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Mario Guerrero M. Agronomist Engineer, MBA [email protected] Móvil: +56-9-63626913 WWW.NUTRIPROVE.COM

 · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

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Page 1:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

Mario Guerrero M.

Agronomist Engineer, [email protected]

Móvil: +56-9-63626913

WWW.NUTRIPROVE.COM

Page 2:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

Develops and markets solutions for environmental

(abiotic) stresses in all crops.

First products target heat /light /drought stress

management (Suncrops and Oasis), and now cold

stresses (Coldstop).

Focus primarily on high value crops.

Working on both kaolin and non-kaolin solutions.

Operate in a number of countries, like; Mexico, Peru,

Brazil, Chile, Argentina, Central America

Have a Agreement with NUFARM CHILE-AUSTRALIA

(Fresh-Nufresh, Oasis).

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Agriculture for many years lose to much with

invisible abiotic stress.

Buchanan, Gruissem, Jones: Biochemistry and Molecular Biology of Plants; American Society of Plant Physiologists, 2000, FAO

Record Yield(Highest yield reported)

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Abiotic stress

�Heat�Cold�Acidity�Salinity�Radiation

Yie

ldto

n/h

a

Sugarbeet Potatoe Wheat Corn

Abiotic

Biotic

Yield

Crops

Abiotic Stress and Yield

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Biotic V/S Abiotic Stress

Biotic stresses are caused by living organisms:

Insects

Diseases

Weeds

Animals

Abiotic stresses are environmental:

Temperature (heat, cold)

Light (ultraviolet, infra-red , white - low & high intensity)

Water (drought, flood)

Soil conditions (acidity, alkalinity, minerals, toxins etc.)

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The heat, light and water stress

Poor plant establishment

increased re-planting.

prolonged time to bearing in tree fruits.

Lower yield of high quality fruit

flower and fruit set issues.

reduced sizing, despite thinning.

twinning and reduced flower primordia (stone fruit).

Decreased water use efficiency

Sunburn

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Light

H2O

CHO

CHO

CO2

X

X

X

When temperature reaches critical level

photosynthesis slows and eventually stops ¡¡

No Carbohydrate

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Less carbohydrate may produce

Smaller fruit (Pome)

Fewer fruit (increased fruit drop) (Grapevines)

Reduced shoot and root growth

Poor development of reproductive tissues (stone fruits) number

and quality of next season's flowers and periodicity.

Then you have; less carbohydrate and < productivity

And low returns.

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Impact of Heat, Light, Water on crops

All essential for plant growth and crop production

Too much or too little can cause problems

Too little water can cause plants to overheat

Too much light raises temperatures and increases

water use

Too high temperatures impairs photosynthesis and

increases water loss from the crop and soil

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What happens?

Light radiation (UV, visible, IR) is energy.

Excess energy added to plants is converted to heat.

Plants use water in an attempt to keep cool.

At high temperatures, plants begin to malfunction, systems shut down and Shut down systems cause damage to the crop.

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Effects on Photosynthesis. As temperatures increase, then photosynthesis decreases

Photo-inhibition affects electron transport.

Capture of CO2

is decreased.

Chloroplasts continue to absorb light.

Light energy that is not used in photosynthesis is

converted to free radicals (Oっぶ .

Free radicals ふOっぶ damage leaf tissues and others

Plants use stored carbohydrate to repair the damage

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Early stages of development , most important

Many fruit characteristics are fixed during the first 10

weeks after fertilisation, during cell division:

Fruit size and shape

Quality (brix, sugar/acid ratio, juice and oil content, colour, Size)

Critical temperature for photosynthesis in most temperate and tropical fruit crops is around 30C

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Using; New kaolin products (Suncrops) was

sunburn reduction in the last stage of heat

and light stress on crops.

Nutriprove not commercial trials have shown

that losses to �not visible� stress are greater

than to sunburn in many crops..

Nutriprove have new technologies, to control

stress; Oasis and Coldstop-Scaldfree.

