TETON CONSERVATION DISTRICT Conservation District employees and field samplers will follow the guidelines set forth in this sampling and analysis plan, as well as those set forth in

TETON CONSERVATION DISTRICT

SAMPLING AND ANALYSIS PLAN FOR THE

FLAT CREEK MONITORING PROJECT

Created: January 2000 Revised: January 2003

SAMPLING AND ANALYSIS PLAN

Flat Creek/Flat Creek Sub-Watershed/Flat Creek Monitoring Project Teton Conservation District

Created: January 2000 Revised: January 2003

Project Supervisor – Randy Williams

Teton Conservation District (307) 733-2110 – Phone

(307) 733-8179 – Fax 230 East Broadway Suite 2A

P.O. Box 1070 Jackson, Wyoming 83001

Submitted by Brian E. Remlinger

Revised January 2003 Teton Conservation District Sampling and Analysis Plan – Flat Creek Monitoring Project

TABLE OF CONTENTS

Corrective Actions and Revisions to January 2000……………………………………….1 Signature Approval Sheets……………………………………………………………....2,3 Abstract……………………………………………………………………………………4 Purpose Statement……………………………………………………………………….5,6 SAMPLING Sampling Personnel and Training…...…………………………………………………….7 Sampling Design………………………………………………………………………..…8 Sampling Locations and ID Codes…………………………………………………….9,10 Proposed Sampling Schedule……….…………………………………………………....11 Parameters and Methods, Chemical…...……………………………………………...12,13 Parameters and Methods, Biological..…………………………………………………...14 Parameters and Methods, Physical………………………………………………………15 Health and Safety………………………………………………………………………...16 Corrective Actions………………………………………………………...……………..17 QUALITY ASSURANCE/ QUALITY CONTROL (QA/QC) Calibration and Maintenance…………………………………………………………….18 Quality Control Samples…………………………………………………………………19 Sample Testing/ Contract Laboratories……………………………………………….…20 Field Log Examples…………………………………………………………………..21,22


DATA Data Archiving…………………………………………………………………………...23 Data Review/Validation………………………………………………………………….24 Lab Results……………………………………………………………………………….24 Photographic Documentation…………………………………………………………….24 Landowners and Contact Information…………………………………………………...25 Report Generation..………………………………………………………………………26 Statistical Analyses………………………………………………………………………26 LIST OF TABLES Sampling Personnel……………………………………………………………………….7 Sampling Locations.…………………………………………………………………...9,10 Proposed Sampling Schedule…………………………………………………………….11 Parameters, Chemical………………………………………………………………...12,13 Parameters, Biological…………………………………………………………………...14 Parameters, Physical……………………………………………………………………..15 Calibration and Maintenance…………………………………………………………….18 Quality Control Samples…………………………………………………………………19 Sample Testing/ Contract Labs…………………………………………………………..20 Data Archiving…………………………………………………………………………...23 Landowners and Contact Information…………………………………………………...25


APPENDICES APPENDIX A Credible Data Legislation…………………………………………………………1 APPENDIX B - FORMS DEQ-WQD Watershed Program BURP Monitoring Field Data Sheets……..1 - 27 Chain of Custody Form……………………………………………………………1 Equipment Calibration and Maintenance Log (example)………...……………….1 APPENDIX C Sample Labeling (example)……………………………………………………….1 APPENDIX D Fish Creek Watershed Map………………………………………………………..1 Sampling Site Location Maps…………………………………………………1 – 8 APPENDIX E Standard Operating Procedures………………………………………………1 – 65 -Table of Contents Included in Appendix


Revised January 2003 Teton Conservation District 1 Sampling and Analysis Plan – Flat Creek Monitoring Project

Corrective Actions and Revisions January 2003

Submitted by Brian Remlinger

The January 2000 Sampling and Analysis Plan (SAP)- Flat Creek Montioring Project was developed and written by Rik Gay and Heather Alsup. At that time Larry Jorgenson was Chairman of Teton Conservation District and Steve Kleuss was District Conservationist with the Natural Resource Conservation Service. Revisions to the January 2000 SAP are a result of further monitoring site selection, personnel changes, equipment upgrades, water quality parameter modifications and unknowns discovered. The following are brief descriptions of the revisions to the January 2000 Flat Creek SAP: Pages 2-3 Personnel changes to signature approval sheet Page 7 Sampling personnel changes and training updates Page 9-10 Monitoring site updates including addition of Cache Creek and Game

Creek monitoring sites Page 14 Total coliform parameter changed to e. coli bacteria to comply with

WDEQ water quality standards Page 18 Multi Probe water analyzer replaced with Hach sensION 156

Global Flow Probe upgraded to Marsh-McBirney FLOMATE 2000

Page 19 Cache Creek and Game Creek samples added to Quality Control Samples

Page 25 Landowners and Contact Information updated to reflect new

monitoring sites

Appendix E Sample Site location Maps developed for Cache Creek and Game Creek


Signature Approval Sheet

Teton Conservation District Sampling and Analysis Plan – Flat Creek Monitoring Project

