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China • France • Germany • India • Singapore • Switzerland • United Kingdom • United States www.biotek.com/take3
BioTek Instruments, Inc.Tel: 802-655-4040 • Toll-Free: 888-451-5171 • Outside the USA: 802-655-4740
www.biotek.com
The Only Micro-Volume
Multi-Sample
Multi-Mode
Multi-Volume
Multi-Tasking Plate Available Today
Take3™
measuringmicro-volumesamplesjustgotfasterandeasier.
Pre-programmed nucleic acid and protein
protocols within the custom Gen5™ Take3
software interface make quantification fast
and easy with results displayed automatically.
Take3 can also be used for a variety of
assays that might be run in a 96- or 384-
well microplate, saving precious samples
with low volume capability. The BioCell
and cuvette measurement locations allow
rapid 1 cm direct measurements of nucleic
acids, proteins or nearly any analyte typically
measured in a traditional spectrophotometer.
Researchers today look for ways to maximize productivity and minimize costs wherever possible. Minor bottlenecks
in the workflow can cause major delays in downstream processes. One of the most effective means of keeping the
workflow moving and keeping costs down is to use instrumentation that can serve multiple users and applications
and minimize the volume of reagents and samples required. Microplates, primarily 96- and 384-well, have aided assay
miniaturization and increased throughput, and are the standard for a vast number of biomolecular applications.
Newer technologies are available that allow even smaller, micro-volume samples to be measured in key areas like
nucleic acid and protein quantification.
www.biotek.com/take3
Features• Sixteen 2 μL sample microspots on a custom designed and printed fused silica surface
• Absorbance and fluorescence measurement capability
• Simply pipette and read
• 1 standard cuvette and 2 BioCell measurement locations
• Pre-programmed applications in the Gen5™ Take3 software module
• Sample surfaces are easy to clean, and are easily replaceable
Gen5 Take3 Interface
Sample Spectrum
Concise Results Display
Applications• Micro-volume nucleic acid quantification • Micro-volume protein quantification • A280 • In-situ fluorometric protein quantification • 260/280, 260/230 protein purity measurements
• Very low volume In-situ BCA assay • Spectral scanning of single or multiple low volume samples• Fluorescent dye quantification • Gene expression assays• Cell culture measurements• Endpoint and kinetic assays
SpecificationsSamplingLocations
Microspot:Up to sixteen 2 μL samples can be run at one time for direct nucleic acid and protein quantification
BioCell: BioCell locations available for quick 1 cm pathlength measurements
Cuvette:A standard stoppered cuvette can be measured
Note: BioCell and cuvette are not included with Take3
DetectionModesTake3 can be used for absorbance and fluorescence measurements including wavelength scanning
CompatibleReadersEpoch™, PowerWave™ XS2 and all Synergy™ Hybrid and Multi-Mode readers equipped with absorbance and/or fluorescence detection
SampleVolumeMinimum 2 μL in microspot locations
OpticalPathlength0.5 mm nominal
DetectionLimit2 ng/μL (dsDNA) typical (spectrophotometric)
Gen5SoftwareInterfaceComes with Take3 module, compatible with Gen5 and Gen5 Secure. Includes predefined applications: dsDNA, ssDNA, RNA & protein quantification
BioTek’sTake3™Multi-Volume,Multi-SamplePlateis designed to increase
the functionality of your BioTek microplate spectrophotometer, multi-mode or Hybrid Reader by decreasing
the sample size required to a mere 2 µL! This very small volume allows for quick quantification of
nucleic acid and protein samples without dilution…saving time and valuable samples. For added
utility, standard cuvettes and the patented 1 cm BioCell™ can also be used for quick
measurements. For the ultimate versatility, both absorbance and fluorescence can be
measured with Take3 on a Synergy™ Hybrid or multi-mode reader.