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The
m i croguards
Source: www.bioetanoldecana.org - Adapted
Land use in Brazil
Total land area (851 Mha, 100%)
Agricultural properties area (355 Mha, 42%)
Cropland area (76.7 Mha, 9%)
Sugarcane cropland for energy-use area(3.6 Mha, 0.5%)
OVERVIEW
The
m i croguards
Raw Material EnergyProduced/Utilized
Avoided emissions
Sugarcane 9.3 89%
Corn 0.6 – 2.0 -30% to 38%
Wheat 0.97 – 1.11 19% to 47%
Beet 0.97 – 1.11 35% to 56%
Cassava 1.6 – 1.7 63%
Source: www.bioetanoldecana.org
Raw material utilized to produce ethanol
OVERVIEW
The
m i croguards
Source:Statistical Review of world energy 2009 - Adapted
Global ethanol production
OVERVIEW
The
m i croguards
Good substitutes for oil-based fuels
Solve the environmental problems
OVERVIEW
Biofuels
The
m i croguards
Enable appropriate land use
OVERVIEW
Scientific research
Should continue - obtain more efficient biofuels using genetic
engineering
The
m i croguards
First Mechanism (increased lactate sensibility)
Second Mechanism (recognizing lactate on the interior of the cell)
Biobrick submitted! Biobrick submitted!
Recognitionsystem
The
m i croguards
Successful assembled biobrick
BBa_K284002: JEN1 Promoter
BBa_K284003: Partial DLD promoter
BBa_K284023: EYFP regulated by ADH1 promoter (characterization device)
BBa_K284001: Lysozyme from Gallus gallus
BBa_K284016: Lysozyme constitutive expression (characterization device)
BBa_K284017: Lysozyme under control of DLD promoter (characterization device)
THEYeastguARdresults
The
m i croguards
Briefly,
If contaminants conjugate with our coliguard:
1. Induces Py
2. Releases AI2
3. Activate Differentiation and Killing mechanism
If contaminants release AI2 themselves:
1. .
2. .
3. Activate Differentiation and Killing mechanism
skip steps!
Recognitionsystem
The
m i croguards
Worker lineage characteristics!
The slippage mechanism controlling the basal proportions
(AGTC)10
Differentiationsystem
The
m i croguards
The slippage mechanism controlling the basal proportions
(AGTC)10(AGTC)9
Killer lineage characteristics!
Differentiationsystem
The
m i croguards
Integration between the mechanisms of slippage and Cre-Recombinase
Slippage error doesn’t occurs (most cases):
Cell Cycle!
Conjugation Inhibition System
Worker lineage
Differentiationsystem
The
m i croguards
Killer lineage
Integration between the mechanisms of slippage and Cre-Recombinase
Slippage error occurs (few cases):
(AGTC)9
Differentiationsystem
The
m i croguards
Killingsystem
The Kamikaze System
The Colicin System
CeaB lethal gene (colicin E2) into F plasmid
CeiB antidote gene into genomic DNA
The
m i croguards
Successful assembled biobrick
BBa_K284008: Py promoter + RFP device (characterization device)
BBa_K284031: Cre-Recombinase without ATG start codon
BBa_K284022: T4 endolysin under control of T7 promoter (characterization device)
BBa_K284022
THE COLIGUARD REsults
Characterized parts
The
m i croguards
The coliguardresults
Characterizing BBa_K284022
Transformations into E. coli strain C43 (T7 promoter is induced by IPTG)
Grown inocula were diluted to starter OD=0,2
Once OD=0,8 was reached induction with IPTG
Incubation for 4 hours at 37⁰C
The
m i croguards
Characterizing BBa_K284022
Plated each culture into solid LB-AMP media
The coliguardresults
The
m i croguards
Summary
2 contamination control models designed, one for prokaryotes and one for eukaryotes
30 new biobrick parts and devices designed
9 new biobrick parts and devices constructed and submitted to the registry
4 new biobrick parts or devices were tested
1 new biobrick device worked as expected (BBa_K284022)
An existing Biobrick part was characterized (BBa_K112806)
A new approach to an issue of Human Practice in synthetic biology as it relates to our project was outlined and detailed
Accomplishments
The
m i croguards
Special Acknowledgements:
Prof. Dr. Fernando Costa. Dean, UNICAMP.
Prof. Dr. Luís Cortez. CORI, UNICAMP.
Prof. Dr. Fábio Papes. Dept. Genética, Evolução e Bioagentes, UNICAMP.
Prof. Dr. Paulo Arruda. Dept. Genética, Evolução e Bioagentes, UNICAMP.
Prof. Dr. Gonçalo A. G. Pereira. Dept. Genética, Evolução e Bioagentes, UNICAMP.
Support: