 x 250µL
PI-26624 Spectra Multicolor Broad Range Protein Ladder
50µL
PI-26625 Spectra Multicolor High Range Protein Ladder
2 x 250µL
PI-26626 Spectra Multicolor High Range Protein Ladder
50µL
PI-26628 Spectra Multicolor Low Range Protein Ladder
250µL
PI-26629 Spectra Multicolor Low Range Protein Ladder
25µL
PI-26630 PageRuler Unstained Broad Range Protein Ladder
2 x 250µL
PI-26631 PageRuler Unstained Broad Range Protein Ladder
25µL
PI-26632 PageRuler Unstained Low Range Protein Ladder
2 x 250µL
PI-26633 PageRuler Unstained Low Range Protein Ladder
25µL
PI-84785 SuperSignal Molecular Weight Protein Ladder
250µL
PI-84786 SuperSignal Enhanced Molecular Weight Protein Ladder
250µL
Application
Thermo Scientific Pierce Protein Ladders SDS PAGE Western blotting
Product Name Product #
Former Fermentas Part # # Proteins
MW Range
Reference Bands
PageRuler Unstained Broad Range Protein Ladder
PI-26630 SM1881 11 5-250kDa 20, 50 and 100kDa
Best — — Best — — Good†
PageRuler Unstained Low Range Protein Ladder
PI-26632 SM1891 8 3.4-100kDa 25kDa Best — — Best — — Good†
PageRuler Unstained Protein Ladder
PI-26614 SM0661 14 10-200kDa 50kDa Good — — Good — — Good†
Pierce Unstained Protein Molecular Weight Marker
PI-26610 SM0431 7 14.4-116kDa
— Good — — Good — — —
SuperSignal Molecular Weight Protein Ladder
PI-84785, PI-84786
— 8 20-150kDa — Good — — Good — Good Best
PageRuler Prestained Protein Ladder
PI-26616, PI-26617
SM0671, SM0672
10 10-170kDa Green 10kDa; Orange 70kDa
Good Good Good — Good‡ Good —
PageRuler Plus Prestained Protein Ladder
PI-26619, PI-26620
SM1811, SM1812
9 10-250kDa Green 10kDa; Orange 25 and 70kDa
Good Good Good — Good‡ Good —
Spectra Multicolor Broad Range Protein Ladder
PI-26634, PI-26623
SM1841, SM1842
10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa
Good Best Best — Good‡ Best —
Spectra Multicolor Low Range Protein Ladder
PI-26628 SM1861 6 1.7-40kDa Green 10kDa; Orange 40kDa
Good Best Best — Good‡ Best —
Spectra Multicolor High Range Protein Ladder
PI-26625 SM1851 8 40-300kDa Green 50kDa; Orange 70 and 300kDa
Good Best Best — Good‡ Best —
Pierce Prestained Protein Molecular Weight Marker
PI-26612 SM0441 6 20-120kDa — Good Good Good — Good Good —
† with Strep-Tactin® HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region
Uns
tain
ed p
rote
in la
dder
sPr
esta
ined
pro
tein
ladd
ers
Prot
ein
MW
Dete
rmin
atio
nPr
otei
n
band
visu
aliza
tion
Mon
itorin
g
elec
troph
ores
is
run Co
omas
sie/
Silv
er/
Fluo
resc
ent
Stai
ning
NIR
de
tect
ion
Mon
itorin
g pr
otei
n
trans
fer
Chem
ilum
ines
cent
band
visu
aliza
tion
Reach new heights in gel electrophoresis
0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
Spectra Multicolor High Range
Spectra Multicolor Broad Range
PageRuler Unstained Broad Range
PageRuler Plus Prestained Ladder
PageRuler Unstained Ladder
PageRuler Prestained Ladder
Pierce Prestained Marker
Pierce Unstained Marker
PageRuler Unstained Low Range
Spectra Multicolor Low Range
Protein Ladder Range (kDa)
The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.
Unstained Ladder
Prestained Ladder
Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders
Rem
ove
this
pan
el a
t per
f and
reta
in fo
r fut
ure
refe
renc
e.
