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The analytical laboratorySchool of Biological Sciences
Dr Bowater Prof. RichardsonInteractions of proteins with DNA The bacterial nitrogen cycle & anaerobic metabolism of bacterial pathogens
Dr Brearley Dr WatmoughPlant metabolic biochemistry Enzymology of Nitric oxide generation/metabolism Oxidases and peroxidases in aerobic metabolism Dr ClarkeBiophysical/spectroscopic analysis of protein-protein interactions
Dr HemmingsX-ray structural and mechanistic studies of enzymes
Catalytic properties of metalloproteins involved in bacterial respiration which enable bacteria
to exploit host cell environments in symbiosis and pathogenesis
The analytical laboratorySchool of Biological Sciences
Facilities & expertise
Protein expression.
Protein purification
Protein characterization
Chromatographic analysis/purification of small molecules
The analytical laboratorySchool of Biological Sciences
Protein purification
Under aerobic and anaerobic conditionsfrom Category 2 microorganismsLicense/authority from Defra, HSE
Protein purification on analytical, pilot and larger scales
Protein structure determinationX-ray crystallography with access to beam time on synchrotonsRobotic crystallization trials
Protein characterization - capabilities
Structure-informed protein characterization and rationale design is available
Protein structure prediction and modellingEnzymology, especially of metalloproteinsVariety of spectroscopic techniquesTime-resolved spectroscopy of metalloproteinsFast reaction kinetics
Analytical ultra centrifugationHydrodynamic properties of proteins
Protein characterization - capabilities
In absence of crystal structures, protein structures are modelled
Isothermal calorimetryto study protein-ligand interactions
True thermodynamic parameters for any reaction are obtained by ITC
0.0 0.5 1.0 1.5 2.0 2.5
-10
-8
-6
-4
-2
0
0 10 20
-0.4
-0.2
0.0
0.3 mg/ml (C12A)Mur + 1 mMMn(II)Time (min)
µca
l/sec
Molar Ratio
kcal
/mol
e of
inje
ctan
t
Metabolic and small molecule analysis
Gas Chromatography with liquid and headspace samplingElectron Impact GC-Mass Spectrometry Flame Ionization Detection Electron Capture Detection
Fermentation products, gases, natural oils are analyzed by GC
Metabolic and small molecule analysis
Tracer analysisStable isotopes and radioisotopesLicensed by Env. Agency
High Pressure Liquid Chromatograhy
on analytical and semi-prep scale
UV, DAD, suppressed ion conductivity, fluorescence &
radiochemical detection
HPLC-Mass Spectrometry
HPLC method development is available
Contact details & sponsors
Contacts
