SUPPLEMENTAL INFORMATION A two-step transport pathway allows the mother cell to nurture the developing spore in Bacillus subtilis

Fernando H. Ramírez-Guadiana, Alexander J. Meeske, Christopher D. A. Rodrigues, Rocío del Carmen Barajas-Ornelas, Andrew C. Kruse, and David Z. Rudner This PDF file includes: Supplemental Methods Supplemental Figure Legends Supplemental Figures S1-S4 Supplemental Tables S1-S3

SUPPLEMENTAL METHODS Strain constructions

Deletion mutants from the Bacillus subtilis knock-out collection were all confirmed by PCR using an oligonucleotide primer (oKO0) within the erythromycin resistance gene and a gene-specific primer.

BDR3414 [∆spoVV ∆gerA::spec] was generated by transforming B. subtilis BDR3154 (∆spoVV) with a PCR product containing the gerA::spec mutation (amplified with oligonucleotide primers oFR5 and oFR8 and template DNA from BDR3371).

BDR3416 [ycgO::cat] was generated by transforming B. subtilis 168 with pKM77. pKM77 (ycgO::cat) is a double-crossover vector for ectopic integration into the nonessential ycgO locus (Rudner Lab stock).

BDR3430 [ycgO::Phyperspank-spoVFAB (erm)] was generated by transforming B. subtilis BDR3416 with pFR001.

BDR3432 [ycgO::Phyperspank-spoVFAB (erm) amyE::PxylA-spoVV (spec)] was generated by transforming B. subtilis BDR342 with pFR002.

BDR3449 [spoVV-gfp (spec)] was generated by direct transformation of B. subtilis 168 with an isothermal assembly product derived from 3 PCR products: 1) a PCR product containing the spoVV gene lacking its stop codon amplified with oligonucleotide primers oDR1262 and oDR1263 and B. subtilis 168 genomic DNA as template; 2) a PCR product containing gfp (mgfpmut3a) and the spec cassette amplified with oligonucleotide primers oDR1264 and oDR1265 and DNA from pWX429a as template; 3) a PCR product containing the region downstream of spoVV amplified with oligonucleotide primers oDR1266 and oDR1267 and B. subtilis 168 genomic DNA as template.

BDR3458 [∆spoIIQ::kan] was generated by two back-crosses into B. subtilis 168 with genomic DNA from BCR267 (Rodrigues et al., 2016)

BDR3465 [ycgO::PyeeK-optRBS-spoVV-gfp (erm)] was generated by transforming B. subtilis BDR3416 with pFR008.

BDR3466 [ycgO::PspoVV-optRBS-spoVV-gfp (erm)] was generated by transforming B. subtilis BDR3416 with pFR009.

BDR3468 [∆spoVV ycgO::PyeeK-optRBS-spoVV-gfp (erm)] was generated by transforming B. subtilis BDR3154 with gDNA from BDR3465.

BDR3469 [∆spoVV ycgO::PspoVV-optRBS-spoVV-gfp (erm)] was generated by transforming B. subtilis BDR3154 with gDNA from BDR3466.

BDR3471 [∆gerA::spec ∆spoVV ycgO::PyeeK-optRBS-spoVV-gfp (erm)] was generated by transforming B. subtilis BDR3414 with gDNA from BDR3465.

BDR3472 [∆spoVV ∆spoIIQ::kan ycgO::PspoVV-optRBS-spoVV-gfp (erm)] was generated by transforming B. subtilis BDR3469 with gDNA from BDR3458.

BDR3474 [∆spoIIIAH ∆spoVV::spec ycgO::PspoVV-optRBS-spoVV-gfp (erm)] was generated in two steps: first, B. subtilis BCR1117 was transformed with gDNA from BDR3466, and this intermediate strain was transformed with a PCR product containing ∆spoVV::spec (amplified with oligonucleotide primers oKO260 and oFR3 and gDNA from BDR3312).

BDR3507 [ycgO::spoVV-gfp (spec)] was generated by transforming B. subtilis BDR3416 with pFR11.

BDR3527 [∆spoVV ycgO::spoVV-gfp (spec)] was generated by transforming B. subtilis BDR3154 with gDNA from B. subtilis BDR3507.

BDR3558 [∆spoVV ycgO::cat] was generated by transforming B. subtilis BDR3154 with pKM77.

BDR3562 [∆spoVV ycgO::spoVV(N97A)-gfp (spec)] was generated by transforming B. subtilis BDR3558 with pFR017.

