Upload
others
View
1
Download
0
Embed Size (px)
Citation preview
Table 1: Details of individuals used in present study and their respective location.
Sample name Voucher
Number
Locality GenBank Accession numbers
cyt b 12S RAG-1 PDC
Cyrtodactylus
ayeyawardyensis
CAS
216446
vic. Kanthaya Beach, Rakhine State,
Myanmar
- - EU268287 EU268317
Cyrtodactylus consobrinus LLG 4062 Niah Cave, Sarawak, Malaysia - - EU268288 EU268318
Cyrtodactylus loriae FK 7709 N slope of Mt. Simpson, Bunisi,
Milne Bay Province, Papua New
Guinea
- - EU268289 EU268319
Hemidactylus agrius E1708.2 Dom Expedito Lopes, Piaui, Brazil DQ120261 DQ120432 - -
Hemidactylus angulatus 1 E1708.15 Kajiado District, Rift valley, Kenya DQ120241 DQ120412 - -
Hemidactylus angulatus 2 20HbrSt Santiago, Cape Verde islands, Cape
Verde
DQ120230 DQ120401 - -
Hemidactylus angulatus 3 E1708.12 Malabo, Bioko island, Equatorial
Guinea
DQ120218 DQ120389 - -
Hemidactylus bouvieri 1 2HbouBo Boavista, Cape Verde islands, Cape
Verde
DQ120251 DQ120422 - -
Hemidactylus bouvieri 2 1HbouS Sal, Cape Verde islands, Cape Verde DQ120247 DQ120418 - -
Hemidactylus bowringii 1 E1109.6 Shan State, Myanmar DQ120285 DQ120456 - -
Hemidactylus bowringii 2 E170810 Nujiang Prefacture, Yunnan, China DQ120286 DQ120457 - -
Hemidactylus bowringii 3 E1708.8 Mingalardon Township, Yangon
Division, Myanmar
DQ120287 DQ120458 - -
Hemidactylus bowringii 5 CAS
228109
Liuku, Nujang District, Yunnan
Province, China
- - EU268313 EU268343
Hemidactylus bowringii 6 CAS
206649
Alaungdau Kathapa Natl. Park,
Sagaing Division, Myanmar
- - EU268312 EU268342
Hemidactylus brasiliana E1209.4 Dom Expedito Lopes, Piaui, Brazil DQ120257 DQ120428 - -
Hemidactylus brasilianus MZUSP
92493
Parque Nacional Serra das
Confusões, Piauí, Brazil
- - EU268290 EU268320
Hemidactylus brookii 1 Hemb22b Mauritius DQ120272 DQ120443 - -
Hemidactylus brookii 10 LLG 6755 Empan-gon Air Hitam, Pulau
Pinang, Malaysia
- - EU268305 EU268335
Hemidactylus brookii 11 LLG 6754 Empan-gon Air Hitam, Pulau
Pinang, Malaysia
- - EU268304 EU268334
Hemidactylus brookii 12 CAS
206638
Mandalay Division, Myanmar - - EU268314 EU268344
Hemidactylus brookii 2 E1109.11 Kollam, Kerala, India DQ120273 DQ120444 - -
Hemidactylus brookii 3 E1708.17 Mingalardan, Yangon Division,
Myanmar
DQ120274 DQ120445 - -
Hemidactylus brookii 4 E1708.18 Kyauk Pan Tawn, Mandalay DQ120275 DQ120446 - -
Division, Myanmar
Hemidactylus cf. angulatus 4 MVZ
245438
Nkwanta, Togo Hills, Nigeria - - EU268306 EU268336
Hemidactylus fasciatus 1 E1708.21 Luba, Equatorial Guinea DQ120265 DQ120436 - -
Hemidactylus fasciatus 2 WRB no
number
Rabi, Gabon - - EU268309 EU268339
Hemidactylus fasciatus 3 CAS
207777
3.6 km N of Luba, Bioko Island,
Equatorial Guinea
- - EU268310 EU268340
Hemidactylus flaviviridis 1 E1708.22 Fujairah, UAE DQ120283 DQ120454 - -
Hemidactylus flaviviridis 2 E912.2 Hadibu, Socotra island, Yemen DQ120284 DQ120455 - -
