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For research use only. Not for use in diagnostic procedures.
Culture to ID in24 Minutes
As Low as$6.00 USD Per Test
Common GrowthMedia Used
No Upfront Tests orBiochemical Cards
Over 600 AerobesIdentified in One Test
Sherlock Q-FAMETM Sample Preparation Method
Q-FAME is the newest rapid identification method for the Sherlock®
Microbial Identification System (MIS), which has been optimized forclinical laboratories needing STAT processing of cultures. Q-FAMEtakes less than 25 minutes to identify aerobic bacteria from pureculture, often directly from the primary isolation plate.
The Sherlock® Microbial Identification System (MIS)
Over 750 clinical and environmental laboratories worldwide have usedthe Sherlock® MIS since 1991. The system identifies over 1,500bacterial and yeast species by analyzing the unique fatty acidfingerprints inherent within each species. Fatty acids are separatedfrom each microbe, converted to fatty acid methyl esters (FAMEs)and analyzed by Gas Chromatography (GC).
Sherlock automates all hardware operations and uses sophisticatedpattern recognition software to compare each unknown FAME profileto known FAME profiles for an identification. Additional software toolsallow for sample clustering, strain tracking, custom library generation,21CFR Part 11 compliance and DNA sequencing analyses.
Sherlock is fully automated with no GC experience required.
Sherlock Q-FAMETM
The New Standard for Rapid Bacterial Identification
Q-FAMETM Rapid Microbial ID Method
Benefits of Q-FAME vs. Other Aerobic Bacterial ID Systems
Rapid sample preparation and ID- The Q-FAME identification procedure takes less then 25 minutes to identify aerobic
bacteria from pure culture, at least 300% faster than other culture-based ID methods.
Reduced growing time required- The Q-FAME procedure often requires only 24 hours of growth from the primary isolation
plate, compared to 48 hours of growth required of other culture-based ID methods.-
Fewer bacterial cells required- The Q-FAME procedure uses ~7.0mg of cells, at least 40% fewer cells than other
culture-based ID methods.
Lower cost per sample- The Q-FAME procedure costs as low as $6.00 USD per sample, at least 60% lower cost
for consumables than other culture-based ID methods.
Standardized procedure for all aerobic bacteria- All isolates are prepared using the same procedure, so no upfront biochemical tests,
Gram stains or biochemical cards are required.
Benefits of Q-FAME vs. the Classical MIDI Extraction Procedure
Reduced turnaround time (TAT)- The TAT for one sample is reduced from 120 minutes to 24 minutes, thus making
Q-FAME more amenable to clinical STAT processing of samples.
Less corrosive reagents- Q-FAME reagents are less corrosive and MTBE, a ground water and soil contaminant, is
no longer a reagent used within the extraction procedure.
No more water baths or laboratory shakers- Q-FAME does not require any of these pieces of equipment.
Standard media and growth temperatures used- Clinical Library: Bacteria typically grown on TSA Agar with 5% defibrinated
sheep blood at 35C.- Environmental Library: Bacteria grown on TSA Agar at 30C.
Improved Entries with updated taxonomy- Q-FAME library entries were developed with up-to-date taxonomy.
Reduced wear on the GC Liner- The Q-FAME extract is cleaner and GC liners last twice as long as before.