Reference Guide: Migration Patterns in Different Electrophoresis Conditions
Mig
ratio
n Pa
ttern
s of
Spe
ctra
Mul
ticol
or
Hig
h Ra
nge
and
Low
Ran
ge P
rote
in L
adde
rM
igra
tion
Patte
rns
of S
pect
ra M
ultic
olor
B
road
Ran
ge P
rote
in L
adde
rM
igra
tion
Patte
rns
of P
ageR
uler
Pr
esta
ined
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Pag
eRul
er P
lus
Pres
tain
ed P
rote
in L
adde
r
SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex® precast gels.††† – migration patterns were determined using respective Ready Gel® precast gel.
% le
ngth
of g
el 40
25
15
1052
40
25
15
1052
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
52
40
25
15
10
52
40
25
15
1052
40
25
15
105
40
15
1052
Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine
Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †
Running buffer Tris-Tricine SDS
Apparent Molecular Weights, kDa
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el300250
180
130
100
70
5040
300
250
180
130
100
300250180
130
100
70
50
40
300
250
180
130
100
70
5040
270205
150
120
85
65
50
40
270205150120
85
65
50
40
270185140115
80
65
50
40
300250180
130
100
70
50
40
300250180130100
70
50
40
Thermo Scientific Spectra Multicolor High Range Protein LadderGel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
260
140100
70
504035
25
15
10
260
14010070
50
40
35
25
15
10
260
140
100
70
50
40
35
25
15
260
140100
70
50
40
35
25
15
26014010070
50
4035
25
15
10
26014010070
50
40
35
25
15
10
225
120
85
65
40
50
3025
225
120
85
65
40
50
30
25
225
115
80
65
35
50
30
25
1510
225
11580
65
35
50
30
25
15
225
1158065
35
50
30
25
15
235
120
8070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
235
1208070
40
50
3025
15
10
260
140
100
70
50
4035
25
15
10
Thermo Scientific Spectra Multicolor Broad Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
205
120
85
65
30
50
25
250
100
70
35
55
25
15
130
19011580
70
30
50
25
15
10
190
1158070
30
50
25
15
10
185
115
80
65
30
50
25
1510
250
100
70
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
100
70
35
55
25
15
130
250
10070
35
55
25
130
10
250
10070
35
55
25
15
130
10
205
120
85
65
30
50
25
185
1158065
30
50
25
15
185
1158065
30
50
25
15
1901158070
30
50
25
15
10
% le
ngth
of g
el
15
Thermo Scientific PageRuler Plus Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
17013010070
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
17013010070
55
4035
25
15
10
170130
100
70
55
40
35
25
15
170130100
70
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
150
120
85
65
50
403025
150
120
85
65
50
40
30
25
115140
80
65
50
40
30
25
15
10
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
10
1401158070
50
40
30
25
15
10
1401158070
5040
30
25
15
10
Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
†† – Migration patterns were determined using respective NuPAGE® precast gels.
% le
ngth
of g
el
Thermo Scientific PageRuler Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
Ordering Information
Product # DescriptionPkg. Size
PI-26610 Pierce® Unstained Protein MW Marker
2 x 1mL
PI-26611 Pierce Unstained Protein MW Marker
25µL
PI-26612 Pierce Prestained Protein MW Marker
500µL
PI-26613 Pierce Prestained Protein MW Marker
25µL
PI-26614 PageRuler Unstained Protein Ladder
2 x 250µL
PI-26615 PageRuler Unstained Protein Ladder
25µL
PI-26616 PageRuler Prestained Protein Ladder
2 x 250µL
PI-26617 PageRuler Prestained Protein Ladder
10 x 250µL
PI-26618 PageRuler Prestained Protein Ladder
25µL
PI-26619 PageRuler Plus Prestained Protein Ladder
2 x 250µL
PI-26620 PageRuler Plus Prestained Protein Ladder
10 x 250µL
PI-26621 PageRuler Plus Prestained Protein Ladder
25µL
PI-26634 Spectra™ Multicolor Broad Range Protein Ladder
2 x 250µL
PI-26623 Spectra Multicolor Broad Range Protein Ladder
10 x 250µL
PI-26624 Spectra Multicolor Broad Range Protein Ladder
50µL
PI-26625 Spectra Multicolor High Range Protein Ladder
2 x 250µL
PI-26626 Spectra Multicolor High Range Protein Ladder
50µL
PI-26628 Spectra Multicolor Low Range Protein Ladder
250µL
PI-26629 Spectra Multicolor Low Range Protein Ladder
25µL
PI-26630 PageRuler Unstained Broad Range Protein Ladder
2 x 250µL
PI-26631 PageRuler Unstained Broad Range Protein Ladder
25µL
PI-26632 PageRuler Unstained Low Range Protein Ladder
2 x 250µL
PI-26633 PageRuler Unstained Low Range Protein Ladder
25µL
PI-84785 SuperSignal Molecular Weight Protein Ladder
250µL
PI-84786 SuperSignal Enhanced Molecular Weight Protein Ladder
250µL
Application
Thermo Scientific Pierce Protein Ladders SDS PAGE Western blotting
Product Name Product #
Former Fermentas Part # # Proteins
MW Range
Reference Bands
PageRuler Unstained Broad Range Protein Ladder
PI-26630 SM1881 11 5-250kDa 20, 50 and 100kDa
Best — — Best — — Good†
PageRuler Unstained Low Range Protein Ladder
PI-26632 SM1891 8 3.4-100kDa 25kDa Best — — Best — — Good†
PageRuler Unstained Protein Ladder
PI-26614 SM0661 14 10-200kDa 50kDa Good — — Good — — Good†
Pierce Unstained Protein Molecular Weight Marker
PI-26610 SM0431 7 14.4-116kDa
— Good — — Good — — —
SuperSignal Molecular Weight Protein Ladder
PI-84785, PI-84786
— 8 20-150kDa — Good — — Good — Good Best
PageRuler Prestained Protein Ladder
PI-26616, PI-26617
SM0671, SM0672
10 10-170kDa Green 10kDa; Orange 70kDa
Good Good Good — Good‡ Good —
PageRuler Plus Prestained Protein Ladder
PI-26619, PI-26620
SM1811, SM1812
9 10-250kDa Green 10kDa; Orange 25 and 70kDa
Good Good Good — Good‡ Good —
Spectra Multicolor Broad Range Protein Ladder
PI-26634, PI-26623
SM1841, SM1842
10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa
Good Best Best — Good‡ Best —
Spectra Multicolor Low Range Protein Ladder
PI-26628 SM1861 6 1.7-40kDa Green 10kDa; Orange 40kDa
Good Best Best — Good‡ Best —
Spectra Multicolor High Range Protein Ladder
PI-26625 SM1851 8 40-300kDa Green 50kDa; Orange 70 and 300kDa
Good Best Best — Good‡ Best —
Pierce Prestained Protein Molecular Weight Marker
PI-26612 SM0441 6 20-120kDa — Good Good Good — Good Good —
† with Strep-Tactin® HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region
Uns
tain
ed p
rote
in la
dder
sPr
esta
ined
pro
tein
ladd
ers
Prot
ein
MW
Dete
rmin
atio
nPr
otei
n
band
visu
aliza
tion
Mon
itorin
g
elec
troph
ores
is
run Co
omas
sie/
Silv
er/
Fluo
resc
ent
Stai
ning
NIR
de
tect
ion
Mon
itorin
g pr
otei
n
trans
fer
Chem
ilum
ines
cent
band
visu
aliza
tion
Reach new heights in gel electrophoresis
0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
Spectra Multicolor High Range
Spectra Multicolor Broad Range
PageRuler Unstained Broad Range
PageRuler Plus Prestained Ladder
PageRuler Unstained Ladder
PageRuler Prestained Ladder
Pierce Prestained Marker
Pierce Unstained Marker
PageRuler Unstained Low Range
Spectra Multicolor Low Range
Protein Ladder Range (kDa)
The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.
Unstained Ladder
Prestained Ladder
Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders
Rem
ove
this
pan
el a
t per
f and
reta
in fo
r fut
ure
refe
renc
e.
