Embed Size (px)
Citation preview
F eat u r es
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs
The Maxwell® 16 SystemFast. Simple. Reliable.
• purify samples in 30–45 minutes while you focus on other lab activities.
• purify dnA, rnA and recombinant protein from 1–16 samples.
• Automation improves consistency by eliminating manual processing.
• predispensed reagent cartridges save time and improve yield consistency.
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
au to M at i o N
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs
An automated system
1. the sample is mixed by rapid up-and-down movement of the single-use plunger.
2. the paramagnetic particles are captured after the plunger is magnetized.
3. the paramagnetic particles bind gdnA.
4. gdnA is purified through a series of capture and release washes.
5. the purified gdnA is released into the elution tube.
2 3 4 5 6 7
PredispensedMaxwell® 16 Blood
DNA Cartridge
50–400µlof human
blood
7127
MB
2 3 4 5 6 7
1 3 4 5 6 7
1 2 4 5 6
6
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
t wo Fo r M ats
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs68
63TA
8460
TA
Maxwell® 16 is one instrument with two formats
standard Elution Volume (sEV)300 µl
low Elution Volume (lEV)50 µl
9273
MA
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
s e V B lo o d k i t
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs
Maxwell® 16 Blood DNA Purification Kit (SEV)Two protocols, same reagent kit
• 50–400µl of whole blood or
• 250–500µl of buffy coat cells (2.5–5.0ml of whole blood)
Add anti-coagulated whole blood or buffy coat directly to reagent cartridge.
Maxwell® 16 Blood DNA Purification Kit offers:
• Fast extractions using reliable paramagnetic particles
• Hands-free processing of up to 16 samples/run
• Consistent gdnA yields and purity from operator-to-operator
• walk-away automation to accomplish more in the lab
9271
MA
maxwell® 16 Blood dnA purification Cartridge
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
reliaBle results
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs
Maxwell® 16 Blood DNA Purification Kit (SEV)Consistent gDNA yields from fresh blood, frozen blood, or buffy coat (>3pg/wbc)
Input volume of fresh whole blood (µl)
Storage conditions of whole blood
400 300 200 100 75 50
+4˚C -20˚C rt
Apoptotic dnA
Buffy coat gdnA >100ng/µl
5652TA
M 4°C –20°C Room Temp.{
Yield comparison of whole blood vs buffy coat
data shown above illustrates gdnA run by agarose gel electrophoresis.
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
coNsisteNt yields
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs
Maxwell® 16 Blood DNA Purification Kit (SEV)Consistent gDNA yields for PCR amplification
5650
TA
A.
B.
23kbM 1 2 3 4 5 6
M B1 2 3 4 5 6
5650
TA
A.
B.
23kbM 1 2 3 4 5 6
M B1 2 3 4 5 6
dnA isolated from blood samples obtained from six donors.
Panel A. Aliquots from each sample were resolved by agarose gel electrophoresis (lane m: lambda dnA/Hindiii markers). Panel B. Equal volumes of each sample were amplified using a primer pair specific for Human growth Factor ii gene (lane m: dnA ladder; lane B: no-template control).
typical gdnA yields from Blood
s a M p l e t y p e s a M p l e s i z e g d N a y i e l d *
whole blood 200µl 4–9µg
whole blood 400µl 8–15µg
* 3μg/ml white blood cells
A B
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
8460
TA
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
l e V B lo o d k i t
simplE prEproCEssing
CompAtiBility
rEsults
summAry
rEsourCEs
Maxwell® 16 LEV Blood DNA KitPurification technology developed for high-efficiency isolation
nEw MagnaCel™ paramagnetic particle
• High binding capacity (20 ug dnA/2.5 mg paricles)
• large sample size processing (400 µl-500µl)
• Highly concentrated gdnA (up to 200ng/µl) with lEV format
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
siMple preprocessiNg
CompAtiBility
rEsults
summAry
rEsourCEs
Maxwell® 16 LEV Blood DNA KitOptimized for maximum yield and purity
Simple three-step preprocessing
• reagents + Blood
• incubate
• run
get >160ng/µl purified dnA
Whole Blood 300–400µl and Proteinase K
Incubate 15 minutes at 56˚C
Load Cartridge
Vortex lysate
Run for 40 Minutes
Add Lysis Buffer
9272
MA
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
coMpatiBility
rEsults
summAry
rEsourCEs
Maxwell® 16 LEV Blood DNA KitCompatible with all commonly used collection tubes
0 50
100 150 200 250 300
EDTA
(n=4
) So
dium
Citr
ate
(n=4
) So
dium
Hep
arin
(n=4
) ED
TA (n
=4)
Sodi
um C
itrat
e (n
=4)
Sodi
um H
epar
in (n
=4)
1 2
Con
cent
ratio
n (n
g/l)
Sample
9274
MA
0 0.2 0.4 0.6 0.8
1 1.2 1.4 1.6 1.8
2
EDTA
(n=4
) So
dium
Citr
ate
(n=4
) So
dium
Hep
arin
(n=4
) ED
TA (n
=4)
Sodi
um C
itrat
e (n
=4)
Sodi
um H
epar
in (n
=4)
1 2 Sample
A260:A280
A260:A230
9275
MA
whole blood in various collection tubes.
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
results
summAry
rEsourCEs
Maxwell® 16 LEV Blood DNA KitExceptional gDNA recovery up to 400µl
data demonstrates a linear increase in extracted dnA between 50-400µl
% yield remains high at all volumes
y = 0.4932x R = 0.97632
0.00
50.00
100.00
150.00
200.00
250.00
0 100 200 300 400 500
ng/
l
Blood volume ( l)
9283MA
0.00
20.00
40.00
60.00
80.00
100.00
120.00
400 (n=4) 200 (n=4) 100 (n=4) 50 (n=4)
% Y
ield
Blood volume ( l)
9284MA
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
s u M M a ry
rEsourCEs
Maxwell® 16 Blood DNA Purification KitsReliable convenience of automated gDNA purification
• Simple protocol: maxwell® 16 system is programmed for automating the maxwell® Blood dnA purification Kits for easy gdnA recovery from blood.
• Fast purification: rapid isolation of gdnA from blood within 45 minutes on the maxwell® 16 instrument.
• Reliable: Consistently pure gdnA and high yields from operator-to-operator and from day-to-day.
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t
FEAturEs
AutomAtion
two FormAts
sEV Blood Kit
rEliABlE rEsults
ConsistEnt yiElds
lEV Blood Kit
simplE prEproCEssing
CompAtiBility
rEsults
summAry
r es o u rc es
ResourcesTechnical Manuals: As1010, As1290, As2000
Applications: service & support
References: pubmed citations list
maxwell is a registered trademark of promega Corporation. magnaCel is a trademark of promega Corporation.
promEgA CorporAtion • 2800 woods Hollow roAd • mAdison, wi 53711-5399 usA • tElEpHonE 608-274-4330 www.promega.com • ©2010 promEgA CorporAtion • All rigHts rEsErVEd • priCEs And spECiFiCAtions suBjECt to CHAngE witHout prior notiCE • printEd in usA 8/10 • 18910 • pArt #is001
Ordering informationp r o d u c t c ata l o g #
M a x w e l l ® 16 B l o o d d N a p u r i F i c at i o N k i t * a s 1010
M a x w e l l ® 16 l e V B l o o d d N a k i t a s 1290
M a x w e l l ® 16 i N s t r u M e N t a s 2000
* u.s. pat. nos. 6,027,945 and 6,368,800, Australian pat. no. 732756 and other patents pending.
