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The MACSQuant® Analyzer 16Colorful new possibilities in automated flow cytometry
Germany/Austria/ SwitzerlandMiltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 [email protected]
USA/CanadaMiltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 877 591 1060 [email protected]
AustraliaMiltenyi Biotec Australia Pty. Ltd. Unit 16 A, 2 Eden Park Drive Macquarie Park NSW 2113 Australia Phone +61 2 8877 7400 Fax +61 2 9889 5044 [email protected]
BeneluxMiltenyi Biotec B.V. Sandifortdreef 17 2333 ZZ Leiden The Netherlands [email protected] service The NetherlandsPhone 0800 4020120 Fax 0800 4020100Customer service BelgiumPhone 0800 94016 Fax 0800 99626Customer service LuxembourgPhone 800 24971 Fax 800 24984
ChinaMiltenyi Biotec Technology & Trading (Shanghai) Co., Ltd. Rooms 2303 and 2309 No. 319, Xianxia Road Changning District 200051 Shanghai, P.R. China Phone +86 21 62351005 Fax +86 21 62350953 [email protected]
FranceMiltenyi Biotec SAS 10 rue Mercoeur 75011 Paris, France Phone +33 1 56 98 16 16 Fax +33 1 56 98 16 17 [email protected]
ItalyMiltenyi Biotec S.r.l. Via Paolo Nanni Costa, 3040133 Bologna Italy Phone +39 051 6 460 411 Fax +39 051 6 460 499 [email protected]
JapanMiltenyi Biotec K.K. Nittsu-Eitai Building 5F 16-10 Fuyuki, Koto-ku, Tokyo 135-0041, Japan Phone +81 3 5646 8910 Fax +81 3 5646 8911 [email protected]
Nordics and BalticsMiltenyi Biotec Norden AB Scheelevägen 17 223 70 Lund Sweden [email protected] service SwedenPhone 0200-111 800 Fax 046-280 72 99 Customer service DenmarkPhone 80 20 30 10 Fax +46 46 280 72 99 Customer service Norway, Finland, Iceland, and Baltic countriesPhone +46 46 280 72 80 Fax +46 46 280 72 99
SingaporeMiltenyi Biotec Asia Pacific Pte Ltd. 100 Beach Road #28-06 to 28-08 Shaw Tower Singapore 189702 Phone +65 6238 8183 Fax +65 6238 0302 [email protected]
South KoreaMiltenyi Biotec Korea Co., Ltd Arigi Bldg. 8F 562 Nonhyeon-ro Gangnam-gu Seoul 06136, South Korea Phone +82 2 555 1988 Fax +82 2 555 8890 [email protected]
SpainMiltenyi Biotec S.L. C/Luis Buñuel 2 Ciudad de la Imagen 28223 Pozuelo de Alarcón (Madrid) Spain Phone +34 91 512 12 90 Fax +34 91 512 12 91 [email protected]
United KingdomMiltenyi Biotec Ltd. Almac House, Church Lane Bisley, Surrey GU24 9DR, UK Phone +44 1483 799 800 Fax +44 1483 799 811 [email protected]
www.miltenyibiotec.com
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Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Copyright owl image: © Miles Away Photography/Shutterstock.com
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, MACSQuant, REAfinity, and Vio are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its affiliates in various countries worldwide. Copyright © 2018 Miltenyi Biotec GmbH and/or its affiliates. All rights reserved.
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The MACSQuant® Analyzer 16 is engineered to expand the revolutionary automation of the MACSQuant Analyzer line of flow cytometers. It facilitates fully automated data acquisition in a 24-tube or 96-well plate format. The instrument’s compact design is suited for basic research as well as advanced immune monitoring applications. The MACSQuant Analyzer 16 provides users with the flexibility and customization that are required for the increasing demands of modern laboratories.
AutomationLoad your samples, import your experiment settings, and leave the staining, data acquisition and analysis to the MACSQuant Analyzer 16.
Expanded fluorescent channelsAmplify your data with a selection from 14 fluorescence channels and an average of 40% less sample volume required.
FlexibilityEasily and immediately switch between tubes and plates at the click of a button.
SimplicityMinimize the learning curve with straight forward experiment setup and operation.
Multi-instrument alignmentUsing our Smart Gain software technology, users can harmonize data with collaborating labs to ensure reproducibility.
