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The LC-MS/MS WorkhorseSCIEX 4500 Series Mass Spectrometers
The SCIEX 4500 Series takes the legendary API 4000™ platform and intelligently re-engineers it to set a new benchmark for reliable quantitation. It offers the quantitative sensitivity, robustness, and performance you need, powered with QTRAP® technology to open a world of valuable experiments beyond MRM for significant data quality improvements for your most challenging methods.
Whether your research is focused on ADME, regulated bioanalysis, food and environmental contaminant screening, targeted quantitative proteomics, or clinical research, the SCIEX 4500 Series covers your key performance criteria:
• Quantitative and qualitative sensitivity to detect low concentration analytes in challenging matrices
• Dynamic range to see low to high concentration levels
• Scan speed to clearly resolve similar analytes
• Ionization source flow range for liquid chromatography flexibility
• Uniquely designed to occupy less of your valuable lab space
All backed by the integrity and quality of the SCIEX brand.
SCIEX 4500 Series The LC-MS/MS workhorse, intelligently re-engineered.
Redesigned from the foundation of our industry standard triple quadrupole mass spectrometers, the
4500 Series introduces a new era of LC-MS/MS performance defined by fast, precise quantitation and
ultra-fast scan speeds. The system is ideal for UHPLC, and with available QTRAP technology you get
better data, and more of it, than you can achieve using basic multiple reaction monitoring (MRM) on
an ordinary triple quad system.
Fast LC demands short dwell times
Delivering dwell times as low as 1ms, the 4500 complements
UHPLC separations to screen for more compounds within a single
experiment than ever before. You can maximize your capacity
and deliver quality quantitation every time using the powerful
Scheduled MRM™ algorithm which automatically optimizes your
acquisition method.
Better data. Complete confidence. Unrivalled efficiency. Ultimate performance.
Only SCIEX offers triple quads equipped with Linear Accelerator™
Trap technology. The QTRAP® 4500 delivers up to a 100-fold
increase in full scan MS/MS sensitivity enabling powerful workflows
that will give you a new level of confidence in your data.
• Unique scan functionality available only on the QTRAP, allows
you to see beyond the matrix to more accurately detect,
quantify, and confirm your compounds
• Acquire a complete MS/MS spectrum to accompany your
MRM quantitation for every compound detected in your
samples and cross reference with an integrated library for
ultimate confirmation
• Screen for more compounds in each analysis, without
compromising data quality for higher throughput
RUO-MKT-03-0137-C
For Research Use Only. Not for use in diagnostic procedures.
Much More Than a Triple QuadThe 4500 Series is robustly designed to deliver reliable quantitation over long runs, and is the only
triple quad system in the world equipped with Linear Ion Trap (LIT) technology that can be unlocked
to deliver enhanced capabilities when you need it.
QTRAP® technology delivers better data, and more of it, than you can achieve using basic multiple
reaction monitoring (MRM) on an ordinary triple quad system.
Diversity delivered
With the quantitative performance of a triple quad system
and QTRAP’s additional enhanced scan functionality, you are
able to develop new methods and improve results for your
existing workflows.
See more clearly beyond the matrix
When your analysis suffers from matrix effects and you’re
concerned about the accuracy of your MRM quantitation,
QTRAP technology can reduce the worry. The integrated Linear
Ion Trap (LIT) enables more accurate detection, quantification,
and confirmation of your compounds – without added laborious
or time consuming sample prep.
More confidently identify your compounds
For borderline MRM ion ratios, resulting in ambiguous
identification, there is a solution. QTRAP’s enhanced product
ion (EPI) functionality allows you to acquire a complete
MS/MS spectrum to accompany your MRM quantitation for
every compound detected in your samples. Cross reference this
‘compound fingerprint’ with an integrated library, and deliver
ultimate confirmation and report your analysis without doubt.
More compounds and more samples
If your workload increases, but your time and resources don’t,
QTRAP delivers more results in every run. The unique ability to
capture MRM and enhanced product ion confirmation scans
in one injection, without the need for long and inefficient
chromatography, enables reliable screening for more compounds
in each analysis, without compromising data quality. This all leads
to better throughput, without investing more time and resources.
Definitive Quantitation and So Much More
For Research Use Only. Not for use in diagnostic procedures.
The Future Path of LC-MS/MS Quantitation
The SCIEX 4500 Series brings together the latest hardware
from the world’s best selling triple quadrupole family,
and adds next-generation, ultra-fast and sensitive Linear
Accelerator™ Trap functionality. Delivering unmatched
quantitative and qualitative analysis, the 4500 Series
enables productive, time-saving workflows unachievable
with other mass spectrometry systems.
