Upload
oscar-walters
View
221
Download
0
Embed Size (px)
DESCRIPTION
Subject & Methods 40 eyes of 20 New Zealand White rabbits Bilateral study Corneal epithelial flap(diameter of 5.0 mm) was made using the following ways: Group A ; 1. A pplication of 20% alcohol for 30 seconds, 2 or 3 times 2. Corneal epithelial flap was made using a micro hoe (Katena, U.S.) 3. The flap was replaced Group B ; 1.The flap was made using epimicrokeratome(Amadeus, 나라 Hz) 2. The flap was replaced
Citation preview
The expression of inflammatory cytokines in chemical- or microkeratome-assisted corneal epithelial flaps in rabbits
Taehyung Lim, MD1,2,4, Hyuk- Jin Choi MD1,2, Hyun Joo Lee1,2, Mee Kum Kim, MD1,2, Won Ryang Wee, MD1,2, Jin Hak Lee,2,3
Department of Ophthalmology, Seoul National University Hospital1, Seoul, KoreaSeoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute2, Seoul, KoreaDepartment of Ophthalmology, Bundang Seoul National University Hospital3, Seoul, KoreaHanGil Eye Hospital4, Incheon, Korea
Authors have not any financial support
Purpose
To compare the expression of inflammatory cytokines in chemical- or microkeratome-assisted corneal epithelial flaps in rabbit animal models.
Subject & Methods 40 eyes of 20 New Zealand White rabbits Bilateral study
Corneal epithelial flap(diameter of 5.0 mm) was made using the following ways: Group A ; 1. Application of 20% alcohol for 30 seconds, 2 or 3 times 2. Corneal epithelial flap was made using a micro hoe (Katena,
U.S.) 3. The flap was replaced Group B ; 1.The flap was made using epimicrokeratome(Amadeus, 나라
Hz) 2. The flap was replaced
All the eyes wore therapeutic contact lenses and received tarsorrhaphies
Eyes were enucleated at 3 days after the procedure
Tissue sections were stained with H&E and immunohistochemistry (MMP-9, TNF-alpha)
(hamster anti mouse TNF-alpha : Santa Cruz, sc-12744, mouse anti MMP-9, Chemicon, MAB3309)
IL-6, TNF-alpha were evaluated by RT-PCR and were compared between those two groups.
(Rabbit TNF-a ; Forward PRIMER : atggtcaccctcagatcagc Reverse PRIMER : ttgaccgctgaagagaacct, Rabbit IL-6 ; Forward PRIMER : tcctggagaccatcaaggagReverse PRIMER : gggtggcttcttcattcaaa )
Results
H&E stain showed more irregular arrangement of epithelial basal cells in Group A than in Group B
Normal Group A Group B
RT-PCR
- The expression level of mRNA of IL-6, MMP-9, TNF-alpha showed no significant difference between those two groups
Mean ± SD
Immunohistochemistry
MMP-9
Negative Control, x 200 Positive Control, x 200
MMP-9 (x 200)
Group A
Group B
Immunohistochemistry TNF-alpha
Negative Control, x 200 Positive Control, x 200
TNF-alpha (x400)
Group A
Group B
MMP-9 and TNF-alpha were expressed and were more prominent than negative control tissue in all groups
There was no significant difference between two groups
Conclusion
The expression of inflammatory cytokines in epimicrokeratome-assisted flap might be no different from alcohol-assisted flap, suggesting that the inflammatory response of the cornea might be similar between Epi-LASIK and LASEK.