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Supplemental Material, Quan et al. contains:
Supplemental Figures S1 to S7
Supplemental Document 1 and 2
Supplemental Tables IX, X, XI, XV, XVI
Supplemental Figure S1
Supplemental Figure S1. ICL activity of Arabidopsis
seedlings after growing 3 to 6 days. Three technical repeats
were used. Error bars are standard deviations.
0
5
10
15
20
25
30
35
40
3d 4d 5d 6d
ICL a
ctivity (
U/m
g p
rote
in)
Supplemental Figure S2
200 kDa
100
75
50
37
25
20
150
Supplemental Figure S2.
Silver-stained gel for the
immunoblot shown in Figure
1D. CE, crude extract; P1,
pellet fraction after the first
gradient centrifugation: PE,
highly enriched peroxisomes
after the second gradient
centrifugation. After Bradford
measurement of protein levels,
15 µg of proteins were loaded
in each lane.
CE P1 PE
Supplemental Figure S3
Supplemental Figure S3. Subcellular distribution of proteins identified from
total peroxisomes (T1 and T2) in this study based on protein number.
T2 (n=135)
Cytosol: 7.4%
Nucleus: 3.7%
Mitochondria: 5.2%
Plastid: 40%
Secretory system: 11.9%
Plasma membrane: 0.7%
Peroxisome: 31.1%
T1(n=147)
Cytosol: 11.6%
Nucleus: 2%
Mitochondria: 4.8%
Plastid: 27.2%
Secretory system: 19.7%
Plasma membrane: 5.4%
Peroxisome: 29.3%
Supplemental
Figure S4
YFP-UP3 (SSL>) YFP-UP2 (SLN>) YFP-BADH (SKL>)
YFP-AAE5 (SRM>)
Overla
y
YF
P f
usio
n
CF
P-P
TS
1
YFP-SDRd (SSL>)
YFP-ATMS1 (SAK>)
A B C
YFP-GOX3 (AKL>)
Supplemental Figure S2. Verification of peroxisomal localization for six proteins identified from this study and our previous leaf
peroxisomal proteomics but had not been confirmed before.
Confocal microscopic images were taken from leaf epidermal cells of 4-week-old Nicotiana tabacum plants co-expressing YFP
fusions of the candidate proteins and the peroxisomal marker protein CFP-PTS1. Scale bars = 10 µm.
Overla
y
YF
P fusio
n
CF
P-P
TS
1
YFP-GOX3 (AKL>) E F G
8
9 20 16
59
10
Green leaf (Reumann et al., 2009): 105 Cell culture (Eubel et al., 2008): 84
Etiolated seedlings (This study): 77
Supplemental Figure S5. Comparison of the number of
peroxisomal proteins identified from different tissues from
different studies.
Supplemental Figure S5
Supplemental Figure S6
PXN
APX3
NDPK1
ASP3
PEX11e
HIT1
PEX11a
ACX2
GOX1/GOX2
PEX11d
OPR3
MDH2
RDL1
ATMS1
SCPL20
LACS6
ICL
ICDH
ECHIA
IBR10
PMDH1
MLS
CAT2
KAT2
4CL1
IBR1
CSD3
SO
SCP2
SDR
UP9
CHY1
BADH
BZO1
MFP2
LACS7
KAT1
AtDCI
CSY2
ACX4
ACX3
NQR
NS
GAPC2
st1
SDRc
UP3
6PGDH
GGT1
CuAO
HPR1
GOX3
PEX14
SDRb
Uri
ECH2
CSY3
PXA1/CTS
ATF2
ACH2
GSTT1
CAT3
st3
ACX1
ZnDH
AAE5
CAT1
HBCDH
AIM1
IBR3
ACAT1.3
LON2
UP6
AtHsp15.7
GGT2
1 2 3 4 5 6 7
-5 5 0
Supplemental Figure S6. Heatmaps showing relative expression levels of genes encoding peroxisomal
proteins identified from etiolated seedlings in this study. (A) Log2 normalized expression at various
developmental stages. 1, seedling cotyledons; 2, vegetative rosette; 3, flowering rosette; 4, flower stage 12;
5, senescing leaf; 6, seed6-silique; 7, seed10-silique; 8, embryo heart, cotyledon; 9, embryo torpedo,
cotyledon; 10, dry seeds; 11, 24h imbibed seed. (B) Log2 normalized expression during seed germination as
compared with dry seed. 1, fresh seeds; 2, 12h stratified; 3, 48h stratified; 4, 6h light grown; 5, 12h light
grown; 6, 24h light grown; 7, 48h light grown.
ICL MLS UP6 BZO1 LACS7 ECHIA CSY3 GOX3 AtHsp15.7 CAT1 GGT2 MDH1 PXA1 CSD3 UP9 IBR10 ACAT1.3 PEX11a KAT2 GSTT1 ACX1 AAE5 ACX3 ASP3 SCP2 LACS6 MFP2 ACX2 IBR3 CSY2 LON2 ZnDH RDL1 SDRb ATF2 SO HIT1 6PGDH NDPK1 sT1 Scpl20 GAPC2 NS ECH2 PEX11e ACX4 IBR1 PXN NQR ICDH PEX11d APX3 BADH ACH2 sT3 KAT1 UP3 SDRc SDRd CAT3 CAT2 HBCDH AIM1 PEX14 AtDCI 4CLP1 CHY1 Uri CuAO ATMS1 OPR3 GOX1/GOX2 MDH2 GGT1 HPR1
1 2 3 4 5 6 7 8 9 10 11
5 -5 10 0 -10
A B
Supplemental Figure S7
Supplemental Figure S7. Phenotypic analysis of the mutants of the newly validated peroxisomal genes. (A) Seed
germination in response to ABA. Percentage of radicle emergence from seeds 6 days after growing on ½ LS
supplemented with the indicated concentrations of ABA was quantified. Each data point represents the mean (±SE)
of three independent experiments (n=100 per experiment). Mutants in this graph do not show statistically significant
difference from the wild type plant. (B) Sugar dependence assays. Hypocotyl lengths of 7d dark-grown seedlings on
1/2 LS supplemented with or without 1% sucrose are shown. Except for the pex14 control, no statistically significant
differences were found between the mutants and the wild type. (C) IBA response assays. Relative root length (on
IBA vs. w/o IBA) of 7d seedlings on 1/2 LS supplemented with 5 µM, 10 µM, or 15 µM of IBA are shown. Data
represent the mean (±SE) of three independent experiments. For each experiment, n=50. Asterisks indicate
measurements significantly different from the wild type (Student’s t test: one asterisk, p<0.05; two asterisks,
p<0.005). All experiments on gapc2-2 mutant were performed separately.
B
0.0
0.5
1.0
1.5
2.0
2.5
Col-0 Col-3 pex14 bzo1-4 scpl20-1 scpl20-2 gox3-1 rdl1-1 rdl1-2
+ sucrose
- sucrose
Hypo
co
tyl le
ng
th (
cm
)
**
0.0
0.5
1.0
1.5
2.0
2.5
Col-0 gapc2 pex14 Col-0 gapc2-2 pex14
**
gox3-1
0
20
40
60
80
100
120
Col-0 Col-3 pex14 bzo1-4 scpl20-1 scpl20-2 gox3-1
IBA 5µM IBA 10µM IBA 15µM C
Rela
tive
ro
ot g
row
th (
%)
(vs. n
o I
BA
)
** **
**
gox3-1
0
20
40
60
80
100
120
0 0.6 2 5 10
Rad
icle
em
erg
en
ce
(%
)
ABA concentration (µM)
Col-0
Col-3
bzo1-4
scpl20-1
scpl20-2
gox3-1
A
0
20
40
60
80
100
120
0 0.6 2 5 10
gapc2-2
Col-0
Rad
icle
em
erg
en
ce
(%
)
ABA concentration (μM)
gox3-1
0
20
40
60
80
100
120
Col-0 gapc2-2 pex14
** **
Supplemental Document 1. MS/MS spectra for the five known peroxisomal proteins with single peptide identification (all from Z4).
AT
3G
01980
A
T1G
77540
A
T1G
23310
A
T4G
16566
AT
1G
04710
Supplemental document 2. Amino-acid sequences for homologs of RDL1, Scpl20, UP9 and GAPC2 proteins
used in creating alignments shown in Figure 4.