Reference Guide: Migration Patterns in Different Electrophoresis ConditionsM
igra
tion
Patte
rns
of S
pect
ra M
ultic
olor
H
igh
Rang
e an
d Lo
w R
ange
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Spe
ctra
Mul
ticol
or
Bro
ad R
ange
Pro
tein
Lad
der
Mig
ratio
n Pa
ttern
s of
Pag
eRul
er
Pres
tain
ed P
rote
in L
adde
rM
igra
tion
Patte
rns
of P
ageR
uler
Plu
s Pr
esta
ined
Pro
tein
Lad
der
SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex® precast gels.††† – migration patterns were determined using respective Ready Gel® precast gel.
% le
ngth
of g
el 40
25
15
1052
40
25
15
1052
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
5
2
40
25
15
10
5
40
25
15
10
52
40
25
15
10
52
40
25
15
1052
40
25
15
105
40
15
1052
Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine
Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †
Running buffer Tris-Tricine SDS
Apparent Molecular Weights, kDa
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
300250
180
130
100
70
5040
300
250
180
130
100
300250180
130
100
70
50
40
300
250
180
130
100
70
5040
270205
150
120
85
65
50
40
270205150120
85
65
50
40
270185140115
80
65
50
40
300250180
130
100
70
50
40
300250180130100
70
50
40
Thermo Scientific Spectra Multicolor High Range Protein LadderGel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
% le
ngth
of g
el
260
140100
70
504035
25
15
10
260
14010070
50
40
35
25
15
10
260
140
100
70
50
40
35
25
15
260
140100
70
50
40
35
25
15
26014010070
50
4035
25
15
10
26014010070
50
40
35
25
15
10
225
120
85
65
40
50
3025
225
120
85
65
40
50
30
25
225
115
80
65
35
50
30
25
1510
225
11580
65
35
50
30
25
15
225
1158065
35
50
30
25
15
235
120
8070
40
50
30
25
15
10
235
1208070
40
50
30
25
15
10
235
1208070
40
50
3025
15
10
260
140
100
70
50
4035
25
15
10
Thermo Scientific Spectra Multicolor Broad Range Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
205
120
85
65
30
50
25
250
100
70
35
55
25
15
130
19011580
70
30
50
25
15
10
190
1158070
30
50
25
15
10
185
115
80
65
30
50
25
1510
250
100
70
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
10070
35
55
25
15
10
130
250
100
70
35
55
25
15
130
250
10070
35
55
25
130
10
250
10070
35
55
25
15
130
10
205
120
85
65
30
50
25
185
1158065
30
50
25
15
185
1158065
30
50
25
15
1901158070
30
50
25
15
10
% le
ngth
of g
el
15
Thermo Scientific PageRuler Plus Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
17013010070
55
40
35
25
15
10
170
130
100
70
55
40
35
25
15
10
17013010070
55
4035
25
15
10
170130
100
70
55
40
35
25
15
170130100
70
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
17013010070
55
40
35
25
15
10
150
120
85
65
50
403025
150
120
85
65
50
40
30
25
115140
80
65
50
40
30
25
15
10
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
1401158065
50
40
30
25
15
10
1401158070
50
40
30
25
15
10
1401158070
5040
30
25
15
10
Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.
†† – Migration patterns were determined using respective NuPAGE® precast gels.
% le
ngth
of g
el
Thermo Scientific PageRuler Prestained Protein Ladder
Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††
Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%
Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES
Apparent Molecular Sizes (kDa)
10
20
30
40
50
60
70
80
90
100
Ordering Information
Product # DescriptionPkg. Size
PI-26610 Pierce® Unstained Protein MW Marker
2 x 1mL
PI-26611 Pierce Unstained Protein MW Marker
25µL
PI-26612 Pierce Prestained Protein MW Marker
500µL
PI-26613 Pierce Prestained Protein MW Marker
25µL
PI-26614 PageRuler Unstained Protein Ladder
2 x 250µL
PI-26615 PageRuler Unstained Protein Ladder
25µL
PI-26616 PageRuler Prestained Protein Ladder
2 x 250µL
PI-26617 PageRuler Prestained Protein Ladder
10 x 250µL
PI-26618 PageRuler Prestained Protein Ladder
25µL
PI-26619 PageRuler Plus Prestained Protein Ladder
2 x 250µL
PI-26620 PageRuler Plus Prestained Protein Ladder
10 x 250µL
PI-26621 PageRuler Plus Prestained Protein Ladder
25µL
PI-26634 Spectra™ Multicolor Broad Range Protein Ladder
2 x 250µL
PI-26623 Spectra Multicolor Broad Range Protein Ladder
10 x 250µL
PI-26624 Spectra Multicolor Broad Range Protein Ladder
50µL
PI-26625 Spectra Multicolor High Range Protein Ladder
2 x 250µL
PI-26626 Spectra Multicolor High Range Protein Ladder
50µL
PI-26628 Spectra Multicolor Low Range Protein Ladder
250µL
PI-26629 Spectra Multicolor Low Range Protein Ladder
25µL
PI-26630 PageRuler Unstained Broad Range Protein Ladder
2 x 250µL
PI-26631 PageRuler Unstained Broad Range Protein Ladder
25µL
PI-26632 PageRuler Unstained Low Range Protein Ladder
2 x 250µL
PI-26633 PageRuler Unstained Low Range Protein Ladder
25µL
PI-84785 SuperSignal Molecular Weight Protein Ladder
250µL
PI-84786 SuperSignal Enhanced Molecular Weight Protein Ladder
250µL
Application
Thermo Scientific Pierce Protein Ladders SDS PAGE Western blotting
Product Name Product #
Former Fermentas Part # # Proteins
MW Range
Reference Bands
PageRuler Unstained Broad Range Protein Ladder
PI-26630 SM1881 11 5-250kDa 20, 50 and 100kDa
Best — — Best — — Good†
PageRuler Unstained Low Range Protein Ladder
PI-26632 SM1891 8 3.4-100kDa 25kDa Best — — Best — — Good†
PageRuler Unstained Protein Ladder
PI-26614 SM0661 14 10-200kDa 50kDa Good — — Good — — Good†
Pierce Unstained Protein Molecular Weight Marker
PI-26610 SM0431 7 14.4-116kDa
— Good — — Good — — —
SuperSignal Molecular Weight Protein Ladder
PI-84785, PI-84786
— 8 20-150kDa — Good — — Good — Good Best
PageRuler Prestained Protein Ladder
PI-26616, PI-26617
SM0671, SM0672
10 10-170kDa Green 10kDa; Orange 70kDa
Good Good Good — Good‡ Good —
PageRuler Plus Prestained Protein Ladder
PI-26619, PI-26620
SM1811, SM1812
9 10-250kDa Green 10kDa; Orange 25 and 70kDa
Good Good Good — Good‡ Good —
Spectra Multicolor Broad Range Protein Ladder
PI-26634, PI-26623
SM1841, SM1842
10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa
Good Best Best — Good‡ Best —
Spectra Multicolor Low Range Protein Ladder
PI-26628 SM1861 6 1.7-40kDa Green 10kDa; Orange 40kDa
Good Best Best — Good‡ Best —
Spectra Multicolor High Range Protein Ladder
PI-26625 SM1851 8 40-300kDa Green 50kDa; Orange 70 and 300kDa
Good Best Best — Good‡ Best —
Pierce Prestained Protein Molecular Weight Marker
PI-26612 SM0441 6 20-120kDa — Good Good Good — Good Good —
† with Strep-Tactin® HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region
Uns
tain
ed p
rote
in la
dder
sPr
esta
ined
pro
tein
ladd
ers
Prot
ein
MW
Dete
rmin
atio
nPr
otei
n
band
visu
aliza
tion
Mon
itorin
g
elec
troph
ores
is
run Co
omas
sie/
Silv
er/
Fluo
resc
ent
Stai
ning
NIR
de
tect
ion
Mon
itorin
g pr
otei
n
trans
fer
Chem
ilum
ines
cent
band
visu
aliza
tion
Introducing Thermo Scientific
Pierce Prestained and Unstained
Protein Ladders for easy protein
molecular weight determination.
Fermentas
now sold as
Thermo Scientific
Thermo Scientific Pierce Protein Research Product Portfolio now includes protein ladders made with Fermentas technology. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our unstained protein ladders.
Highlights:
• Cost effective – high quality without the high price; average cost of less than $1 per gel application
• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination
• Convenient – protein ladders are ready to load with no heating or boiling required
• Reliable – exceptional lot-to-lot consistency and reproducibility
Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe SuperSignal Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.