Specifications
Q-FAME Start Up Kit Contents Aerobic Clinical Library (QBA1) – Bacteria typically grown at 35C on
TSA Agar with 5% defibrinated sheep blood. Aerobic Environmental Library (QTSA1) – Bacteria grown at 30C on TSA Agar 3 liquid dispensers 1 mini vortexer* 1 mini centrifuge 2 vial rack trays 1 reference pipette 1 dry block heater 1 aluminum heat block 1 right angle thermometer Glass conical inserts Clear 2ml GC vials PTFE/Silicon/PTFE caps 3 Q-FAME reagents shipped in Qorpack bottles Hexane wash reagent 1ul sterile inoculation loops Epi-tip pipette tips 1 MIDI GC injection port liner 1 box MIDI Rapid Calibration Standard 1 custom wooden stand
* Optional digital vortexer, foam insert & inset retainer available
Q-FAME Refill Kit Contents – 1 Size Available for 192 Samples Glass conical inserts Clear 2ml GC vials PTFE/Silicon/PTFE caps 3 Q-FAME extraction reagents shipped in Qorpack bottles Hexane wash reagent 1ul sterile inoculation loops Epi-tip pipette tips 1 MIDI GC injection port liner 1 box MIDI Rapid Calibration Standard
Customer Required Items Sherlock® Microbial Identification System version 6.0 or higher Agilent 6850, 6890 or 7890 model GCs, configured to MIDI specifications Clinical aerobe library – 35C Incubator & BBL Brand Trypticase Soy Agar with 5%
defibrinated sheep blood Environmental aerobe library – 30C Incubator & BBL Brand Trypticase Soy Agar Pasteur pipettes, 5 inch, disposable Chemical storage cabinets (for corrosives and flammables) Hazardous chemical waste disposal container Hazardous medical waste disposal container Chemical fume hood (depends on customer’s internal SOPs)
Sherlock Q-FAME Libraries
Genus-species QBA1 QTSA1
Achromobacter-xylosoxidans
Acidovorax-avenae
Acidovorax-delafieldii
Acidovorax-facilis
Acinetobacter-baumannii
Acinetobacter-calcoaceticus
Acinetobacter-haemolyticus
Acinetobacter-johnsonii
Acinetobacter-lwoffii
Acinetobacter-radioresistens
Actinobacillus-lignieresii
Actinobacillus-ureae
Aeromonas-hydrophila
Aeromonas-salmonicida
Aeromonas-schubertii
Aeromonas-sobria
Aeromonas-trota
Aeromonas-veronii
Aggregatibacter-aphrophilus
Aggregatibacter-segnis
Alcaligenes-faecalis
Amycolatopsis-orientalis
Aneurinibacillus-aneurinilyticus
Arcanobacterium-haemolyticum
Arcanobacterium-pyogenes
Arthrobacter-aurescens
Arthrobacter-crystallopoietes
Arthrobacter-globiformis
Arthrobacter-pascens
Avibacterium-paragallinarum
Bacillus-amyloliquefaciens
Bacillus-atrophaeus
Bacillus-badius
Bacillus-cereus
Bacillus-circulans
Bacillus-coagulans
Bacillus-firmus
Bacillus-freudenreichii"
Bacillus-gibsonii
Bacillus-lentus
Bacillus-licheniformis
Bacillus-megaterium
Genus-species QBA1 QTSA1
Bacillus-mycoides
Bacillus-pumilus
Bacillus-simplex
Bacillus-subtilis
Bacillus-thuringiensis
Bartonella-henselae
Bibersteinia-trehalosi
Bordetella-avium
Bordetella-bronchiseptica
Bordetella-holmesii
Bordetella-parapertussis
Bordetella-pertussis
Bordetella-trematum
Brevibacillus-agri
Brevibacillus-borstelensis
Brevibacillus-brevis
Brevibacillus-choshinensis