>AtRDL1(At4g36880)
MAPSTKVLSLLLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIIND
QDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAKNVNQKYS
AAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELISLSEQELVDCDKSYN
QGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRVVSIDGYEDVPTKDETALKKAIS
YQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRM
ERNLAASKSGKCGIAVEASYPVKYSPNPVRGNTISSV
>BrRDL1(Bra010592)
MASSTKLLSSLLLFVVVSLASGDEFTTINNHLNLPSDGSWRTDEEVRSIYLQWCAEHGKT
SNNNGIINQQDERFNIFKDNLRFIDLHNENNKNATYKLGLTIFADLTNDEYRRLYLGART
ESVRRITKAKNVNIKYSAAVNDVEVPETVDWRQKGAVNAIKNQGTCGSCWAFSTAAAVEG
INKIVTGELISLSEQELVDCDRSYNQGCNGGLMDYAFQFIMKNGGLNTEQDYPYRGSNGK
CNSLLKNSRVVTIDGYEDVPTEDETALKRAVSYQPVSVAIEAGGRVFQHYQSGIFTGKCG
TNLDHAVVAVGYGSENGIDYWIVRNSWGTRWGEDGYIRMERNLARSKSGKCGIAVEASYP
VKYSPNPVRGTSSV
>RcRDL1(29970.m000973)
MALPISLSTLLFLFFTLSSAWDMSILSHNHGHHHQSSWRSDNEVISMYNWWLAKHSKTYN
KLGEREKRFEIFKNNLRFIDEHNNSKNRTYKVGLTRFADLTNEEYRAKFLGTKSDPKRRL
MKSKNPSQRYAFKAGDVLPESIDWRQSGAVSAIKDQGSCGSCWAFSTIAAVEGVNKIVTG
ELISLSEQELVDCDRSYNAGCNGGLMDNAFQFIINNGGIDTDKDYPYQAVDGKCDTTKVK
NKAVTIDGFEDVMAFDEMALQKAVAHQPVSVAIEASGMALQFYQSGVFTGECGSALDHGV
VIVGYGTEDGIDYWLVRNSWGRDWGENGYIKMQRNVVDTFTGKCGIAMESSYPIKNTQNP
VKISSV
>MeRDL1(cassava4.1_007020m)
MMGLFGSSAAMFVLLFLSFTLSSASDMSIISYDQTHATKSSWRTDDEVMAIYEEWLVKQG
KVYNALGEREKRFQVFKDNLRFIDEHNSENRTYKLGLNGFADLTNEEYRSTYLGARGGMK
RNRLRKTSDRYAPRVGESLPDSVDWRKEGAVAEVKDQGSCGSCWAFSTIAAVEGINKIVT
GDLISLSEQELVDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYLARDGRCDTYRK
NAKVVTIDDYEDVPVNSETALQKAVANQPVSVAIEAGGRDFQFYASGIFSGRCGTQLDHG
VAAVGYGTENGKDYWIVRNSWGKSWGENGYLRMARSINSPTGICGIAMEASYPIKKGQNP
PNPGPSPPSPVTPPTVCDNYYSCPDSNTCCCLFEYGNFCFEWGCCPLEGATCCEDHYSCC
PHDYPICNINQGTCLMSKDNPLAVKAMIRIPAKPHWALGAAAKKSSA
>CsRDL1(orange1.1g017419m)
MATASMFLAISTLVFLFFISSSSAADMSIISYDNNHDHSSSWRTDDEVMTIYQTWLAKHG
KTSNGMGHNEKRFQIFKDNLRFIDEHNSLNRTYKVGLNKFADLTNEEYRAMYLGTRSDAK
RRLMKSKVASQRYACKAGDELPESVDWREKGAVNPVKDQGSCGSCWAFSTVAAVEGINKI
VTGELISLSEQELVDCDRKINAGCNGGLMDYAFQFIIQNGGMDSEQDYPYLGAENKCDPS
RRNAKVVSIDGYEDVSPFDEMSLKKAVADQPVSVAIEAGGRAFQHYESGVFTGECGSALD
HGVVAVGYGTENGVDYWLVRNSWGSDWGENGYVKLQRNLLDTNTGKCGIAMEASYPVKNS
QNSAKPKPHSSA
>ZmRDL1(GRMZM2G166281_T01)
MAAPASTTAAAAAALLLLLLLSLAAAADMSIVSYGERSDEEARRMYAEWMAAHGRTYNAV
GEEERRYQVFRDNLRYIDAHNAAADAGVHSFRLGLNRFADLTNDEYRATYLGARTRPQRE
RKLGARYHAADNEDLPESVDWRAKGAVAEVKDQGSCGSCWAFSTIAAVEGINQIVTGDLI
SLSEQELVDCDTSYNQGCNGGLMDYAFEFIINNGGIDTEKDYPYKGTDGRCDVNRKNAKV
VTIDSYEDVPANDEKSLQKAVANQPVSVAIEAAGTAFQLYSSGIFTGSCGTALDHGVTAV
GYGTENGKDYWIVKNSWGSSWGESGYVRMERNIKASSGKCGIAVEPSYPLKEGANPPNPG
PSPPSPTPAPAVCDNYYSCPDSTTCCCIYEYGKYCFAWGCCPLEGATCCDDHYSCCPHDY
PICNVRQGTCLMGKDSPLSLSVKATKRTLAKPHWAFSGNTADGMKSSA
>BrRDL1(Bradi5g24027.1)
MRSSMALVAAAALLLLVSLAAAADMSIVSYGERSEEEVRRMYVEWMAENGRTYNAIGEEERRFEVF
RDNLRYVDQHNAAADAGLHSFRLGLNRFADLTNE
EYRDTYLGVRTKPVRERRLSGRYQAADNEELPESVDWREKGAVAKVKDQGGCGSCWAFSAIAAVEG
INQIVTGDMIALSEQELVDCDTSYNQGCNGGLMD
YAFEFIINNGGIDSEEDYPYKERDNRCDANKKNAKVVTIDGYEDVPVNSELSLKKAVANQPISVAIEAG
GRAFQLYKSGIFTGRCGTALDHGVTAVGYGS
ENGKDYWIVKNSWGTVWGEDGYVRLERNIKATSGKCGIAIEPSYPLKKGANPPNPGPTPPSPAPPSTVC
DSYNECPASTTCCCIYTYGKECFAWGCCPLE
GATCCDDHYSCCPHSYPICNVQQGTCLAGKDSPMSVKALKRILAKPHGTFSGKSSA
>GmRDL1(Glyma14g09440.1)
MFTAPFPHSLSHLSPKTSPVGSSSSITTTSSKTMTMAMATILLLFTVFAVSSALDMSIIS
YDNAHAATSRSDEELMSMYEQWLVKHGKVYNALGEKEKRFQIFKDNLRFIDDHNSQEDRT
YKLGLNRFADLTNEEYRAKYLGTKIDPNRRLGKTPSNRYAPRVGDKLPESVDWRKEGAVP
PVKDQGGCGSCWAFSAIGAVEGINKIVTGELISLSEQELVDCDTGYNEGCNGGLMDYAFE
FIINNGGIDSEEDYPYRGVDGRCDTYRKNAKVVSIDDYEDVPAYDELALKKAVANQPVSV
AIEGGGREFQLYVSGVFTGRCGTALDHGVVAVGYGTANGHDYWIVRNSWGPSWGEDGYIR
LERNLANSRSGKCGIAIEPSYPLKNGPNPPNPGPSPPSPVKPPNVCDNYYSCADSATCCC
IFEFGNACFEWGCCPLEGATCCDDHYSCCPNDYPICNTYAGTCLKSKNNPFGVKALRRTP
AKPHWTFGRKNKVSSA
>PtRDL1(Potri.007G047600.1)
MALAISTLLFLFFTVSSALDMSIFNHDDNHLSHDQSSWRSDDEVMSIYKWWLQKHGKAYN
RLGEKAKRFEIFKNNLRFIDEHNSQNRTYKVGLTKFADLTNQEYRAMFLGTRSDPKRRLM
KSKNPSERYAYKAGDKLPESVDWRGKGAVNPIKDQGSCGSCWAFSTVAAVEGINQIVTGE
LISLSEQELVDCDRFYNAGCNGGLMDYAFQFIINNGGLDTEKDYPYLGNDDTCDRDKMKT
KAVSIDGFEDVLPFDEKALQKAVAHQPVSVAIEASGMALQFYQSGVFTGECGTALDHGVV
VVGYGTEKGLDYWLVRNSWGTEWGEHGYIKMQRNVRDTYTGRCGIAMESSYPVKNGQNTA
KPYLADESAGEKISSI
>PvRDL1(Phvul.001G024500.1)
MASSTSANMAIMLLFALFALSSALDMSIISYDNAHQDKATWRTDEEVNSLYEEWLVKHGK
LYNALGEKDKRFQIFKDNLRFIDQQNAENRTYKLGLNRFADLTNEEYRARYLGTKIDPNR
RLGRTPSNRYAPRVGETLPDSVDWRKEGAVVPVKDQASCGSCWAFSAIGAVEGINKIVTG
DLISLSEQELVDCDTGYNMGCNGGLMDYAFEFIIKNGGIDSEEDYPYKGVDGRCDEYRKN
AKVVSIDGYEDVNTYDELALKKAVANQPVSVAVEGGGREFQLYSSGVFTGRCGTALDHGV
VAVGYGTDNGHDFWIVRNSWGADWGEEGYIRLERNLGNSRSGKCGIAIEPSYPIKTGQNP
PNPGPSPPSPVKPPNVCDNYYSCSDSATCCCIFEFGKTCFEWGCCPLEGATCCDDHYSCC
PHDYPICNTYAGTCLRSKNNPFGVKALRRTPAKPHGAFAGNKVSNA
>SlRDL1(Solyc01g107760.2.1)
MTYFRPLFLFLLTFFVLSSALDMSIISYDEKHADLGATNHRTDDEVKGLYESWIVKHGKN
YNAIGEKEKRFEIFKDNLRFIDEQNAETRPYKLGLNRFSDLTNDEYRALFVGGRFDKKTR
LLKNPKSERYAFKAGEKLPESVDWRQKGAVAPVKDQGQCGSCWAFSTVGAVEGINQIVTG
ELISLSEQELVDCDKSYNQGCNGGLMDYAFEFIKNNGGIDTEADYPYRAKDGTCDSNRKN
ARVVSIDGYEDVPINDEKSLMKALSNQPVSVAIEAGGRAFQHYSSGVFTGYCGTQLDHGV
VAIGYGTDNGSDYWIVRNSWGPNWGESGYIRLERNLANSTSGKCGIAMEPSYPLKNGANP
PNPGPSPPSPVAPSTVCDEYYSCTAGTTCCCIYKYGDYCFGWGCCPYESATCCDDNNSCC
PHDYPVCDVNSGTCQMSKDSPLSVKALKRGPATARVNWSGMKSNRKVSYD
>AtSCPL20(At4g12910)
MSIITMVWLMKVFVFVTLLSLVFVITESAPESALITKLPGFEGTFPSKHYSGYVTIDKEHGKNLWYYFIE
SEKNPSKDPVVLWLNGGPGCSSMDGFVYEHGPFNFELPKKNNSLPLLHLNPYSWSKVSNIIYLDSPVG
VGFSYSNNKSDYITGDIKTAVDSHAFLLKWFQMFPEFQSNPFFISGESYAGVYVPTLASEVVIGNKNGV
KPALNFKGYLVGNGVADPKFDGNAFVPFAHGMGLISDELFENVTKACKGNFYEIEGLECEEQYTKVN
DDTNQLNIYNILEPCYHGTSLSAFDIRSLPSSLLQLGKTEKRLPIRKRMFGRAWPVRAPVHPGIVPSWSQ
LLADVTVPCIDDRVATAWLNDPEIRKAIHTKEESEIGRWELCSGKLSFYHDAGSMIDFHRNLTLSGYRA
LIYSGDHDMCVPFTGSEAWTKSLGYKVIDEWRAWISNDQVAGYTQGYANNLTFLTIKGAGHTVPEYK
PREALDFYSRFLEGSKI
>SbSCPL20(Sb08g006600.1)
MARGGSTAAPPPLPPLLLLLVVVVVSSAFFARCCHAAPPGALVTSVPGYTAGSGALPSKHYAGYVTVD
