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Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences (5’→3’) Size（bp） Rabbit GAPDH GTCATCATCTCAGCCCCCTC 99 GGATGCGTTGCTGACAATCT COL1A1 GCGGTGGTTACGACTTTGGTT 139 AGTGAGGAGGGTCTCAATCTG COL2A1 CAGGCAGAGGCAGGAAACTAAC 132 CAGAGGTGTTTGACACGGAGTAG COL10 ATCAGCCACTGGGAAGCC 72 TTCGGTCCACTTGGTCCTC ACAN ATGGCTTCCACCAGTGCG 127 CGGATGCCGTAGGTTCTCA SOX9 GTACCCGCACCTGCACAAC 100 TCCGCCTCCTCCACGAAG CCNA2 TGACTTAGCTGCACCAACGG 195 CCCGTGACTGTGTAGAGTGC MCM6 CAAGACAGGGACATCGCACT 178 GAGGTCCAGGCATGGTAAGG RAD21 GACTCACCCAGTTCACCCAG 128 CACAAACTGGGTTTCGGCAG STAG1 TTGCGAACATCGGGACATCT 246 GCTGAATTGTTGGGCCACAT EXO1 CAGCTTCTTCGTGAGGGGAA 111 TCCCTGAGACCTAGCAGCTT human GAPDH CTATAAATTGAGCCCGCAGC 143 GACCAAATCCGTTGACTCCG COL1A1 GCTTCACCTACAGCGTCACT 154 AAGCCGAATTCCTGGTCTGG COL2A1 TGAGCCATGATTCGCCTCG 110 CCCTTTGGTCCTGGTTGCC COL10 CGCTGAACGATACCAAATGCC 258 TTCCCTACAGCTGATGGTCC ACAN CTACACGCTACACCCTCGAC 216 ACGTCCTCACACCAGGAAAC SOX9 AAGCTCTGGAGACTTCTGAACG 133 CGTTCTTCACCGACTTCCTCC CCNA2 CTGGTGGTCTGTGTTCTGTGA 137 TGCCAGTCTTACTCATAGCTGA MCM6 CCAGTGGTAAAGCGTCCAGT 268 CCACTGATTGGGTTTGCTGC RAD21 GCTGCCGCAAAGTTCTACAG 101 GGTCCAGGTGTTGCGATGAT STAG1 ACAGATGCTGTACACCGTTCA 183 GGCAGGGCATGAAATAAGTGG

Supplemental Table 1. Sequences of primers used in real-time … · 2017-06-01 · Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences

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Page 1: Supplemental Table 1. Sequences of primers used in real-time … · 2017-06-01 · Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences

Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences (5’→3’) Size（bp）

Rabbit

GAPDH GTCATCATCTCAGCCCCCTC 99 GGATGCGTTGCTGACAATCT

COL1A1 GCGGTGGTTACGACTTTGGTT 139 AGTGAGGAGGGTCTCAATCTG

COL2A1 CAGGCAGAGGCAGGAAACTAAC 132 CAGAGGTGTTTGACACGGAGTAG

COL10 ATCAGCCACTGGGAAGCC 72 TTCGGTCCACTTGGTCCTC

ACAN ATGGCTTCCACCAGTGCG 127 CGGATGCCGTAGGTTCTCA

SOX9 GTACCCGCACCTGCACAAC 100 TCCGCCTCCTCCACGAAG

CCNA2 TGACTTAGCTGCACCAACGG 195 CCCGTGACTGTGTAGAGTGC

MCM6 CAAGACAGGGACATCGCACT 178 GAGGTCCAGGCATGGTAAGG

RAD21 GACTCACCCAGTTCACCCAG 128 CACAAACTGGGTTTCGGCAG

STAG1 TTGCGAACATCGGGACATCT 246 GCTGAATTGTTGGGCCACAT

EXO1 CAGCTTCTTCGTGAGGGGAA 111 TCCCTGAGACCTAGCAGCTT

human

GAPDH CTATAAATTGAGCCCGCAGC

143 GACCAAATCCGTTGACTCCG

COL1A1 GCTTCACCTACAGCGTCACT

154 AAGCCGAATTCCTGGTCTGG

COL2A1 TGAGCCATGATTCGCCTCG

110 CCCTTTGGTCCTGGTTGCC

COL10 CGCTGAACGATACCAAATGCC

258 TTCCCTACAGCTGATGGTCC

ACAN CTACACGCTACACCCTCGAC

216 ACGTCCTCACACCAGGAAAC

SOX9 AAGCTCTGGAGACTTCTGAACG

133 CGTTCTTCACCGACTTCCTCC

CCNA2 CTGGTGGTCTGTGTTCTGTGA

137 TGCCAGTCTTACTCATAGCTGA

MCM6 CCAGTGGTAAAGCGTCCAGT

268 CCACTGATTGGGTTTGCTGC

RAD21 GCTGCCGCAAAGTTCTACAG

101 GGTCCAGGTGTTGCGATGAT

STAG1 ACAGATGCTGTACACCGTTCA

183 GGCAGGGCATGAAATAAGTGG

Page 2: Supplemental Table 1. Sequences of primers used in real-time … · 2017-06-01 · Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences

EXO1 AAACCTGAATGTGGCCGTGT

113 CCTCATTCCCAAACAGGGACT

Page 3: Supplemental Table 1. Sequences of primers used in real-time … · 2017-06-01 · Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences

Supplementary figures

Figure. S1 HE staining of repaired cartilage in vivo at different

post-transplantation time, respectively. Scale bar: 400μm.

Page 4: Supplemental Table 1. Sequences of primers used in real-time … · 2017-06-01 · Supplemental Table 1. Sequences of primers used in real-time PCR Species Genes Primer sequences

Figure. S2 Safranin-O staining of repaired cartilage in vivo at different

post-transplantation time, respectively. Scale bar: 400μm.

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