Patented QJet® Ion GuideOptimized design yields better ion containment and operates at high
pressure. This provides better collisional focusing to enhance ion
transmission for improved sensitivity. The new design also lets the
turbopump run cooler and in its ideal operating range.
AcQuRate™ Pulse Counting Detector
The AcQuRate™ pulse counting detector combined with a pulse overlap
correction algorithm, enable more accurate and precise ion detection over
a wide dynamic range. Continuous operation at maximum gain drives the
detector into the digital domain, simplifying the elimination of electronic
noise and guaranteeing maximum sensitivity with unparalleled accuracy
and precision.
Proven Q0 High-Pressure CellThe high pressure collisional focusing technology maximizes transmission of
ions for superior sensitivity.
Ions can be accumulated in the Q0 region while the Linear Accelerator™
trap is performing MS/MS and MS3 scans. This yields superior sensitivity
in ion trap mode, which can be extremely important for fast UHPLC
applications where time and duty cycle are condensed.
Patented Qurved LINAC® Collision Cell The Qurved LINAC® high-pressure collision cell accelerates ions
through the cell to increase speed of analysis and eliminate cross-talk.
Improvements to the acclaimed LINAC collision cell result in shorter transit
times across the cell, making the Qurved LINAC cell an ideal match for
UHPLC and high throughput analysis focused on hundreds of compounds.
True collision-induced fragmentation with the Qurved LINAC collision cell
generates reliable, information-rich, library-searchable MS/MS spectra time
after time.
Fast eQ™ Electronics for Fast LCNext-generation eQ™ electronics produce polarity switching in 50 ms
and scan speeds of 20,000 Da/s. Now, compounds with vastly different
functional groups can be measured in a single pass. The new electronics
also improve ion containment for better sensitivity and superior detector
performance. Ultra-fast and ultra-stable instrumentation means you get
the most out of your standard or fast LC to save time and accelerate
your research.
Integrated QTRAP® technology LINAC® technology to the Q3 linear ion trap greatly improves extraction
efficiency to yield up to a 100X gain in sensitivity under ion trap scan modes.
Take full advantage of the 20,000 Da/s scan speeds with full scan linear ion
trap sensitivity – 100X more sensitive than triple quad full scan experiments
for greater confidence in qualitative workflows. Improved excitation efficiencies
and reduced ion cooling and fragmentation times produce superior MS3
qualitative results and provide unprecedented selectivity for the most
challenging analytical assays.
RUO-MKT-03-0137-C
For Research Use Only. Not for use in diagnostic procedures.
Robustness and Performance for Any Application
With dwell times as low as 1ms, the 4500 series is ideally suited for multi component contaminant analysis as these data demonstrate with the detection of over 200 pesticides at 1ng/mL. The faster electronics and the intelligent Scheduled MRM (sMRM), permits the screening of hundreds of pesticides in a UHPLC time scale.
Superior quantitative performance for sensitive, accurate, and reliable analysis in antiretroviral drug research.
Without compromising sensitivity, the SCIEX QTRAP 4500 System has improved in every category vs. the API 4000 including dynamic range, scan speed and footprint.
Highly multiplexed peptide quantitation using the Scheduled MRM™ algorithm. 30 selected MRM transitions from a 4000 MRM assay of tryptic peptides. This experiment was run in replicate on an Eksigent 200 um ID cHiPLC® column with peak area deviation < 10%. Insert: an example of multiple MRMs to a single peptide, showing selected fragment ion chromatograms.
Contaminant analysis Drug monitoring research
Drug discovery and development Peptide quantitation
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0
Time, min
0.001.00e42.00e43.00e44.00e45.00e46.00e47.00e48.00e49.00e41.00e5
Inte
nsity
, cps
4.10
204 pesticide standard (1 ng/mL)
Lamivudine
Emtricitabine
Didanosine
Stavudine
Zidovudine
QT4500 spike6 NRTI.rdb (FTC 1): "Linear" Regression ("1 / x" weighting): y = 0.00204 x + -0.00116 (r = 0.9988)
0 500 1000 1500 2000 2500 3000 3500 4000 4500 5000Analyte Conc. / IS Conc.
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
An
aly
te A
rea
/ I
S A
rea
!"#$%&%#'(%)*+,-./0102223)45"6
QT4500 spike6 NRTI.rdb (FTC 1): "Linear" Regression ("1 / x" weighting): y = 0.00204 x + -0.00116 (r = 0.9988)
0 500 1000 1500 2000 2500 3000 3500 4000 4500 5000Analyte Conc. / IS Conc.