We work on the issue:

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Thecnology of Suncrops is different

works in two ways

(1) Stress avoid; kaolin film reflects UV/VIS/IR

radiation, then cooler crops.

(2) Metabolic stress reduction; How? Stimulates and

prepare the plants to stress situations under own

metabolic mechanisms. activate the enzymes that

detoxify ROS, and protect chloroplast membranes.

(ROS-Next Slide).

(3) (2) is a technology in Oasis.

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Reactive oxygen species (ROS) Are chemically reactive molecules containing

oxygen. Examples include oxygen ions and peroxides.

ROS form as a natural byproduct of the normal

metabolism of oxygen and have important roles in

cell signaling and homeostasis.

However, during times of environmental stress

(e.g., UV or heat exposure), ROS levels can

increase.This may result in significant damage to cell

structures. Cumulatively, this is known as oxidative

stress. ROS are also generated by exogenous sources.

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Page 17:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

The evolution of photosynthetic organisms resulted in a

gradual, but massive, increase in the level of atmospheric

molecular oxygen (O2).

All life on earth is based on redox reactions (reduction; the

gain of an electron and oxidation; the loss of an electron,

using reductive processes to store energy.

And oxidative processes to release it. As a result of its

unusual chemistry, it was possible to integrate highly reactive

oxygen in life-giving redox metabolism.

Partial reduction of oxygen produces REACTIVE OXYGEN

SPECIES (ROS) including superoxide anion (O2 -), hydrogen

peroxide (H2O2) and the hydroxyl radical (OH·).

Page 18:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

The plant antioxidative system is continuously processing ROS, by acting

as electron donors the antioxidants are themselves oxidised in the process

of neutralising the ROS.

This regulates the accumulation of ROS by determining their lifespan

and spatial distribution and by controlling signal specificity. Just as a vast

number of mechanisms for the generation of ROS exist, as do antioxidative

compounds contributors to the plant antioxidative system include

superoxide dismutase (catalyses the generation of oxygen and H2O2 from

superoxide), catalase (decomposition of H2O2 to water and oxygen),

ascorbate (vitamin C, water soluble providing protection in the cytosol and

appoplast), tocopherols vitamin E, (lipid soluble antioxidants that provide

protection to lipid membranes), thioredoxin (promotes active metabolism

and detoxifies ROS by reducing disulphide bonds), and glutathione (GSH,

maintains ascorbate and thiol/disulphide balance).

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Redox homeostasis, is maintained in plants by pools of antioxidants,

such as ascorbate and glutathione and anothers (Oasis).

The antioxidants are generally maintained in their 'active' reduced

state by specific enzymes, like glutathione reductase.

The balance of reduced and oxidised antioxidants can be utilised by

plants to detect changes in the environment via changes in ROS

concentrations.

For example, ROS generation by the chloroplast, the major source of

ROS, can be increased by excess light, drought, salt stress and in some

cases conditions of limiting carbon dioxide by activation of the

photorespiratory pathway resulting in H2O2 production in the

peroxisomes. The ROS will oxidise the antioxidants and the redox balance

will be disrupted.

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Using Suncrops in extreme conditions

January - Temp 33 Celcius, under shade.

Control Treated

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ADVANTAGES OF USING SUNCROPS

� Early & season-long applications, for thereduction of heat, light and water stress anddamage in to a range of crops.

� Label rates of Suncrops are ½ and lowest ifcompare with traditional kaolins.

� Trials was conducted by universities, in many countries (see on web www.nutriprove.com)

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Slight damage

Moderate damage

Severe damage

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LEVELS OF DAMAGE

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Reduces Sunburn

Pink Lady San Fernando Chile

0

2

4

6

8

10

12

14

16

UNTRATED CONTROLSURROUND SUNCROPS SCREEN DUO PURSHADE

Pe

rce

nt su

nb

urn

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POTENTIAL YIELD

SUNCROPS USE EFFECT IN ENHANCING PERFORMANCE,

MITIGATING PHYSIOLOGICAL STRESS AND SUN DAMAGE IN

APPLE CV. PINK LADY, SN FDO. CHILE 2010

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BENEFICIAL USE OF SUNCROPS

Improvements in the efficiency of domestic water use of the plant.

