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Thermo ScientificEvolution 300 and Evolution 600
UV-Vis Spectrophotometers
Life Science
Material Science
Pharmaceutical
m o l e c u l a r s p e c t r o s c o p y
Part of Thermo Fisher Scientific
2
Reliable, High-Performance UV-Visible Spectrophotometers
For over 60 years, customers in world-renowned research institutions, highly regulated QClaboratories and teaching institutions have relied on Thermo Scientific UV-Visible (UV-Vis)spectrophotometers. The Thermo Scientific Evolution 300/600 UV-Visible spectrophotometerseries is founded on the successful legacy of the Pye-Unicam, Bausch & Lomb, PhilipsScientific, and Spectronic products. Many customers today say they made their first absorbancemeasurement on a Thermo Scientific SPECTRONIC 20 spectrophotometer, a product on themarket since 1953. The Evolution™ 300/600 series continues in this tradition by deliveringthe solid, high-performance you need for your UV-Vis applications.
The PU8800, designed by Pye-Unicam inthe 1980’s, was one of the industry-leadingresearch instruments of its time.
Contents
Optical Design 4
System Configuration 6
Life Science Biochemical 8
Pharmaceutical 10and QC Analysis
Material Science 12
Smart Accessories 14
Thermo Scientific UV-VisSpectrophotometer Product LinesSPECTRONIC™ 15/20/20D+, SPECTRONICEducator™, and Thermo Scientific GENESYS 20
• Basic teaching• Easy sample and data handling
GENESYS™ 10S Series• Routine quantitative analysis and common bio assays• Easy-to-use accessories
Thermo Scientific Helios, Evolution 60S, and Evolution 160
• Routine quantitative analysis and bio assays• More advanced accessories and software
Evolution 300/600 Series• Wide range of accessories• Advanced quantitative analysis and bio assays• Extensive software offerings• Tablet dissolution• Material science
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Life Science
Material SciencePharmaceutical
QC
BiochemicalThe Evolution 300/600 spectrophotometer series incorporates a high-performance optical design, versatileThermo Scientific VISION software packages, and thehighest quality accessories for the most demandingapplications. The systems offer a multitude of advantagesfor a wide variety of applications including:
• Life Science Biochemical• Pharmaceutical and QC• Material Science
Innovative Smart Accessories™ offer ease of use andincreased productivity by providing:
• Snap-and-go installation• Seamless software integration• Serial number tracking for the regulated environment
System configuration choices offer the ultimate in flexibility for your laboratory. Choose from:
• Stand-alone Local Control version• PC Control version• Combination of both
Evolution 300 and Evolution 600 U
V-Vis Spectrophotometers
High-Performance Optical Design for Reliable Results
Maintaining high performance is the ongoing legacy you can count onwith the Thermo Scientific Evolution 300/600 spectrophotometer series.
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Mercury Lamp Primary referencestandard, allows
seamless validationand calibration of
the instrument
Light SourceXenon flash lamp
is only on when taking measurementsextending its lifetime
Optics Quartz overcoatedoptics for long-lasting reflectivity and cleaning withoutdamaging the optical surface
ChassisRigid, cast-aluminum base with opticalmounts bolted directly onto the base providessuperior system stability and reliable results
Detectors Silicon photodiodes forextended wavelengthrange to 1100 nm
Micrometer MonochromatorDrive with Sliding CouplingProvides excellent accuracy, reproducibility and minimizes temperature effects
Slit MechanismPhoto-etched slits for greater accuracy.Stepper-motor driven slit drive with intelligent optimization
Grating 1200 lines/mm, 240 nm blazed, holographic grating, for exceptional stray light performance
Evolution 300Optics
The Evolution 300 optical design includes a long lifetime xenon flash lamp and extended
wavelength-range silicon photodiode detectors. This system is an excellent choice for routine and
research laboratories in the Life Science and Pharmaceutical markets.
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Light SourcesDeuterium and
Halogen lamps foradded stability
when measuringminute absorbance
changes
MonochromatorBoth models feature
research grade, Ebert monochromator
for excellent energythroughput
Source OpticsAuto-aligningsource optics automatically
optimize throughputwhen the lamp is
changed
Smart SamplingPlug-in, instrument-
controlled accessoriesfor ease of use
and reliability
Detector High-performance,
red-sensitive photomultiplier
(PMT) detector fordemanding samples
ChopperFour-segment chopper runningat 33 Hz eliminates possibleoscillation conflicts with globalmains AC power frequencies of 60 or 50 Hz
Sample CompartmentLarge sample compartmentand wide beam separation
with interchangeable baseplates for full
sampling versatility
The optical design of the Evolution 600 includes deuterium and tungsten sources and a
high-performance photomultiplier detector. These features provide enhanced high-level
performance for the Material Science market and general research applications.