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
10.0
10.5
An
aly
te A
rea
/ I
S A
rea
Emtricitabine (FTC)5-5000 ng/mL
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8
Time, min
3.3e63.2e63.1e63.0e62.9e62.8e62.7e62.6e62.5e62.4e62.3e62.2e62.1e62.0e61.9e61.8e61.7e61.6e61.5e61.4e61.3e61.2e61.1e61.0e69.0e58.0e57.0e56.0e55.0e54.0e53.0e52.0e51.0e50.0e0
Inte
nsity
, cps
0.548
API 4000 Triple Quad™ 4500 system
Time, min Time, min
Inte
nsity
Inte
nsity
2.5e52.4e52.3e52.2e52.1e52.0e51.9e51.8e51.7e51.6e51.5e51.4e51.3e51.2e51.1e51.0e59.0e48.0e47.0e46.0e45.0e44.0e43.0e42.0e41.0e40.0e0
2.5e52.4e52.3e52.2e52.1e52.0e51.9e51.8e51.7e51.6e51.5e51.4e51.3e51.2e51.1e51.0e59.0e48.0e47.0e46.0e45.0e44.0e43.0e42.0e41.0e40.0e0
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
1.0 1.4 1.8 2.2 Time, min
5e4
Inte
nsity
, cps
Q3 m/z 416 725 1423 1698 1816
10 15 20 25 30 35
Time, min
100
Inte
nsity
, cps
For Research Use Only. Not for use in diagnostic procedures.
QTRAP® 4500 System
MRM3—Enhanced selectivity
Reactive metabolite screening
High confidence library searching capabilities
Qual-Quant: detection and confirmation
Quantitation of tryptic peptides in a complex matrix. Higher selectivity workflows such as MRM3 (bottom) can provide additional specificity for low level detection of compounds when high background or interferences render MRM workflows ineffective (top).
Combining intelligent peak detection with the ability to trap and scan out ions on a UHPLC time scale, creates a potent solution for detecting unexpected contaminants. Add to this a single library of thousands of QTRAP spectra for library matching, and contaminants at the lowest levels can be confidently identified.
Troglitazone GSH adduct was detected using complimentary positive neutral loss and negative precursor ion scans, combined with Information Dependent Acquisition (IDA) linear ion trap scans. This unique workflow results in the comprehensive detection and characterization of GSH adducts in a single injection.
The QTRAP 4500 system allows Scheduled MRM (sMRM) to trigger high quality, full scan product ion spectra for use in library searching. With highly sensitive Triple Trap™ scanning it is possible to confidently identify compounds at up to 100X lower concentrations than triple quadrupole full scans.
20.6 21.0 21.4 21.8
Time, min
10021.1
21.36
MRM3
Sum of fiveMS/MS/MS ions
Inte
nsity
, cps
In
tens
ity, c
ps
100
21.1
MRM
2y52y42y6
80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280
m/z, Da
0.00
5.00e4
1.00e5
1.50e5
2.00e5
2.50e5
3.00e5
3.50e5
4.00e5
4.50e5
5.00e5
5.50e5
6.00e5
6.50e5
7.00e5
7.50e5
8.00e5
8.50e5
9.00e5
9.50e5
1.00e6
Inte
nsity
, cps
160.1
192.1
132.0105.0
117.0 134.090.0 106.0 149.092.0
Compound identification with high confidence using MS/MS library searching
67 ng/mL Carbendazim in orange juice (FIT = 97%)
Inte
nsity
Inte
nsity
Inte
nsity
735 740 745 750 755 760 765 770 775 780 7850e01e52e53e54e55e56e57e58e59e5 747.3
748.3
749.3
750.3
1.17
3.77
100 150 200 250 300 350 400 450 500 550 600 650 700 750
Mass/Charge, Da
0.0e02.0e64.0e66.0e68.0e61.0e71.2e71.4e71.6e71.8e72.0e72.2e7 308.1
179.1162.0
233.1
747.3309.1217.1440.2143.9
234.9 618.3
+NL of 129 and – Prec of m/z 272 scans with fast polarity switching
Enhanced resolution
Enhanced product ion scan obtained in IDA mode
Time, min
Mass/Charge, Da
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5
5.5e55.0e54.5e54.0e53.5e53.0e52.5e52.0e51.5e51.0e55.0e4
0e0
0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5Time, min
0.0
5.0e5
1.0e6
1.5e6
2.0e6
2.5e6
3.0e6
3.5e6
4.0e6
4.5e6
5.0e6
5.5e6
Inte
nsity
, cps
• 6 minute run time • 2.2 mL/min flow rate • Monitoring 256 drugs • ~5 sec wide peaks
60 80 100 120140 160180200220 240 260 280300320340360380400420440460 4800.0
1.0e4
2.0e4
3.0e4
4.0e4
5.0e4
6.0e4
7.0e4
8.0e4
9.0e4
1.0e5
1.1e5
1.2e5
1.3e5
1.4e5 337.2
188.2
105.2
Fentanyl
sMRM survey scan for Quant
60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 4800.0
5000.0
1.0e4
1.5e4
2.0e4
2.5e4
3.0e4
3.5e4
4.0e4
4.5e4
5.0e4
5.5e4
6.0e4
6.5e4
7.0e4
7.5e4
8.0e48.2e4 265.2
310.2
104.9
159.1219.2116.9
203.3167.2
Methadone
60 80 100 120 140 160 180 200 220 240 260 280 300 320 340
m/z, Da
0.0
1.0e5
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
7.0e5
8.0e5
9.0e5
1.0e6
1.1e6
1.2e6
1.3e6
1.4e6
Inte
nsity
, cps
m/z, Da
Inte
nsity
, cps
m/z, Da
Inte
nsity
, cps