Name Randy Williams

Signature Title Executive Director, Project Supervisor

Organization Teton Conservation District

Name Kate Mead Signature Title Chairman Organization Board of Directors, Teton Conservation District

Name Jenny Castagno Signature Title District Conservationist Organization Natural Resources Conservation Service

Name Brian Remlinger

Signature Title Water Resources Specialist, Monitoring Supervisor Organization Teton Conservation District

Name Brian Lovett Signature Title Nonpoint Source Coordinator Organization Wyoming Department of Environmental Quality, Water Quality Division,

Watershed Program


Signature Approval Sheet

Teton Conservation District Sampling and Analysis Plan – Flat Creek Monitoring Project

Name Mike Foster Signature Title QA/QC Officer Organization Wyoming Department of Environmental Quality, Water Quality Division,

Watershed Program

Name Bob Wisseman Signature Title Senior Scientist Organization Aquatic Biology Associates

Name Ken McMillan

Signature Title Senior Scientist Organization Wyoming Department of Agriculture Laboratory

Name Ardith Moran Signature Title Branch Lab Supervisor Organization Department of Health and Welfare Lab – Pocatello, Idaho


TETON CONSERVATION DISTRICT SAMPLING AND ANALYSIS PLAN

FLAT CREEK MONITORING PROJECT

Abstract

The headwaters of Flat Creek are located within the Gros Ventre mountain range in the Bridger-Teton National Forest. From there, Flat Creek flows southwest across the National Elk Refuge and through the town of Jackson, all the while being subjected to various impacts. These impacts range from large amounts of fecal contaminants due to the wildlife habitat of the National Elk Refuge to increasing urban impacts such as road construction, sewer, and urban storm run-off. Flat Creek runs through a campground, residential, industrial and agricultural areas, and receives water from two separate diversions before its confluence with the Snake River. Residents of Teton County have voiced concern about the questionable health of Flat Creek. This water body was also listed on the Department of Environmental Quality’s 303d list of possibly impaired waterbodies. Because of these concerns, the Teton Conservation District is implementing a water quality monitoring project on Flat Creek in order to assess the quality of this water body.

The Teton Conservation District has utilized the public input process by inviting landowners along Flat Creek and other concerned citizens to attend meetings in which this project has been designed. Citizens feel that although the condition of Flat Creek has improved in many respects over the years, there are still some perceptions of poor ecological integrity due to water contamination resulting from increasing pressure on the system including population growth, increased urban development, and non-point source pollutants. It is the intention of the Teton Conservation District to assess the overall health and water quality of Flat Creek, and also act as a guard against future negative impacts from occurring on this stream in the future.

This SAP is intended as a guide for personnel who will be conducting the water quality monitoring activities for the Flat Creek Monitoring Project, as a QA/QC plan, and as a data management plan.

Samples are collected using the methods, procedures and/or protocols in the Natural Resources Conservation Service National Handbook of Water Quality Monitoring, May 1998, and the Wyoming Department of Environmental Quality, Water Quality Division, Watershed Program, Manual For Standard Operating Procedures for Sample Collection and Analysis, 1999. A copy of the entire SOP is on file at the Teton Conservation District and all relevant sections of the SOP are contained in the Flat Creek Sampling and Analysis Plan.


Purpose Statement

The Teton Conservation District has responded to public concern of possible

water quality issues affecting Flat Creek by working with landowners along the creek, as well as others interested in the study, to implement a water quality-monitoring project. Specific water quality concerns pertaining to Flat Creek include increased urban impacts such as storm water run-off, septic systems near Flat Creek, garbage and solid waste. There are also concerns of future growth of the town of Jackson and the possible threats this poses to the health of the stream system. Non-point source pollution such as fertilizers and herbicides, as well as the state of the fisheries are concerns as well. Without physical, chemical, and biological data pertaining to Flat Creek, it is difficult to substantiate concerns of these and future human impacts on water quality, habitat, and general health.

There are several purposes to this study. The ultimate purpose for implementing this monitoring project is to establish baseline data where none exists and to establish trends in the water quality of Flat Creek. There were also twelve objectives set forth by the stream committee as guidelines for measuring project success. They are as follows:

1) To improve the condition of Flat Creek, as well as the entire watershed. 2) To collect credible data pertaining to Flat Creek in order to validate concerns. 3) To increase landowner, as well as general public awareness, education, and

participation on issues regarding Flat Creek. 4) To work in conjunction with town and county planners and gain political

strength to take on current problems and prevent negative impacts from occurring in the future.

5) To research ordinances concerning development, define legal jurisdiction against negative impacts, and develop stream laws pertaining to Flat Creek.

6) To assess the number of septic systems along Flat Creek and convert them to the town sewer system.

7) To improve diversions and irrigation management regarding Flat Creek. 8) To assess the amount of private land in the Flat Creek watershed that can be

developed. 9) To collect historical data relevant to Flat Creek. 10) To secure funding for a watershed protection and enhancement program. 11) To improve fisheries habitat where it needs to be done. 12) To use the public school system and the Teton Science School as a resource

for monitoring water quality and also as a means of publicizing efforts to maintain high water quality within the Flat Creek watershed.

