Table S1. Primers for quantitative RT-PCR

β-actin Sense 5'-ATCTGGCACCACACCTTC-3'

Antisense 5'-AGCCAGGTCCAGACGCA-3'

CD36 Sense 5'-TGGTCAAGCCAGCTAGAAA-3'

Antisense 5'-CCCAGTCTCATTTAGCCAC-3'

TTP Sense 5'-CGGAGGACTTTGGAACAT-3'

Antisense 5'-GCGAAGTAGGTGAGGGTG-3'

a b

Fig. S1

1 2 3 4 5 60

2

4

6VehicleIMD

(Weeks)

Fo

od

inta

ke (

g/2

4h)

0 1 2 3 4 5 60

10

20

30

40VehicleIMD

(Weeks)

Bo

dy

wei

gh

t (g

)

a

(400×)

Control IMD HFS HFS+IMD

bControl IMD HFS HFS+IMD

Filipin staining

Fig. S2

Oil red O staining

Control IMD HFS HFS+IMD0.0

0.5

1.0

1.5

*

Rel

ativ

e fl

uo

resc

ence

IMD - - + + - - + +

PPAR

-actin

Cytoplasm Nuclear

a

b

0 5 10 20 50 (nM)

IMD (24h)

p-PPAR

PPAR

0 4 8 12 24 (h)

IMD (20nM)

p-PPAR

PPAR

Fig. S3

0 4 8 12 240.0

0.5

1.0

1.5

IMD (20 nM)

(h)

p-P

PA

R

pro

tein

leve

l

0 5 10 20 500.0

0.5

1.0

1.5

IMD (24h)

(nM)

p-P

PA

R

pro

tein

leve

l

- + - +0.0

0.5

1.0

1.5

Cytoplasm Nuclear

IMD (20nM)

PP

AR

pro

tein

leve

l

c d

a b

Fig. S4

0 5 10 20 500.0

0.5

1.0

1.5

IMD (8 h)

(nM)

TT

P m

RN

A le

vel

0 1 2 4 80.0

0.5

1.0

1.5

IMD (20 nM)

(h)

TT

P m

RN

A le

vel

-actin

0 1 2 4 8 (h)

IMD (20 nM)

TTP

0 1 2 4 80.0

0.5

1.0

1.5

IMD (20 nM)

(h)

TT

P p

rote

in le

vel

-actin

0 5 10 20 50 (nM)

IMD (8 h)

TTP

0 5 10 20 500.0

0.5

1.0

1.5

IMD (8 h)

(nM)

TT

P p

rote

in le

vel