Floral abortion reduction and improves fruit Induction.

In cherry, cherries reduces the incidence of twins.

Reduced water stress in plants cherry postharvest.

Fruits of better quality and reduced fruit drop. Alternating reduced production.

Increased yield and quality associated with an improvement in crop plant

health.

No fruit stain; formulation not clog nozzles fine, nice suspension.

Low dust granulometry.

Page 28:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

TRADITIONAL KAOLIN V/S

SUNCROPS

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Kaolin v/s Calcium carbonate, source ?

Sunblock range UV Calcium Carbonate 5-20 %

Sunblock range UV caolín 32-60 %

Kaolin; Surround

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Apples

6 applications

every treatments

(Rate)

fruit (mm)

Diameter

% Fruit with no

sunburn

exportable

Quality

fruit weight

gr/apple

Yield

kg/Tree

Exportable

fruits

Harvest

Kg/Tree

control 0 36,90 76,80a 134,0 37a 28,4a

Suncrops 1.25 % 39,81 89,80b 152,0 45b 40,8b

Screen duo 2.5% 39,16 88,50b 151,0 43b 38,8b

SUNCROPS V/S COMPETITORS

Fuente; Fruits Chilean Company, Private .

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Suncrops effect acting on apples

San Fernando 2010, Pink Lady

8 8 applicationsapplications / ha/ ha

Total Total RateRate 200 200 kg/hakg/ha

Page 32:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

Cherry-TreeImproved floral induction.

Reducing double fruits.

Post harvest with high temperatures (see next picture)

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EFFECT OF USING SUNCROPS IN EARLY POSTHARVEST IN

CHERRY-TREE POSTHARVEST 1.25% FIRST APPLICATION, 2011.

Leaves without abiotic

stress

Leaves with stress

SUNCROPS

CONTROL

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2ND APPLICATION, 25 DAYS, SUNCROPS RATE 1,25%

SUNCROPS

CONTROL

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SUNCROPS TREATMENT MONITORING, APRIL

SUNCROPS

CONTROL

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ANGOL, APPLES VAR. PINK LADY,

SUNCROPS

CONTROL

SUNCROPS

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PINK LADY, APPLICATION SUNCROPS, 2010-11

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SAN FERNANDO, PINK LADY, GRANNY

Damage on

control

TREATED CONTROL

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SUNCROPS SURROUND

DIFFERENCE BETWEEN TRADITIONAL

KAOLIN AND SUNCROPS

Page 40:  · temperate and tropical fruit crops is around 30C Using; New kaolin products (Suncrops) was ... , hydrogen peroxide (H2O2) and the hydroxyl radical (OH·). The plant antioxidative

HAZELNUT; SUNCROPS; BLANCA MESSINA

ADVISOR AND FARMER PROVIDER FERRERO-ROCHER

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SUNCROPS; BLUEBERRIES, ALTO PELARCO, CHILE

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SUNCROPS EN WALNUT, ALTO PELARCO, TALCA, CHILE

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CONTROL DE STRESS HIDRICO

DEFICIT DE AGUA DE RIEGO, OLIVES

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EFFECT ON EXPORT QUALITY

OF APPLES

Place: San Fernando; 2011

Pink Lady

a

b

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GRAPEVINES-VINEYARD ANS WINERY

SUNCROPS-FRESH CONTROL

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Wine Grape Heat/Sunburn Stress Rating

System

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Heat Stress Damage on leaves

San Pedro Vineyard & Winery. Trial Talca University 2011

CONTROL SUNCROPS-FRESH

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TEMPERATURES ON THE SEASON

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SHADE

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APPLICATIONS

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Calendar application and Nufresh dose during test