Electrophoresis Gel Stains GuideThis 21-page brochure summarizes the key features and benefits of Thermo Scientific Pierce Staining Products. Easy-to-follow protocols with schematics demonstrate just how easy staining can be. To download this technical handbook, visit www.thermoscientific.com/pierce
5µL 10µL 5µL 10µL
150kDa
100kDa
80kDa
60kDa
50kDa
40kDa
30kDa
20kDa
4-12% Bis-Tris 4-20% Tris-Glycine
Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific Pierce Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.
Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific PageRuler Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin conjugates.
Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific PageRuler and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.
Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific Spectra Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.
with new protein ladder choices. Reach greater highs (and lows)
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDaPageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s 26612, 26613
4-12% Tris-glycine SDS-PAGE
Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE
Product #’s 26633, 266344-20% Tris-glycine SDS-PAGE
Product #’s 26630, 266318-16% Tris-glycine SDS-PAGE
Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE
Product # 26632
4-20% Tris-glycine SDS-PAGE
Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE
Product #’s 26619, 26620, 26621 8-16% Tris-glycine SDS-PAGE
Product #’s 26610, 26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s 26628, 26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
© 2011 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Novex and NuPAGE are registered trademarks of Invitrogen. Ready Gel is a registered trademark of Bio Rad. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
BN
0307
116
03/
11 P
rinte
d in
the
U.S
.
In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com
In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDaPageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
PageRuler PrestainedProtein Ladder
PageRuler PlusPrestained Protein Ladder
Prestained ProteinMolecular Weight Marker
Unstained ProteinMolecular Weight Marker
Blo
t
Gel
Blo
t
Gel
Protein
β-galactosidase
Bovine serum albumin
Ovalbumin
Lactate dehydrogenase
REase Bsp98I
β-lactoglobulinLysozyme
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26612, PI-26613
4-12% Tris-glycine SDS-PAGE
Product #’s PI-26625, PI-26626
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26633, PI-266344-20% Tris-glycine SDS-PAGE
Product #’s PI-26630, PI-26631
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26614, PI-26615
18% SP Tris-tricine SDS-PAGE
Product # PI-26632
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26616, PI-26617, PI-26618
4-20% Tris-glycine SDS-PAGE
Product #’s PI-26619, PI-26620, PI-26621
8-16% Tris-glycine SDS-PAGE
Product #’s PI-26610, PI-26611
Gel
Gel
Spectra MulticolorHigh Range Protein Ladder
Spectra MulticolorBroad Range Protein Ladder
PageRulerUnstained Protein Ladder
PageRuler Unstained Broad Range Protein Ladder
PageRuler Unstained Low Range Protein Ladder
Spectra MulticolorLow Range Protein Ladder
18% SP Tris-tricine SDS-PAGESP - Small Peptide
SDS - polyacrylamide gel with higher crosslinking, C=5%
Product #’s PI-26628, PI-26629
~260
~140
~100
~70
~50
~40~35
~25
~15
~10
kDa
–
–
–
–
–
––
–
–
–
–
–
–
–
–
––
–
–
–
~40~25
~15
~10
4.6
1.7
kDa
––
–
–
–
–
––
–
–
–
–
~120~ 85
~50
~35
~25
~20
––
–
–
–
–
kDa
116.0
66.2
45.0
35.0
25.0
18.414.4
–
–
–
–
–
––
kDa
~300~250
~180
~130
~100
~70
~50
~40
kDa
Gel
Blo
t
Gel
Blo
t
Gel
Gel
Gel
Gel
Blo
t
––
–
–
–
–
–
–
––
–
–
–
–
–
–
250150
100
705040
30
20
15105
––
–
–
––
–
–
–––
kDa
100
30 25 20 15
10
5
3.4
–
––––
–
–
–
kDa20015012010085706050
40
302520
15
10
––––––––
–
–––
–
–
kDa
~170
~130
~100
~70
~55
~40
~35
~25
~15
~10
kDa
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
–
~250
~130
~100
~70
~55
~35
~25
~15
~10
kDa
Product #’s PI-84785, PI-84786