EAHGRRLFYYLVESERDPAKDPVVLWLNGGPG
CSSFDGFVYEHGPFNFEAGGSAGSLPKLHLNPYSWSKVSSVIYLDSPAGVGLSYSKNVSDYETGDLKT
AADSHTFLLKWFQLYPEFLKNPFYIAGESYAG
VYVPTLSHEVVKGIHKGDKPTINFKGYMVGNGVCDTVFDGNALVPFAHGMALISESIYKEANTACQG
SYWNSSSAKCDDALSKVDTALDGLNIYDILEPC
YHGTNTKEGIPQSNKLPPSFKDLGVTSKPLPVRNRMHGRAWPLRAPVRDGRVPSWQELAASVPRGVP
CMSDEVATAWLNNDNVRSAIHAEPVSSIGPWQL
CTDKIDFDHDAGSMIIYHKNLTSQGYRALIYSGDHDMCVPHTGTEAWTASLGYGIIDSWRQWIVNEQV
AGYTQGYENGLTFTTIKGSGHTVPEYKPQESL
AFYSRWLNGTKL
>OsSCPL20(LOC_Os12g15470.1)
MARRGRRSLASPAVAIALFVFLAYGGGGGGGGVCEAAPASAVVKSVPGFDGALPSKHYAGSCRVAA
AAACRYVTVEEQHGRNLFYYLVESERDPAKDPLV
LWLNGGPGCSSFDGFVYEHGPFNFESGGSAKSLPKLHLNPYSWSKVSSVIYLDSPAGVGLSYSKNTSD
YNTGDLKTAADSHTFLLKWFQLYPEFLSNPFY
IAGESYAGVYVPTLSHEVVKGLHDGVKPTINFKGYMVGNGVCDTVFDGNALVPFAHGMALISDDIYQ
EAQTACHGNYWNTTTDKCENALYKVDTSINDLN
IYDILEPCYHSKTIKKVTPANTKLPKSFQHLGTTTKPLAVRTRMHGRAWPLRAPVRAGRVPSWQEFAR
GSRPSGVPCMSDEVATAWLNNDDVRAAIHAQP
VSSIGSWLICTNVLDFIHDAGSMISYHKNLTGQGYRAFIYSGDHDMCVPYTGTEAWTRSLGYGVIDSW
RPWHLNGQVSGYTQGYEHGLTFATIKGAGHTV
PEYKPQESLAFYSRWLAGSKL
>PtSCPL20 (Potri.014G177500.1)
MAKSCPILYRIFCMLLSFVLLTHSAPETALVTQLPGFSGTFPSKHYSGYVTIDESQGKRLFYYFVESERN
PPKDPVVLWLNGGPGCSSFDGFVYEHGPFN
FEAAETKGDLPKLHLNPYSWSKVSSVLYLDSPAGVGLSYSKNETDYITGDTKTASDSHAFLLKWFELY
PEFLSNPFFISGESYAGIYVPTLAYEVVKGLD
AGVKPILNFKGYLVGNGVTDEEFDGNALVPFAHGMGLIPDELFEEVTKECTGNFYNPLGETCESKLQK
VYKDVEGLNIYDILEPCYHGSNIREVTDDRIR
LPSSFRQLGETERPLPVRKRMFGRAWPFRAPVRPGIVPTWPQLLDGESVPCTDDEVATSWLNNEAVRK
AIHAELESVSGTWELCTDRIRFHHDAGSMIKY
HRNLTLRGFRALIFSGDHDMCVPYTGSEAWTRSMGYDIVDEWRPWTSNGQVAGYTQGYANNLTFLT
MKGAGHTVPEYKPREALDFYSRFLSGKPI
>PpSCPL20 (Pp1s200_78V6.1)
MAKLSRNFGSVWVCFMIVSAVLSVGVDGLRSNLIRLATERGNGGGEDVGRKSIADPHLIVSLPGAPKF
PFSMRSGYITVDEKAGRALFFWFVEADVQDPA
SAPLTLWLNGGPGCSSVGGGMLSELGPFYPTRDGAHLLPNAHAWNKVSNMLFLESPAGVGFSYSNTT
TDYKTGDKRTAQDSYAFLLRFFEQYPLYSSSKF
YISGESYAGHYVPQLADTILEGNKVGSNKKINLQGMLVGNAWTDANVDNFGAIFFWWTHALVSDSTF
KGVVKNCNFSSVGPLRSEADDLCDKYVDIANNE
LGNINIYEIYADICVSAQAQAETRHFGKQLSRTRFGGLSTRPLMKDSYDPCVDDEVEVYLNRPEVQEAL
HANTTHLPWRWTDCSEIVDYSFDDLLSSVLP
VYHNLLESNIKILVFSGDVDAIVPVTGTRTWLNLLPLNITEAWRPWTVDNQVGGYVTKYDKLTFSTVR
GAGHMVPYTQPARALHLFQSFINNTPL
>ZmSCPL20(GRMZM2G073908_T01)
MACSPSAPLARAVTAILLLASIICCCRSAPPGALVTRLPGFDGAKLPSKHYAGYVTVDEHVGSKLFYYL
VESERDPARDPVVLWLNGGPGCSSMDGFVYE
HGPFNFESGGSSGNLPKLHLNPYSWSKVSSVIYLDSPSGVGLSYSKNVSDYETGDLKTAADSHTFLLK
WFQLYPEFQKNPFYIAGESYAGVYIPTLANQV
VQGIHKGDNPVINFKGYMVGNGVCDVTFDGNALVPFAHGMGLISDDIYEQTNTACQGNYWNYSYSE
KCADAVSNVDMVISGLNIYNILEPCYHGTNTKEV
ISRRSNNNRMPQSFKDLGVTSRPLPVRTRMFGRAWPLRAPVRDGRVPSWQELAATNNFPSVMCMNDE
VATAWLNHDSVRSAIHAEPVSAIGPWILCTDQL
LFHHDAGSMIIYHKNLTSQGYRAIIYSGDHDMCVPYTGSLAWTTSLRYGVIDSWRPWFVNGQVSGYT
QGYENGLTFATIKGSGHAVPEYKPQEALAFYSR
WLAGSKL
>BrSCPL20 (Bra032681)
MRFLLKVLAFVTLLLVFLITTESAPQSALITKLPGFSGTFPSKHYSGYVTIDKEHGKNLWYYFVESEMN
PSKDPVVLWLNGGPGCSSMDGFVYEHGPFNF
EPAKTNYSLPLLHLNPYSWSKVSNMIYLDSPVGVGFSYSKNESDYITGDMKTAVDSHAFLLKWFQMFP
EFQSNPFYISGESYAGVYVPTLASEVVKGNKE
GLKPALNLKGYLVGNGVADSMYDGNALVPFAHGMGLVSDQLFENVTKACNGNFYDNLSPDCEEQM
AKVSMDIDRLNIYNILEPCYHGTSLSAFDIKSLPS
TLLQLGKTEKPLAVRKRMFGRAWPVRAPILPGIVPSWSQLLADVSVPCIDDRVATAWLNDPAIRKAIH
AKEESEIGRWELCTGKLWFHHDAGSMISFHRN
LTLSGYRALIYSGDHDMCVPFTGSEAWTHSLGYKVIDEWRAWISNDQVAGYTEGYANNLTFLTIKAL
NHNSLLKLEKWLPRHTVPEYKPREALDFYSRFL
AGSKI
>RcSCPL20 (rco30178.m000856)
MDKPKTTSLILCCIACFLFSFVLITHSAPQDALVIEIPGFSGALPSKHYSGYVTIDESHGKKLFYYFVESE
GNPSQDPVVLWLNGGPGCSSFDGFVYEHG
PFNFEAANASADLPKLHLNPYSWSKVSNILYLDSPAGVGLSYSKNTTDYITGDIKTALDSHTFLLKWFE
LYPEFLSNPFFIAGESYAGVYVPTLAYEVMK
GIDASVKPILNLKGYLVGNGVTDELFDGNALVPFAHGMGLISDDLYEEVKDACSDNFYNPLSDTCETK
LDKVDEDIEGLNIYDILEPCYHGTDPSEVKDI
KIRLPSSFRQLGKTDRPLPVRKRMFGRAWPLRAPVRDGIVPTWPQLLNSESVPCTDDEVATLWLNNAA
VRKAIHADEESIAGTWELCTDRIFFSHDAGSM
IKYHRNLTMRGFRALIFSGDHDMCVPYTGSQAWTRSMGYKIVDEWRPWISKGQVAGYTQGYENNLT
FLTIKGAGHTVPEYKPQEAFDFYSRFLAGKRI
>GmSCPL20 (Glyma18g47820.1)
MAMENQNFLEYWVLPLLMMLLLGHWISVQAAPSPSLITQLPGFNANFPSKHYSGYISIDGNAESGKNL
FYYFVSSESSPEKDPVVLWLNGGPGCSSFDGF
VYEHGPFNFEAANSKGNLPTLHINPYSWSKVSNIIYLDSPAGVGLSYSKNTSKYATGDLETASDTHVFL
LKWFQQFPEFQANPFYIAGESYAGVYVPTLA
FEVAKGIRSGTKPVINFKGYMVGNGVTDEIFDGNALIPFVHGMGLISDSIYENLQSSCKGNYYDAYSLD
ENDVCYKTIEKVDRAIDGLNVYNILEPCYHF
PDAATAKENGTLPRSFKQLGVTERPLPVRKRMFGRAWPFRAPVKPGLVPLWPQLAQTRHVACVSDEV
ASSWLNNVAVRKAIHAESEKVAGPWELCSSRIE
YHHNAGSMIPYHKNLTRLGYRALIFSGDHDMCVPFTGSEAWTRSLGYKIVDEWRPWNSNNQVAGYL
QAYENNLTFLTIKGAGHTVPEYKPREALDFYSRW
LEGKLI
>AtUP9 (At1g29120)
MSTASSWIQQPPFRYLPDRGGFSKPSSRSARQFSSGVVSASASSPSPSSSCSCGYSEILNFDFGSNRSWNQ
QGRRVQAMSSTAQRKFSLSKGDSDDKNEPDHLLVLVHGILASPSDWLYVEAELKRRLGRRFLIYASSS
NTFTKTFGGIDGAGKRLAEEVRQVVQKSKSLKKISFLAHSLGGLFSRHAVAVLYSAAMAQVSDVAVS
QSGNSNLLRGRIAGLEPINFITLATPHLGVRGRKQLPFLLGVPILEKLAAPIAPFFVGRTGSQLFLTDGKA
DKPPLLLRMASDGEDLKFLSALGAFRSRIIYANVSYDLDMVGWRTSSIRRETELIKPSRRSLDGYKHVV
DVEYCPPVSSDGAHFPPEAAKAKEAAQSSPSPQNTLEYHEIVEEEMIRGLQRLGWKKVDVSFHSTFWP
YLAHNNIHVKSERLYKAGAGVIAHVADSIKQQESSTFITASL
>VvUP9 (GSVIVT01021209001)
MHGKYEPDHLLVLVHGILASPSDWTYAEAELKKRLGRNFLIYVSSSNTYTKTFGGIDGAGKRLADEVT
QVVQKTQSLKRISFLAHSLGGLFARYAIAVLY
TPNTSSISMIAGLEPINFITLATPHLGVRGKKQLPFLLGVPILEKIAAPIAPIFAGRTGSQLFLTDGKPNKPP
LLLRMASNCEDGKFIAALGAFSCRILY
ANVSYDHMVGWRTSSIRRETELVKPPRRSLDGYKHVVDVEYCPPVLSDGPHFPPEAAKAKEAAQNAP
STQSTVEYHEILEEEMIHGLQQLGWKKVDVSFH
SAFWPFFAHNNIHVKNEWLYNAGVGVVAHVAESLKQQESSTFIAANL
>BrUP9 (Bra010851)
MSTASWIQPPCRYYSPDRRGGGFAKPSCSRARQFPGVVSSSSCSCGYSEILNFDFGSSRSWNHQGLRVQ
AMSATAQRKFSLSKGDADEKVEPDHLLVLVH
GILASPSDWLYVEAEMKRRLGRRFLIYASSSNTFTKTFGGIDGAGKRLAEEVRQVIQKSKSLKKISFLAH
SLGGLFARHAVAVLYSAPASDGAAVSNSGN
SHLPRGLLAGLEPINFITLATPHLGVRGRKQLPFLLGVPILERLAAPLAPFVVGRTGSQLFLTDGKADKP
PLLLRMASDCEDLKFLSSLGSFRSRIVYAN
VSYDHMVGWRTSSIRRETELFKPPRRSLDGYKHVVDVEYCPPVSSDGAHFPPEAAKAKEAAQSSPSPQ
NTLEYHEIVEDEMIRGLQTLGWKKVDVSFHST
FWPYLAHNNIHVKSERLYKAGAGVVAHVADSIKQQETSTFITASL
>GmUP9 (Glyma08g12210.1)
