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Effects of Alcohol on a 5 Day Old Chick Embryo’s Heart Rate
Tami ChristTalia Katz
Biology 240WPenn State Lehigh Valley
Background InformationAlcohol can have a good effect on the
heart.Moderate consumption can help protect
against heart disease.It can help raise HDL (good cholesterol)
which helps to prevent plaque in arteries.
Background InformationAlcohol can have a
negative effect on the heart.
Heavy consumption can lead to multiple diseases and heart complications.
Some disease and complications include: hypertension, alcoholic cardiomyopathy, congestive heart failure, and stroke.
Null HypothesisThe null hypothesis of our experiment was
that the alcohol would not have an effect on the heart.
Choosing the ConcentrationsWe chose the alcohol concentrations
based on the amount of alcohol in everyday items.
30%, which is the amount in mouthwash.
10%, which is the amount in Nyquil.5%, which is the average amount in
beer.
Making the ConcentrationsIn order to make the concentrations, started off
with 80 proof alcohol (40% alcohol).Then we used the 40% alcohol and gradually
decreased the concentration using chick saline.30% = 3 parts alcohol and 1 part chick saline.10% = 2 parts 30% alcohol and 1 part chick
saline.5% = 1 part 10% alcohol and 1 part chick saline.
ProcedureFirst, place tape on
egg.Then puncture a
hole in egg with a scissors and withdraw some of the fluid from egg with syringe.
Cut out the window to reveal the embryo.
ProcedureLeave chick embryo
in egg.While in the egg, be
sure to count the heart rate in vivo (bpm) with a stop watch.
Then explant the embryo from the egg with a tiny spoon and place in a dish filled with chick saline.
ProcedureThen move the
embryo to a dish filled with CMRL.
Now count the heart rate in vitro (bpm).
Once the in vitro heart rate is taken, then explant the heart from the embryo’s body.
ProcedureOnce the heart is
explanted and the in vitro heart rate is counted, submerge the embryo’s heart in one of alcohol solutions.
Count the heart rate once placed into the dish of the alcohol solution.
Data TableHeart Rate –
In vivoHeart Rate –
In vitro (explanted)
Heart Rate – After
Exposure
Embryo 1 (30%)
152 24 Stopped (0)
Embryo 2 116 Stopped Stopped (0)
Embryo 3 140 Stopped Stopped (0)
Embryo 4 (10%)
124 92 Stopped almost
instantly (0)
Embryo 5 120 68 Stopped before exposed (0)
Embryo 6 (0.05%)
120 52 16Table 1
Heart Rate vs. State of Heart
In V
ivo
In V
itro
(exp
lant
ed)
After e
xpos
ure
0
20
40
60
80
100
120
140
160
Embryo 1 (30%)Embryo 2 Embryo 3 Embryo 4 (10%)Embryo 5 Embryo 6 (0.05%)
State of the Heart
Heart
Rate
(B
eats
per
Min
ute
)
Future Experiments & RevisionsIn future experiments, 1 change should be
made.The 3 concentrations of alcohol would
need to be change.The concentrations were way too high and
stopped the heart almost immediately.Changing the concentrations to ideal blood
alcohol levels ( 0.08% < x < 0.08%).
SummaryWe really did not get a clear answer to our
hypotheses.I think with a future experiments and the
revisions made we would have a clearer answer to how alcohol and its concentration affect the heart.
With the information gained from the experiment, we have a better idea for future experiments.
References“Alcohol and Heart Disease.” Women’s Heart Foundation.
2007. <http://www.womensheart.org/content/HeartDisease/alcohol_and_heart_disease.asp>.
McLaughlin, Jacqueline, McCain, Elizabeth. “Student Outline.” 1999. <http://www2.lv.psu.edu/jxm57/chicklab/outline.html>.
Norr, Serena. “How Does Alcohol Affect the Heart?” 2009. <http://i.ehow.com/images/a04/m8/58/alcohol-affect-heart-800X800.jpg>.