Brevibacillus-laterosporus
Brevibacillus-parabrevis
Brevibacterium-casei
Brevibacterium-epidermidis
Brevibacterium-iodinum
Brevibacterium-linens
Brevundimonas-diminuta
Brevundimonas-vesicularis
Burkholderia-anthina
Burkholderia-caryophylli
Burkholderia-cenocepacia
Burkholderia-cepacia
Burkholderia-cepacia complex
Burkholderia-gladioli
Burkholderia-multivorans
Burkholderia-pyrrocinia
Campylobacter-coli
Campylobacter-fetus
Campylobacter-jejuni
Cardiobacterium-hominis
Cedecea-davisae
Cedecea-lapagei
Cellulomonas-fimi
Cellulosimicrobium-cellulans
Chromobacterium-violaceum
Sherlock Q-FAME Libraries
Genus-species QBA1 QTSA1
Chryseobacterium-balustinum
Chryseobacterium-gleum
Chryseobacterium-indologenes
Chryseomonas-luteola
Citrobacter-farmeri
Citrobacter-freundii
Citrobacter-koseri
Clavibacter-michiganensis
Comamonas-terrigena
Comamonas-testosteroni
Corynebacterium-afermentans
Corynebacterium-amycolatum
Corynebacterium-auris
Corynebacterium-bovis
Corynebacterium-coyleae
Corynebacterium-diphtheriae
Corynebacterium-flavescens
Corynebacterium-jeikeium
Corynebacterium-kutscheri
Corynebacterium-minutissimum
Corynebacterium-pseudodiphtheriticum
Corynebacterium-pseudotuberculosis
Corynebacterium-renale
Corynebacterium-striatum
Corynebacterium-urealyticum
Corynebacterium-xerosis
Cronobacter-sakazakii
Curtobacterium-flaccumfaciens
Curtobacterium-luteum
Curtobacterium-pusillum
Deinococcus-radiodurans
Delftia-acidovorans
Dermabacter-hominis
Dietzia-maris
Edwardsiella-hoshinae
Edwardsiella-tarda
Eikenella-corrodens
Elizabethkingia-meningoseptica
Empedobacter-brevis
Enterobacter-aerogenes
Enterobacter-amnigenus
Genus-species QBA1 QTSA1
Enterobacter-asburiae
Enterobacter-cancerogenus
Enterobacter-cloacae
Enterobacter-gergoviae
Enterobacter-hormaechei
Enterococcus-avium
Enterococcus-casseliflavus
Enterococcus-cecorum
Enterococcus-columbae
Enterococcus-dispar
Enterococcus-durans
Enterococcus-faecalis
Enterococcus-faecium
Enterococcus-gallinarum
Enterococcus-hirae
Enterococcus-malodoratus
Enterococcus-mundtii
Enterococcus-raffinosus
Escherichia-coli
Escherichia-fergusonii
Exiguobacterium-acetylicum
Flavimonas-oryzihabitans
Flavobacterium-johnsoniae
Flavobacterium-mizutaii
Fluoribacter-bozemanae
Fluoribacter-dumoffii
Fluoribacter-gormanii
Francisella-philomiragia
Gardnerella-vaginalis
Geobacillus-stearothermophilus
Geobacillus-thermoglucosidasius
Gordonia-aichiensis
Gordonia-amarae
Gordonia-bronchialis
Gordonia-rubripertincta
Gordonia-sputi
Gordonia-terrae
Haemophilus-influenzae
Haemophilus-parahaemolyticus
Haemophilus-parainfluenzae
Hafnia-alvei
Helicobacter-pylori
Sherlock Q-FAME Libraries
Genus-species QBA1 QTSA1
Hydrogenophaga-pseudoflava
Jonesia-denitrificans
Kingella-denitrificans
Kingella-kingae
Klebsiella-oxytoca
Klebsiella-pneumoniae
Kluyvera-ascorbata
Kocuria-kristinae
Kocuria-rhizophila
Kocuria-rosea
Kocuria-varians
Kytococcus-sedentarius
Lactobacillus-delbrueckii
Lactobacillus-pentosus
Lactobacillus-plantarum
Lactobacillus-reuteri
Lactobacillus-rhamnosus
Lactococcus-garvieae
Lactococcus-lactis