MATAPFIHARCAYSPRISNRNNKPSSQQGPLASSSSHASSSSSPSSSSFSHPSGTNSGQRHQGLRAQAMS
TVTLGNSIASIGNLKNDPDHLLVLVHGILA
STADWTYAEAELKRRLGKNFLIYVSSSNTYTKTFTGIDGAGKRLADEVLQVVKKTKSLKRISFLAHSLG
GLFARYAIAVLYSPDTYSRDQPGDLANSMTE
NSQGTTLSRGGMIAGLEPINFITLATPHLGVRGKKQLPFLLGVPILEKLAAPIAPFFVGQTGSQLFLTDGK
PDKPPLLLRMASDSDDGKFLSALGAFRCR
IIYANVSYDHMVGWRTSSIRRETELSKKPPRQSLDGYKHVVDVEYCPPVPSDGPKFPPKAVKAKEAAQ
NAPNTQNTVEYHEIVEDEMIRGLQQLGWKKVD
VSFHSAFWPFFAHNNIHVKNEWFHNAGVGVIAHVAESLRQLEASSFLSASL
>RcUP9(Rco29900.m001618)
MATASLIQARWCGYSPIFSNNNNFRNGAFGGPASASSSHLPSSSSCSCCSSHGLNFFSGVNSNWRHQDF
RAQAMNTAIRGNFASSKGVVNGNNEPDHLLV
LVHGILASPSDWIYVEAELKKRLGRNFLIYASACNTYTKTFSGIDGAGKRLAEEVMQIVEKTDSLKKISF
LAHSLGGLFARYAIAVLYSENALSSGQSND
VADSTVSNVEAASSARRGTIAGLDPINFVTLATPHLGVRGKKQLPFLLGIPILEKLAPPIAPIFTGRTGSQ
LFLTDGKPNKPPLLLRMASDCEDGPFLSA
LGVFRCRILYANVSYDHMVGWRTSSIRREKELVKPPQRSLDGYKHVVDVEYCPPFSSDGPHFPPEAAK
AKEAAQNEPSMANTVEYHELMEEEMIRGLQQL
GWKKVDVSFHSALWPFFAHNNIHVKNEWLHNAGAGVIAHVADSLKQQECSSSPLITASL
>PtUP9 (Potri.011G065700.1)
MATASSASLFQAARYGYSPSPRFSNNCHYSVRNGGVAVGVGSSSSASSPSSSAHHGFNFSSSGVNNNW
KPQGIKSQATNTATKGRFSSSSGAMNGKSEPD
HLLVLVHGILASPSDWTYVEAELKRRLGKNFLIYASSCNTYTKTFSGIDGAGKRLADEVMRVVQKRES
LKRISFLAHSLGGLFARHAISVLYSEIAVNTG
QSIDVAADTSLPNSNTTCSSRRGMIAGLEPINFITLATPHLGVRGRKQLPFLLGIPILEKLALPIAPIIVGRT
GSQLFLTDGKPNKPSLLLRMTSDSEDG
KFLSALGAFRCRILYANVSYDHMVGWRTSSIRREMELVTPPMRSLDGYKHVVDVEYCPPVSSDGPHFP
PEAAKAKEAAQNEPSMKNTAEYHELMEEEMIS
GLQRLGWKKVDVSFHSAFWPFFAHNNIHVKNEWFHNAGTGVISHVADSLKQQESAMYIAASL
>ZmUP9 (GRMZM2G103137_T03)
MPSLRKISFLAHSLGGLFARYVIAILHSVSVETKNAGQSSALIVPTTRGPAKSRCASGLGSIAGLRPINFIT
LATPHLGVRGRNQLPFLQGLSILEKLAA
PLAPLIVGRTGAQLFLTDGDPSKAPLLLQMASDCDDKNYISALAAFKNRVLYANVSYDHMVGWRTSS
LRREKNLIKPSHRSLDGYKHIVNVEYCSPVSSE
GPHFPSKAARAKEAAQRSPNRENTEEYHQMMEEEMLHGLQKVGWKKVDVNFHSSFWPYSAHNNIHV
KNEWLHNAGAGVIAHVADSIKQQESRPCLPANL
>SmUP9 (smo101352)
MKSCAIGSASTSKETNQARECPDHLLVLVHGILSSPSDWKYFKQELKARLGSKFYIHASSVNSYLQTLT
GIDMAGKRLADEVSEVVQKHPQLQRISFVAH
SLGGLFARYAIGILYLPLQPSKKLMNPSITGLDPVSFITLATPHLGVRGRQQLPFLMGVPVLEKIAAPIAP
FIVGRTGRQLFLTDGKASDPPLLLRMATD
CEQGQFISALRAFKLRAVYANVSYDHMVGWRTSSIRRESELSKPPRKSLEGYKHVVNVRYCPAIQSDG
PSFEDNTAGAKEAAQKQPSSIEAKEYHDILEE
EMVRGLQQVSWQKVDVSFHSAIWPFFAHNNIHVKQEWLHYEGAGVVSHVADTLKQQEHGAFLEANL
>PpUP9 (Pp1s59_53V6.1)
MKGMSSRNISEHLPAMQPRSKKGQQCDDIQPEHLLVLVHGILSSPADWEYVQKALQRRLGNKFLIHAS
AVNSFLNTLGGIDHAGRRLASEIERIVEKVPS
LKRISFLAHSLGGLFARYAVAMLYTSKDDITEDMSTLEDFESRGEEHPVLRLRREPKIAGLEAVNYITLA
SPHLGVRGKKQLPFLLGVQVLEKLAAPIAP
FVVGRTGRQLFLTDGKASDPPLLLRMASDCSEGLFISALRAFKSRVVYANVSYDHMVGWRTSSIRRES
ELSKPPRVSMDGYKHVVNVAYYPAVNSDAPSF
QQESAQGKAAAQASPSSKKADAYHDTLEEEMVRGLQQVSWRKVDVSFHSALWPFLAHNSITVKDRW
IHYEGTGVIAHVVDAFAQQEQNVFLVASL
>AtGAPC2 (At1g13440)
MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHHELKVKD
DKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMF
VVGVNEHEYKSDLDIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQKTVDGPSMKDWRGG
RAASFNIIPSSTGAAKAVGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDEIKKAIKEESEG
KMKGILGYTEDDVVSTDFVGDNRSSIFDAKAGIALSDKFVKLVSWYDNEWGYSSRVVDLIVHMSKA
>ZmGAPC2(GRMZM2G071630_T01)
MAKIKIGINGFGRIGRLVARVALQSDDVELVAVNDPFISTDYMTYMFKYDTVHGQWKHHEVKVKDSK
TLLFGEKEVAVFGCRNPEEIPWGSVGAEYVVES
TGVFTDQEKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEKEYKSDINIVSNASCTTNCLAPLAKVIND
KFGIVEGLMTTVHAITATQKTVDGPSSKDWRG
GRAASFNIIPSSTGAAKAVGKVLPVLNGKLTGMSFRVPTVDVSVVDLTVRLEKSATYDEIKAAVKAEA
EGSLKGILGYVEEDLVSTDFQGDSRSSIFDAK
AGIALNGNFVKLVSWYDNEWGYSTRVVDLIRHMNSTK
>MdGAPC2(MDP0000645828)
MDKIRQTECIRKCSDKVYASVAYGVPRPLGDRGNMHVKGLEGHRAKNLLQPKARPEGSWAPQNLKG
PKGQKVEPIQPTSQLGAGQKFKKSCRFGRIGRLV
ARVALQRDDVELVAVNDPFITTDYMTYMFKYDTVHGAWKHHELKVKDSKTLLFGEKPVAVFGIRNP
EEIPWGEAGADFVVESTGVFTDKEKAAAHIKGGA
KKVIISAPSKDAPMFVVGVNEKEYKPDIHILSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHSITATQK
TVDGPSMKDWRGGRAASFNIIPSSTGAAKA
VGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYEQIKAAIKEESEGKLKGILGYTEDDVVST
DFIGDSRSSIFDAKAGIALNDNFVKLVSWYDN
EWGYSSRVVDLIVHVASSL
>MeGAPC2(cassava4.1_011366m)
MGKVKIGINGFGRIGRLVARVALQRDDVELVAVNDPFITVEYMTYMFKYDTVHGQWKHHELKVKDD
KTLLFGEKPVTVFGVRNPEEIPWAETGAEYIVES
TGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYKPDLHIVSNASCTTNCLAPLAKVIHD
RFGIVEGLMTTVHSITATQKTVDGPSMKDWRG
GRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSVVDLTVRLEKKATYDQIKAAIKEES
EGKLKGILGYTEDDVVSTDFIGDNRSSIFDAK
AGIALNDNFVKLVAWYDNEWGYSTRVVDLIRHIASIQ
>RcGAPC2(RCO30147.m013783)
MAKIKIGINGFGRIGRLVARVALQRDDVELVAVNDPFISTEYMTYMFKYDTVHGQWKHHELKIKDDK
TLLFGEKPVTVFGVRNPEEIPWAETGAEYIVES
TGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYTPDLHIISNASCTTNCLAPLAKVIND
RFGIVEGLMTTVHSITATQKTVDGPSMKDWRG
GRAASFNIIPSSTGAAKAVGKVLPSLNGKLTGMSFRVPTVDVSVVDLTVRLEKSATYEQIKAAIKEESE
GKLKGILGYTEDDVVSTDFIGDKRSSIFDAK
AGIALNDKFVKLVSWYDNEWGYSTRVVDLIVHVASVH
>GmGAPC2(Glyma18g01330.1)
MASDKKIRIGINGFGRIGRLVARVALQRNDVELVAVNDPFITTDYMTYMFKYDTVHGQWKHFDVKVK
DSKTLLFGEKPVTVFGIRNPEEIPWGEVGADYV
VESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNEKEYKPELDIVSNASCTTNCLAPLAK
VINDRFGIVEGLMTTVHAITATQKTVDGPSNKD
WRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMSFRVPTVDVSVVDLTVRLEKPATYEQIKSAIK
EESEGKLKGILGYTEDDVVSTDFVGDNRSSIF
DAKAGISLNDNFVKLVSWYDNEWGYSSRVIDLIVHIASVA
>PtGAPC2(Potri.001G335800.1)
MAKIKIGINGFGRIGRLVARVALQRDDVELVAVNDPFITTDYMTYMFKYDTVHGQWKHGDIKVKDEK
TLLFGDKPVAVFGVRNPEEIPWGAAGADFVVES
TGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVMGVNEKEYKPDLNIVSNASCTTNCLAPLAKVIH
DKFGIVEGLMTTVHSITATQKTVDGPSMKDWRG
GRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSVVDLTVRIEKKATYDQIKAAIKEESE
TNLKGILGYTEDDVVSTDFMGDSRSSIFDAK
AGIALNDNFVKLVSWYDNEWGYSSRVIDLIIHIASVQ
>MtGAPC2(Medtr4g103920.1)
MGGAKIKIGINGFGRIGRLVARVALKRDDVELVAVNDPFITTDYMTYMFKYDSVHGQWKNDELTVKD
SKTLLFGEKPVTVFAHRNPEEIPWASTGADIIV
ESTGVFTDKDKAAAHLKGGAKKVIISAPSKDAPMFVVGVNEKEYKPEFDIISNASCTTNCLAPLAKVIN
