T. Trimpe 2005 http://sciencespot.net/

Base

-Single piece of metal-Support here when moving

Color RED

Arm

-Hold here when moving

Color Dark GREEN

Stage

-Supports Specimen-Hole allows light to pass through

Color BLUE

Diaphragm

-controls amount of light-ranges 1-5

Color LIGHT GREEN

Stage Clips

-secures specimen/slide

Color GREY

Bulb/Mirror

-light source

-NEVER use the sunlight it will hurt your eyes!!

Color YELLOW

Scanning Objective Lens

-shortest lens-4x magnification

Color BLACK

Low Power Objective Lens

-10x magnification

Color PURPLE

High Power Objective Lens

-40x magnification

Color ORANGE STRIPES

Body Tube

-allows light from objective to pass upward

Color BROWN

Eye Piece/Ocular lens

-Site of observation-10x magnification

Color ORANGE

Nose Piece

-Allows you to switch objective lenses

Color BLUE and GREEN stripes

Coarse Adjustment Knob

-used to focus specimen-DO NOT use on high power objective lens

Color PINK

Fine Adjustment Knob

-Used for minute focusing -Used after coarse adjustment knob

Color Tan

• Total magnification = (ocular lens power) x (objective lens power)

• Example: (10x)oculear lens x (40x)high power lens = 400x total magnification

• Magnification: The ability to increase the size of an image

Ocular Lens Objective Lens

= 400x total magnification

X

Light Pathway: Light Source→Specimen→Objective Lens→Body Tube→Eye Piece

Resolution

• making the image clear

• ability to determine between two objects or points

• can be adjusted with the fine/course knob or the diaphragm (to allow more light in)

Comparing Powers of Magnification

We can see better details with higher the powers of magnification, but we cannot see as much of the image.

Which of these images would be viewed at a

higher power of magnification?

Use of a Scope Carry the microscope with 2 hands (arm & base)

Use closest electrical outlet without going through a walkway

Looking at a slide:

Use ONLY ONE slide at a time ALWAYS start at LOW power first(10x) ALWAYS start with the stage lowered completely Make sure the objective clicks into position The course focus knob is ONLY used on the lowest

power lens The fine focus knob is used on all lenses to bring the

image into focus

Putting the Microscope Away

Lower the stage completely and click the lowest objective into place

Return slide to tray Wind up the scope power cord

Agenda

1. Finish Microscope Handout

• Glue in notebook when finished

• Due tomorrow

2. Study Guide

• Answer on separate piece of paper

• Due Friday

3. Extra Credit Flashcards

• Make 20

• Due Friday

Types of Microscopes

1. Compound Light Microscope: Shines light through a specimen and uses 2 lenses for magnification

Magnification – increase in an objects apparent size

Resolution – how clear the image appears once magnified

Max clear magnification ~2000x

2. Electron microscope: Uses electrons instead of light to visualize the specimen, electrons bounce off or pass through and a computer interprets a picture

Always black and white, specimen must be dead to examine

Scanning EM – magnifies up to 100,000x

Transmission EM – magnifies up to 200,000x

Microscope Images

Compound Light Microscope - Protozoa

Transmission Electron Microscope - Bacteria

Scanning Electron Microscope (SEM)Butterfly Tongue