Lactococcus-plantarum
Leclercia-adecarboxylata
Legionella-geestiana
Legionella-jordanis
Legionella-pneumophila
Legionella-sainthelensi
Leminorella-grimontii
Leuconostoc-mesenteroides
Leuconostoc-pseudomesenteroides
Listeria-grayi
Listeria-innocua
Listeria-ivanovii
Listeria-monocytogenes
Listeria-seeligeri
Lysinibacillus-sphaericus
Macrococcus-caseolyticus
Methylobacterium-aminovorans
Methylobacterium-extorquens
Methylobacterium-fujisawaense
Methylobacterium-mesophilicum
Methylobacterium-organophilum
Methylobacterium-radiotolerans
Methylobacterium-rhodesianum
Genus-species QBA1 QTSA1
Methylobacterium-zatmanii
Microbacterium-aurantiacum
Microbacterium-aurum
Microbacterium-barkeri
Microbacterium-chocolatum
Microbacterium-dextranolyticum
Microbacterium-esteraromaticum
Microbacterium-flavescens
Microbacterium-hominis
Microbacterium-hydrocarbonoxydans
Microbacterium-imperiale
Microbacterium-keratanolyticum
Microbacterium-lacticum
Microbacterium-laevaniformans
Microbacterium-liquefaciens
Microbacterium-luteolum
Microbacterium-maritypicum
Microbacterium-saperdae
Microbacterium-schleiferi
Microbacterium-terrae
Microbacterium-testaceum
Micrococcus-luteus
Micrococcus-lylae
MIDI-Calibration
Moellerella-wisconsensis
Moraxella-atlantae
Moraxella-bovis
Moraxella-canis
Moraxella-catarrhalis
Moraxella-nonliquefaciens
Moraxella-osloensis
Morganella-morganii
Mycobacterium-abscessus
Mycobacterium-agri
Mycobacterium-aurum
Mycobacterium-brumae
Mycobacterium-chelonae
Mycobacterium-chitae
Mycobacterium-chlorophenolicum
Mycobacterium-chubuense
Mycobacterium-diernhoferi
Mycobacterium-fallax
Sherlock Q-FAME Libraries
Genus-species QBA1 QTSA1
Mycobacterium-flavescens
Mycobacterium-fortuitum
Mycobacterium-komossense
Mycobacterium-moriokaense
Mycobacterium-mucogenicum
Mycobacterium-neoaurum
Mycobacterium-obuense
Mycobacterium-smegmatis
Mycobacterium-sphagni
Mycobacterium-vaccae
Myroides-odoratus
Neisseria-cinerea
Neisseria-elongata
Neisseria-flava
Neisseria-flavescens
Neisseria-gonorrhoeae
Neisseria-meningitidis
Neisseria-mucosa
Neisseria-sicca
Neisseria-subflava
Neisseria-weaveri
Nocardia-asteroides
Nocardia-brasiliensis
Nocardia-brevicatena
Nocardia-farcinica
Nocardia-globerula
Nocardia-nova
Nocardia-otitidiscaviarum
Nocardia-pseudobrasiliensis
Nocardioides-albus
Novosphingobium-aromaticivorans
Novosphingobium-capsulatum
Novosphingobium-subterraneum
Ochrobactrum-anthropi
Oerskovia-turbata
Oligella-ureolytica
Oligella-urethralis
Paenibacillus-alvei
Paenibacillus-amylolyticus
Paenibacillus-azotofixans
Paenibacillus-glucanolyticus
Paenibacillus-lautus
Genus-species QBA1 QTSA1
Paenibacillus-macerans
Paenibacillus-pabuli
Paenibacillus-peoriae
Paenibacillus-polymyxa
Paenibacillus-thiaminolyticus
Paenibacillus-validus
Pandoraea-apista
Pantoea-agglomerans
Paracoccus-denitrificans
Pasteurella-multocida
Pectobacterium-carotovorum
Plesiomonas-shigelloides
Proteus-mirabilis
Proteus-penneri
Proteus-vulgaris
Providencia-alcalifaciens
Providencia-rettgeri
Providencia-rustigianii
Pseudomonas-aeruginosa
Pseudomonas-alcaligenes