DRFGIVEGLMTTVHSITATQKTVDGPSAKDW
RGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSVVDLTVRLEKPATYDQIKAAIKE
ESEGNLKGILGYTEDDVVSTDFIGDTRSSIFD
AKAGIALNDNFVKLVSWYDNELGYSTRVVDLIVHIAKQL
>BrGAPC2 (Bra026904)
MADKKIKIGINGFGRIGRLVARVILQRNDVELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKLKD
EKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVV
ESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNGHEYKSDLDIVSNASCTTNCLAPLAKV
INDRFGIVEGLMTTVHSITATQKTVDGPSMKDW
RGGRAASFNIIPSSTGAAKAVGKVLPQLNGKLTGMSFRVPTVDVSVVDLTVRLEKAATYDDIKKAIKE
ESEGKLKGILGYTEDDVVSTDFVGDSRSSIFD
AKAGIALSDNFVKLVSWYDNEWGYSTRVVDLIIHMSKA
>SlGAPC2(Solyc05g014470.2.1)
MANGKIKIGINGFGRIGRLVARVALQRDDVELVAVNDPFISTDYMTYMFKYDSVHGQWKHHELKVK
DEKTLLFGEKAVTVFGIRNPEDIPWGEAGADFVV
ESTGVFTDKDKAAAHLKGGAKKVVISAPSKDAPMFVVGVNENEYKPELDIVSNASCTTNCLAPLAKVI
NDRFGIVEGLMTTVHAMTATQKTVDGPSMKDW
RGGRAASFNIIPSSTGAAKAVGKVLPQLNGKLTGMAFRVPTADVSVVDLTVRLEKEASYEDIKAAIKE
ESEGKLKGILGYTEDDVVSTDFVGDSRSSIFD
AKAGIALSKNFVKVVSWYDNEWGYSSRVIDLICHMAKA
> UM10167[Ustilago maydis glyceraldehyde-3-phosphate dehydrogenase]
MSQVNIGINGFGRIGRIVFRNSVVHNTANVVAINDPFIDLEYMVYMLKYDSTHGVFNGDI
STKDGKLIVNGKSIAVFAEKDPSNIPWGQAGAHYVVESTGVFTTIDKASAHIKGGAKKVV
ISAPSADAPMYVCGVNLDAYDPKAQVVSNASCTTNCLAPLAKVIHDKFGIVEGLMTTVHA
TTATQKTVDGPSAKDWRGGRAAAANIIPSSTGAAKAVGKVIPSLNGKLTGMAFRVPTTNV
SVVDLTARLEKGASYDEIKAEVKRASENELKGILGYTEDAVVSQDFIGNSHSSIFDAAAG
ISLNNNFVKLVSWYDNEWGYSNRCLDLLVFMAQKDSAGASRL
>gi|7669492|ref|NP_002037.2| glyceraldehyde-3-phosphate dehydrogenase isoform 1 [Homo sapiens]
MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGK
LVIN
GNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHE
KY
DNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPAS
TGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYT
EHQ
VVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
>gi|74212012|dbj|BAE40174.1| unnamed protein product [Mus musculus]
MVEVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVIN
GK
PITIFQERDPTNIEWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKY
DN
SLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPAST
G
AAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTED
QVV
SCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE
Supplemental Table IX. Proteins tested for subcellular localization in this study.
PTS1/2, peroxisome targeting signal type 1 or 2. Unverified PTSs are enclosed in parentheses. YFP orientation indicates where YFP
was located relative to the coding sequence of the gene, here NY and CY refer to YFP-gene and gene-YFP fusions respectively. T, Z,
M, E and R refer to Total, Zoom, Membrane fraction proteomics experiments in this study, and studies by Eubel et al. 2008 and
Reumann et al. 2009, respectively. Proteins that were found are indicated by x, and c in case they were reported as contaminant.
Gene model Acronym Annotation PTS1/2 YFP
orientation
Localization result T Z M E R
At1g13440.1 GAPC2 Glyceraldehyde 3-phosphate dehydrogenase C2 (SKA>) NY
cytosol &
peroxisome x x x c c
At1g14810.1 Semialdehyde dehydrogenase family protein (MLL>) NY cytosol x
At1g26340.1 B5#6 Cytochrome B5 isoform A - NY cytosol x x
CY cytosol x x
At1g29090.1 Peptidase C1A papain family protein - NY cytosol x x x
CY cytosol x x x
At1g29120.5 UP9 Unknown protein ASL> NY peroxisome x
At1g32060.1 PRK Phosphoribulokinase (AKA>) NY cytosol x
At1g47128.1 RD21 Response to dehydration 21/Cysteine-type peptidase - CY cytosol x
At1g65880.1 BZO1 Benzoate-CoA ligase 1 SRL> NY peroxisome x
At1g71250.1 GDSL-motif lipase/hydrolase family protein - CY cytosol x x
At1g78380.1 GSTU19 Glutathione S-transferase tau 19 - NY mitochondria x x
CY mitochondria x x
At2g16600.1 ROC3 Peptidyl-prolyl cis-trans isomerase - CY cytosol x x
At2g21660.1 CCR2 Cold, circadian rhythm, and RNA binding 2 - NY nucleus x x x x
CY nucleus x x x x
At2g28490.1 Cupin family protein - CY cytosol x x
At2g30970.1 ASP1 Aspartate aminotransferase 1 - CY cytosol x
At2g31670.1 UP3 Unknown protein SSL> NY peroxisome x x x
At2g36640.1 ATECP63 Embryonic cell protein 63 (GKL>) NY cytosol x
At2g38380.1 PER22 Peroxidase 22 SSI> CY cytosol x x
At2g38530.1 LTP2 Lipid transfer protein 2 - CY cytosol x x
At2g42520.1 RH37 DEAD box RNA helicase - NY non-peroxisomal dots x x
CY non-peroxisomal dots x x
At2g47730.1 GSTF8 Glutathione S-transferase phi8 - CY chloroplast x x c
At3g11630.1 2-cys peroxiredoxin (RVxSL) CY non-peroxisomal dots x x c
At3g48170.1 BADH Aldehyde dehydrogenase SKL> NY peroxisome x x x
At3g48730.1 Glutamate-1-semialdehyde 2,1-aminomutase 2 SRL> NY cytosol x
At3g55290.1 SDRd Short-chain dehydrogenase/ reductase SSL> NY peroxisome x x x x
CY cytosol x x x x
At4g12910.1 Scpl20 Serine carboxypeptidase-like 20 SKI> NY peroxisome x x
At4g18360.1 GOX3 Glycolate oxidase isoform 3 AKL> NY peroxisome x
At4g20360.1 RabE1b/8D Rab GTPase homolog E1b - NY non-peroxisomal dots x x c
CY non-peroxisomal dots x x c
At4g34200.1 EDA9 Embryo sac development arrest 9 LKL> NY cytosol x x x
At4g36880.1 RDL1 Papain-like cysteine proteinase SSV> NY peroxisome x x
At4g39260.1 CCR1 RNA binding / nucleic acid binding - NY nucleus x x x x
CY nucleus x x x x
At5g16370.1 AAE5 Acyl-activating enzyme 5 SRM> NY peroxisome x x x x
At5g17920.1 ATMS1 Cobalamin-independent methionine synthase (SAK>) NY peroxisome x x x
At5g19550.1 AAT2 Aspartate aminotransferase 2 - CY non-peroxiosmal dots x x c
At5g44020.1 Acid phosphatase class B family protein (RSxTL) CY cytosol x x
At5g46290.1 KAS1 3-Ketoacyl-acyl carrier protein synthase I - NY dots x
CY dots x
At5g48230.2 ACAT2 Acetoacetyl-CoA thiolase 2 (ELL >) NY cytosol x x x
CY cytosol x x x
Supplemental Table X. Comparison of peroxisomal proteins identified from three proteomics studies.