Hemidactylus flaviviridis 8 FMNH
245515
Punjab Province, Pakistan - - EU268294 EU268324
Hemidactylus flaviviridis 9 ID 7626 Kuldhara, Rajasthan, India - - EU268295 EU268325
Hemidactylus frenatus 1 E509.6 Kauai, Hawaii islands, USA DQ120277 DQ120448 - -
Hemidactylus frenatus 10 AMB 7420 Rathegala, Sri Lanka - - EU268298 EU268328
Hemidactylus frenatus 2 E509.7 Kauai, Hawaii islands, USA DQ120278 DQ120449 - -
Hemidactylus frenatus 3 E509.3 Puerto Carreno, Colombia DQ120279 DQ120450 - -
Hemidactylus frenatus 4 E509.1 Mwe Hauk, Ayeyarwade Division, DQ120280 DQ120451 - -
Myanmar
Hemidactylus frenatus 5 E509.2 Mandalay Division, Myanmar DQ120281 DQ120452 - -
Hemidactylus frenatus 6 E509.5 Thiruvananthapuram, India DQ120282 DQ120453 - -
Hemidactylus frenatus 8 LLG 6745 Empan-gon Air Hitam, Pulau
Pinang, Malaysia
- - EU268297 EU268327
Hemidactylus frenatus 9 AMB 7411 Pidipitiya, Sri Lanka - - EU268296 EU268326
Hemidactylus garnotii 1 E509.20 Alaungdaw Kathapa, Sagaing
Province, Myanmar
DQ120288 DQ120459 - -
Hemidactylus garnotii 2 E509.10 Dade Co., Florida, USA DQ120290 DQ120461 - -
Hemidactylus garnotii 3 E509.8 Yangon Division, Myanmar DQ120292 DQ120463 - -
Hemidactylus garnotii 4 CAS
223286
Ma Ei Ywa Ma Village, Taung Gok
Township, Rakhine State, Myanmar
- - EU268302 EU268332
Hemidactylus garnotii 5 CAS
222276
Kyait Hti Yo Wildlife Sactuary,
Mon State, Kyaihto Township,
Myanmar
- - EU268303 EU268333
Hemidactylus greeffii 2 CAS
219044
Praia da Mutamba, São Tome
Island, São Tome and Principe
- - EU268308 EU268338
Hemidactylus greefii 1 E7014.4 Principe, Sao Tome and Principe DQ120243 DQ120414 - -
Hemidactylus haitianus 1 HhaitiS Matanzas, Matanzas province, Cuba DQ120217 DQ120388 - -
Hemidactylus haitianus 2 CAS
198442
near Santo Domingo, Nacional Dist.,
Dominican Republic
- - EU268311 EU268341
Hemidactylus imbricatus 1a JS 11 Pakistan (captive specimen) EU268385 EU268292 EU268322
Hemidactylus imbricatus 1 a A. M.
Bauer29(A)
captive animal DQ852724
Hemidactylus imbricatus 2 a JFBM 2 Pakistan (captive specimen) - - EU268293 EU268323
Hemidactylus karenorum 1 E509.19 Mandalay Division, Myanmar DQ120293 DQ120464 - -
Hemidactylus karenorum 2 CAS
210670
Popa Mt. Park, Kyaukpadaung
Township, Mandalay Division,
Myanmar
- - EU268301 EU268331
Hemidactylus longicephalus E7014.5 Sao Tome´, Sao Tome and Principe DQ120245 DQ120416 - -
Hemidactylus mabouia 1 E609.9 Praia da Pipa, Rio Grande do Norte,
Brazil
DQ120204 DQ120375 - -
Hemidactylus mabouia 2 32HbouSV Sao Vicente, Cape Verde islands,
Cape Verde
DQ120186 DQ120357 - -
Hemidactylus mabouia 3 MCZ R-
184446
Limpopo Province, South Africa - - EU268300 EU268330
Hemidactylus modestus E1109.7 Kijiado District, Rift Valley
Province, Kenya
DQ120215 DQ120386 - -
Hemidactylus palaichthus LSUMZ H- Roraima State, Brazil - - EU268307 EU268337
12421