More colors, more applications
ColorfulAutomationSimplicity
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Renowned instrumentation with expanded fluorescence capabilities
As research questions become more complex, let the MACSQuant® Analyzer 16 simplify your data acquisition. With expanded fluorescent capabilities, you can now maximize the number of parameters you can measure for each sample. Fluorescent channels are available with 405, 488 and 640 nm excitation lasers to cover a broad range of dyes and fluorescent
proteins, enabling you to tell your story with more color. With the MACSQuant Analyzer 16, you can expand your immunophenotyping applications to span a broad range of cell types, such as T cells, B cells, NK cells, and dendritic cells, as well as tissue-specific, neural and stem cell populations. What would new possibilities mean for your research?
FSC 488/10 nm
B1 525/50 nm FITC, GFP
B2 579/34 nm PE
B3 615/20 nm PE-Vio® 615
B4 667/30 nm PerCP
B5 725/40 nm PerCP-Vio 700
B6 785/62 nm PE-Vio770
Blue laser 488 nm
R1 667/30 nm APC
R2 725/40 nm AF700
R3 785/62 nm APC-Vio 770
Red laser 640 nm
SSC 405/10 nm
V1 450/50 nm VioBlue®
V2 525/50 nm VioGreen™
V3 579/34 nm BV 570
V4 615/20 nm BV 605
V5 667/30 nm BV 650
Violet laser 405 nm
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The features that make the difference
Universal Reagent Rack: Flexible autolabeling options from glass or plastic vials
Robotic needle arm: At the heart of sample mixing, processing and autolabeling
MiniSampler: Seamless sampling from single tubes, multiple tubes or 96-well plate
Large monitor: 15.6" monitor for simple touchscreen operation
Integrated MACS® Magnetic Cell Enrichment:Focus on your cells of interest and fortify your data
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With the inclusion of the MACS® MiniSampler Plus, the MACSQuant® Analyzer 16 enables autolabeling of your samples using the automated pipetting arm. The newly designed Universal Reagent Rack allows you to add reagents to your tubes or plates from 5 mL glass or 1 mL plastic vials.
Why risk the variability when you can automate your applicationsReduce the risk of pipetting error with the reliability of robotics. All you have to do is program the titer of your reagent, the time for incubation and the dilution of your sample, if applicable. That’s it! You are now ready for the instrument to label and prepare your samples automatically.
Autolabeling The missing step in a fully automated flow cytometry assay
Focus on the cells you desireUsing the integrated MACS® Enrichment Column, you can magnetically enrich your target population to perform a deeper analysis of rare cell subsets. Removing the non-relevant events before your flow analysis make your assay even more robust.
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Get more with the MACSQuant Analyzer 16More channels. More data. More subsets.
• Conserve sample – Up to 40% less material
• Conserve reagents – Up to 40% less reagent
• Conserve your time – 24-tube and 96-well plate automated acquisition
Multiparameter analysis of PBMC subpopulations using Flowlogic™SoftwareAmplifying your data has never been so easy. An example is shown here with a gating strategy for a 12-color panel designed to analyze T cell, B cell, NK cell, dendritic cell, and myeloid cell subsets from one sample of peripheral mononuclear cells (PBMCs). The data was analyzed using Flowlogic™ Software, which comes with many automated gating, graphing, and reporting features and generates ready-to-use, high-quality data.
Dive
deeper
into
your
subsets
Singlets
Scatter
T cells
NK cells Monocytes
B cells
CD8+ T cells
CD4+ T cells
Neutrophils
cDC1
cDC2
Dendritic cells
Nano particle analysis with violet side scatterUtilize the violet side scatter optics of the MACSQuant Analyzer 16 for small particle analysis
160 nm
100 nm
Noise
240 nm
900 nm
200 nm
500 nm
300 nm
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Harmonizing the world is just an instrument setting away
Smart Gain with MACSQuantify™ SoftwareExperience consistently reproducible results from day to day, instrument to instrument, and operator to operator. With the Smart Gain technology of MACSQuantify Software, you can transfer your assay from one instrument to another, while passing on all necessary information to ensure an identical setup. As a result, the MACSQuant® Operating System produces comparable, standardized results, every time, everwhere.
Compliance with 21 CFR Part 11You can be sure that your data is suitable for submission to regulatory agencies with the optional 21 CFR Part 11 feature, which includes:
• Audit trail
• Analysis reports with e-singature
• User management system according to 21 CFR Part 11
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Your partner in cell therapyFurther characterize and enumerate your cells in your cell manufacturing applications. The MACSQuant® Analyzer 16 hardware and software are configured to support small to large scale manufacturing requirements.
