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Supplementary File 1
Supplementary Figure S1 ………………………………………………………………… Page 2
Supplementary Figure S2 ………………………………………………………………… Page 3
Supplementary Figure S3 ………………………………………………………………… Page 4
Supplementary Figure S4 ………………………………………………………………… Page 5
Supplementary Figure S5 ………………………………………………………………… Page 6
Supplementary Figure S6 ………………………………………………………………… Page 7
Supplementary Figure S7 ………………………………………………………………… Page 8
a) b)
2
Supplementary Figure S1. Formica fusca larva eating an egg. a) before eating the egg b) after eating the egg.
a) b)
3
Supplementary Figure S 2. Size of Formica fusca larvae used in this study. a) eggs in the top row, pupae in the bottom row, and larvae of increasing size in between b) magnified view of the upper four rows, the bottom two rows representing the larvae used in this study.
Number of GO-terms for Sequences with Length(x) [fusca]
0 2,000 4,000 6,000 8,000 10,000 12,000 14,000 16,000 18,000 20,000Length
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
#GO
Length
Num
ber G
Ote
rms
4Supplementary Figure S3. Relationship between the length of the unigenes and the number of GOterms associated.
GO-Level Distr ibution [fusca]
P F C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
GO Level (Total Annotations = 34402, Mean Level = 6.36, Std. Deviation = 2.528)
0
250
500
750
1,000
1,250
1,500
1,750
2,000
2,250
2,500
2,750
3,000
3,250
3,500
3,750
#Ann
otat
ions
GO-Level Distr ibution [fusca]
P F C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
GO Level (Total Annotations = 34402, Mean Level = 6.36, Std. Deviation = 2.528)
0
250
500
750
1,000
1,250
1,500
1,750
2,000
2,250
2,500
2,750
3,000
3,250
3,500
3,750
#Ann
otat
ions
GO-Level Distr ibution [fusca]
P F C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
GO Level (Total Annotations = 34402, Mean Level = 6.36, Std. Deviation = 2.528)
0
250
500
750
1,000
1,250
1,500
1,750
2,000
2,250
2,500
2,750
3,000
3,250
3,500
3,750
#Ann
otat
ions
GO-Level Distr ibution [fusca]
P F C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
GO Level (Total Annotations = 34402, Mean Level = 6.36, Std. Deviation = 2.528)
0
250
500
750
1,000
1,250
1,500
1,750
2,000
2,250
2,500
2,750
3,000
3,250
3,500
3,750
#Ann
otat
ions
Biological processMolecular functionCellular components
GO Level
Num
ber o
f ann
otat
ions
5
Supplementary Figure S4. Distribution of hierarchical level of GO annotations. For each annotation level, the number of sequences mapping to a term is shown for biological process, molecular function and cellular components. Total annotation = 34402, Mean level of GOterm = 6.36, Deviation = 2.53.
Direct GO Count (BP) [fusca]
0 500 1,000 1,500 2,000 2,500 3,000 3,500 4,000 4,500 5,000#Seqs
oxidation-reduction processproteolysis
regulation of transcription, DNA-templatedtransmembrane transport
signal transductionDNA integration
protein phosphorylationphosphorylation
nucleic acid phosphodiester bond hydrolysismetabolic process
protein ubiquitinationG-protein coupled receptor signaling pathway
translationintracellular signal transductioncarbohydrate metabolic process
ubiquitin-dependent protein catabolic processdetection of chemical stimulus involved ...
microtubule-based movementmethylation
peptidyl-tyrosine phosphorylationbiosynthetic process
DNA repairintracellular protein transport
DNA replicationRNA-dependent DNA biosynthetic process
lipid metabolic processpositive regulation of GTPase activity
translational initiationcell redox homeostasis
multicellular organism developmentchitin metabolic process
DNA recombinationnitrogen compound metabolic process
primary metabolic processRNA processing
transposition, DNA-mediatedDNA metabolic process
regulation of Rho protein signal transductionion transmembrane transport
vesicle-mediated transportmacromolecule metabolic process
protein foldingprotein transport
response to stimuluspotassium ion transmembrane transport
regulation of transcription by RNA polymera...peptidyl-tyrosine dephosphorylation
transcription, DNA-templatedDNA biosynthetic process
others
GOG
o te
rms (
Biol
ogic
al p
roce
sses
)
Number of sequences
6Supplementary Figure S5. GO annotation classification of the top 50 biological processes.
Direct GO Count (MF) [fusca]
0 500 1,000 1,500 2,000 2,500 3,000 3,500 4,000 4,500 5,000 5,500#Seqs
ATP bindingnucleic acid binding
metal ion bindingzinc ion binding
DNA bindingRNA binding
calcium ion bindingtransferase activity
oxidoreductase activitykinase activityheme binding
sequence-specific DNA bindingprotein kinase activity
DNA-binding transcription factor activityiron ion binding
protein dimerization activityGTP binding
hydrolase activityligase activity
monooxygenase activitystructural constituent of ribosome
oxidoreductase activity, acting on pair...serine-type endopeptidase activity
bindingcatalytic activity
microtubule motor activityodorant binding
endonuclease activityolfactory receptor activity
transmembrane transporter activityATPase activity
nucleotide bindingGTPase activity
methyltransferase activityhelicase activity
protein serine/threonine kinase activityG-protein coupled receptor activity
actin bindingflavin adenine dinucleotide binding
microtubule bindingtranslation initiation factor activity
hydrolase activity, acting on ester bondsRNA-directed DNA polymerase activity
ubiquitin-protein transferase activityGTPase activator activity
aspartic-type endopeptidase activitypyridoxal phosphate bindingmetalloendopeptidase activity
chitin bindingothers
GO
Number of sequences
Go
term
s (M
olec
ular
func
tion)
7Supplementary Figure S6. GO annotation classification of the top 50 molecular functions.
Direct GO Count (CC) [fusca]
0 500 1,000 1,500 2,000 2,500 3,000 3,500#Seqs
integral component of membranenucleus
membranecytoplasm
intracellularplasma membrane
extracellular regionribosome
mitochondrionubiquitin ligase complex
cellendoplasmic reticulum membrane
dynein complexcytoskeleton
Golgi membranecytosol
microtubulemitochondrial inner membrane
nucleolusmyosin complex
endoplasmic reticulumextracellular space
motile ciliummediator complex
chromosomeperoxisome
integral component of plasma membranehistone acetyltransferase complex
Golgi apparatusproteasome complex
transcription factor complexchloroplast
collagen trimermicrotubule organizing center
spliceosomal complexpostsynapse
COPII vesicle coatmitochondrial matrix
mitochondrial outer membraneproton-transporting V-type ATPase, V1 domain
nuclear poreeukaryotic 43S preinitiation complex
cytosolic large ribosomal subuniteukaryotic 48S preinitiation complex
large ribosomal subunitmeiotic nuclear membrane microtubu...
transcription factor TFIID complexvoltage-gated potassium channel complex
centrosomeothers
GO
Number of sequences
Go
term
s (Ce
llula
r com
pone
nt)
8Supplementary Figure S7. GO annotation classification of the top 50 cellular components.