Evolution 600 Optics
Evolution 300 andEvolution 600 U
V-VisSpectrophotom
eters
Operational Flexibility to Fit Your Lab Needs
The Evolution 300/600 series offers the flexibility to choose a configuration suited to your needs: Local Control, PC software control,or Local Control with PC software control. Each of these configurationsoffers various levels of operational flexibility to fit the individual ormulti-user laboratory environment.
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Local Control The Local Control configuration offers complete instrument control, comprehensivemethod and data file manipulation, and savesvaluable bench space. It can also be pass-word protected preventing unwanted accessfrom outside your laboratory. For presentationpurposes, the Local Control screen displaycan be viewed on an external monitor orprojection system using the DVI output.
Local and PC ControlThe Local Control with PC software controlconfiguration gives you the freedom tochoose the configuration that matches yourexperimental needs. For example, use LocalControl to make a quick absorption meas-urement to determine the purity of a DNAsample. You do not need to wait for the PC toboot and the software to load. Later, to runa kinetics experiment based on a methodstored on a network drive, simply openVISION™ software, and switch the instrumentto PC control. This configuration is ideal forlabs with a variety of experimental needs.
PC ControlThe PC Control configuration allows users tocollect data and distribute it to third-partyapplications for advanced processing andarchival. In multi-user environments, LocalControl with PC software control gives youthe ultimate flexibility.
The PC Software Control configurationuses the powerful VISION suite of softwareto control the Evolution 300/600 instruments.This software allows you to collect, store,recall, and analyze your data, using oneconvenient program. From QC quantitativeanalysis measurements to complex enzymaticassays, the right VISION software packageis available for your application.
The large, color display and tactile keypadmake the Evolution Local Control system convenient and easy-to-use.
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Thermo Scientific SmartStart SmartStart™ allows each user to set up theSmartStart menu with the applications they usemost often. This increases the throughput of everyuser by eliminating the need to navigate menusand by decreasing the amount of time required totrain users. Load the SmartStart menu with anyapplication, including your stored methods, andwatch the productivity of your lab increase.
The standard VISIONpro software offers advanced scanning, multiple fixed wavelength measurements,and multi-wavelength measurements with customized UVcalc. Quantitative analysis and multicomponentanalysis applications are also included.
VISION Software SuiteThe Thermo Scientific VISIONpro is a flexiblesoftware package designed for the generalresearch and QC laboratory. When usedwith VISIONpro™, the Thermo ScientificVISIONlife software package adds the abilityto monitor and calculate reaction kinetics andto perform DNA melting experiments. Forthose laboratories requiring 21 CFR Part 11compliance, Thermo Scientific VISIONsecuritysets the standard for UV-Vis instrumentation.
Additional application software packages for the Evolution 300/600 UV-Visseries include: Thermo Scientific VISIONliteColorCalc for color measurements andEnzLab for Enzymatic Food Analysis.
VISION Software Application Comparison Chart
Software Scanning Fixed Abs/%T Quant Multicomponent Rate DNA
VISIONpro ✔ ✔ ✔ ✔
VISIONpro + VISIONlife™ ✔ ✔ ✔ ✔ ✔ ✔
VISIONsecurity™ ✔ ✔ ✔ ✔
VISIONsecurity + VISIONlife ✔ ✔ ✔ ✔ ✔ ✔
Customized ReportingThe Evolution advanced Report Composer inVISIONpro, VISIONlife and VISIONsecurity software allows you to precisely configure theformat of reports. These templates can be linked toan application method and used to automaticallyproduce reports in your customized format.
Nucleic Acid TestsThe Evolution series provides all the necessarytests for determining DNA purity in thepresence of protein or phenol. DNA/RNAconcentrations can be determined by simpleratios or by wavelength scanning. Thewavelength scanning option allows theadded flexibility of checking for potentialcontaminants.
Various quantization methods are available to determine the concentration ofpurified double-stranded and single-strandedDNA and RNA. Based on user-entered factors,the Evolution instrument measures theabsorbance of the DNA or RNA and calcu-lates the appropriate concentration.
The Oligo (Calc Factor) function allowsyou to enter a specific oligonucleotidesequence and from a subsequent absorbancemeasurement, the system calculates the%GC content, MW of the oligo, molar(pmol/ul) concentration of the sample andan estimate of the Tm value.
Evolution 300 and Evolution 600 U
V-Vis Spectrophotometers
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With no external circulating water required, the Evolution spectrophotometerwith the Smart Peltier accessory can reach a maximum temperature of 110 °C. This extended range provides added flexibility for making accurate Tm measurements on very long oligonucleotides.
Protein ConcentrationIf your research involves the determination ofprotein concentration assays, the EvolutionLocal Control systems provide the standardprotein assays at the push of a button.
