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The comparing of natural antibacterial substance on E. Coli. Manhasset Science Research Jean Cheng Period 4. Need. Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil. http://www.scielo.br/img/revistas/bjm/v39n4/arq27_fig01.jpg. Knowledge Base. - PowerPoint PPT Presentation
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The comparing of natural antibacterial substance on E. Coli
Manhasset Science ResearchJean Cheng Period 4
http://www.scielo.br/img/revistas/bjm/v39n4/arq27_fig01.jpg
Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil
Need
Figure 2: Chemical formula for Penicillinhttp://itech.dickinson.edu/chemistry/wp-content/uploads/2008/04/penicillin-core1.png
Knowledge Base
Figure 2: Chemical formula for Penicillinhttp://itech.dickinson.edu/chemistry/wp-content/uploads/2008/04/penicillin-core1.png
Knowledge Base
Figure3: Penicillin attacking the peptidocglycan http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Gram_negative_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png
Knowledge Base (cont)
Peptidoglycan
Knowledge Base (cont)
Figure3: Penicillin attacking the peptidocglycan http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Gram_negative_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png
Peptidoglycan
Figure 4: Tea tree’s terpene chemical formula
http://www.wacker.com/cms/appL/mdm_images/R67_IV-1_-432011277_8959.jpg
Figure 5: Pectin Chemical formula
http://sci-toys.com/ingredients/pectin_2.gif
Knowledge Base (cont)
Figure 6: Aloe verahttp://apps.who.int/medicinedocs/fr/d/Js2200e/5.html
Knowledge Base (cont)
Knowledge Base (cont)
Figure 7: Citralhttp://www.impgc.com/images/Structures/Citral.bmp
Figure 8:Chemical Change in Allicin subjected to different conditions. (Ariga, et al. 2008)
http://media-2.web.britannica.com/eb-media/33/7433-004-96DFD197.gif
Literature Review
Figure 9: Antimicrobial activity of aloe vera gel
(Alemdar, et al. 2009)
Literature Review (cont)
Figure 10:Yuzu fruit seed oil on different microbial
(Nakazono, et al. 2006)
Literature Review (cont)
Figure 11:12 different essential oil on different species of bacteria.
(Rusenova, et al. 2009)
Literature Review (cont)
Find which natural substance is best in inhibiting E. coli.
Null Hypothesis: No effectAlternative Hypothesis: Tea tree oil is the
best antimicrobial substance against E. coli
Purpose
The comparing of natural antibacterial substance on E. Coli
Essential oil and antibiotic plant substanceN=15
Garlic (allicin)N=3
Tea Tree oilN=3
Petri dish, E.coli, filter paper, glass spreader
Disc diffusion assay method
SPSS ANOVA Test
Aloe (anthraquinones)N=3
Yuzu fruit oilN=3
Millimeter
Methodology
Ginger (citral)N=3
Bacteria on agar
Inhibited Zone(mm)
Paper disk with substance being tested
Figure 12: Disk Diffusion method
http://diverge.hunter.cuny.edu/~weigang/Images/20-17_diskdiffusion.jpg
Protocols
Figure 13: http://gsbs.utmb.edu/microbook/images/fig10_5.jpg
Protocols (cont)
Not expensivedoesn’t require large amount of spaceSome materials found in lab or
supermarketE. coli is needed as well as agar, aloe, tea
tree, yuzu, and garlicNatural antiseptic doesn’t have negative
effect
Do-Ability
Vendor Cat# pg Item Qty Unit$ Total$Carolina 124400 Escherichia coli, Living, 5 mL 1 $9.20 $9.20Carolina 821040 Tryptic Soy Agar Media Kit 1 $26.95 $26.95Carolina 703413 Glass Spreader 1 $4.70 $4.70Ward 86 V 5900 Aloe Living Specimen 1 $7.95 $7.95Vitamin Shoppe Australian Tea tree Oil 1 7.99 $7.99
$56.79
Budget
•Aiello, Castoki. Consumer Antibacterial Soaps: Effective or Just Risky?. The Infectious Disease Society of America Journal. Vol 10, p1058-1078, 2007
•Aljady, A. Biochemical Study on The Efficacy of Malaysian Honey on Inflicted Wounds: An Animal Model. Medical Journal of Islamic Academy of Sciences. Vol 13 #3. pg 125-132, 2000
•American Chemical Society. "Love That Garlic? Fresh May Be Healthier Than Bottled." ScienceDaily 12 June 2008. 7 May 2009 <http://www.sciencedaily.com /releases/2008/06/080609092230.htm>.