Hemidactylus persicus 1 E7014.12 4.5 km N. of Tanuf, Wilayat Nizwa,
Oman
DQ120166 DQ120337 - -
Hemidactylus persicus 3 CAS
227612
Wadi Tanuf, 4.5 km N. of Tanuf,
Wilayat Nazwa,Oman
- - EU268316 EU268346
Hemidactylus platycephalus1 E609.16 Kijiado District, Rift Valley, Kenya DQ120270 DQ120441 - -
Hemidactylus platycephalus2 E609.15 Kajiado District, Rift Valley, Kenya DQ120269 DQ120440 - -
Hemidactylus platyurus 1 E29104.2 Gon Nyin, Sagaing Division,
Myanmar
DQ120294 DQ120465 - -
Hemidactylus platyurus 2 E29104.5 Alaungdaw Kathapa NP, Sagaing
Division, Myanmar
DQ120295 DQ120466 - -
Hemidactylus platyurus 3 E29104.4 Khim Aye Village, Sagaing
Division, Myanmar
DQ120296 DQ120467 - -
Hemidactylus platyurus 4 KU 304111 Lubang, Philippines - - EU268291 EU268321
Hemidactylus robustus MVZ
248437
40 km South of Mipur Sakro, Thatta
District, Pakistan
- - EU268315 EU268345
Hemidactylus turcicus 1 E28104.10 Dair al Khaf, Jordan (vouhcer in
National Museum, Prague)
DQ120165 DQ120336 - -
Hemidactylus turcicus 2 LSUMZ H-
1981
Baton Rouge, Louisiana, USA - - EU268299 EU268329
Hemidactylus yerburyii E1109.12 Najran, Saudi Arabia DQ120207 DQ120378 - -
Pachydactylus turneri Pcla Richtersveldt, NW Cape Province,
South Africa
AF184990 AF186118 - -
Tarentola americana Tamer Guanta´namo, Cuba AF184991 AF186119 - -
Tarentola boettgeri 1 CA11GC Arinaga, Gran Canaria, Canary
islands, Spain
AF184997 AF186125 - -
Tarentola boettgeri 2 CA17H Los Llanillos, El Hierro, Canary
islands, Spain
AF184999 AF186127 - -
‘a’ indicates the Hemidactylus species restricted to Indian subcontinent.
Table 2: List of Primers used:
Primer Sequence Length
Cytochrome b (cyt b)
CB1L-14817 5’-CCA TCC AAC ATC TCA GCA TGA TGA
AA-3’
303 bp
CB2H-15175 5’-CCC TCA GAA TGA TAT TTG TCC TCA-3’
12S ribosomal RNA (12S)
12S L1201 5'- AAA CTG GGA TTA GAT ACC CCA CTA
T- 3'
350 bp
12S H1486 5'-GAG GGT GAC GGG CGG TGT GT-3'
Recombination activating gene-1 (RAG-1)
RAG1 F700 5’-GGAGACATGGACACAATCCATCCTAC-3’ 650 bp
R18 5’-GATGCTGCCTCGGTCGGCCACCTTT-3’
R13 5’- TCTGAATGGAAATTCAAGCTGTT-3’ 650 bp
RAG1 R700 5’-TTTGTACTGAGATGGATCTTTTTGCA-3’
Phosducin (PDC)
PHOF2 5’-AGATGAGCATGCAGGAGTATGA-3’ 450 bp
PHOR1 5’-TCCACATCCACAGCAAAAAACTCCT-3’
Table 3: Thermo cycler profile used for amplification of genes.
PCR Product Length PCR Profile
Mitochondrial Genes
cyt b 303 bp 94°C: 5 minutes, (94°C: 30 seconds, 48°C: 45 seconds, 72°C: 1 minute)
x 35 cyles, 72°C: 5 minutes, Hold at 4°C
12S 399 bp 94°C: 5 minutes, (94°C: 30 seconds, 48°C: 45 seconds, 72°C: 1 minute)
x 35 cyles, 72°C: 5 minutes, Hold at 4°C
Nuclear Genes
RAG-1 650 bp (94°C: 3 minutes, 48°C or 55°C: 45 seconds, 72°C: 1 minute),(94°C:
30 seconds, 48°C or 55°C: 45 seconds, 72°C: 1 minute) x 35 cyles,
72°C: 6 minutes, Hold at 4°C.
PDC 450 bp 95°C: 12 minutes, (94°C: 1 minute, 56°C: 1 minute, 72°C: 1 minute) x
35 cyles, 72°C: 5 minutes, Hold at 4°C.