Thermal Denaturation/RenaturationWhether your research examines short orlong DNA or RNA sequences, duplexes ortriplexes, the Evolution VISIONlife ThermalDenaturation/Renaturation system will meetall of your experimental needs. The systemprovides all the tools necessary to controlcomplex heating and cooling profiles withuser defined hold times and ramp ratesdown to 0.1 °C/min. Multiple built-in fittingalgorithms are available to calculate the Tm
value from all types of melting curves.
Comprehensive Solutions for Life Science Biochemical Applications
All of the major, industry-standard protein concentration assays at your fingertips.
The Evolution 300/600 series is the natural fit for any multi-user, life-scienceresearch laboratory. From users performing fast or routine assays, such asNucleic Acid tests, to users performing more advanced studies, such asMichaelis-Menton kinetics or Thermal Denaturation/Renaturation assays,these instruments offer a configuration ideal for your laboratory.
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KineticsBoth the Local Control and PC Control configurationscater to the user performing single or multi-celldouble-beam kinetics. The Local Control colorscreen makes it easy to distinguish the individualresults from multiple kinetics reactions. Up toseven active reference cuvettes can be used in aseven-sample/seven-reference configuration. Forlaboratories performing more complex assays, suchas inhibitor studies, VISIONlife software providescalculations for the Michaelis-Menton Km and Vmax
parameters using methods such as Lineweaver-Burke and Eadie-Hoftsee.
All of the routine biological assays, including abuilt-in Oligo calculator, are accessible in onlytwo keystrokes.
Quantitative Analysis Made SimpleThe Evolution Quant application is availablein both the Local Control and PC Controlconfigurations. For very quick and simplequant analysis in a routine QC laboratory,the Local Control configuration with built-in,soft-key menu operation is an excellentchoice. In a regulated environment, theEvolution series provides the tools toachieve 21 CFR Part 11 compliance.
The Quant application can use the datafrom up to 50 standards (3 replicates each)to analyze 999 samples (3 replicates each).VISIONpro software provides automatedweight and volume correction eliminatingadditional time-consuming calculations. Ifyour laboratory is performing analysis onmixtures, then the multicomponent analysisapplication, standard with VISIONpro, is ideal.
UVcalc for Automated UserCalculationsIf your analyses routinely demand automatedcalculations on experimental data, you will find the easy-to-use spreadsheet format of the UVcalc function beneficial.UVcalc allows you to enter and store the calculation formulas as you develop dataacquisition methods. When the method isrecalled, both the data acquisition and thecalculations are performed automatically,saving valuable time.
Evolution 300 and Evolution 600 U
V-Vis Spectrophotometers
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Complete Tools for Pharmaceutical and QC Analysis
Remote Fiber Optics MeasurementsA remote fiber optic probe allows the user to perform absorption measurements inside almost any vessel. A simplerinse between each measurement makes sample carryovernonexistent. Increase sample throughput by taking light to anysample container instead of wasting time bringing the sampleto the instrument in a cuvette or test tube.
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The US FDA Regulation 21 CFR Part 11describes the criteria under which the FDAwill consider electronic records equivalentto paper records and electronic signaturesequivalent to handwritten signatures. Thisregulation applies to all GxP manufacturersand industries that are FDA-regulated.
VISIONsecurity software provides allthe tools necessary to help your laboratoryachieve 21 CFR Part 11 compliance.
Whether compliance requires a manuallyinserted, traceable standard or fully automatedintelligent verification, the Thermo Scientificproprietary Smart Calibration ValidationCarousel (CVC) traceable to NIST1 or NPL2
standards fully automates testing for:• Wavelength accuracy• Photometric accuracy• Stray light• Photometric noise• Photometric stability• Baseline flatness
To obtain the best possible accuracy acrossthe entire wavelength range each instrumentis factory calibrated at multiple wavelengthsfrom the ultraviolet to the near-IR region usinga mercury lamp. An optional mercury lampaccessory is available and user installable.
1. National Institute of Standards and Technology, USA2. National Physical Laboratory, UK
Tablet DissolutionAn important application where complianceissues are critical is in the testing of soliddosage forms. Thermo Fisher Scientific andSOTAX have entered into a relationship toprovide semi-automated and fully automatedTablet Dissolution testing systems supportingApparatus I, II, IV, V and VI. The systemcombination provides a complete and seam-less solution for any dissolution laboratory.
Evolution 300 with the SOTAX Apparatus IV flow-through system offers a unique solution for avariety of Tablet Dissolution assays
With continually changing requirements to keepyour Evolution spectrophotometer up to regulatorystandards, no supplier in the industry can providea more comprehensive selection of calibrationand verification tools.