Tissue/Material Cell culture Leaf Etiolated seedlings
Species Arabidopsis Arabidopsis Arabidopsis
Reference Eubel et al., 2008
Reumann et al., 2009 This study
AGI no Gene Name Annotation
At1g01710 ACH2 Acyl-CoA thioesterase X X X
At1g01820 PEX11c Peroxin 11 c X X
At1g04290 sT4 Small thioesterase 4 X X
At1g04710 KAT1 3-Ketoacyl-CoA thiolase 1 X X X
At1g06290 ACX3 Acyl-CoA oxidase 3 X X X
At1g06460 ACD31.2 Small heat shock protein X
At1g11840 GLX1 Glyoxylase I homolog X
At1g13440 GAPC2 Glyceraldehyde 3-phosphate dehydrogenase C2 * * X
At1g16730 UP6 Unknown protein 6 X X
At1g19570 DHAR Dehydroascorbate reductase 1 X
At1g20480 4CLP3 4-coumarate:CoA ligase 3 X
At1g20510 OPCL1 OPC-8:0 ligase 1 X X
At1g20560 AAE1 Acyl-activating enzyme 1 X X
At1g20620 CAT3 Catalase 3 X X X
At1g20630 CAT1 Catalase 1 X X X
At1g21770 ATF1 Acetyl transferase 1 X
At1g23310 GGT1 Glutamate-glyoxylate aminotransferase 1 X X X
At1g28320 DEG15 Deg/HtrA protease X
At1g29120 UP9 Unknown protein 9 X
At1g29260 PEX7 Peroxin 7 X
At1g47750 PEX11a Peroxin 11a X X X
At1g48320 st1 Small thioesterase 1 X X
At1g49350 pxPfkB PfkB-type carbohydrate kinase family protein X
At1g49670 NQR NADH:quinone reductase X X X
At1g50510 IndA Indigoidine synthase A X X X
At1g54340 ICDH NADP-dependent isocitrate dehydrogenase X X X
At1g60550 NS Naphthoate synthase X X
At1g64850 CEF Calcium binding EF-hand X
At1g65520 ECI monofunctional enoyl CoA hydratase/isomerase c X X
At1g65840 PAO4 Polyamine oxidase 4 X
At1g65880 BZO1 BENZOYLOXYGLUCOSINOLATE 1 X
At1g68010 HPR1 Hydroxypyruvate reductase 1 X X X
At1g70580 GGT2 Glutamate-glyoxylate aminotransferase 2 X X X
At1g76150 ECH2 Monofunctional enoyl-CoA hydratase 2 X X X
At1g77540 ATF2 Acetyltransferase X X
At1g78370 GSTU20 Glutathione S-Transferase tau 20 X
At2g06050 OPR3 12-oxophytodienoate reductase 3 X X X
At2g13360 SGAT1 Serine-glyoxylate aminotransferase X X
At2g22780 PMDH1 Peroxisomal NAD+-malate dehydrogenase 1 X X X
At2g26230 Uri Uricase X X X
At2g27490 CoAE Dephospho-CoA kinase X
At2g29590 st5 Small thioesterase 5 X
At2g31670 UP3 Unknown protein X X
At2g33150 KAT2 3-Ketoacyl-CoA thiolase 2 X X X
At2g35690 ACX5 Acyl-CoA oxidase 5 X X
At2g38180 GLH GDSL motif lipase/hydrolase family protein X
At2g39970 PXN/PMP38/PMP36 Peroxisomal membrane protein of 36 Kda X X X
At2g41790 PXM16 PXM16 Peptidase M16 family X X
At2g42490 CuAO Copper amine oxidase X X X
At2g42790 CSY3 Citrate synthase 3 X X X
At2g45740 PEX11d Peroxin 11d X X X
At3g01910 SO Sulfite oxidase X X X
At3g01980 SDRc Short-chain dehydrogenase/reductase c X X
At3g02360 6PGDH Phosphogluconate dehydrogenase X X X
At3g05290 PNC1 Peroxisomal adenine nucleotide carrier 1
At3g05970 LACS6 Long-chain acyl-CoA synthetase 6 X X X
At3g06810 IBR3 IBA-response 3 X X X
At3g06860 MFP2 Fatty acid multifunctional protein 2 X X X
At3g12800 SDRb Short-chain dehydrogenase/ reductase b X X X
At3g14150 HAOX1 Hydroxy-acid oxidase 1 X
At3g14415 GOX1 Glycolate oxidase 1 X X X
At3g14420 GOX2 Glycolate oxidase 2 X X X
At3g15290 HBCDH Hydroxybutyryl-CoA dehydrogenase X X X
At3g16910 AAE7 Acyl-activating enzyme 7 X X
At3g17420 GPK1 Glyoxysomal protein kinase 1
At3g21720 ICL Isocitrate lyase X
At3g24170 GR1 Glutathione reductase 1 X X
At3g27820 MDAR4 Monodehydroascorbate reductase 4 X
At3g47430 PEX11b Peroxin 11b X
At3g48140 B12D1 Senescence-associated protein/ B12D-related protein X
At3g48170 BADH Aldehyde dehydrogenase X X X
At3g51660 MIF Macrophage migration factor X
At3g51840 ACX4 Acyl-CoA oxidase 4 X X X
At3g52880 MDAR1 Monodehydroascorbate reductase 1 X X
At3g55290 SDR Short-chain dehydrogenase/ reductase X X X
At3g55640 CDC Ca2+
dependent carrier X X
At3g56460 ZnDH Zinc-binding dehydrogenase X X X
At3g56490 HIT3 Histidine triad family protein 3 X
At3g58750 CSY2 Citrate synthase 2 X X X
At3g61070 PEX11e Peroxin 11e X X X
At3g61200 st3 Small thioesterase 3 X X X
At4g00520 ACH Acyl-CoA thioesterase family protein X
At4g02340 EH3 Epoxide hydrolase 3 X X
At4g04320 MCD Malonyl-CoA decarboxylase X X
At4g04470 PMP22 Peroxisomal membrane protein of 22 kDa X
At4g05160 4CLP1 4-coumarate:CoA ligase 1 X X X
At4g05530 IBR1 Indole-3-butyric acid response 1 X X X
At4g09320 NDPK1 Nucleoside diphosphate kinase type 1 X X
At4g12910 SCPL20 Serine carboxypeptidase-like 20 X
At4g14430 IBR10 Indole-3-butyric acid response 10 X X X
At4g16210 ECHIA Monofunctional enoyl-CoA hydratase/ isomerase a X X X
At4g16566 HIT1 Histidine triad family protein 1 X X X
At4g16760 ACX1 Acyl-CoA oxidase 1 X X X
At4g18360 GOX3 Glycolate oxidase 3 X X X
At4g29010 AIM1 Abnormal inflorescence meristem 1 X X X
At4g35000 APX3 Ascorbate peroxidase 3 X X X
At4g35090 CAT2 Catalase 2 X X X
At4g36880 RDL1 Cysteine proteinase X
At4g39850 PXA1/CTS Peroxisomal ABC transporter 1/ Comatose X X X
At5g03860 MLS Malate synthase X
At5g09660 PMDH2 Peroxisomal NAD-malate dehydrogenase 2 X X X
At5g11520 ASP3 Aspartate aminotransferase X X X
At5g11910 ELT1 Esterase/lipase/thioesterase family 1 X
At5g16370 AAE5 Acyl-activating enzyme 5 X X X
At5g17920 ATMS1 Cobalamin-independent methionine synthase X X
At5g18100 CSD3 Copper/zinc superoxide dismutase 3 X X
At5g23050 AAE17 Acyl-activating enzyme 17 X X
At5g24400 6PGL 6-Phosphogluconolactonase
At5g27520 PNC2 Peroxisomal adenine nucleotide carrier 2 X
At5g27600 LACS7 Long-chain acyl-CoA synthetase 7 X X X
At5g37670 AtHsp15.7 Heat shock protein similar to 17.6 kDa class I X
At5g41210 GSTT1 Glutathione S-transferase θ isoform 1 X X X
At5g42890 SCP2 Sterol carrier protein 2 X X X
At5g43280 AtDCI Δ3,5- Δ2,4-enoyl-CoA-isomerase X X X
At5g44250 UP5 Unknown protein 5 X
At5g47040 LON2 Lon protease homolog 2 * X X
At5g47720 ACAT1.3 Acetoacetyl-CoA thiolase 1.3 X
At5g48545 HIT2 Histidine triad family protein 2 X
At5g48880 KAT5 3-Ketoacyl-CoA thiolase 5 X
At5g58220 TLP Transthyretin-like protein X X
At5g62810 PEX14 Peroxin 14 X X X
At5g63380 4CLP2 4-coumarate:CoA ligase 2 X
At5g65110 ACX2 Acyl-CoA oxidase 2 X X
At5g65400 UP7 Unknown protein 7 X
At5g65940 CHY1 3-hydroxyisobutyryl-CoA hydrolase X X X
82 104 77
* Detected but not claimed to be peroxisomal 2 1
Total 84 105 77
Supplemental Table XI. List of proteins common or exclusive to the peroxisomal proteome of leaves, cell cultures and etiolated seedlings.
AGI No. Name Annotation
Exclusively in etiolated seedling peroxisome
1 At1g29120 UP9 Unknown protein 9
2 At1g65880 BZO1 Benzoyloxyglucosinolate 1
3 At3g21720 ICL Isocitrate lyase
4 At4g12910 SCPL20 Serine carboxypeptidase-like 20
5 At4g36880 RDL1 Cysteine proteinase
6 At5g03860 MLS Malate synthase
7 At5g37670 AtHsp15.7 Heat shock protein similar to 17.6 kDa class I
8 At5g47720 ACAT1.3 Acetoacetyl-CoA thiolase 1.3
Exclusively in cell culture peroxisome
1 At1g20480 4CLP3 4-coumarate:CoA ligase 3
2 At1g28320 DEG15 Deg/HtrA protease
3 At1g29260 PEX7 Peroxin 7
4 At1g49350 pxPfkB PfkB-type carbohydrate kinase family protein
5 At1g65840 PAO4 Polyamine oxidase 4
6 At2g38180 GLH GDSL motif lipase/hydrolase family protein
7 At4g00520 ACH Acyl-CoA thioesterase family protein
8 At4g04470 PMP22 Peroxisomal membrane protein of 22 kDa
9 At5g27520 PNC2 Peroxisomal adenine nucleotide carrier 2
Exclusively in leaf peroxisome
1 At1g06460 ACD31.2 Small heat shock protein
2 At1g11840 GLX1 Glyoxylase I homolog
3 At1g19570 DHAR Dehydroascorbate reductase 1
4 At1g21770 ATF1 Acetyl transferase 1
5 At1g64850 CEF Calcium binding EF-hand
6 At1g78370 GSTU20 Glutathione S-Transferase tau 20
7 At2g27490 CoAE Dephospho-CoA kinase
8 At2g29590 sT5 Small thioesterase 5