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CONTROL V/S NUFRESH

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SUNBURN PERCENTAGE

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LEVELS OF DAMAGE

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TEMPERATURES ON THE SEASON

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Number of

application

1° 2° 3° 4° 5° 6°

Date 05/12/12 21/12/12 04/01/13 24/01/13 14/02/13 05/03/13

APPLICATION DATES

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TREATMENT SUNBURN LEVELS (%)

Healthy Slight damage Serious injury

Control 31,07 a 44,29 a 24,64 a

Sprayer 38,87 ab 39,55 a 21,58 ab

Bar or Tower 43,46 b 39,83 a 16,71 b

Incidence of sunburn in Granny Smith apple fruits treated with respect to the

control SUNCROPS Different letters in the column indicate significant

differences (P <.05).

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LEVELS OF DAMAGE

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Percentage of fruits damaged by sunburn, Control and treatments

with applied SUNCROPS. With spray compare to a bar. Different

letters on the bars of the graph indicate significant difference (P <0.05)

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Treatment Weight

(g)

Diameter

(mm)

Flesh

firmness

(Lbs.)

Soluble

solids

(ºBrix)

Index

starch

(%)

Control 203,6 a 77,8 a 18,1 a 11,8 a 20 a

Sprayer 212,7 a 79,1 a 17,4 a 11,7 a 27 a

Bar or

Tower

205,1 a 78,1 a 19,2 b 12,4 a 25 a

Morphological and quality index of maturity in Granny Smith apple

fruit. SUNCROPS treated compare to control.

Different letters in the column indicate significant differences (P <.05).

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Treatment Chlorophyll

(SPAD)

Dry matter

(mg/cm2)

Foliar area

(cm2)

Control 43.75 a 11.37 a 19.12 a

Sprayer 42.87 a 10.89 a 18.85 a

Bar or Tower 42.92 a 11.04 a 19.52 a

Vegetative variables in Granny Smith apple trees, treated compare with the

control SUNCROPS.

Different letters in the column indicate significant differences (P <.05).

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SUNCROPS SUNSCREEN, RESULTS IN

REDUCTION OF SUNBURN IN APPLE

cv. GRANNY SMITH.

2012 CURICO, CHILE

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TEST PROTOCOL

Objective: To evaluate effectiveness of Suncrops on sunburn in apples

� Variety: Granny Smith� Area: 0.25 ha� Treatments: Control = T0; Suncrops = T1� Dosage: 1 2.5% and 1.25% implementation in 2nd, 3rd and 4th application.� Volume of water used: 500 liters / 0.25 ha� Application Equipment: Nebulizer� Application Start Date: 12/12/2011� Application End Date: 14/02/2012� Parameters to Measure:

a)Percentage of fruit with sun damageb)level sunburn in orchard and packing process.

c)Caliber curve.

� Evaluation Date: March (field) - July (packing).

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SUNCROPS

NUMBER AND DATE OF APPLICATION.

Cost Suncrops: $US 5,5/kg.

Cost/ há, for this treatment : US$ 687,4

(Old trees, Very high volume of water 2000 L/Ha)

Total : 31,25 kg 171,86 U$

(0,25 há) (0,25 há)

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Total harvest in kg and total kg with sunburn damage

(Sunburn) in orchard of apples cv. Granny Smith.

Field San Ramon, SOLFRUT, Curico, Chile

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Percentage of sun damage in to orchard

Percentage of sunburn in apples cv. Granny Smith

in quality control Orchard San Ramon - Solfrut.

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Packing, percentage of sunburn

Percentage of sunburn in apple cv. Granny Smith, in process

packing at Copefrut S.A. Orchard San Ramon-Solfrut

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Percentage of sunburn (orchard +

packing)

Percentage of total sunburn (orchard + packing) in apple cv. Granny Smith

Orchard San Ramon, Solfrut, Curico, Chile

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Fruit caliber curve in apple fruit cv. Granny

Smith, at san Ramon orchard-Solfrut at packing

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Results, Analisys

With Suncrops treatment (T1) reach 4.3% of more fruits from orchardinto packing process, for fewer sunburn.

