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Suveshnee Munien [email protected]

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Page 1: Suveshnee Munien munien@ukzn.ac - MILE

Suveshnee Munien

[email protected]

Page 2: Suveshnee Munien munien@ukzn.ac - MILE

Based on random sampling and structured data collection. ◦ that fit diverse experiences into predetermined response

categories. ◦ produce results that are easy to summarize, compare,

and generalize.

Testing hypotheses derived from theory and/or being able to estimate the size of a phenomenon of interest.

Typical strategies : ◦ Experiments/clinical trials ◦ Observing and recording ◦ Obtaining relevant data from information systems ◦ Administering surveys with closed-ended questions

(Interviews & questionnaires)

Page 3: Suveshnee Munien munien@ukzn.ac - MILE

Validity: the degree to which a study accurately reflects or assesses the specific concept that the researcher is attempting to measure. ◦ The study’s success at measuring what the researcher

set out to measure.

◦ Internal (the research design) and external (generalisation and transferability).

Reliability: the accuracy of the actual measuring instrument or procedure. ◦ Reliability: the extent to which an experiment, test, or

procedure yields the same result on replication.

Page 4: Suveshnee Munien munien@ukzn.ac - MILE

‘Assumption’ based on what you expect your research to refute or accept.

Null (Ho) and alternate (Ha).

Causality ◦ ‘smoking causes cancer’

Test for relationship ◦ ‘long term smoking increases the risk of cancer’

Not all research needs an hypothesis but should have questions you wish to address

Page 5: Suveshnee Munien munien@ukzn.ac - MILE

Research phenomena under investigation

Research questions

Study aims and objectives

Research design and methodology ◦ Experimental

◦ Field survey and observation

Sampling framework ◦ Probability sampling

Margin of error

Page 6: Suveshnee Munien munien@ukzn.ac - MILE

Experimental/ clinical trials ◦ Rigour and accuracy ◦ Replication and method is crucial ◦ Researcher has the ability to influence and control

variables

Field survey and observation ◦ Limited control of variables by researcher ◦ Similar to case study- using a reality to understand how

different aspects are linked and/ or impact each other

Page 7: Suveshnee Munien munien@ukzn.ac - MILE

Cross-sectional studies: understanding variables/ phenomena within a specified reality. ◦ Not time dependent

Longitudinal studies: examine the phenomena over a certain time period ◦ Time-series analyses

◦ Seasonality studies

Page 8: Suveshnee Munien munien@ukzn.ac - MILE

Researcher has minimal influence on the selection of individual units- Objectivity

Probability - All units in that population has an equal opportunity of being selected. ◦ Random selection of units

Non-probability- selection criterion that defines how units are chosen within a population. ◦ Units are systematically or purposively selected

Dependent on your research aim/ intention

Page 9: Suveshnee Munien munien@ukzn.ac - MILE
Page 10: Suveshnee Munien munien@ukzn.ac - MILE

Quantitative assessments Qualitative assessments

Measurable ◦ Turbidity

◦ pH

◦ E.coli levels

Observation ◦ Colour

◦ Odour

◦ Surrounding environments

Cross-sectional and longitudinal

Page 11: Suveshnee Munien munien@ukzn.ac - MILE

Longitudinal studies

Page 12: Suveshnee Munien munien@ukzn.ac - MILE
Page 13: Suveshnee Munien munien@ukzn.ac - MILE

Measure varaibles Interpretation

Page 14: Suveshnee Munien munien@ukzn.ac - MILE

Measurable • Root & shoot length • Height • Leaf chemistry

Observation • Colour • Occurrence of

disease • Shape of leaves

Page 15: Suveshnee Munien munien@ukzn.ac - MILE

Measureable ◦ Income

◦ Energy expense

◦ Access

Observation ◦ Cooking practices

◦ Ventilation during cooking

◦ Collection of energy sources

Table 5.1 Household income (in %)

Categories Inanda (n=400) Bergville

(n=400)

None 2.8 4

< 1000 12.7 24.7

1001-3000 48.1 62.2

3001-5000 21 8.7

5001-7000 9.1 1.8

7001-9000 3 0.8

> 9001-15000 3.4 -

Average household

income R3109.25 R1841.38

Area Inanda

(n=400)

Bergville

(n=400)

Indoors 98.5 98.3

Outdoors 1.5 1.3

Table 5.2: Main cooking area (in %)

Cooking method Inanda

(n=400)

Bergville

(n=400)

Stove top 98.3 81.5

Fire top 1.8 42.3

Table 5.3: Main cooking method (in %)

Page 16: Suveshnee Munien munien@ukzn.ac - MILE

Figure 5. Energy status of Bergville communities

Page 17: Suveshnee Munien munien@ukzn.ac - MILE
Page 18: Suveshnee Munien munien@ukzn.ac - MILE

Should be clear and detailed – not précised as in journal article

Must provide details of replication, type of data collected and explain how data was processed (where necessary)

Must indicate after whom the method was adapted from (according to/ after)

Be sure to link to experimental design (e.g. on what day of the experiment the measurements were taken)