9 At3g14150 HAOX1 Hydroxy-acid oxidase 1
10 At3g27820 MDAR4 Monodehydroascorbate reductase 4
11 At3g47430 PEX11b Peroxin 11b
12 At3g48140 B12D1 Senescence-associated protein/ B12D-related protein
13 At3g51660 MIF Macrophage migration factor
14 At3g56490 HIT3 Histidine triad family protein 3
15 At5g11910 ELT1 Esterase/lipase/thioesterase family 1
16 At5g44250 UP5 Unknown protein 5
17 At5g48545 HIT2 Histidine triad family protein 2
18 At5g48880 KAT5 3-Ketoacyl-CoA thiolase 5
19 At5g63380 4CLP2 4-coumarate:CoA ligase 2
20 At5g65400 UP7 Unknown protein 7
Common to etiolated seedling peroxisomes, cell culture peroxisomes and leaf peroxisome
1 At1g04710 KAT1 3-Ketoacyl-CoA thiolase 1
2 At1g06290 ACX3 Acyl-CoA oxidase 3
3 At1g13440 GAPC2 Glyceraldehyde 3-phosphate dehydrogenase C2
4 At1g20620 CAT3 Catalase 3
5 At1g23310 GGT1 Glutamate-glyoxylate aminotransferase 1
6 At1g47750 PEX11a Peroxin 11a
7 At1g48320 sT1 Small thioesterase 1
8 At1g49670 NQR NADH:quinone reductase
9 At1g50510 IndA Indigoidine synthase A
10 At1g54340 ICDH NADP-dependent isocitrate dehydrogenase
11 At1g68010 HPR1 Hydroxypyruvate reductase 1
12 At1g70580 GGT2 Glutamate-glyoxylate aminotransferase 2
13 At1g76150 ECH2 Monofunctional enoyl-CoA hydratase 2
14 At2g06050 OPR3 12-oxophytodienoate reductase 3
15 At2g22780 PMDH1 Peroxisomal NAD+-malate dehydrogenase 1
16 At2g26230 Uri Uricase
17 At2g33150 KAT2 3-Ketoacyl-CoA thiolase 2
18 At2g39970 PXN Peroxisomal membrane protein of 36 Kda
19 At2g42490 CuAO Copper amine oxidase
20 At2g42790 CSY3 Citrate synthase 3
21 At2g45740 PEX11d Peroxin 11d
22 At3g01910 SO Sulfite oxidase
23 At3g02360 6PGDH Phosphogluconate dehydrogenase
24 At3g05970 LACS6 Long-chain acyl-CoA synthetase 6
25 At3g06810 IBR3 IBA-response 3
26 At3g06860 MFP2 Fatty acid multifunctional protein 2
27 At3g12800 SDRb Short-chain dehydrogenase/ reductase b
28 At3g14415 GOX1 Glycolate oxidase 1
29 At3g14420 GOX2 Glycolate oxidase 2
30 At3g15290 HBCDH Hydroxybutyryl-CoA dehydrogenase
31 At3g48170 BADH Aldehyde dehydrogenase
32 At3g51840 ACX4 Acyl-CoA oxidase 4
33 At3g55290 SDRd Short-chain dehydrogenase/ reductase
34 At3g56460 ZnDH Zinc-binding dehydrogenase
35 At3g58750 CSY2 Citrate synthase 2
36 At3g61070 PEX11e Peroxin 11e
37 At3g61200 sT3 Small thioesterase 3
38 At4g05160 4CLP1 4-coumarate:CoA ligase 1
39 At4g05530 IBR1 Indole-3-butyric acid response 1
40 At4g14430 IBR10 Indole-3-butyric acid response 10
41 At4g16210 ECHIA Monofunctional enoyl-CoA hydratase/ isomerase a
42 At4g16566 HIT1 Histidine triad family protein 1
43 At4g16760 ACX1 Acyl-CoA oxidase 1
44 At4g18360 GOX3 Glycolate oxidase 3
45 At4g29010 AIM1 Abnormal inflorescence meristem 1
46 At4g35000 APX3 Ascorbate peroxidase 3
47 At4g35090 CAT2 Catalase 2
48 At4g39850 PXA1 Peroxisomal ABC transporter 1/ Comatose
49 At5g09660 PMDH2 Peroxisomal NAD-malate dehydrogenase 2
50 At5g11520 ASP3 Aspartate aminotransferase
51 At5g16370 AAE5 Acyl-activating enzyme 5
52 At5g27600 LACS7 Long-chain acyl-CoA synthetase 7
53 At5g41210 GsTT1 Glutathione S-transferase θ isoform 1
54 At5g42890 SCP2 Sterol carrier protein 2
55 At5g43280 AtDCI Δ3,5- Δ2,4-enoyl-CoA-isomerase
56 At5g47040 LON2 Lon protease homolog 2
57 At5g62810 PEX14 Peroxin 14
58 At5g65940 CHY1 3-hydroxyisobutyryl-CoA hydrolase
Common to cell culture peroxisomes & leaf peroxisomes missing in etiolated seedling peroxisome
1 At1g01820 PEX11c Peroxin 11 c
2 At1g04290 sT4 Small thioesterase 4
3 At1g20510 OPCL1 OPC-8:0 ligase 1
4 At1g20560 AAE1 Acyl-activating enzyme 1
5 At1g65520 ECI monofunctional enoyl CoA hydratase/isomerase c
6 At2g13360 SGAT1 Serine-glyoxylate aminotransferase
7 At2g35690 ACX5 Acyl-CoA oxidase 5
8 At2g41790 PXM16 PXM16 Peptidase M16 family
9 At3g16910 AAE7 Acyl-activating enzyme 7
10 At3g24170 GR1 Glutathione reductase 1
11 At3g55640 CDC Ca2+ dependent carrier
12 At3g52880 MDAR1 Monodehydroascorbate reductase 1
13 At4g02340 EH3 Epoxide hydrolase 3
14 At4g04320 MCD Malonyl-CoA decarboxylase
15 At5g23050 AAE17 Acyl-activating enzyme 17
16 At5g58220 TLP Transthyretin-like protein
Common to leaf peroxisomes and etiolated seedling peroxisomes but not in cell culture peroxisome
1 At1g16730 UP6 Unknown protein 6
2 At1g48320 sT1 Small thioesterase 1
3 At1g60550 NS Naphthoate synthase
4 At1g77540 ATF2 Acetyltransferase
5 At2g31670 UP3 Unknown protein
6 At3g01980 SDRc Short-chain dehydrogenase/reductase c
7 At4g09320 NDPK1 Nucleoside diphosphate kinase type 1
8 At5g17920 ATMS1 Cobalamin-independent methionine synthase
9 At5g18100 CSD3 Copper/zinc superoxide dismutase 3
10 At5g65110 ACX2 Acyl-CoA oxidase 2
Present in leaf peroxisomes not in etiolated seedling peroxisome
1 At1g01820 PEX11c Peroxin 11 c
2 At1g04290 sT4 Small thioesterase 4
4 At1g06460 ACD31.2 Small heat shock protein
5 At1g11840 GLX1 Glyoxylase I homolog
6 At1g19570 DHAR Dehydroascorbate reductase 1
7 At1g20510 OPCL1 OPC-8:0 ligase 1
8 At1g20560 AAE1 Acyl-activating enzyme 1
9 At1g21770 ATF1 Acetyl transferase 1
10 At1g64850 CEF Calcium binding EF-hand
11 At1g65520 ECI monofunctional enoyl CoA hydratase/isomerase c
13 At1g78370 GSTU20 Glutathione S-Transferase tau 20
14 At2g13360 SGAT1 Serine-glyoxylate aminotransferase
15 At2g27490 CoAE Dephospho-CoA kinase
16 At2g29590 sT5 Small thioesterase 5
17 At2g35690 ACX5 Acyl-CoA oxidase 5
18 At2g41790 PXM16 PXM16 Peptidase M16 family
20 At3g14150 HAOX1 Hydroxy-acid oxidase 1
21 At3g16910 AAE7 Acyl-activating enzyme 7
22 At3g24170 GR1 Glutathione reductase 1
23 At3g27820 MDAR4 Monodehydroascorbate reductase 4
24 At3g47430 PEX11b Peroxin 11b
25 At3g48140 B12D1 Senescence-associated protein/ B12D-related protein
26 At3g51660 MIF Macrophage migration factor
27 At3g52880 MDAR1 Monodehydroascorbate reductase 1
28 At3g55640 CDC Ca2+ dependent carrier
29 At3g56490 HIT3 Histidine triad family protein 3
30 At4g02340 EH3 Epoxide hydrolase 3
31 At4g04320 MCD Malonyl-CoA decarboxylase
32 At5g11910 ELT1 Esterase/lipase/thioesterase family 1
33 At5g23050 AAE17 Acyl-activating enzyme 17
34 At5g44250 UP5 Unknown protein 5
35 At5g48545 HIT2 Histidine triad family protein 2
36 At5g48880 KAT5 3-Ketoacyl-CoA thiolase 5
37 At5g58220 TLP Transthyretin-like protein
38 At5g63380 4CL2 4-coumarate:CoA ligase 2
39 At5g65400 UP7 Unknown protein 7
Present in cell culture peroxisomes not in etiolated seedling peroxisome
1 At1g01820 PEX11c Peroxin 11 c
2 At1g04290 sT4 Small thioesterase 4
3 At1g20480 4CLP3 4-coumarate:CoA ligase 3
4 At1g20510 OPCL1 OPC-8:0 ligase 1
5 At1g20560 AAE1 Acyl-activating enzyme 1
6 At1g28320 DEG15 Deg/HtrA protease
7 At1g29260 PEX7 Peroxin 7
8 At1g49350 pxPfkB PfkB-type carbohydrate kinase family protein
9 At1g65520 ECI monofunctional enoyl CoA hydratase/isomerase c
10 At1g65840 PAO4 Polyamine oxidase 4
11 At2g13360 SGAT1 Serine-glyoxylate aminotransferase
12 At2g35690 ACX5 Acyl-CoA oxidase 5
13 At2g38180 GLH GDSL motif lipase/hydrolase family protein
14 At2g41790 PXM16 PXM16 Peptidase M16 family
15 At3g16910 AAE7 Acyl-activating enzyme 7
16 At3g24170 GR1 Glutathione reductase 1
17 At3g52880 MDAR1 Monodehydroascorbate reductase 1
18 At3g55640 CDC Ca2+ dependent carrier
19 At4g00520 ACH Acyl-CoA thioesterase family protein
20 At4g02340 EH3 Epoxide hydrolase 3
21 At4g04320 MCD Malonyl-CoA decarboxylase
22 At4g04470 PMP22 Peroxisomal membrane protein of 22 kDa
23 At5g23050 AAE17 Acyl-activating enzyme 17
24 At5g27520 PNC2 Peroxisomal adenine nucleotide carrier 2
25 At5g58220 TLP Transthyretin-like protein
Supplemental Table XV. Primers used for subcellular localization.