With T1 would be a 2.7% more fruit packed at packing, for less amountof fruit-sunburn at commercial fruit.

With T1 there altogether 7% less of sunburn (orchard + packing) tocompare with T0. which reach a total sunburn of 13.6%.

Caliber curves show that, the curve of T1 is more shifted to largercalibers. In this case, the caliber peak is 80 on contrast with T0 , thatreach the peak at caliber 100.

In the caliber T1 weigh average reach 91 (198 g/fruit) and at T0,caliber weighted average are 94 (192 g / fruit).

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Calculations of kg won, by less damage of

sunburn with apply of Suncrops

The Kg of fruits produced, from orchard to packing. With Suncrops

treatment (T1) was 13,904 kg (0.25 ha) taking it and carry on to production

per hectare, would be are 55,616 kg x 39.29% DN. (DN =% packaging

within norm) = 21,851 kg / ha.

the case of T0 would be 53,224 kg / ha from orchard to process (4.3% +

sunburn) and multiplying by a standard percentage in 36.59% (was

subtracted 2.7% of more sunburn), gives a value of 19,474 kg / ha.

The difference between 21,851 (DN T1) and 19,474 (DN T0) = 2,377 kg /

ha.

There are a 2,377 kg / ha over than T1, for less surburn.

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Appearance of fruits on to trees, treated with Suncrops

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Conclusions

There are a 7% less sunburn in T1, if compare with T0.

It would be have at pack on packing, over 3% more kg of

commercial fruits. Because have a less impact of sunburn.

The curve of size distribution is better for T1, where the larger

sizes are observed with an 91 caliber average and weight

average are near to 198 gr.

The initial date to improve the treatments, was too late

(December). Is very important began these, early on November

and must be respect the dose of 75 kg/Ha, which are a perfect

dose, to reduce costs to farmers.

In addition, are recommended to good apply, use a bar or

tower to increase efficiency; and also reach a high coverage on

fruits.

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Different technology to traditional physical barriers, kaolins,

carbonates.

OASIS individual effectiveness in preventing heat damage

and water stress in all crops.

Synergistic action together with SUNCROPS FRESH or, in

reducing sunburn.

Product powder dosage and application is easy.

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Treatments Product Dose (L/ha)

T0

T1

Water

OASIS

0

20

lt/aplication

TREATMENT IN TABLEGRAPES

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Variable Control OASIS Control OASIS Control OASIS Control OASIS

22/11 07/12 21/12 04/01

Temperature(°C)

Morning

Mid-day

Evening

14,5

37,6

29,5

13,9

36,9

28,0

12,8

33,2

37,1

12,4

31,8

36,6

13,7

32,4

35,6

12,9

31,1

33,4

13,5

28,7

29,8

12,8

27,6

29,0

HydricPotential MPa

Morning

Mid-day

Evening

-0,32

-0,61

-0,50

-0,25

-0,55

-0,45

-0,18

-0,50

-0,60

-0,15

-0,40

-0,50

-0,22

-0,58

-0,75

-0,18

-0,51

-0,67

-0,21

-0,61

-0,82

-0,17

-0,52

-0,75

Temperature and water potential in leaves of

variety Sultanina (Thompson seedless)

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Treatment Yield

(kg/plant)

Weight

bounch

(g)

Weight

berry

(g)

Ecuatorial caliber

(mm)

Control

OASIS

21,4

22,7

794,1

841,5

5,0

5,4

18,8

19,2

Yield and fruit quality in variety Sultanina

(Thompson seedless)

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Treatment Soluble solids

(°Brix)

Acidity

(%)

Ph

Control

OASIS

18,0 a

18,7 a

0,92 a

0,80 b

3,18 a

3,00 b

Features grape juice in variety Sultanina

(Thompson seedless)

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Treatment Bronzed Bounches (%)

Control

OASIS

87

25

Incidence of clusters tanned in variety Sultanina

(Thompson seedless)

The information indicates that the OASIS product had a positive effect on the color

of the berries.