BDR3563 [∆spoVV ycgO::spoVV(F302A)-gfp (spec)] was generated by transforming B. subtilis BDR3558 with pFR018.

BDR3564 [∆spoVV ycgO::spoVV-gfp (Q310A) (spec)] was generated by transforming B. subtilis BDR3558 with pFR019.

BDR3615 [∆spoVV ycgO::spoVV(F141A)-gfp (spec)] was generated by transforming B. subtilis BDR3558 with pFR027.

BDR3632 [∆spoVV ycgO::spoVV(G96A)-gfp (spec)] was generated by transforming B. subtilis BDR3558 with pFR024.

BDR3646 [∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (wt) (spec)], BDR3647 [∆gerAB::erm ∆spoVV ycgO::spoVV(G96A)-gfp (spec)], BDR3648 [∆gerAB::erm ∆spoVV ycgO::spoVV(N97A)-gfp (spec)], BDR3649 [∆gerAB::erm ∆spoVV ycgO::spoVV(F141A)-gfp (spec)], BDR3650 [∆gerAB::erm ∆spoVV ycgO::spoVV(F302A)-gfp (spec)] and BDR3651 [∆gerAB::erm ∆spoVV ycgO::spoVV(Q310A)-gfp (spec)] were generated by transforming B. subtilis BDR3527, BDR3632, BDR3562, BDR3615, BDR3563 and BDR3564, respectively, with a PCR product containing ∆gerAB::erm (amplified with the oligonucleotide primers oFR1 and oFR2 and gDNA from the strain BAM786 as template).

BDR3699 (∆spoVFB::erm) and BDR3700 (∆gerAB ∆spoVFB::erm) were generated by direct transformation of B. subtilis 168 and BDR3158, respectively, with a PCR product containing the mutation ∆spoVFB::erm [amplified with the oligonucleotide primers oFR60 + oFR61 and gDNA of the strain ∆spoVFB::erm (BKE collection) as template].

Plasmid construction

pFR001 [ycgO::Phyperspank-spoVFAB (erm)] was constructed in a two-way ligation with a SpeI-SphI PCR product containing the spoVFAB operon (amplified with oligonucleotide primers oDR1247 and oDR1257 and gDNA from B. subtilis 168 as template) and pER67 cut with SpeI and SphI. pER67 [ycgO::Phyperspank (lacI) (erm)] is a double crossover vector with an IPTG-inducible promoter for ectopic integration at the ycgO locus (Rudner Lab stock).

pFR002 [amyE::PxylA-spoVV (spec)] was constructed in a two-way ligation with a SalI-BamHI PCR product containing spoVV (amplified with oligonucleotide primers oDR1250 and oDR1251 and gDNA from B. subtilis 168 as template) and pDR150 cut with SalI and BamHI. pDR150 [amyE::PxylA (xylR) (spec)] is a double crossover vector with a xylose inducible promoter for ectopic integration at the amyE locus (Rudner Lab stock).

pFR008 [ycgO::PyeeK-optRBS-spoVV-gfp (erm)] was constructed in a three-way ligation with an EcoRI-BamHI PCR product containing the SigK-responsive yeeK promoter (amplified with oligonucleotide primers oFR11 and oFR12 and gDNA from B. subtilis 168 as template) and a NheI-BamHI PCR product containing spoVV with an optimized RBS fused to the mGFPmut3a (amplified with oligonucleotide primers oFR13 and oFR15 and gDNA from BDR3449 as template) into pER61 cut with EcoRI and BamHI. pER61 (ycgO::erm) is a double-crossover vector for ectopic integration at the ycgO locus (Rudner Lab stock).

pFR009 [ycgO::PspoVV-optRBS-spoVV-gfp (erm)] was constructed in a three-way ligation with a HindIII-NheI PCR product containing the promoter of spoVV (amplified with oligonucleotide primers oFR16 and oFR17 and gDNA from B. subtilis 168 as template) and a NheI-BamHI PCR product containing spoVV with an optimized RBS fused to the mGFPmut3a reporter (amplified with oligonucleotide primers oFR13 and oFR15 and gDNA from BDR3449 as template) into pER61 between HindIII and BamHI sites.