•Amagase, H., Intake of garlic and its bioactive components. Nutritional Journal. Vol131(3S) pg 955S-962S, 2001
•"antiseptic." Grolier Multimedia Encyclopedia. 2009. Grolier Online. 3 May 2009 <http://gme.grolier.com/cgi-bin/article?assetid=0013590-0>.
•Avallone, Eugene A., ed. "Antibiotic." McGraw-Hill Encyclopedia of Science and Technology. 8th ed. 20 vols. United States of America: Library of Congress, 1960. 756-67.
•Brunner, Nasco. Do Bioactive Glasses Convey A Disinfecting Mechanism Beyond A Mere Increase in pH?. Internation Endodontic Journal, Vol 41(8) p670-678, 2008
•Carr, Anitra. Therapeutic Properties of New Zealand and Australian Tea Trees. New Zealand Journal, Vol 18#2, pg 47-51. 2009
•Chen, Tao. Triclocarban Enhances Testoterone Action: a New Type of Endocrine Disruptor?. Endocrinology journal. Vol 1. p 10-27, 2007
•Cuttle, Leila. The efficacy of Aloe vera, tea tree oil and saliva as fist aid treatment for partial thickness burn injuries. Elsevier Journal. Vol 1. pg 1176-1182. 2008
•Davis. D. "Antibacterial Chemical Disrupts Hormone Activities, Study Finds." ScienceDaily 8 December 2007. 3 May 2009 <http://www.sciencedaily.com /releases/2007/12/071207150713.htm>.
Bibliography
•Freeman, Frachesco. Garlic chemistry: stability of s-(2-propenyl)-2-propene-1-sulfinothioate (allicin) in blood,
solvents, and simulated physiological fluids. J. Agric. Food Chem. Journal. Vol 43 pg2332-2338, 1995.
•Fulder, Stephen. Garlic: nature's original remedy. 2nd ed. United States: Inner Traditions / Bear & Company,
2000.
•Ingram, Cass. Tea Tree Oil: The Natural Antiseptic. 1st ed. United States: Knowledge House, 1999.
•Koizum. Love That Garlic? Fresh May Be Healthier Than Bottled : Diallyl sulfides in garlic activate both TRPA1
and TRPV1. Biochemical and biophysical research communications journal. Vol 19. p929-934, 2009
•Lawson L.D, Characterization of the formation of allicin and other thiosulfinates from garlic. Planta Medical
Journal. Vol 58. pg345-350, 1992.
•Macpherson, Casey. The Pungency of Garlic: Actiation of TRPA1 and TRPV1 in Response to Allicin. Current
Biology Journal. Vol 15, p929-934, 2005
•Nagane, N. Efficacy of Topical Honey Therapy Against Silver Sulphadizine Treatment in Burns; A biochemical
study.Indian Journal of Clinical Biochemistry,Vol 19#2. pg 173-176. 2004
•Nakazono, G. Studies on Antimicrobial and Antioxidative Substance of Yuzu (Citrus junos hort. ex Tanaka)
Seed. Journal of Biological Sciences. Vol 1. p 135-139, 2006
•Rusenova, Ishiwa. Antimicrobial Activities of Twelve Essential Oils Against Microorganisms of Veterinary
Importance. Trankia Journal of Sciences. Vol 7. p37-43, 2009
Bibliography (cont)
•Shashikanth, K.N. Effect of feeding raw and boiled garlic (Allium sativum l.) on the growth, caecal microflora
and serum proteins of albino rats. Nutrition Reports Int. Journal. Vol33(2) pg 313-319. 1986
•Society for General Microbiology. "Tea Tree Oil And Silver Together Make More Effective Antiseptics."
ScienceDaily 6 April 2009. 3 May 2009 <http://www.sciencedaily.com /releases/2009/03/090329205453.htm>.
•Tao. Cho. Chemical Composition of Essential Oil From The Peel of Satsuma Mandarin. Journal of Natural
Sciences. Vol 7(9), p1261-1264,2008
Bibliography (cont)