Gene model Primer Sequence 5'-3' Direction Orientation Comments
At1g13440.1
HU5522 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCTGACAAGAAGAT For PTS1
HU5523 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGGCCTTTGACATGTG Rev PTS1
At1g14810.1
HU3237 AAAAAGCAGGCTTCGCGACGTTCACTCATCAAACC For PTS1 AdaptorF
HU3238 AGAAAGCTGGGTTCAGAGAAGCATCTCAGCGATCTG Rev PTS1 AdaptorR
At1g26340.1
HU3569 AAAAAGCAGGCTTCCCGACACTCACAAAGCTTTATTC For PTS1 AdaptorF
HU3570 AGAAAGCTGGGTTTAAGTCTTGCGAGAGAACAAG Rev PTS1 AdaptorR
HU3571 AAAAAGCAGGCTATGCCGACACTCACAAAGCTTTATTC For PTS2 AdaptorF
HU3572 AGAAAGCTGGGTGAGTCTTGCGAGAGAACAAGAC Rev PTS2 AdaptorR
At1g29090.1
HU3432 AAAAAGCAGGCTATGTGTGATTCTTTCATCAATAAC For PTS1 AdaptorF
HU3433 AGAAAGCTGGGTGGGCAACCGGATAAAAAGCATAC Rev PTS1 AdaptorR
HU3776 GAGAATGCCCAACAACATCCACATTGGCCTTGGTATTTAAC For PTS2 AdaptorF
HU3777 GTTAAATACCAAGGCCAATGTGGATGTTGTTGGGCATTCTC Rev PTS2 AdaptorR
At1g29120.5
HU3151 AAAAAGCAGGCTTCTCTACGGCTTCTTCTTGGATTC For PTS1 AdaptorF
HU3152 AGAAAGCTGGGTTTATAAGCTGGCGGTAATAAAC Rev PTS1 AdaptorR
At1g32060.1
HU3270 AAAAAGCAGGCTTCGCTGTCTCAACTATCTACTC For PTS1 AdaptorF
HU3271 AGAAAGCTGGGTTTAGGCTTTAGCTTCTGCACG Rev PTS1 AdaptorR
At1g47128.1
HU3434 AAAAAGCAGGCTATGGGGTTCCTTAAGCCAAC For PTS2 AdaptorF
HU3435 AGAAAGCTGGGTGGGCAATGTTCTTTCTGCCTTG Rev PTS2 AdaptorR
At1g65880.1
HU3272 AAAAAGCAGGCTTCGATGATTTGGCATTATGTGAAG For PTS1 AdaptorF
HU3273 AGAAAGCTGGGTTCAAAGCCGCGAAATAAAATGTC Rev PTS1 AdaptorR
At1g71250.1
HU3428 AAAAAGCAGGCTATGAACACAAACAGAAAGAAG For PTS2 AdaptorF
HU3429 AGAAAGCTGGGTGGTGAAGGAGTGTCATCTGTTGG Rev PTS2 AdaptorR
At1g78380.1
HU2203 GGGGACAAGTTTGTACAAAAAAGCAGGCTATGGCGAACGAGGTGATTCT For PTS1
HU2204 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTACTCAGGTACAAATTTCT Rev PTS1
HU2205 GGGGACCACTTTGTACAAGAAAGCTGGGTCCTCAGGTACAAATTTCT Rev PTS2 +HU2203
At2g16600.1
HU2378 AAAAAGCAGGCTATGGCAACAAACCCTAAAGTCTAC For PTS2 AdaptorF
HU2379 AGAAAGCTGGGTGAGAAATCTGACCACAATCAGCAATG Rev PTS2 AdaptorR
At2g21660.1
HU2646 AAAAAGCAGGCTATGGCGTCCGGTGATGTTGAG For PTS2 AdaptorF
HU2647 AGAAAGCTGGGTGCCATCCTCCACCACCACCGC Rev PTS2 AdaptorR
HU2648 AAAAAGCAGGCTTCGCGTCCGGTGATGTTGAGTATC For PTS1 AdaptorF
HU2649 AGAAAGCTGGGTTTACCATCCTCCACCACCAC Rev PTS1 AdaptorR
At2g28490.1
HU3438 AAAAAGCAGGCTATGGAGAAAAACAAGAGAGC For PTS2 AdaptorF
HU3439 AGAAAGCTGGGTGAACAAAGCGGTCGCTAACCATTTC Rev PTS2 AdaptorR
At2g30970.1
HU3440 AAAAAGCAGGCTATGGCTTTGGCGATGATGATC For PTS2 AdaptorF
HU3441 AGAAAGCTGGGTGAGATGACTTGGTGACTTCATG Rev PTS2 AdaptorR
At2g31670.1
HU2650 AAAAAGCAGGCTTCATATGCGCAAGAATCCGACCGC For PTS1 AdaptorF
HU2651 AGAAAGCTGGGTCTACAGACTAGAAGACTGTG Rev PTS1 AdaptorR
At2g36640.1
HU3656 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCGTCAGACAAAC For PTS1
HU3657 GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACAGCTTTCCCTTAT Rev PTS1
At2g38380.1
HU3009 AAAAAGCAGGCTATGGGGTTTTCTCCTTCATTTTC For PTS2 AdaptorF
HU3010 AGAAAGCTGGGTGGATAGAACTCACAACACCATCG Rev PTS2 AdaptorR
At2g38530.1
HU3442 AAAAAGCAGGCTATGGCTGGAGTGATGAAGTTG For PTS2 AdaptorF
HU3443 AGAAAGCTGGGTGCCTCACGGTGTTGCAGTTGGTG Rev PTS2 AdaptorR
At2g42520.1
HU3027 AAAAAGCAGGCTTCAGTGCATCATGGGCTGATGTAG For PTS1 AdaptorF
HU3028 AGAAAGCTGGGTTTAGTCCCAAGCACTTGGAGG Rev PTS1 AdaptorR
HU3029 AAAAAGCAGGCTATGAGTGCATCATGGGCTGATG For PTS2 AdaptorF
HU3030 AGAAAGCTGGGTGGTCCCAAGCACTTGGAGGTTC Rev PTS2 AdaptorR
At2g47730.1
HU3278 AAAAAGCAGGCTATGGGAGCAATTCAAGCTCGTC For PTS2 AdaptorF
HU3279 AGAAAGCTGGGTGCTGCTTCTGGAGGTCAATAAC Rev PTS2 AdaptorR
At3g11630.1
HU3559 AAAAAGCAGGCTATGGCGTCTGTTGCTTCTTC For PTS2 AdaptorF
HU3560 AGAAAGCTGGGTGAATAGCTGAGAAGTACTCTTTG Rev PTS2 AdaptorR
At3g48170.1
HU3268 AAAAAGCAGGCTTCGCGATTACGGTGCCGAGACG For PTS1 AdaptorF
HU3269 AGAAAGCTGGGTTCAGAGCTTGGAAGGAGGTTTGTAC Rev PTS1 AdaptorR
At3g48730.1
HU3274 AAAAAGCAGGCTTCGCTGCGACGCTTACTGGATC For PTS1 AdaptorF
HU3275 AGAAAGCTGGGTTTAGAGCCGACTTAGAACCTTC Rev PTS1 AdaptorR
At3g55290.1 TOPO
HU3341 CACCGCGATGAGCAATCATCAAACTCAGGT For PTS1
HU3342 TCAGAGAGATGAAAAGATAGGCACACCGGG Rev PTS1
HU3343 GAGAGATGAAAAGATAGGCACACCGGGCAA Rev PTS2 +HU3341
At4g12910.1
HU3266 AAAAAGCAGGCTTCTCAATAATCACAATGGTTTG For PTS1 AdaptorF
HU3267 AGAAAGCTGGGTTTAAATCTTGCTTCCTTCTAGAAATC Rev PTS1 AdaptorR
At4g18360.1
HU3276 AAAAAGCAGGCTTCGAGATAACAAACGTGATGGAATATG For PTS1 AdaptorF
HU3277 AGAAAGCTGGGTCTACAGCTTGGCCGAGAGGTAATG Rev PTS1 AdaptorR
At4g20360.1
HU3565 AAAAAGCAGGCTTCGCGATTTCGGCTCCAGCCGC For PTS1 AdaptorF
HU3566 AGAAAGCTGGGTTCATTCGAGGATCGTCCCAATAAC Rev PTS1 AdaptorR
HU3567 AAAAAGCAGGCTATGGCGATTTCGGCTCCAGC For PTS2 AdaptorF
HU3568 AGAAAGCTGGGTGTTCGAGGATCGTCCCAATAAC Rev PTS2 AdaptorR
At4g34200.1
HU3658 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGTCAGCCACCGCC For PTS1
HU3659 GGGGACCACTTTGTACAAGAAAGCTGGGTCCTAGAGCTTGAGGAA Rev PTS1
At4g36880.1
HU2525 AAAAAGCAGGCTTCGCTCCTTCAACAAAAGTTC For PTS1 AdaptorF
HU2526 AGAAAGCTGGGTTCAAACACTGCTGATAGTATTTC Rev PTS1 AdaptorR
At4g39260.1
HU2638 GGGGACAAGTTTGTACAAAAAAGCAGGCTATGTCTGAAGTTGAGTACCG For PTS1
HU2639 GGGGACCACTTTGTACAAGAAAGCTGGGTCTTACCAGCCGCCACCACCGC Rev PTS1
HU2640 GGGGACCACTTTGTACAAGAAAGCTGGGTCCCAGCCGCCACCACCGC Rev PTS2 +HU2638
At5g16370.1
HU3025 AAAAAGCAGGCTTCGAGCAAATGAAGCCATGCGC For PTS1 AdaptorF
HU3026 AGAAAGCTGGGTTTACATCCGACTCAACCTCGTC Rev PTS1 AdaptorR
At5g17920.1
HU1840 GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCTTCACACATTGTTGG For PTS1
HU1841 GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACTTGGCACTGGCGAG Rev PTS1
At5g19550.1
HU3430 AAAAAGCAGGCTATGGATTCCGTCTTCTCTAAC For PTS2 AdaptorF
HU3431 AGAAAGCTGGGTGGCCGAGGCGGGTCACTGCAGC Rev PTS2 AdaptorR
At5g44020.1
HU2112 AAAAAGCAGGCTATGGCTAGATCCTTGTTGCTCTC For PTS2 AdaptorF
HU2113 AGAAAGCTGGGTGGGCGACATAGTAGATGGAGTTAGG Rev PTS2 AdaptorR
At5g46290.1 TOPO
HU3371 CACCGCGATGCAAGCTCTTCAATCTTCATCT For PTS1
HU3372 TCAGGGTTTGAAGGCAGAGAAGGCGACTAC Rev PTS1
HU3373 GGGTTTGAAGGCAGAGAAGGCGACTACCGA Rev PTS2 +HU3371
At5g48230.2 TOPO
HU3191 CACCGCGATGGCCCATACATCAGA For PTS1
HU3192 TCAAAGGAGCTCAAGAAC Rev PTS1
HU3193 AAGGAGCTCAAGAACTAG Rev PTS2 +HU3191
AdaptorF GGGGACAAGTTTGTACAAAAAAGCAGGCT For -
AdaptorR GGGGACCACTTTGTACAAGAAAGCTGGG Rev -
Supplemental Table XVI. Primers used for genotyping and RT-PCR analysis.
P1 and P2 were used for RT-PCR; P3 and P4 were used for genotyping.
name sequence
LB_SALK ATTTTGCCGATTTCGGAAC
LB_SAIL TAGCATCTGAATTTCATAACCAATCTCGATACAC
At1g65880 BZO1 P1 CTCTAACCCCCATGACGTTC
At1g65880 BZO1 P2/P4 GTCCAACCGGCCGTTTAACT
At1g65880 BZO1 P3 GCAGACCGAGGCCACTGGTC
At4g12910 SCPL20 P1 ATCAGCTCCTGAATCTGCTC
At4g12910 SCPL20 P2 CTCCCGCGGTTTGTACTCAG
At4g12910 SCPL20 P3.1 CCGCGGTTGACTCTCACGCG
At4g12910 SCPL20 P4.1 ACGAACTGGCCACGCTCGAC
At4g12910 SCPL20 P3.2 GAGGGCATGGATATCAAATGA
At4g12910 SCPL20 P4.2 TGGAAATGGACCACAAAGGC
At4g18360 GOX3 P1 TGCATCTGGTGCTGAAGATC
At4g18360 GOX3 P2/P4 CTTGGCCGAGAGGTAATGAG
At4g18360 GOX3 P3 TGTCAAGGGTGTTATTACAGCT
At4g36880 RDL1 P1/P3 GCAAGGAGGTTCCAGAGAC
At4g36880 RDL1 P2/P4 CCGGATTTGGAGGCTGCC
At1g13440 GAPC2 P1 CACCACCGAGTACATGACATACATGTTT
At1g13440 GAPC2 P2 TCTTCTGAGTAGCAGTGATAGAGTGGACAG
At1g13440 GAPC2 P3 AATGGTTGGAGTAATGTTGCTG
At1g13440 GAPC2 P4 TGTCTGCAACAAATCGATACC