pFR011 [ycgO::spoVV-gfp (spec)] was constructed in a two-way ligation with a SpeI-BamHI PCR product containing spoVV fused to the mGFPmut3a reporter (amplified with oligonucleotide primers oFR23 + oFR24 and gDNA from BDR3449 as template) and pKM83 cut with SpeI and BamHI. pKM83 (ycgO::spec) is a double-crossover vector for ectopic integration at the ycgO locus (Rudner Lab stock).

pFR017 [ycgO::spoVV(N97A)-gfp (spec)] was constructed by site-directed mutagenesis using oligonucleotide primers oFR32 + oFR33 and plasmid pFR011.

pFR018 [ycgO::spoVV(F302A)-gfp (spec)] was constructed by site-directed mutagenesis using oligonucleotide primer oFR34 and plasmid pFR011.

pFR019 [ycgO::spoVV(Q310A)-gfp (spec)] was constructed by site-directed mutagenesis using oligonucleotide primer oFR36 and plasmid pFR011.

pFR024 [ycgO::spoVV(G96A)-gfp (spec)] was constructed by site-directed mutagenesis using oligonucleotide primers oFR40 + oFR41 and plasmid pFR011.

pFR027 [ycgO::spoVV(F141A)-gfp (spec)] was constructed by site-directed mutagenesis using oligonucleotide primers oFR46 + oFR47 and plasmid pFR011.

SUPPLEMENTAL FIGURE LEGENDS Figure S1. Suppression of ∆spoVFA and ∆spoVFB in the absence of a functional GerA receptor. Representative phase-contrast images of the indicated strains sporulated for 30 h at 37°C in liquid DSM are shown. Sporulation efficiencies are indicated above each image. Strains lacking the B subunit of the GerA receptor (GerAB) are designated ∆gerA for clarity. Scale bar indicates 2 µm. Figure S2. Sporulation by resuspension: Suppression of ∆spoVV and ∆spoVFA in the absence of a functional GerA receptor. Representative phase-contrast images of the indicated strains sporulated by resuspensin for 30 h at 37°C. Sporulation efficiencies are indicated above each image. Strains lacking the B subunit of the GerA receptor (GerAB) are designated ∆gerA for clarity. We note that the homogenous spore populations shown in Figure 1B were achieved after spore purification (see Materials and Methods). Scale bar indicates 2 µm. Figure S3. Expression of SpoVV and the SpoVFAB synthase during vegetative results in secretion of DPA into the medium. A. Growth curve of wild-type and the strains engineered to over-express the DPA synthase in the presence and absence of SpoVV. Indicated strains were grown in minimal medium supplemented with 33 mM xylose. When the cultures reached an OD600 of 0.3, IPTG was added (0.5 mM, final concentration) to induce expression of SpoVFA and SpoVFB. Samples were collected before and after IPTG addition at the indicated times to assay DPA levels in the medium. B. Representative phase-contrast and fluorescence microscopy images of the indicated strains collected 3 h after the addition of IPTG. Membranes were stained with TMA-DPH (false-colored red) and membrane permeability was assessed with propidium iodide (PI) (false-colored blue). The number of PI-positive cells was quantified for each strain. Scale bar indicates 2 µm. Figure S4. Conservation of the DPA transport pathway in endospore-forming bacteria. Phylogenetic tree showing the co-occurrence of the DPA synthases (SpoVFA and SpoVFB) and transporters (SpoVV and SpoVAA-AF) in a diverse set of 1,773 bacterial taxa. The amino acid sequences of B. subtilis SpoVFA, SpoVFB, SpoVV, and the SpoVA proteins served as queries in a BLASTp search against the NCBI ‘nr’ database with an e-value cutoff of 1x10-4. This analysis was performed through the Harvard Medical School Research Computing Orchestra cluster. The phylogenetic tree was constructed in PhyloT (http://phylot.biobyte.de/) and the BLASTp search results were plotted against the tree. The tree was visualized and annotated using the Interactive Tree Of Life web-based tool (iTOL, v3; http://itol.embl.de).

