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Test Rapid Brucella Ab Test Kit

A qualitative immunochromatographic assay for the detection of Brucella antibodies in Bovine serum, plasma or whole blood.

20 x Tests/Kit

Rapid Brucella AbTest Kit

Intended use

LilliTest Rapid Brucella Ab Test is a qualitative

immunochromatographic assay for the detection of Brucella

antibodies in Bovine serum, plasma or whole blood. It is only

intended for initial screening and reactive samples should be

confirmed by a supplemental assay such as ELISA.

Principle

LilliTest Rapid Brucella Ab Test works on chromatographic

immunoassay. Basic components of test strip includes: a)

Conjugate pad, which contains Detection Antibody, colloidal gold

conjugated; b) a nitrocellulose membrane strip containing two lines

“T” Brucella abortus LPS and “C” Goat Anti Mouse.

Test sample that is added to the sample well, with adequate amount

of buffer migrates from the sample pad along the conjugate pad

where any IgG present in the sample will bind to the colloidal gold

conjugate. The sample then continues to migrate across the

membrane until it reaches the capture zones where the antibody-

antibody conjugate complex will bind to the immobilized Brucella

abortus LPS antigen (on test line) producing a visible line on the

membrane at “T”. If the respective antibody is not present in the

sample, no reaction occurs in the capture zones and no test line is

formed in the zone corresponding to Brucella LPS antigen. The

sample then migrates further along the strip until it reaches the

control zone, where it produces a second visible line on the

membrane at “C”. This control line indicates that the sample has

migrated across the membrane as intended.

The control line should always appear if the test procedure is

performed properly and the test reagents of control line are

working.

Test kit components

§ 20 aluminum foil pouches each containing one B. abortus Ab

test card and a desiccant.

§ 2 bottles of assay diluent

§ Instruction leaflet

Storage Stability

The kit can be stored at room temperature (2-30 C) or

refrigerated.

The test kit is stable up to the expiration date marked on the

package label.

Do not freeze and do not store the test kit in direct sunlight.

Precautions

§ Treat the specimen as infectious and handle with standard

biosafety measures.

§ Use within 10 minutes after opening pouch.

§ Do not touch result window.

§ Use only the buffer supplied along with the kit.

§ Do not mix components from different kits.

§ Use only for in‐vitro diagnostic purpose.

§ Wear protective gloves while handling specimens. Clean

up spills thoroughly using an appropriate disinfectant.

§ Treat all specimens, used tests and other contaminated

materials as infectious, and dispose accordingly.

§ Do not use with specimen containing precipitates.

Limitation of the test

As with all diagnostic tests the definitive diagnosis should be

based on all data case history, clinical and laboratory findings

etc. being evaluated by the veterinarian.

For more accuracy of immune status, additional follow-up testing

using other laboratory methods is recommended.

°

Specimen Collection & Preparation

§ Blood Specimen: Collect the whole blood using a syringe or

vacutainer into a container containing anticoagulants such as

heparin, EDTA or sodium citrate by venipuncture.

§ Serum: Collect the whole blood using a syringe or vacutainer (NOT

containing anticoagulants such as heparin, EDTA or sodium

citrate) by venipuncture. Leave the syringe or vacutainer,

preferably at an angle, to settle for 30 minutes. Once blood

coagulates, centrifuge the blood to get serum specimen as

supernatant.

§ If the specimen is not used for testing immediately, they should be

refrigerated at 2~8°C.

§ For storage period longer than 5 days, freezing is recommended.

Store at -20°C.

§ The specimen should be brought to room temperature prior to use.

Test Procedure

Allow all kit components and specimen to reach room temperatures

prior to testing.

1.) Take out the test card from the foil pouch and place it on a

horizontal surface.

2.) Add 2 µl of Serum or 5 µl of Whole Blood to the Sample well “S”

3.) When the sample is fully absorbed, add 2 drops of the diluent

provided with the assay to the sample hole.

4.) Wait for 15 minutes and interpret results. The result is considered

invalid after 20 minutes. All results where control band does not

appear are considered invalid.

Interpretation of the results

The presence of two colour bands “T” and “C” within the result window,

no matter which band appears first indicates a positive result.

The presence of only one band at “C” line within the result window

indicates a negative result.

If the control band is not visible within the result window, the result is

considered invalid.