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Home / Library / Collections / Sciences / Biology / Fundamentals of Biology /
Chapter 01: Them es of Bio logy
Chapter 01: Themes of Biology
Biology is defined simply as the study of life. It is a very broad term that encompasses a wide
array of much more specific studies. There are marine biologists, microbiologists, zoologists,
ecologists, and dozens of other types of biologists. Even with so many different areas of
study, all biologists need to understand the basics, which is the fundamental purpose of this
book.
Key Topics: Biological Order, Cell Theory, Properties of Life, Evolution & Natural Selection,
Studying Biology
Biological Order
All the different types of biologists study very different types of living things. Some work on a
microscopic scale and look at the individual components of living organisms, while others look at
the broader picture, and focus on how different types of organisms interact with one another.
These different levels of life can be categorized into a Biological Order. This scale starts very
large, and encompasses all the life on the planet, but can be broken down to something as tiny as
INTRODUCTION
the study of a single atom. The entire scale is
depicted in the diagram.
The theory of biological order is a simple one,
start from the outside and work your way in.
Because this is an introductory series, we will
only extensively cover information about Cells,
Organelles, Molecules, and Atoms – the four
lowest levels of Biological Order on which all
the rest of life is based. These topics will
provide the foundation for your continued
education in Biology.
It is also important to remember that biological
systems are far more complicated than their
single components. Just because you
understand how an atom works does not
mean you understand how a molecule works.
As you progress through this text, you should constantly be questioning how different things will
interact with one another, and what could happen if one of the components in a system fails.
Cell Theory & History
The Cell Theory states that cells are the basic fundamental units of life, and that cells originate
from other cells.
The first recorded observation of cells occurred in 1665, when Robert Hooke, an early English
scientist, looked at a thin cork sample placed under a basic magnifying lens. What he saw was
the thick cell walls between the cork cells. He illustrated his findings and is credited as being the
first to discover cells.
The next major milestone in cell observation occurred in the late
17 and early 18 centuries, when Anton van Leeuwenhoek
began building his own microscopes. He achieved an
impressive magnification of 300x, and was the first to use
microscopes to view living cells. His other major
accomplishments include being the first to observe bacteria,
cell vacuoles, and red blood cells. Although his discoveries
were well documented, he did not fully document how he went
about making his microscopes. This slowed major progress on the study of cells for nearly a
century, as microscopes were not readily available to scientists.
Theodor Schwann and Matthias Schleiden were scientists in Germany during the early 1800s.
Through collaboration, they established the first portion of the cell theory, stating that all living
things are made up of cells. Another German scientist, Rudolf Virchow, helped revise the theory to
include a statement about cell reproduction. His portion of the theory stated that cells come from
preexisting cells.
General Cell Properties & Types
In addition to the two basic components of the cell theory, it is also important to note that all cells
are enclosed by a cell membrane, and that all cells contain DNA (genetic material) at some point
in their lifespan. Cells will be discussed in greater detail later.
It is also important to know that cells are classified into two general categories, Prokaryotic and
Eukaryotic. They each are very different and are easily distinguishable from one another.
Prokaryotic cells (pictured in the diagram to the left) are small compared to Eukaryotic cells,
are missing many of the organelles found in Eukaryotic cells, and they do not have a nucleus.
Prokaryotic cells have existed on Earth much longer than Eukaryotic cells and therefore are much
more simple and primitive. In a sense, Eukaryotic cells can be viewed as an evolution of
Prokaryotic cells.
th th
Unlike Prokaryotic cells, Eukaryotic cells contain many different organelles ("tiny" organs), each
with specialized functions. Additionally, their organelles follow the rules of
compartmentalization, meaning that their organelles are arranged into specific areas of the
cell. They also contain DNA, their genetic material, within their nucleus. You can easily tell that the
right cell in the diagram below is Eukaryotic because of its many complex internal structures.
The Properties of Life
How do you prove whether or not something is living? What traits are universally common
across all different forms of life? These types of questions led the scientific community to
develop a list of seven Properties of Life, which are characteristics that all living organisms
exhibit. An overview of these properties is shown below.
Cell Theory: All Living Things are made up of Cells
Biological Order: Life follows Biological Order, in that all the characteristics of an organism
are the result of the many cells, tissues, and organs making it up.
Homeostasis: All living things must be able to regulate their internal environment (body
temperature, etc.)
Metabolism: All organisms must be able to perform metabolic reactions, which allow
organisms to obtain and use energy.
Development: All living organisms must Grow, Develope and Reproduce.
Response: Organisms must be able to Respond to Changing Conditions in the Environment,
like birds migrating in winter.
Evolutionary Adaptation: All organisms must Adapt to the Environment over time, through
evolution.
Form Follows Function: The shapes of biological structures (cells, organelles, organs,
etc.) are correlated with the individual function of the structure. Flowers aren’t beautiful just
for the sake of being beautiful. Every stem, leaf, and petal serves a function.