Ramírez-Guadiana, et al., Fig. S1

wt (100%)

∆spoVFB (0.0012%)

∆spoVFA (0.0017%)

∆gerA (86.1%)

∆spoVFB ∆gerA (0.196%)

∆spoVFA ∆gerA (0.207%)

wt (100%)

∆spoVFA (0.004%)

∆spoVV (0.00008%)

∆gerA (13.9%)

∆spoVFA ∆gerA (3.83%)

∆spoVV ∆gerA (2.96%)

Ramírez-Guadiana, et al., Fig. S2

Ramírez-Guadiana, et al., Fig. S3

wtP -VFABIPTG

P -VFAB + P -VVxylA

IPTG

0.1

1

10

0.010 2 4 6 8

time (h)

OD 6

00

A

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3h

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B wt

phas

em

embr

ane

PI

mem

bran

e +

PI

PI-positive cells: 0.9% 0.9%n > 2,100 n > 1,500

0.8%n > 1,700

P -VFABIPTG P -VFAB + P -VVxylAIPTG

Cor

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Methanoculleus marisnigri JR1

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Thermosipho melanesiensis BI429

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Geobacillus sp. C56-T3

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Verrucomicrobium spinosum DSM 4136

Sphaerobacter thermophilus DSM 20745

Flav

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101

Pirellula staleyi DSM 6068

Helicobacter bizzozeronii CIII-1

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233

44

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iella

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31

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Bdellovibrio exovorus JSS

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Aquifex aeolicus VF5

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Myc

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Halovivax ruber XH-70

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Thermococcus sibiricus MM 739

Lactobacillus helveticus DPC 4571

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Coryn

ebac

terium

aurim

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75

Fluv

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ffens

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SM

168

23

Haloferax mediterranei ATCC 33500

Ramlib

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Enterococcus mundtii QU 25

Herbaspirillum seropedicae SmR1

Bru

cella

abo

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41

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Mica

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Thermincola potens JRG

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Pre

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39

Thauera sp. MZ1T

Aminomonas paucivorans DSM 12260

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choc

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3

Bra

dyrh

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ium

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1

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Desulfovibrio sp. FW1012B

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9

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CC 7

421

Methanothermobacter thermautotrophicus str. Delta H

Wol

bach

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Desulfotomaculum acetoxidans DSM 771

Pseu

domon

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Hal

othi

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Deinococcus geothermalis DSM 11300

Streptococcus pseudopneumoniae IS7493

Kine

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cus

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otol

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s SR

S302

16

Desulfovibrio piezophilus C1TLV30

Erwinia billingiae Eb661

Clostridium lentocellum DSM 5427

Acine

toba

cter l

woffii

Geobacillus sp. Y412MC52

Rhodo

cocc

us fa

scian

s D18

8

Rho

dops

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mon

as p

alus

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9

Borre

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7

Psyc

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Aggr

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ter a

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com

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Bacillus thuringiensis serovar konkukian str. 97-27

Myc

opla

sma

arth

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1

Bisg

aard

Tax

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6

Coprococcus sp. ART55/1

Arth

roba

cter a

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cens

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Myc

opla

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gallis

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um s

tr. R

(low)

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lia af

zelii

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Thermotoga naphthophila RKU-10

Neisseria meningitidis MC58

Staphylococcus pseudintermedius HKU10-03

Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Anaeromyxobacter sp. Fw109-5

Desulfomicrobium baculatum DSM 4028

Ric

ketts

ia s

lova

ca 1

3-B

Chr

omoh

alob

acte

r sal

exig

ens

DSM

304

3

Oscillibacter valericigenes Sjm18-20

Herminiimonas arsenicoxydans

Bra

dyrh

izob

ium

sp.