Some of these objectives do not deal directly with water quality issues and though

they may not be mentioned in this plan, it is the intention of the Teton Conservation District to meet each of them. Through this study, the Teton Conservation District hopes to better understand the causal effect of human impact on the natural system of Flat


Creek. Our monitoring efforts will answer questions concerned people have been asking for a number of years.

Teton Conservation District employees and field samplers will follow the

guidelines set forth in this sampling and analysis plan, as well as those set forth in the Wyoming Department of Environmental Quality, Water Quality Division, Watershed Program, Manual of Standard Operating Procedures for Sample Collection and Analysis, August 1999.


Sampling Personnel

Organization, Stakeholder, or Group Name

Number Of

Personnel

Type of Training Date of Last

Training

Amount of Previous Sampling

Experience Teton Conservation District 3 NRCS Water Quality

Monitoring Training and WACD Water Quality

Training, Fluvial Geomorphology

Training

2002 6 field seasons

5 projects

Natural Resources Conservation Service

1 NRCS Water Water Quality

Monitoring Training

1999 16 field seasons

150 projects

Private Landowners

Varies Landowners who contribute will be

trained by TCD staff in staff gauge reading, maintaining field log books, and become familiar with field

observation procedures as outlined in the

WDEQ SOP manual.

2000 None


Sampling Design

One of the goals of the Flat Creek water quality-monitoring project is to establish trends depicting the quality of the water of Flat Creek. Trends are considered persistent changes in water quality variables over time. Because of the lack of adequate historical data pertaining to Flat Creek, it will be important to establish a baseline and then monitor the changes, or trends that occur. Trend stations are ideal to monitor changes over time. When establishing trend stations on a water body, it is crucial to use a control station where land use is stable and impact is minimal. There should not be changes in land use practices during the life of the water quality investigation at the control site. The Teton Conservation District has chosen a control site within the Bridger-Teton National Forest boundaries east of the National Elk Refuge. It is an area near the headwaters of Flat Creek that receives little human disturbance with the exception of some recreation that takes place in the vicinity of the control site. Since the US Forest Service manages this area, the land use should not change within the course of the study. The other sites have been chosen to best represent Flat Creek and all possible disturbance areas. The chemical, physical, and biological data collected will be used to draw conclusions as to the overall condition of Flat Creek. The length of the study will depend on the findings, although it is expected to last a minimum of ten years. Following this study, it is the desire of Teton Conservation District to randomly monitor Flat Creek in order to maintain an ongoing understanding of the water body. As trends are established, monitoring schedules may vary depending on ongoing results.

The National Elk Refuge has asked the Teton Conservation District to add two additional sites to the monitoring project to better assess wildlife influences in the area. National Elk Refuge personnel will work closely with the Teton Conservation District in monitoring these two sites which will be located on Nowlin Creek, a tributary of Flat Creek. Their exact locations will be chosen in the spring of 2000 and will be added to this Sampling and Analysis Plan in the Corrective Actions section. A site will also be founded on Cache Creek, another major tributary of Flat Creek that may be directly impacted by urban influence.


Sampling Locations

Sample Site

ID Code

Latitude/Longitude Coordinates from GPS (1/4, section, township, range,

UTM coordinates)

HUC Code for Sample

Site

Type of Land Ownership

Sample Site Location Narrative Flat Creek

FLC 43° 33, 00.00”N 110° 37, 00.00”W

SW ¼ SE ¼ Sec 35 T 42N R 115W

107040103 Public – USFS (site determined by committee)

Control: Flat Creek on Bridger-Teton National Forest. Follow Elk Refuge road from East Jackson to Forest Service bndry at Flat Creek USFS sign make right down

steep road to site

FL1 43° 33, 27.00”N 110° 43,75.00”W

NW ¼ NE ¼ Sec 11 T 41N R 116W

107040103 Public – FWS (site determined

by TCD and USFW-NER)

Park at National Fish Hatchery. Walk out road to NE on Elk Refuge. After 200 yards head down to Flat Creek

and look for Stake along west bank of Creek.

FL2 43° 29, 00.00”N 110° 46, 00.00”W SE ¼ NE ¼ Sec 28

N 41N R 116W

107040103 Private (site determined by committee)

Just below Saddle Butte Bridge on Flat Creek

FL3 43° 29’ 02.00”N 110° 46’ 00.00”W

NW ¼ NE ¼ Sec33 T 41N R 116W


In Jackson, drive to the end of Hansen Ave. at Flat Creek. Walk

down to creek and site is below Cache Creek outlet

FL4 43° 27’ 30.00”N 110° 48’ 00.00”W SE ¼ NE ¼ Sec 06

T 41N R 116W


In Jackson, just north of High School Road Bridge on Flat Creek.

FL5 43° 23’ 00.00”N 110° 46’ 00.00”W

NW ¼ SW ¼ Sec 28 T 40N R 116W

107040103 Public-WY Game & Fish

(site determined by committee)

Drive on South Park Elk Feed Gounds access road from Highway 89 west to

bridge over Flat Creek. Site is 50 yards upstream.