Evolution & Natural Selection
Evolution is an accepted scientific theory stating that different kinds of organisms have
developed from earlier, often simpler, forms of life in order to adapt to changes in their
environment. Evolution occurs as the result of Natural Selection. Natural Selection is the idea
that a small number of organisms within a population will be born with a random genetic
modification. If this genetic modification proves useful to the survival of the organism, then the
organism is more likely to reproduce, and its mutated trait is more likely to pass on. Likewise, if
the organism is given a gene that proves to be a hindrance to its survival, then the organism is
more likely to die before reaching reproductive age, and will not pass on the unwanted gene.
A good, but sad example of observable natural selection has occurred over the past few decades
in Africa amongst Elephants, who are ferociously pursued by poachers for their ivory tusks. At
random, a genetic mutation has allowed some elephants to be born without tusks. Traditionally,
this would prove to be a hindrance on their survival, because elephants need tusks to ward off
attackers. Now, in some areas, it has almost become a necessity for survival, because poachers
will not waste their time killing a tuskless elephant.
Although some examples of natural selection can be visibly seen occurring, most are not. Most
evolutionary adaptations result after hundreds of thousands, if not millions of years of natural
selection. Birds did not evolve their bones to be light and somewhat hollow over the course of just
a couple of years to enable them to fly. It took millions of years, and countless amounts of genetic
modifications to give them the unique traits they have today.
Studying Biology With Experiments
Biologists, like all other scientists, have to follow a certain set of rules and proceedures as they
progress through experiments. This set of rules is known as the Scientific Method. It contains
five steps. It is especially important that you know and understand all the steps of the scientific
method, because it is highly likely that you will have to use it if you ever attend a labratory class (in
any science field).
The fist step to the scientific method is to Ask a Question about something unknown based on
observations you are experiencing, or have experienced. This is also known as making an inquiry
about the unknown. This step is essential, because asking questions and then attempting to
answer those questions is the basis of any type of scientific study.
Once a question has been formed, scientists move on to produce a hypothesis. A hypothesis is
a possible solution to the question being asked. Hypotheses are based on other relevant scientific
findings, and should make logical sense. It is also important that the hypothesis is testable. In
other words, there must be some way to prove the hypothesis to be true or false.
After a proper hypothesis has been formed, the scientist can go about performing a controlled
experiment. Controlled experiments are methodical tests designed to answer a hypothesis. At
their most simplest level, controlled experiments contain two types of variables. Variables are
anything that can change based on things that the experimenter does, and can affect the outcome
of an experiment. Variables can be almost anything. Temperature, acid levels, moisture, and
lighting amounts are just some examples of variables that can be manipulated in experiments.
Only one variable should be manipulated at a time during an experiment. This variable is known as
the maniuplated variable. For example, if you wanted to test the effects of adding acid to a fish
embryo you would manipulate the acidity levels. You would not change the temperature of the
embryos, the amount of sunlight the embryos are typically exposed to, or the hardness of the
water. You would only change the acidity levels. All the variables that do not change are referred to
as constants , because they must remain constant, or the same throughout the entire
experiment.
Controlled experiments should contain two
different types of groups – a control group
and a variable group. This helps to ensure
the validity, reliability, and accuracy of an
experiment. The subjects being tested are
divided into one of these two groups at
random. The control group is the group
that remains the same throughout the entire
experiment – they are not affected by
whatever variable is being manipulated. The
variable group is the group where the
manipulated variable is modified. Returning
to the example of acidity levels in fish
embryos, one group of embryos would be
left alone, and a second group would
receive the acid treatment. The constants
would still remain the same between the two groups. Having a control group is very important,
because it gives the experimenter something to compare the effects of the manipulated variable
to. For example, if changing acidity levels in the variable group of fish embryos to a higher
concentration results in the embryos dying, and the control group of embryos are still alive, then
the scientist can safely conclude that the acid is what resulted in the embryo deaths. If however,
the scientist raises the acidity levels in the variable group, and then both the control group and the
variable group of embryos die, the scientist cannot conclude that it was the acidity levels that
resulted in the deaths. In this case, one of the constant controlled variables resulted in the deaths
of the embryos. The experimenter would have to figure out what caused the embryos to die, and
then they would have to perform the experiment over again with new, corrected control variables
that ensure that the control group does not die during the duration of the experiment.
It is also possible for the scientist to test more than one level of acidity in the experiment at the
same time. If a scientist wanted to determine what levels of acidity resulted in embryo deaths,
they would use one control group, and multiple variable groups at varying acidity levels. Note that
the manipulated variable – the acidity- remains the same. No other variables are changed. The
scientist could then observe the results. They might find in the end that the control group, and the
variable group with the lowest amount of acidity stay alive, and that the remaining groups with
higher acidity levels die. The scientist would record this information as data. Data is the
information that is collected from experiments. In this case, the scientist would collect two types of
data – the acidity levels, and the state of the embryos. It might look something like the table below.