BTA

i1

Syntrophobacter fumaroxidans MPOB

Filifactor alocis ATCC 35896

Thio

flavi

cocc

us m

obilis

832

1

Cya

noth

ece

sp. P

CC

742

4

Glu

cono

bact

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xyda

ns 6

21H

Clostridium cellulolyticum H10

Vibr

io p

arah

aem

olyt

icus

RIM

D 22

1063

3

Dehalococcoides sp. VS

Lactobacillus johnsonii NCC 533

Met

hylo

bact

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m n

odul

ans

OR

S 2

060

Desulfobacter postgatei 2ac9

Thermococcus barophilus MP

Desulfovibrio desulfuricans ND132

Kang

iella

kor

eens

is D

SM 1

6069

Myc

opla

sma

syno

viae

53

Pelo

dict

yon

phae

ocla

thra

tifor

me

BU-1

Coryn

ebac

terium

mar

is DSM 45

190

Aminobacterium colombiense DSM 12261Fl

avob

acte

riace

ae b

acte

rium

351

9-10

Chl

orob

ium

pha

eoba

cter

oide

s D

SM 2

66

Sebaldella termitidis ATCC 33386

Lactobacillus buchneri NRRL B-30929

Methanosaeta concilii GP6

Candidatus Kinetoplastibacterium desouzaii TCC079E

Enterobacter sp. 638

Legi

onel

la lo

ngbe

acha

e N

SW15

0

Streptococcus macedonicus ACA-DC 198

Desulfotomaculum ruminis DSM 2154

Desulfobacula toluolica Tol2

Mic

rolu

natu

s ph

osph

ovor

us N

M-1

Desulfobacca acetoxidans DSM 11109

Thermogladius cellulolyticus 1633

Geobacter sp. M18

Aero

mon

as s

alm

onic

ida

subs

p. s

alm

onic

ida

A449

Pediococcus claussenii ATCC BAA-344

Paenibacillus sp. Y412MC10

Clostridium chauvoei

Hal

omon

as s

p. Z

M3

Coryn

ebac

terium

ulce

rans

BR-A

D22

Deinococcus proteolyticus MRPRoseiflexus sp. RS-1

Aeropyrum pernix K1

Haem

ophi

lus

ducr

eyi 3

5000

HP

Halomicrobium mukohataei DSM 12286

Bac

tero

ides

hel

coge

nes

P 36

-108

Clostridium phytofermentans ISDg

Pyrobaculum calidifontis JCM 11548

Chr

ooco

ccid

iops

is th

erm

alis

PC

C 7

203

alph

a pr

oteo

bact

eriu

m H

IMB5

9

Clostridium pasteurianum BC1

Stre

ptom

yces

hyg

rosc

opicu

s su

bsp.

jingg

ange

nsis

5008

Syne

choc

occu

s sp

. PC

C 7

002

Streptococcus salivarius CCHSS3

Lepto

spira

borgp

eterse

nii se

rovar

Hardjo-

bovis

str. L

550

Neo

ricke

ttsia

sen

nets

u st

r. M

iyay

ama

Myc

opla

sma

cyno

s C1

42

Alkaliphilus metalliredigens QYMF

Roth

ia m

ucila

ginos

a DY

-18

[Clostridium] sticklandii

Citrobacte

r kose

ri ATCC BAA-895

Pyrobaculum oguniense TE7

Sulfi

toba

cter

sp.

DFL

14

Agro

bact

eriu

m ra

diob

acte

r K84

Hydrogenobacter thermophilus TK-6

Fran

kia

sp. E

uI1c

Halobacillus halophilus DSM 2266

Bac

tero

ides

sal

anitr

onis

DS

M 1

8170

Listeria innocua Clip11262

Anab

aena

var

iabi

lis A

TCC

294

13

Pseu

dom

onas

stut

zeri A

1501

Acetohalobium arabaticu

m DSM 5501

Eubacterium eligens ATCC 27750

Vibr

io s

plen

didu

s LG

P32

Xant

hom

onas

hor

toru

m p

v. ca

rota

e st

r. M

081

Ther

mob

ifida

fusc

a YX

Pyrobaculum neutrophilum V24Sta

Mar

inom

onas

sp.

MW

YL1

Ros

eoba

cter

den

itrifi

cans

OC

h 11

4

Can

dida

tus

Sul

cia

mue

lleri

GW

SS

Thio

cyst

is v

iola

scen

s D

SM 1

98

Desulfomonile tiedjei DSM 6799

Xylan

imon

as ce

llulos

ilytic

a DS

M 1

5894

Pseu

dono

card

ia d

ioxa

nivo

rans

CB1

190

Delftia

acido

vora

ns S

PH-1

Bacillus cellulosilyticus DSM 2522

Coraliomargarita akajimensis DSM 45221

Burk

holde

ria la

ta Cupria

vidus

nec

ator

N-1

Mur

icau

da ru

estri

ngen

sis

DSM

132

58

Pantoea vagans C

9-1

Emtic

icia

olig

otro

phic

a D

SM 1

7448

Macrococcus caseolyticus JCSC5402

Shigella sonnei Ss046

Aero

mic

robi

um m

arin

um D

SM 1

5272

Ric

ketts

ia m

assi

liae

MTU

5

Jann

asch

ia s

p. C

CS1

Micr

ococ

cus l

uteu

s NCT

C 26

65

Megamonas hyp

ermegale ART12/1

Xant

hom

onas

cam

pest

ris p

v. ca

mpe

stris

str.