NC1 43° 31’ 16.00”N 110° 42’ 93.00”W

SE ¼ NW ¼ Sec 13 T 14N R 116W

107040103

Public – NER Management

(site determined by TCD and USFW-NER)

From East Jackson, drive onto Elk Refuge road. Take left to Nowlin Creek road on north side of Miller

Butte. After third pond walk down to creek. Site is 50 yards past pond

outlet. CC1 43° 27’ 8.91”N

110° 42’ 11.43”W 107040103

Public-USFS Drive to Cache Creek Trailhead and walk 1.8 miles up forest road. Walk down to Cache Creek and look for

green USGS gauging staion. CC2 43° 27’ 55.84”N

110° 43’ 52.72”W

107040103 Public-USFS Drive to Cache Creek trailhead. Walk down the creek until reaching foot bridge. Sample spot is just below

bridge.


CC3 43° 29’ 02.00”N 110° 46’ 00.00”W

NW ¼ NE ¼ Sec33 T 41N R 116W

107040103 Private In Jackson, drive to the end of Hansen Ave. at Flat Creek. Walk down to

creek and site is at Cache Creek outlet where large concrete culvert exits

ground. GC1 43° 25’ 23.90”N

110° 42’ 6.19”W

107040103 Public-USFS Park at Game Creek trailhead and walk 3.3 miles up Forest Service Road to a crossing of the north fork Game

Creek. Take sample just above crossing.

GC2 43° 23’ 39.14”N 110° 43’ 15.54”W

107040103 Public-USFS Park at the Game Creek trailhead and walk 1/3 of a mile to the first crossing

of Game Creek. The sample site is just above the culvert.

GC3 43° 23’ 37.34”N 110° 43’ 42.07”W

107040103 Public-USFS Park at the Game Creek trailhead. Walk below Game Creek Road where creek exits culvert. Take sample 30

feet below culvert. GC4 43° 23’ 34.68”N

110° 44’ 0.89”W 107040103 Public - USFS Drive 0.8 miles up Game Creek Road

from Highway 89. Walk directly south to Game Creek.


Proposed Sampling Schedule (indicate whether it is periodic sampling or event driven sampling) Sampling Category

(all sites receive same sampling)

Proposed Sampling Dates for Periodic Sampling

Event(s) Parameter(s) to be sampled

Biological

Calendar yearly sampling, of macros and fecal coliform

beginning in late Fall of 2000 and continuing for the next 5-10 years, dependent upon ongoing

results. Three sites will be selected for electro-shocking in September by Wyoming Game and Fish and work will be done two days per year in September.

Macroinvertebrates, Electro-shocking, Redd counts, Fecal Coliform

sampling

Physical One time annually in late Fall, dependent upon stream flow

level.

Discharge, Embeddedness, Habitat Assessment, Pool/riffle

ratio, Substrate

Chemical

Specific chemical parameters including pH, DO, Temperature, and Conductivity measured during physical sampling which will take place annually in late fall.

Full-scale chemical sampling will take place twice each year, dependent upon pre-runoff and post-runoff times.

Chloride, Fluoride, Nitrate, pH, Total dissolved solids, Total Nitrogen, Total Phosphorous, Total Suspended Solids, Turbidity


Parameters and Sample Collection Methods

Parameter Reporting Units

Test Method Number or Name

Preservative Holding Time

Chemical Parameters

Biochemical Oxygen Demand (BOD)

mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 405.1 Ice

48 Hours

Dissolved Oxygen (DO)

mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 360.2, USEPA 1983

Probe: None Must be measured on site

Chloride mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 325.2

None required

28 Days

Fluoride mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 340.2

None required

28 Days

Phosphorous, Total mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 365.3, colorimetric

Sulfuric Acid 28 Days

pH Units USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 150.1

None Must be measured on site


Parameter Reporting

Units Test Method Number or

Name Preservative Holding Time

Temperature N/A

USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 170.1

None Must be measured on site

Total Dissolved Solids (TDS)

mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 160.1

Ice 7 Days

Total Nitrogen (Kjeldahl)

mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, 351.3

Sulfuric Acid 28 Days

Total Suspended Solids (TSS)

mg/l USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 160.2

Ice 7 Days

Turbidity NTU USEPA Methods for Chemical Analysis of Water and Wastes, EPA-600 / 4-79-020, Method 180.1

None Must be measured in field


Biological Parameters




e. coli bacteria with result reported as CFU’s (colony forming units) per 100 ml

CFU’s SOP for e. coli bacteria sampling, DEQ-WQD SOP Manual, August 1999 adapted from USEPA, Microbiological Methods for Monitoring the Environment, Water and Wastes, EPA-600 / 8-78-017, December 1978, p.124-130, p.59-90, incorporated by ref. Office filtration and incubation, HACH EPA Approved Method 10029, Coliforms: Membrane Filtration (simultaneous detection), also see Teton Conservation Distric’s Sampling and Analysis Plan for Bacterial Density Analysis, January 2003

Ice immediately. Samples must be kept at or below 4° C.