Flow diagram or design diagrams may be useful in some cases

* See examples

Page 19: Suveshnee Munien munien@ukzn.ac - MILE

(1) Results or Results and (2) Discussion

In (1) results are reported and interpreted - not explained, nor linked to broader literature

In (2) results are reported, interpreted, explained and linked to broader literature - usually in that order

Text associated with a figure or table should always precede it

Avoid including results of statistics in text (refer to Fig. or Table)

Avoid reporting marginal differences rather focus on significant and/or broad trends

When saying higher/lower/worse/better – always remember to relate it to something (e.g. the control)

Page 20: Suveshnee Munien munien@ukzn.ac - MILE

Should incorporate replicate numbers

Title should describe data shown

Legend/Caption should indicate what the values represent, statistical tests (+p values) and replication

Treatment abbreviations should be expanded

Page 21: Suveshnee Munien munien@ukzn.ac - MILE

Figure 7.8 Instantaneous leaf-based CO2-assimilation rates (at Ca: 400 µmol. mol-1) of seedlings recovered from fresh (F), partially dried (D) and cryopreserved (C) embryos, subjected to watering (W) or water deficit (S). Columns labelled with different letters are significantly different when compared within experimental days (p < 0.01 for Embryo on days 0 and 12 and = 0.01 on day 8; p = 0.04 for Stress and = 0.01 for EmbryoStress on day 8, ANOVA, n = 7). Bars represent ±SD.

0

2

4

6

8

10

12

0 8 12Time (days)

CO

2 a

ssim

ilatio

n (

µm

ol m

-2 s

-1)

FW DW CW FS DS CS

a

bb

a

abc

bc

bc

ab

c

cdc

c

b

a

cdd

Page 22: Suveshnee Munien munien@ukzn.ac - MILE

48.8

14.6

29.3

48.8

17.1

65.2

43.5

13

34.8 34.8

0

10

20

30

40

50

60

70

Cheaper Safe to use No negative healthimpacts

Environmentallyfriendly

Reliable

%

Inanda (n=99) Bergville (n=98)

Figure 5.15: Perceived benefits of renewable energy

Page 23: Suveshnee Munien munien@ukzn.ac - MILE

Ratio was calculated for seedlings recovered from fresh (F), partially dried (D) and cryopreserved (C) embryos, subjected to watering (W) or water deficit (S). Values followed by different letters are significantly different when compared within a measurement day (p < 0.01 for Embryo on days 8 and 12 and for Stress and EmbryoStress on day 12; p < 0.05 for Stress and EmbryoStress on day 8, ANOVA). Values represent mean±SD (n = 7). NA = not applicable.

Table 7.3 Ratio of CO2-assimilation rate at Ca: 600 µmol. mol-1 : CO2-assimilation rate at Ca: 400 µmol. mol-1.

Day FW DW CW FS DS CS

0 2.29±0.60a 2.11±0.55

a 1.85±0.35

a NA NA NA

8 1.68±0.44b 1.66±0.70

b 1.81±0.35

b 2.48±0.23

a 1.57±0.15

b 1.76±0.47

b

12 1.68±0.10b 1.61±0.17

b 1.51±0.15

b 2.36±0.53

a 1.66±0.45

b 1.43±0.20

b

Page 24: Suveshnee Munien munien@ukzn.ac - MILE

Statements Inanda (n=400)

Strongly

disagree

Disagree Neutral Agree Strongly

agree

Mean

Is expensive 3 9.3 4 30 53.8 4.39

Is bad for

health 5 6.3 25.5 25.3 38 4.06

Is unreliable 14 15.3 36.6 18.3 16 3.17

Causes

environmental

pollution

12 15.3 12.3 27 33.8 3.62

Inefficient 29 35.8 16 10 9.3 1.83

Is inaccessible 13 11.3 22.3 27 26.8 3.56

Page 25: Suveshnee Munien munien@ukzn.ac - MILE

20

22

24

26

28

30

32

34

36

38

0 7 14 21 28 35 42 49

Mid

da

y a

ir t

emp

era

ture

(oC

)

Days After Initiation

Control OTC

Page 26: Suveshnee Munien munien@ukzn.ac - MILE

0

2

4

6

8

10

Control pH 4.5 pH 3.0

Chlo

rophyll

conte

nt cm

-2

Treatments

Chl a Chl b Total chlorophyll

a

a

ab

a

b

a

aab

b

Page 27: Suveshnee Munien munien@ukzn.ac - MILE

Working with raw data and means

Statistics always on raw data

Pool data from different experiments except where it is done over different seasons or populations

Export into SPSS by copying and pasting from excel file

Page 28: Suveshnee Munien munien@ukzn.ac - MILE

Parametric versus non-parametric (KS-test; Shapiro Wilks)

Transforming data (log, square root transform) – only for analysis; not for figs and tables

Percentiles need to be arcsin transformed Parametric = ANOVA, T-test Non-parametric = Mann Whitney-U Correlation/Regression to test for

relationships between parameters - p-value tells you when relationship is significant; R2 describes strength of relationship

Page 29: Suveshnee Munien munien@ukzn.ac - MILE