VISIONsecurity not only has all of the acquisition,analysis, and reporting capabilities found in theVISIONpro software, but also has the tools necessary to control individual or group access tothe instrument, data, and records. User authenti-cation at start-up allows event audit trails, electronic signatures, and prevents use while the operator is away.
By replacing paper-based archival storage withelectronic data storage and data transfer, theproper use of VISIONsecurity software with your Thermo Scientific spectrophotometer willhelp you pass FDA audits while reducing costsand increasing security.
Comprehensive Solutions for Regulatory Compliance
Evolution 300 and Evolution 600 U
V-Vis Spectrophotometers
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Long Path Diffuse Transmittance AccessoryThe Diffuse Transmittance Accessory (DTA)provides the capability to mount a variety ofsamples in front of an integrating spheredesigned for high performance and compliancewith regulatory standards. At only 2.9%, the port fraction complies with both AATCCmethod 183:2004 for measuring UltravioletProtection Factor (UPF) of fabrics and ASTMmethod D1003:2007 for measuring haze inplastics and glasses.
The optional fabric holder with includedUG11 filter makes fabric measurementseasy, and the magnetic steel baseplate withthreaded holes on a 1 inch grid supportsmagnetic or screw-down laser table fittingsto position almost any optical sample.
Diffuse Reflectance AccessoryThe Diffuse Reflectance accessory opens thedoor to measuring many samples that werealmost impossible to measure in the past.This accessory offers a horizontal mountingreflectance port to conveniently measureboth delicate and large samples. The doublebeam Spectralon® integrating sphere ensuresthe highest possible energy throughput andaccuracy. A transmittance port is included fordiffuse transmittance measurement of solidor turbid liquid materials. The integratingsphere is fully CIE compliant for performingcolor measurements.
Solid Sample Holder AccessoryThe Solid Sample Holder provides thecapacity to measure a variety of solid samples in % Transmission mode. Anassortment of sample holders is availablefor round, square and odd shaped samples.The 2"x 3" sample slides allow other measurement tools, such as specularreflectance accessories, to be used.
High-performance Measurements for Material Science
The Evolution 300/600 UV-Vis spectrophotometers offer a variety of accessories designed to meet your solid sample testing needs. With the horizontal mounting of our Diffuse Reflectance Accessory (DRA) or the highlyoptimized Diffuse Transmittance Accessory, a new world of materials sciencemeasurements is available with the Evolution spectrophotometers. Whateveryour routine high-performance measurements require, we have a variety ofsample handling accessories catering to your needs.
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The horizontal sample port of the DRA providesalmost unlimited sample size measurements.Using the DRA in combination with VISIONlite™
ColorCalc software provides routine solutionsfor CIE Color compliant measurements on solids.
The Diffuse Transmittance Accessorytogether with VISIONlite MaterialsCalcsoftware makes haze and fabric measurements simple and routine.
VISIONlite™ MaterialsCalc software provides an array of calculations ranging from Luminous Transmittance, Haze,
UV protection and UPF protection of fabrics. One of the keycalculations is the luminous transmittance of sunglasses. An example of the transmittance profile of a pair of high
end sunglasses is shown here along with the luminoustransmittance calculation.
The Evolution 300/600 UV-Vis series providesSmart Accessories with auto-intelligence. BothLocal Control and PC Control configurationsrecognize individual accessories and auto-matically identify the accessory’s uniqueserial number. This saves time and enhancesproductivity in regulated environments.Kinematic mounts allow easy interchangeand reproducible alignment each time theaccessory is removed and installed.
Smart Accessories intelligently interactwith software menus and local control.Simply snap the accessory in place and theappropriate software menu for that accessorywill appear on the screen. All other accessorysoftware menus are hidden eliminating possible confusion.
Evolution 300 and Evolution 600 U
V-Vis Spectrophotometers
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Smart Accessories for Enhanced Productivity
Smart Calibration Validation Carousel (CVC)Cylindrical Cell Holder
Diffuse Transmittance Accessory
Smart 8-Cell Peltier System
Adjustable Microcell Holder
Praying Mantis Accessory
Smart Thermostatted Rotary 7-Cell Changer Smart Peltier Thermostatted Single Cell Holder
Grazing Angle SpecularReflectance Accessory
VeeMAX Variable Angle 30° - 80°Specular Reflectance Accessory
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Smart Sipper Accessory
Test Tube and Vial Holder
VERSA Fiber Optic Probe
Long Pathlength Rectangular Cell Holder
8°, 15°, 20°, 30°, 45°, and 60° Specular Reflectance Accessories
Solid Sample HolderDRA-EV-600 Diffuse
Reflectance Accessory
Temperature Probe
RX2000 Rapid Mix Accessory
nanoCellAccessory
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consumables suited to your application.
To learn more about our products and comprehensive service offerings,
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