6 Hours

Macroinvertebrates N/A SOP for Macroinvertebrate sampling and for macroinvertebrate sample preservative, DEQ-WQD SOP Manual, August 1999

Formalin; see SOP

Once sample is preserved, keep out of direct sun and away from direct exposure to heat.

Electro-shocking and redd counts (contracted out to WY Game & Fish)

N/A SOP for electro-shocking and redd counts as guided by Wyoming Game & Fish. Contact John Keifling at Wyoming Game and Fish (Jackson Branch) for further information.

None Must be measured in field.


Physical Parameters




Bank Stability N/A Refer to SOP Manual, WDEQ, Water Quality Division

N/A Must be measured in the field.

Bankfull Width N/A Refer to SOP Manual, WDEQ, Water Quality Division


Riffle Gradient N/A Refer to SOP Manual, WDEQ, Water Quality Division


BURP Monitoring Strahler Stream Order Method

N/A Refer to SOP Manual, WDEQ, Water Quality Division


Stream Discharge N/A Refer to SOP Manual, WDEQ, Water Quality Division


Embeddedness N/A Refer to SOP Manual, WDEQ, Water Quality Division


Global Positioning System Data (GPS)



Habitat Assessment High Gradient Streams



Habitat Assessment Low Gradient Streams



Pool Quality N/A Refer to SOP Manual, WDEQ, Water Quality Division


Width to Depth Ratio




Health and Safety The Teton Conservation District will take the maximum precautions regarding health and safety into the Flat Creek monitoring project. Field samplers will have first aid kits with them at all times and will be trained in basic first aid and adult CPR. All field samplers will have knowledge of the surrounding hospitals and emergency medical clinic locations. Field samplers will always sample with at least two personnel to assure safety. Sampling personnel will always follow health and safety measures outlined in the DEQ-SOP, August 1999.

EMERGENCY PHONE NUMBERS AND ADDRESSES:

ST. JOHNS HOSPITAL EMERG+A+CARE OF JACKSON HOLE EMERGENCY – DIAL 911 EMERGENCY – DIAL 911 OR CALL (307) 733-3636 OR CALL (307) 733-8002 625 EAST BROADWAY POWDERHORN MALL 974 WEST BROADWAY JACKSON, WY 83001 JACKSON, WY 83001 TRAINING FOR FIRST AID AND CPR ST.JOHN’S HOSPITAL CALL 733-3636 FOR CLASS INFORMATION OR TETON COUNTY PARKS AND RECREATION 733-5056


Corrective Actions

Any need for modification in the existing monitoring plan including; site

locations, number of samples per site, number or type of quality control samples, sampling methods or standard operating procedures, number of sites, or database application will be decided concurrently by the project manager, the stream committee, and field samplers. If any change is warranted, the project manager will insert the dated amendment in to the Sampling and Analysis Plan and send the amendment to the Department of Environmental Quality to insert in their copy. The project manager will also notify all field samplers of amendments in the SAP. The project manager on an as needed basis will assess the effects of changes in the monitoring plan incurred. In the event that any corrective action needs to be implemented, Teton Conservation District employees and field samplers will follow procedures outlined in the WDEQ – SOP Manual, August 1999.


Calibration and Maintenance of Equipment

Item Log(s) Calibration Maintenance SOP

Hach sensION156© MultiProbe pH, DO and Conductivity Meter

Calibration and Maintenance log kept on file at the Teton Conservation District.

Once a day. pH 7 and 10 buffer standards are supplied by the conservation district; DO probe is air calib.

No field maintenance except electrode replacement; meters are returned to the conservation district for repair

Collect water samples which best represent the entire water body. For complete detail, refer to WDEQ SOP Manual.

Surber© Surber Sampler

Maintenance log kept on file at the Teton Conservation District.

None required Check net before each use for tears and holes in the netting; remove any leftover debris

Stand downstream from site and collect macroinvertebrate sample. For complete detail, refer to WDEQ SOP manual.

Magellan© GPS ColorTRAK Satellite Navigator GPS (Global Positioning System)

Calibration log kept on file at the Teton Conservation District.

Time set as needed (system does not account for daylight savings changes)

No field maintenance; GPS is returned to the conservation district and sent to the manufacturer for repair

Use system to calculate specific location. For complete detail, refer to WDEQ SOP manual

Ben Meadows DH-48 Integrated water sampler

Maintenance log kept on file at the Teton Conservation District.

No calibration necessary.

No field maintenance; DH-48 is returned to the conservation district and sent to the manufacturer for repair.

Use system to integrate water samples. For complete detail, refer to WDEQ SOP manual.

Marsh-Mcbirney FLO-MATE Model 2000 w/wading rod

Calibration and Maintenance log kept on file at the Teton Conservation District.

No calibration necessary.

No field maintenance; FLO-MATE is returned to the conservation district and sent to the manufacturer for repair.

Use system to measure flow and velocity in streams and rivers. For complete detail, refer to WDEQ SOP manual.


