The Biotechnology Center of ExcellenceBringing Biotech from the Bench to the K-12 Classroom

Event: Regenerative Medicine WebinarDate: May 22, 2014, 4pm & 7pm CSTLocation: Lindblom Math & Science Academy

Hortense Brice, Presenterhebrice@gmail.com

Dr. Emily Ferrin, Facilitatoremily.ferrin@northwestern.edu

BCoE.netosep.northwestern.edu

Webinar Objectives As a Result of Webinar Participation, You

Will:1.Realize the Need for Regenerative Medicine

Practices and Procedures

2. Identify the Tools and Technologies of Regenerative Medicine

3.Understand the Challenges, Promise and Potential of Regenerative Medicine

4. Identify the Potential Benefits of Regenerative Medicine to America’s Costly Health Care System

“Body Builders”: The Emerging Science of Regenerative

Medicine

The Beginning………

Charles Vacanti & Robert Langer (1997)

Characterizing Regenerative Medicine

1. Regenerative medicine is a broad definition for innovative medical therapies that will enable the body to repair, replace, restore and regenerate damaged or diseased cells, tissues and organs. (Mayo Clinic)

2. Science Merges with Technology to Achieve Self-Healing• Regenerative medicine uses procedures to repair or replace damaged or diseased

tissues and organs—Many traditional therapies only treat symptoms.

3. Tools and Procedures (Biofabrication or Additive Manufacturing) of Regenerative Medicine

• Tissue Engineering: Tissue Repair/Replacement and Lab Grown Organs• Technologies Stem cells Natural and Synthetic Scaffolds 3-D Printing and Chip Technologies

4. Highly Interdisciplinary Field where Biology and Chemistry meet:• Engineering• Material Science

Areas of Regenerative Medicine

1. Artificial Organs: Medical Devices

(Lab Grown Bladder)

2.Tissue Engineering & Biomaterials

Scaffolds

Areas of Regenerative Medicine

3. Cellular Therapies• Use of Stem Cells (From Patient)• Development of Regenerative

Medicine Treatments.• Enhance Regeneration of Tissues

and Organs.

4. Clinical Trials• Many Currently in Progress.• NIH and Private Organizations.

Regenerative Medicine Procedures/Protocols

Engineer Scaffolds

•

HydrogelSpaghetti Highways

Ear Scaffold

Regenerative Medicine Procedures/Protocol

Seed Scaffold With Stem Cells

Regenerative Medicine Procedures/Protocol

• Incubate Seeded Scaffold in Bioreactor

• Transplant into An Animal Model

Regenerative Medicine And PRP Therapy

• PRP: Platelet Rich Plasma Therapy• Concentrate Platelets from Patients Blood

Sample• Platelets Contain Cytokines and Growth

Factors• PRP Accelerates Healing and Reduces

Pain• Bill Nye “The Science Guy” and PRP

Therapy

Regenerative Medicine Pioneer

Wake Forest Institute for Regenerative Medicine (WFIRM)

Dr. Anthony Atala

•First to Engineer/Transplant Lab-Grown Organ into a Human

•Transplant was Successful•Currently Developing Organ on a Chip Program

Regenerative Medicine Pioneer

Dr. Paolo Macchiarini (Karolinska Institute)

•2008: Implanted World’s First Donor Trachea•Recipient: Claudio Castillo•Survived Procedure—Now Has Normal Respiratory Function

Regenerative Medicine PioneerMcGowan Institute of Regenerative Medicine (University of Pittsburg)

Dr. Stephen Badylak•Removed Cells from Pig Bladder Extra Cellular Matrix (ECM)•Dried: Forms “Pixie-Dust”•Re-grows Severed Digits and New Muscle Tissue

Regenerative Medicine Pioneer

AFIRM: Armed Forces Institute Of Regenerative Medicine.

•Treatment Procedures for Severely Wounded Soldiers•Funded and Managed by US Army•Partnership with Wake Forest Institute for Regenerative Medicine

Regenerative Medicine Innovator

Dr. Geraldine Hamilton (Wyss Institute)

Organ on a Chip Technology(Drug Testing Tool)

Regenerative Medicine Innovator

Jordan Miller (Rice University)• Uses 3-D Print Technology• Engineers Blood Vessels Using Sugar

Regenerative Medicine Innovator

Ramille N. Shah, PhD (Northwestern University)

• Leader in Field of 3D-Printable Materials

• Engineers new 3D-Inks

• Creates Porous Scaffolds

• Technique: Additive Manufacturing

(Nanofiber Scaffold For

Cartilage Regeneration)

Regenerative Medicine Challenges1. Design of Biomaterials that Function in

the Body

2. Getting Enough Cells and Cell Types for Engineering Tissues and Organs

3. Vascularity: Engineering Blood Vessels that Supply Nutrients, Oxygen and Signals to Bioengineered Tissues and Organs

4. Cost of Tissue and Organ Development Procedures

Organ Decellularization Approach

Dr. Harald Ott

http://www.youtube.com/watch?v=5wfdhB_VyJw

Challenge To Regenerative Medicine

Bioengineering of Organs

Potential of Regenerative Medicine

Chip Technology

• Reduces Need for Animal Testing

• 3-D Printed Organs on Chips Used to Test Vaccines

3-D Elastic Membrane Fits Heart’s Epicardium

3-D Printer Creates Heart Membrane

Promise of Regenerative Medicine

The Business of Regenerative Medicine

Organovo3-D Bioprinting CompanyStarted A Collaboration with NIH (January 2014)Goal: Bioprinting of 3D Living Tissues • Eliminate Challenges to New Therapy

Development• Animal Models: Poor Predictors of Drug Efficacy

and Toxicity

The Business of Regenerative Medicine

TengionA Biotechnology Company Company Platform for Engineering

Tissues/Organs Organ Regeneration Processo Doctors send Tissue Sample from Diseased or

Failing Organ to Tengiono Tengion Selects and Multiplies Healthy Cellso Place Cells on an Organ-Shaped Scaffoldo Result: A Neo-Organ for Transplant

Futuristic!

Stem Cells + Organ Scaffold + 3D Printer

= Libraries of Replacement Organs?

Regenerative Medicine Summary

• Regenerative Medicine Approacheso Use of Patients Stem Cellso 3-D Bioprinting of Tissues and Organso Organ Decellularizationo PRP: Plasma Rich Platelet TherapyHuman TreatmentVeterinary Medicine Therapy

Regenerative Medicine And Healthcare

• New Era in Medicine: Move from Treatment to Cures (Self-Healing)

• Eliminate Organ Transplant Shortage

• Reduce Health Care Costs

• Now A Medical and Commercial RealityOver 60, 000 Stem Cell Transplants Worldwide2012: Cell Therapies Generated More Than

$900 Million

Thank you!

osep.northwestern.eduemily.ferrin@northwestern.eduhebrice@gmail.com