Pseudomonas-chlororaphis
Pseudomonas-fluorescens
Pseudomonas-luteola
Pseudomonas-mendocina
Pseudomonas-pseudoalcaligenes
Pseudomonas-putida
Pseudomonas-savastanoi
Pseudomonas-stutzeri
Pseudomonas-syringae
Psychrobacter-phenylpyruvicus
Ralstonia-eutropha
Ralstonia-pickettii
Ralstonia-solanacearum
Rhizobium-radiobacter
Rhodococcus-coprophilus
Rhodococcus-equi
Rhodococcus-erythropolis
Rhodococcus-rhodochrous
Riemerella-anatipestifer
Roseomonas-cervicalis
Roseomonas-fauriae
Roseomonas-gilardii
Sherlock Q-FAME Libraries
Genus-species QBA1 QTSA1
Salmonella-enterica
Serratia-liquefaciens
Serratia-marcescens
Serratia-odorifera
Serratia-rubidaea
Shewanella-putrefaciens
Shigella-boydii
Shigella-dysenteriae
Shigella-flexneri
Shigella-sonnei
Sphingobacterium-multivorum
Sphingobacterium-spiritivorum
Sphingomonas-adhaesiva
Sphingomonas-macrogoltabidus
Sphingomonas-parapaucimobilis
Sphingomonas-paucimobilis
Sphingomonas-terrae
Sphingomonas-yanoikuyae
Staphylococcus-arlettae
Staphylococcus-aureus
Staphylococcus-auricularis
Staphylococcus-capitis
Staphylococcus-caprae
Staphylococcus-carnosus
Staphylococcus-chromogenes
Staphylococcus-cohnii
Staphylococcus-delphini
Staphylococcus-epidermidis
Staphylococcus-equorum
Staphylococcus-felis
Staphylococcus-gallinarum
Staphylococcus-haemolyticus
Staphylococcus-hominis
Staphylococcus-hyicus
Staphylococcus-intermedius
Staphylococcus-kloosii
Staphylococcus-lentus
Staphylococcus-lugdunensis
Staphylococcus-lutrae
Staphylococcus-muscae
Staphylococcus-pasteuri
Staphylococcus-saprophyticus
Genus-species QBA1 QTSA1
Staphylococcus-schleiferi
Staphylococcus-sciuri
Staphylococcus-simulans
Staphylococcus-warneri
Staphylococcus-xylosus
Stenotrophomonas-maltophilia
Streptococcus-agalactiae
Streptococcus-bovis
Streptococcus-canis
Streptococcus-cristatus
Streptococcus-dysgalactiae
Streptococcus-equi
Streptococcus-equinus
Streptococcus-gallolyticus
Streptococcus-gordonii
Streptococcus-infantarius
Streptococcus-infantis
Streptococcus-iniae
Streptococcus-intermedius
Streptococcus-mitis
Streptococcus-mutans
Streptococcus-oralis
Streptococcus-parasanguinis
Streptococcus-pneumoniae
Streptococcus-pyogenes
Streptococcus-salivarius
Streptococcus-sanguinis
Streptococcus-suis
Suttonella-indologenes
Tatlockia-maceachernii
Tatlockia-micdadei
Tsukamurella-paurometabola
Vibrio-alginolyticus
Vibrio-cholerae
Vibrio-mimicus
Vibrio-parahaemolyticus
Vibrio-vulnificus
Virgibacillus-pantothenticus
Weeksella-virosa
Weissella-confusa
Xanthobacter-agilis
Xanthobacter-flavus
Sherlock Q-FAME Libraries
Genus-species QBA1 QTSA1
Xanthomonas-axonopodis
Xanthomonas-campestris
Xanthomonas-hortorum
Yersinia-enterocolitica
Yersinia-frederiksenii
Yersinia-intermedia
Yersinia-kristensenii
Yersinia-pseudotuberculosis
Yersinia-ruckeri
The information in thispublication is subject tochange without notice.
Copyright 2010 MIDI, Inc.All Rights Reserved
Date: 01 September 2010
MIDI, Inc.125 Sandy DriveNewark, Delaware 19713USAPhone: 302-737-4297Fax: 302-737-7781Email: [email protected]
www.midi-inc.com