Quality Control Samples

Sample Site ID Code

Type of QC Sample

Number or % of QC Samples

QC Sample Schedule

FLC Duplicates and Field Blanks

1 duplicate every five sites, and one field blank per sampling event

Duplicate sample is done every fifth site Field blank is randomly chosen by project manager, who notifies field sampler.

FL1 Duplicates and Field Blanks










1 duplicate every fifth sites, and one field blank per sampling event





NC1 Duplicates and Field Blanks



CC1, CC2, CC3

Duplicates and Field Blanks



GC1, GC2, GC3, GC4

Duplicates and Field Blanks




Sample Testing/ Contract Labs Laboratory

Address Contact Tests

Aquatic Biology Associates 3490 NW Deer Run Road Corvallis, OR 97330

Bob Wisseman Senior Scientist (541) 752-1568

Macroinvertebrate Sample Count and Identification

Department of Health and Welfare Laboratory

465 Memorial Drive Pocatello, ID 83201

Ardith Moran Branch Lab Supervisor (208)233-4341

Fecal Coliform and E. Coli Identification

Wyoming Department of Agriculture Analytical Services

1174 Snowy Range Road Laramie, WY 82070

Ken McMillian Senior Scientist (307)742-2984

Surface Water Collection and Analysis

Wyoming Game and Fish P.O. Box 67 Jackson, WY 83001

Ralph Hudelson Fisheries Biologist (307) 733-2321

Electro-shocking and redd counts

Macroinvertebrate and surface water samples will be delivered by way of courier. Surface water samples for chemical analysis must arrive at the lab to be analyzed within 48 hours. The samplers must mail surface water samples on the same day they are collected to ensure their timely arrival. Macroinvertebrate samples must be kept in an alcohol preservative (see WDEQ SOP 1999) and out of direct heat and sunlight until they are shipped to the lab.

Macroinvertebrate samples will be sent via United Parcel Service (UPS) and surface water samples will be sent via Federal Express. It is the samplers’ responsibility to pack the water samples and take them to Federal Express before 5:00PM Monday through Thursday to be shipped to the lab. Samplers should avoid sampling for surface water on Friday, as samples will not be analyzed within 48 hours due to the weekend. Fecal Coliform and E. Coli counts have a holding time of only six hours and therefore need to be delivered to the laboratory in Pocatello, Idaho on the same day as sampling takes place. Samplers should arrive at the lab by 2:30PM as instructed by the lab. Samplers will need to start sampling early enough to accommodate the arrival of samples to the lab by the appropriate time.

Electro-shocking and redd counts will be performed by Wyoming Game and Fish and the Teton Conservation District will assist in the sampling efforts each September. Wyoming Game and Fish will follow proper procedure as detailed in Wyoming Game and Fish sampling protocol. Sampling results will be given to the Teton Conservation District in the form or paper or electronic results and incorporated into the Flat Creek Monitoring Project files.


Field Log Books

When using field log books, samplers will follow standard operating procedures outlined for field log books as detailed in the WDEQ – SOP Manual August, 1999.

The front cover will appear as follows:

TETON CONSERVATION DISTRICT FIELD LOG BOOK

SAMPLERS NAME (PRINTED): DATE FROM: mm/dd/yy - / / DATE TO: mm/dd/yy - / / SEQUENTIAL LOG BOOK NUMBER: The inside cover will appear as follows:


(ANY PERSON WHO MAKES AN ENTRY IN THIS LOG BOOK MUST SIGN HERE) SAMPLER SIGNATURE DATE INITIALS


Field Log Books

The entry pages will appear as follows:


OBSERVER: DATE AND TIME: DISCHARGE RATING: CURRENT WEATHER CONDITIONS: WEATHER CONDITIONS (IN PREVIOUS 12 HOURS): RECENT PRECIPITATION (IF SO, HOW MUCH?: STAFF GUAGE READING: FIELD OBSERVATIONS:


Data Archiving

Data Item

Location Retention Time

Chain of Custody forms Teton Conservation District office

3 Years

Equipment Calibration Logs Teton Conservation District office

3 Years

Field Data Sheets Teton Conservation District office

Permanently

Field Log Books Teton Conservation District office

Permanently

Laboratory Test Results (original raw data)

Teton Conservation District office

Permanently

Database/Spreadsheets Teton Conservation District office

Permanently

Statistical Analyses Teton Conservation District office

Permanently

Final Report Teton Conservation District office

Permanently

Photographs Teton Conservation District office

Permanently

When data archiving, Teton Conservation District employees and field samplers will always follow procedures outlined in WDEQ – SOP Manual, August 1999.


Data Review and Validation

All data collected and reported will receive a 100 percent error check. The monitoring supervisor and the Natural Resources Conservation Service District Conservationist will be responsible for all data review and validation. Both will use methods and correction procedures as outlined in the USDA-NRCS National Handbook of Water Quality Monitoring for accuracy in all review and validation.

Laboratory Results

All laboratory results will be stored in paper and electronic format. Direct results sent from laboratories will be kept in a file at the Teton Conservation District. Results will also be entered into electronic database format for analyses. A copy of all electronic database entries will be kept in the form of zip disc and stored off of Teton Conservation District property in case damage to property occurs. Paper report will be put into electronic file by the project supervisor, or will be delegated to other staff and supervised by the project supervisor. Laboratory results will be analyzed and used to evaluate the condition of Flat Creek and program effectiveness.