302.2
284.2
227.2
198.2
242.2161.0181.1
173.3224.2
214.2146.2 188.2 256.2201.1 266.2240.2153.2 211.6144.0132.1
Oxymorphone
Example Triggered Product Ion Spectra For Library Searching
QTRAP® Technology – Unmatched Versatility and Confidence
RUO-MKT-03-0137-C
The excellent reproducibility
for the peak area ratio of
Methamphetamine and
Amphetamine in human
plasma shows the stability
of the AcQuRate™ pulse
counting detector for
consistent quantification
Ultimate sensitivity and simplicity
The patented QJet® ion guide improves
ion containment and operates at high
pressure, providing better collisional
focusing to enhance ion transmission for
ultimate sensitivity. The proven design
also reduces the gas load, allowing the
turbopump to run cooler in its ideal
operating range. It all adds up to our
most reliable system – and with tool-free
maintenance, clean-up is simple
and straightforward.
Nanoflow made easy
The NanoSpray® III ion source makes
working with nanoflow chromatography
easy while providing the highest sensitivity
and stability. The NanoSpray III source
supports regular and column-packed
emitter tips for ultimate chromatographic
flexibility. The camera design allows
clear spray visualization for simplified
optimization. Fingertight fittings enable you
to change tips quickly, so you are up and
running in no time.
Drive productivity
The Turbo V™ source provides
high-sensitivity analysis over a wide range
of flow rates with quick-change APCI and
TurboIonSpray® probes. From 50 μL/min
to 3 mL/min, the Turbo V Source is the
perfect match for narrow bore, standard
bore and UHPLC flow rates, delivering
unprecedented desolvation and stability for
even the toughest high-flow applications.
Injection Number
Robustness Test in Plasma: 1500 Injections
Res
po
nse
Pea
k A
rea
(co
un
ts)
For Research Use Only. Not for use in diagnostic procedures.
One Touch ProductivityTake full advantage of all the speed and power of the 4500 Series. Powerful, workflow-driven
software ties everything together efficiency, throughput, and productivity. Industry-standard Analyst®
Software utilizes the intelligent Scheduled MRM™ algorithm to make the method setup of over
1000 analytes in a single LC analysis straightforward and simple while still generating exceptional
quantitative and brilliant qualitative results.
Instrument control, analysis, and reporting
Analyst® Software
provides state-of-the-art
functionality for instrument
control, data analysis, and
reporting. The latest version builds on this legacy by providing
new features that enhance both performance and ease of use.
Save time, without compromising
Complete metabolite coverage
LightSight® Software
simplifies analysis of
complete metabolite
coverage. Create expert-
level acquisition methods in
just a few simple steps using the automated method development
tool. Alternatively, take advantage of customized glutathione
screening to quickly identify potential reactive metabolites and
significantly increase metabolite detection with targeted methods.
MRM data processing
MultiQuant™ Software is
a powerful, easy-to-use
package that processes
MRM data for quantitative
information. The software
handles large data sets
consisting of both large numbers of MRM transitions and study
samples, with an emphasis on the requirements for processing
protein/peptide quantitative workflows. Results are easily exported
to other software packages, or use the software’s flexible reporting
features to generate custom reports.
Fast multi-component screening
With a simplified user-
interface and streamlined
workflows, MasterView™
Software enables you to
process your QTRAP® MS/
MS screening data faster, with improved confidence beyond MRM
detection alone. The integration of MS/MS compound libraries and
customizable data review settings, screen for more compounds in
more samples faster and more reliably than ever before– reducing
your data processing time to minutes. MasterView Software is
ideal for routine food, environment, and forensic labs testing high
volumes of samples in complex matrices and requiring the highest
confidence in compound identification.
RUO-MKT-03-0137-C
AB Sciex is doing business as SCIEX.
© 2016 AB Sciex. For Research Use Only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license.
10/2016 RUO-MKT-03-0137-C
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