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Complete your automation loop with reproducible reagents
Achieving maximum reproducibility between experiments cannot depend on the flow cytometer alone. In order to achieve consistent results, Miltenyi Biotec offers a complete flow cytometry solution including a dedicated range of reagents. We help you make sure that variations in your experiment are due to your sample and not due to unreliable antibodies or instruments.
REAfinity™ Recombinant Antibodies – flow cytometry is in their genesMiltenyi Biotec has introduced a portfolio of REAfinity™ Recombinant Antibodies that provide superior lot-to-lot consistency and purity compared to mouse or rat monoclonal and polyclonal antibodies. Our recombinant technology also diminishes the need for FcR blocking and allows for analyses with only one single isotype control, generating high quality data with no more background signal while saving efforts when setting up experiments. For more information, visit: www.miltenyibiotec.com/reafinity
Advantages of REAfinity Recombinant Antibodies:
• High lot-to-lot consistency
• One universal isotype
• No more background signal
Vio® Dyes – brighter dyes for flow cytometryWhen used in combination with our proprietary Vio® and VioBright™ Dyes, you can take advantage of superior mean fluorescence intensity and high stain indices. As the brightest dyes on the market, setting up complex multicolor experiments has never been so simple. For more information, visit: www.miltenyibiotec.com/vio
Ready-to-use kitsUse Miltenyi Biotec’s range of ready-to-use, pre-titrated kits and save valuable experiment set-up time and assay costs. Our kits have been validated to be used with the automatic labeling capacities of the MACSQuant® Analyzer 16, which in combination with our MACSQuant Express Modes, give you true walk-away capacities. All you have to do is set-up the experiment and come back to look at completely analyzed data.
Customized solutionsMiltenyi Biotec’s custom antibody design service enables researchers to benefit from personalized flow cytometry solutions. This service includes purified, functional-grade antibodies, single- and multicolor antibody conjugates as well as multicolor antibody cocktails. To find out more, visit: www.miltenyibiotec.com/customab
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MACSQuant® Analyzer 16 specifications
Optics
Laser excitation Spatially separated:405 nm, 65 mW diode488 nm, 50 mW DPSS (diode pumped solid state)640 nm, 72 mW diode
Emission detectors FSC: 488/10 nm SSC: 405/10 nm
B1: 525/50 nm B2: 579/34 nm B3: 615/20 nm B4: 667/30 nm B5: 725/40 nm B6: 785/62 nm
V1: 450/50 nm V2: 525/50 nm V3: 579/34 nm V4: 615/20 nm V5: 667/30 nm
R1: 667/30 nm R2: 725/40 nm R3: 785/62 nm
Fluorescence sensitivity (MESF) FITC < 110 PE < 75 APC < 100
Fluorescence precision (CV) < 5% CV with alignment verification particles
Scatter resolution Scatter performance is optimized for resolving human peripheral blood lymphocytes, monocytes, and granulocytes
Flow cell dimensions 200×250 µm
Fluorescence detectors Optimized with spectrally matched PMTs for all channels
Optical alignment Fixed tree-like configuration, no user adjustments needed
Laser spot size 15×45 µm
Fluidics
Minimal masurement volume 1 µL (25 µL for full spectrum)
Dead volume 10 µL
Sample flow rate 25–100 µL/minute plus automated flow rate to maintain 500, 1,000, or 2,000 events/second
Measurement speed 1,4 25 minutes per 96-well plate (5 µL measurement volume; screen mode)
Sample uptake Via robotic needle arm
Maximal event rate 15,000 events/second
System maintenance Automated startup, PMT calibration, cleaning cycles, and shutdown
Sample mixing Aspiration
Performance
Absolute counts performance (reproducibility) 1,2
CV < 7%
Sample carryover 1,3 0.01% (extended washing)
Sample tube/plates allowed 96-well plate (U, V, flat, deep well) FACS tubes (5 mL) Eppendorf tubes
MACS® Cell Enrichment Unit For pre-analysis enrichment of rare cells
1 Referred value indicates the average of multiple experiments and can differ for individual sample values.
2 For counting performance, full 96-well plates were loaded with 200 µL/well of peripheral blood mononuclear cell (PBMC) suspension at a nominal concentration of 5000 cells/µL. The uptake volume was set to 50 µL at medium flowrate.
3 For carry-over, full 96-well plates were loaded with 200 µL/well of PBMC suspension at a nominal concentration of 5000 cells/µL in every other well (“SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer (“CO-wells”). The uptake volume was set to 50 µL at medium flowrate. The carry-over is defined by sum(CO-singlet count)/sum(SRC-singlet count) ×100%.
4 The measurement speed is determined by measuring the time between the first movement of the robotic arm into the first measured well and the first movement out of the last well. The measurements itself were carried out at the highest possible flow rate in fast mode measuring 5 µL per well.
Service
• Comprehensive service options
• Globally distributed field service teams
Instrument training
• Training at regional MACS® Academy Miltenyi Biotec facilities
• Onsite training and assay development
• Online application resources
Application and instrument support
• Technical and field application support for assay design and product advice
• Custom automation and express mode development
MACSQuant® Live Support
• Live support at your fingertips via MACSQuant Support portal
• Have your questions answered in real-time by one of our experts
Support at your
fingertips
miltenyibiotec.com/mq16
Germany/Austria/ SwitzerlandMiltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 [email protected]
USA/CanadaMiltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 877 591 1060 [email protected]
AustraliaMiltenyi Biotec Australia Pty. Ltd. Unit 16 A, 2 Eden Park Drive Macquarie Park NSW 2113 Australia Phone +61 2 8877 7400 Fax +61 2 9889 5044 [email protected]
BeneluxMiltenyi Biotec B.V. Sandifortdreef 17 2333 ZZ Leiden The Netherlands [email protected] service The NetherlandsPhone 0800 4020120 Fax 0800 4020100Customer service BelgiumPhone 0800 94016 Fax 0800 99626Customer service LuxembourgPhone 800 24971 Fax 800 24984
ChinaMiltenyi Biotec Technology & Trading (Shanghai) Co., Ltd. Rooms 2303 and 2309 No. 319, Xianxia Road Changning District 200051 Shanghai, P.R. China Phone +86 21 62351005 Fax +86 21 62350953 [email protected]
FranceMiltenyi Biotec SAS 10 rue Mercoeur 75011 Paris, France Phone +33 1 56 98 16 16 Fax +33 1 56 98 16 17 [email protected]
ItalyMiltenyi Biotec S.r.l. Via Paolo Nanni Costa, 3040133 Bologna Italy Phone +39 051 6 460 411 Fax +39 051 6 460 499 [email protected]
JapanMiltenyi Biotec K.K. Nittsu-Eitai Building 5F 16-10 Fuyuki, Koto-ku, Tokyo 135-0041, Japan Phone +81 3 5646 8910 Fax +81 3 5646 8911 [email protected]
Nordics and BalticsMiltenyi Biotec Norden AB Scheelevägen 17 223 70 Lund Sweden [email protected] service SwedenPhone 0200-111 800 Fax 046-280 72 99 Customer service DenmarkPhone 80 20 30 10 Fax +46 46 280 72 99 Customer service Norway, Finland, Iceland, and Baltic countriesPhone +46 46 280 72 80 Fax +46 46 280 72 99
SingaporeMiltenyi Biotec Asia Pacific Pte Ltd. 100 Beach Road #28-06 to 28-08 Shaw Tower Singapore 189702 Phone +65 6238 8183 Fax +65 6238 0302 [email protected]
South KoreaMiltenyi Biotec Korea Co., Ltd Arigi Bldg. 8F 562 Nonhyeon-ro Gangnam-gu Seoul 06136, South Korea Phone +82 2 555 1988 Fax +82 2 555 8890 [email protected]
SpainMiltenyi Biotec S.L. C/Luis Buñuel 2 Ciudad de la Imagen 28223 Pozuelo de Alarcón (Madrid) Spain Phone +34 91 512 12 90 Fax +34 91 512 12 91 [email protected]
United KingdomMiltenyi Biotec Ltd. Almac House, Church Lane Bisley, Surrey GU24 9DR, UK Phone +44 1483 799 800 Fax +44 1483 799 811 [email protected]
www.miltenyibiotec.com
130-
118-
919.
02
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Copyright owl image: © Miles Away Photography/Shutterstock.com
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, MACSQuant, REAfinity, and Vio are registered trademarks or trademarks of Miltenyi Biotec GmbH and/or its affiliates in various countries worldwide. Copyright © 2018 Miltenyi Biotec GmbH and/or its affiliates. All rights reserved.