Scroll horizontally to view the whole table
GroupControl
(7.0)
6.5 (Low
Acid) 5 (Med Acid) 3 (High Acid)
Embryo
StateAlive Alive Dead Dead
The scientist would likely use more incrementing levels of acidity than in the example above, but
you get the idea. As acidity increases the embryos begin to die.
Once the scientist finished performing the experiment, they analyze the data and form a
conclusion. In the embryo example, the scientist would look at the data and determine that the
embryos will die when exposed to highly acidic environments. This allows them to form
a conclusion about their experiment, which states whether or not their original hypothesis was
correct or not. If the hypothesis stated that “rising acidity levels in fish embryos has no effect on
their health” them the experimenter’s hypothesis would have been proven untrue by the
experiment. If, on the other hand, the scientist stated in their hypothesis that “high acidity levels in
fish environments results in embrio deaths,” then their hypothesis would have been correct.
Once the scientist has drawn their conclusion, they share their results with the rest of the
scientific community. Generally, this is done through scientific writing and experimental reports,
which are then published online in scientific journals.
Experimental Ethics & Forbidden Experiments
Although seeking out and understanding
knowledge is important to the evolution
of humanity, there are certain prices that
can never be justifiably paid in order to
obtain knowledge. For example, during
the Holocaust the Nazis killed millions of
Jews, but this is not all they did. Some
Nazi scientists were performing
inhumane experiments on the prisoners.
This gave them vital information which led to the advancements of some modern medical
sciences that people benefit from today. Even so, these advancements are the end result of a
horrifically tragic violation of human rights. Modern ethics and rules have been established to
ensure the safety of people who choose to partake in experiments.
When planning experiments, keep the following in mind:
If you are using human subjects, they must be consenting. If any risks are involved with the
experiment, they must be clearly stated. Written consent should be documented.
Human subjects must be voluntary. People must not be threatened or bribed to volunteer.
The names of your participants must remain confidential. They should be referred to as
"Subject A" and such when mentioned in research papers.
If you are using animal subjects, you must comply with very strict animal testing guidelines.
Scientific Writing
As a biology student, it is important to be able to write, analyze, and understand scientific writing.
Scientific writing is used to convey the results of experiments. Virtually every research article in
biology journals will follow a specific format, known as the IMRAD format. The term IMRAD is an
acronym, and stands for “Introduction, Methods, Results and Discussion.” Although these four
sections make up the general overview of how a scientific paper should look, there are, in total,
about nine portions to every piece of scientific writing.
Title Page
The title page is a separate sheet of paper at the beginning of a report that generally
contains the title of the experiment, the name of the experimenters, and the date the
experiment was performed.
Abstract
Abstracts are summaries of the report. They outline the rest of the lab paper, and are used
so readers can decide whether or not the report is relevant to their studies. It generally
makes sense to write the abstract after the rest of the report has been written.
Introduction
The introduction gives the reader enough information so they can better understand why the
experiment is being performed, what the experiment is building upon, and how the
experiment works.
Methods
The methods section contains all the information about the materials and tools needed to
set up the exam, the amounts of substances used, and the proceedures that went into
performing the experiment. It should be written in a way that enables other experimenters to
perform the experiment and confirm its results.
Results
The results section of the report is where the findings of the report can be found. The
results section generally contains a substantial amount of measurements and records in
the form of numerical data. It should be noted that the results section only contains the
results of the experiment, the experimenter does not explain or interpret these results until
the next section is reached.
Discussion
In this section, the data is interpreted and the meaning of the results is explained. This
section concludes whether or not the experiment was successful in testing what it set out
to test, and whether or not the hypothesis was correct or incorrect. It may also discuss how
results correlate with other studies, and discuss how the experiment may be able to be
improved upon in the future.
Literature Cited
Any citations that are used throughout the paper are found in this section. The manner in
which a citation is formated is dependent on the specifications of the university or scholarly
journal in which the report is published. If you are a student writing your own Scientific/Lab
report, you should ask your professor how he or she wants the literature cited entries
formatted.
Tables
This section contains the numerical data that the experimenter gained from the experiment.
These tables may have been referenced and used to explain things in other sections of the
report.
Figures & Graphs
In order to make data more visually appealing, and understanding by the reader, it is
generally formatted into graphs. These should be high quality graphs of data that are
referenced to explain concepts in other portions of the paper.
Review
Biology is the study of life. The different levels at which life can be studied is known as
Biological Order. Every living thing is made up of cells, and cells come from preexisting cells.
There are two types of cells – primitive prokaryotic cells and more complex eukaryotic cells.
There are seven properties that all forms of life share in common. Biology is studied through
the use of the scientific method, and experiments are explained through experimental reports
CHAPTER SUMMARY
Chapter 01: Themes of Biology
Biological Order
Cell Theory & History
General Cell Properties & Types
The Properties of Life
Evolution & Natural Selection
Studying Biology With Experiments
Experimental Ethics & Forbidden Experiments
Scientific Writing
Review
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in scientific journals.