Photographic Documentation

Photographic documentation will start with the BURP/BIOASSESSMENT field data sheet in which the field sampler will fill in the appropriate blanks pertaining to taking photographs. Field sampler will indicate the frame number, date, and time of the upstream, downstream, and panorama photographs taken. Field samplers will take one photograph upstream and one downstream. Panorama photos will consist of one frame taken in all directions including North, Northeast, East, Southeast, South, Southwest, West, and Northwest, which will account for a 360° view of the site. All captions should include frame number, date and time, subject, location in narrative, and latitude/ longitude coordinates (which are included on the first page of the field data sheets. At the end of each sampling event or day, all photographs taken will be downloaded into the computer at the Teton Conservation District and cataloged. A copy of all photographs taken will be kept on zip disc off Teton Conservation District property in case damage to conservation district property occurs.

The Teton Conservation District uses a FUJIFILM MX-700 digital camera. The camera is kept at the Teton Conservation District and instructions are kept with the camera in its case. Field samplers will always follow standard operating procedures for photographic documentation as outlined in the WDEQ – SOP Manual, August 1999.


Landowners and Contact Information

The Flat Creek Monitoring Project is a collaborative effort between the Teton Conservation District and local landowners that have expressed interest in the project. The control site (FLC) is located on Bridger-Teton National Forest land. Sites two through four, (FL2-FL4) are located on private property. Site five (FL5) is located on the Wyoming Game and Fish Big Game Refuge. Sites seven (NC1) is located on Nowlin Creek on the National Elk Refuge. Cache Creek sites one and two (CC1 and CC2) are located on Bridger-Teton National Forest and site three (CC3) is located on private property. All Game Creek sites are located on Bridger-Teton National Forest. The Teton Conservation District has the permission from the US Forest Service, the National Elk Refuge, Wyoming Game and Fish, the National Fish Hatchery, and all other landowners to monitor as necessary on their property and will always notify landowners at least one day in advance of monitoring efforts. The following list represents landowners and contact information regarding the monitoring sites:

Contact Site Telephone Number Address US Forest Service Contact – Nancy Hall

FLC (307) 739-5400 P.O. Box 1888 Jackson, WY 83001

National Fish Hatchery Contact – Kerry Grande

FL1 (307) 733-2510 1500 Fish Hatchery Jackson, WY 83001

Triano and Copp FL2 (307) 733-0871 P.O. Box 20108 Jackson, WY 83001

Pete Karns

FL3 (307) 733-6060 P.O. Box 40 Jackson, WY 83001

Mike Martin FL4 (307) 733-9481 P.O. Box 1743 Jackson, WY 83001

Wyoming Game and Fish Contact – Steve Kilpatrick

FL5 (307) 733-6333 P.O. Box 67 Jackson, WY 83001

National Elk Refuge Contact – Eric Cole

NC1 (307) 733-9212 P.O. Box C Jackson, WY 83001

US Forest Service Contact – Nancy Hall

CC1, CC2, GC1, GC2, GC3 and GC4

(307) 739-5400 P.O. Box 1888 Jackson, WY 83001

Pete Karns

CC3 (307) 733-6060 P.O. Box 40 Jackson, WY 83001

.


Report Generation

All paper reports will be kept in the Flat Creek Monitoring project files at the Teton Conservation District. Paper reports including all reports sent to the conservation district by contract labs will be kept on file at the district office. Information collected will be stored in electronic format as well and reports generated from information collected will be in electronic form. The district will produce reports on the findings of the monitoring project and will use GIS for the generation of these reports. Field samplers will always comply with procedures outlined in WDEQ – SOP Manual, August 1999 when generating reports.

Statistical Analyses

The Teton Conservation District has taken measures to ensure that the number of sample sites is adequate as well as ensuring that the correct number of samples are being taken in order to reduce the amount of statistical variation. The district has also taken adequate quality control measures to assure that the findings of this project are accurate and best represent the water quality of Flat Creek.

The format for analyses is yet to be determined by the Teton Conservation District, which is awaiting Phase V training of Water Quality Monitoring to be sponsored by The Department of Environmental Quality, Wyoming Department of Agriculture, and the Wyoming Association of Conservation Districts. Upon completion of this training, the Teton Conservation District will develop a database management system that complies with policies set forth within the Phase V training session.