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BENEFITS IN ALL CROPS

Approach allows the potential crop

Improving crop performance in severe environmental stress

Improved quality and yield of fruits

Technology excellent cost / benefit

Usable in all crops

Suitable for all types of weather

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CENTER-AMERICA

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DIFFERENT CROPS

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ARTIFICIAL COVERING

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PINEAPPLES CONTROL

SLIGHT SEVERE

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MOST SUSCEPTIBLE STAGE

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Status of Suncrops TRIALSFinca: Caño Sucio. Mr Miguel Alfaro

Fruits /treatment: 50 fruits

Fruit Evaluated: 20 fruits

Applicación to: 60 days before forza (ddf) y 90 days before de forza (ddf)

Rate: 50kg/ha , 60 ddf y 37,5 kg/ha , 90 ddf (Suncrops 1/2dose)

Treatment % Sunburn Status % Fruit without damage

Surround WP 0% Sana 100%

Suncrops (1/2

dose) 0% Sana 100%

Control 10% Leve 90%

Another trial: Week 24, 2013

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84%

88%

92%

96%

100%

Sana Sana Leve

0% 0% 10%

Surround WP Suncrops Testigo

Po

rce

nta

je d

e f

ruta

sin

da

ño

de

qu

em

a d

e

so

l

Tratamientos

Surround WP 0% Sana Suncrops 0% Sana Testigo 10% Leve

RESULTS IN PINEAPPLES

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FRUITS WITH SUNCROPS APPLICACTION

THAN MORE 20 MM RAIN

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MANGO-BANANA: Apply 1.25 kg/100 liters of water, every 10-14 days after

petal fall. If it Hight rains or high temperatures, re-apply. Apply up to 15 days

prior to harvest. Last applying OASIS to 250 g / ha.

a) MANGO

1.-uniformity of shape and size, skin color (depending on cultivar) and

flesh firmness.

2.-Presence of sunburn (incidence)

3.-Conversion of starch to sugar (increased tenderness)

4.-decreased acidity and increased carotenoids and aromatics.

5.-texture (fiber).

b) BANANA

1.-Maturity (the more mature the better quality when ripe)

Two. - Finger length (depending on its intended use and the demand for

various sizes.

3.-Conversion of starch into sugars (sweetness).

4.-Incidence sunburn.

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PINEAPPLE:.

Apply 25 kg / ha, just start developing fruit. Reapply at intervals of

14 days up to 40 days before harvesting. After (last applicaction)

applied every 15 days at doses of 250 OASIS gr / ha

MEASURE:

1.-Uniformity of size and shape, firmness.

2.-Absence of sunburn. (Incidence)

3.-crown Leaves: green, medium length and straight.

4.-soluble solids range, 11-18%.

5.-titratable acidity (as citric acid) 0.5-1.6%.

June. - Ascorbic acid (vitamin C), 20-65 mg/100 g fresh weight.

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TOMATO and CAPSICUM: 25kg/ha application initial post 12.5kg/ha applications,

from fruit ripening, up to 15 days before harvesting. Last applying OASIS to 250 g /

ha

a) TOMATO

1.-The size is not a factor that defines the degree of quality, but can influence so

important in commercial quality expectations.

2.-uniform color (orange-red to deep red, light yellow). No green shoulders.

3.-Sunburn (Incidence)

4.-Firmness-Firm to the touch. Not soft nor easily deformed due to over mature.

5.-Soluble Solids and Acidity

b) CAPSICUM

1.-Shape, size and uniform color typical of the cultivar

2.-Firmness

3.-Sunburn (Incidence)

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CITRUS: applying 12.5 kg / ha initial subsequent applications to 0.625 kg / ha,

from petal fall and fruit ripening, up to 20 days before harvesting.

MEASURE:

1.-Quality Sensory juice content, size, texture, color, maturity index, aroma etc.

2.-Nutritional quality is the amount of vitamin C, amount of protein, sugar-

acid balance etc.

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Tomato for industry

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Sunburn Tomato

CONTROL Surround Suncrops

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THANKS A LOT¡¡