Manual of Standard Operating Procedures for Sample Collection and Analysis

TABLE OF CONTENTS (NOTE: This table of contents only contains subjects relative to the Teton Conservation District’s Flat Creek Monitoring Project. The

entire SOP is on file at The Teton Conservation District.) PART 1 – SAMPLE PLANNING AND SITE LOCATIONS Below Only Monitoring……………………………………………………………….1 – 2 BURP Monitoring Field Data Sheet Review………………………………………….1 – 2 BURP Monitoring Preliminary Data Review…………………………………………1 – 2 BURP Monitoring Procedure Sequence……………………………………………….1 –2 BURP Pre-Monitoring Site Evaluation………………………………………………..1 – 2 BURP Reference Site Locations (including bioassessment reference sites)……….....1 – 2 BURP Reference Site Scheduled Monitoring…………………………………………1 – 2 Non-point Source Pollution Bioassessments…………………………………………..1 –2 Sampling Locations, Determining……………………………………………………..1 –2 PART 2 – BIOLOGICAL SAMPLING Fecal Coliform Sampling………………………………………………………………1 - 4 Fecal Coliform Testing (including Bacterial Sample Analysis Log)………………….1 – 8 Geometric Mean, Calculating and Using (for fecal coliform test results)……………..1 –4 Macroinvertebrate Sample Identification (including Subsampling Form)…………….1 –8 Macroinvertebrate Sample Preservative…………………………………………...…..1 –2 Macroinvertebrate Sampling…………………………………………………………..1 – 6


PART 3 – PHYSICAL SAMPLING Bank Stability, Evaluating…………….………………………………………………1 – 2 Bankfull Width, Determining…………………………………………………………1 – 2 Bioassessment Riffle Gradient……………………………………………………...…1 – 2 BURP Monitoring Strahler Stream Order Method…………………………………....1 – 2 Current Velocity, Measuring for BURP Monitoring………………………………….1 – 2 Discharge, Stream………………………………………………………………….….1 – 4 Embeddedness (silt covering) Rating…………………………………………………1 – 2 Geology Abbreviations, Surficial……………………………………………………..1 – 6 Global Positioning System (GPS) Data (including GPS Locational Data Sheet)…….1 – 4 Habitat Assessment, High Gradient Streams………………………………………….1 – 4 Habitat Assessment, Low Gradient Streams…………………………………………..1 – 4 Per Cent Fines…………………………………………………………………………1 – 2 Pool Quality…………………………………………………………………………...1 – 2 Soil Type Abbreviations and Definitions……………………………………………..1 – 4 Width to Depth Ratio………………………………………………………………….1 – 2 PART 4 – CHEMICAL SAMPLING Biochemical Oxygen Demand (BOD), 5 day 20• C…………………………………..1 – 2 Chloride………………………………………………………………………………..1 – 2 Dissolved Oxygen (DO)………………………………………………………………1 – 2 Fluoride………………………………………………………………………………..1 – 2


Hardness, Total (as Calcium Carbonate)……………………………………………...1 – 2 Nitrogen, Total Kjeldahl………………………………………………………………1 – 2 pH……………………………………………………………………………………...1 – 2 Temperature, Water…………………………………………………………………...1 – 2 Total Dissolved Solids (TDS)…………………………………………………………1 – 2 Total Suspended Solids (TSS)………………………………………………………...1 – 2 Turbidity………………………………………………………………………………1 – 2 PART 5 – QUALITY CONTROL, CUSTODY AND REPORTING Abbreviations………………………………………………………………………….1 – 4 Bioassessment Quality Control Criteria……………………………………………….1 – 2 Blanks………………………………………………………………………………....1 – 2 BURP Monitoring Core Parameters…………………………………………………..1 – 2 BURP Monitoring Electronic Data Entry……………………………………………..1 – 2 Chain of Custody (including three types of Chain of Custody Forms)……………….1 – 8 Cleaning Previously Used Sampling Bottles………………………………………….1 – 2 Conversion Factors……………………………………………………………………1 – 6 Data Archiving (including sample Transfer of Records and Box Label Forms)……...1 – 4 Duplicates……………………………………………………………………………..1 – 2 Ecoregion Definition…………………………………………………………………..1 – 2 Field Log Books……………………………………………………………………….1 – 2 Holding Time………………………………………………………………………….1 – 2 Instrument Calibration and Calibration Logs…………………………………………1 – 2 Macroinvertebrate Reference Collection……………………………………………...1 – 2


Material Safety Data Sheets (MSDS)…………………………………………………1 – 2 Photographic Documentation…………………………………………………………1 – 8 Quality Control Measures, Summary of……………………………………………....1 – 4 Safety and Safety Equipment…………………………………………………………1 – 2 Sample Collection……………………………………………………………………..1 – 2 Sample Labeling………………………………………………………………………1 – 2 Sample Preservation…………………………………………………………………..1 – 4 Split Samples………………………………………………………………………….1 – 2 Surber Sample Techniques……………………………………………………………1 – 2



APPENDIX A

• CREDIBLE DATA LEGISLATION


APPENDIX B

• FORMS

1. DEQ-WQD Watershed Program BURP Monitoring Field Data Sheets 2. Chain of Custody Form 3. Equipment Calibration and Maintenance Log (example)


APPENDIX C

• Sample Labeling (example)


APPENDIX D

• Sampling Site Location Maps


APPENDIX E

• References and Information Resources


APPENDIX F

• Standard Operating Procedures o Table Of Contents Included

Documents

TETON CONSERVATION DISTRICT Conservation District employees and field samplers will follow the guidelines set forth in this sampling and analysis plan, as well as those set forth in