ATC

C 33

913

Chlamydia muridarum str. Nigg

Moorella thermoacetica ATCC 39073

Halanaerobium praevalens DSM 2228

Pseu

domon

as flu

ores

cens

Pf0-

1

Solit

alea

can

aden

sis

DSM

340

3

Borre

lia tu

ricata

e 9.1E

+136

Chi

tinop

haga

pin

ensi

s D

SM 2

588

Rob

igin

itale

a bi

form

ata

HTC

C25

01

Wee

ksel

la v

irosa

DSM

169

22

Clostridium ljungdahlii DSM 13528

Can

dida

tus

Mid

ichl

oria

mito

chon

drii

IricV

A

Caul

obac

ter c

resc

entu

s NA

1000

Wol

bach

ia e

ndos

ymbi

ont o

f Dro

soph

ila m

elan

ogas

ter

Bellie

lla b

altic

a D

SM 1

5883

Opitutus terrae PB90-1

Thermotoga petrophila RKU-1

Ric

ketts

ia b

ellii

RM

L369

-C

Sulfurovum sp. NBC37-1

Rho

doth

erm

us m

arin

us D

SM 4

252

Parv

ularc

ula b

erm

uden

sis H

TCC2

503

Desulfurivibrio alkaliphilus AHT2

Zuno

ngw

angi

a pr

ofun

da S

M-A

87

Desulfovibrio africanus str. Walvis Bay

Cya

noth

ece

sp. P

CC

880

1

Actin

obac

illus

succ

inog

enes

130

Z

Yersinia ruckeri

Shewan

ella f

rigidi

marina

NCIM

B 400

Exiguobacterium sp. AT1b

Can

dida

tus

Hod

gkin

ia c

icad

icol

a D

sem

Met

hylo

bact

eriu

m s

p. 1

6893

Haloterrigena turkmenica DSM 5511

Candidatus Cloacamonas acidaminovorans str. Evry

Marino

bacte

r aqu

aeole

i VT8

Methanopyrus kandleri AV19

Methanobrevibacter smithii ATCC 35061

Helicobacter mustelae 12198

Mycob

acter

ium yo

ngon

ense

05-13

90

Bacillus weihenstephanensis KBAB4

Staphylococcus epidermidis ATCC 12228

Listeria monocytogenes EGD-e

Marinitoga piezophila KA3

Novo

sphi

ngob

ium

pen

taro

mat

ivora

ns U

S6-1

Xant

hom

onas

cam

pest

ris p

v. ve

sicat

oria

str.

85-

10

Pre

vote

lla m

elan

inog

enic

a AT

CC

258

45

Bra

dyrh

izob

iace

ae b

acte

rium

SG

-6C

Hirs

chia

bal

tica

ATC

C 4

9814

Caldivirga maquilingensis IC-167

Och

roba

ctru

m a

nthr

opi A

TCC

491

88

Nanoarchaeum equitans Kin4-M

Thermovibrio ammonificans HB-1

Psych

romon

as in

grah

amii 3

7

Lactobacillus rennini

Jone

sia d

enitr

ifican

s DS

M 2

0603

Terriglobus roseus DSM 18391

Escherichia coli O157:H7 str. S

akai

Borre

lia du

ttonii

Ly

Pseu

dom

onas

syrin

gae

pv. s

yring

ae B

728a

Candidatus Nasuia deltocephalinicola str. NAS-ALF

Sacc

haro

mon

ospo

ra m

arin

a XM

U15

Myc

opla

sma

agal

actia

e PG

2

Plan

obis

pora

rose

a

Enterococcus sp. 7L76

Shewan

ella a

mazon

ensis

SB2B

Blas

toco

ccus

sax

obsi

dens

DD

2

Mic

roco

leus

sp.

PC

C 7

113

Bar

tone

lla v

inso

nii s

ubsp

. ber

khof

fii s

tr. W

inni

e

Mycob

acter

ium sp

. JLS

Candidatus Saccharimonas aalborgensis

Mycob

acter

ium tu

bercu

losis

H37Rv

Arca

noba

cter

ium

hae

mol

ytic

um D

SM 2

0595

Met

hylo

mic

robi

um a

lbum

BG

8

Desulfovibrio desulfuricans subsp. desulfuricans str. ATCC 27774

Tere

dini

bact

er tu

rner

ae T

7901

Myxococcus xanthus DK 1622

Stre

ptom

yces

fulvi

ssim

us D

SM 4

0593

Desulfobulbus propionicus DSM 2032

Desulfovibrio alaskensis G20

Coryn

ebac

terium

arge

ntora

tense

DSM 44

202

Ilum

atob

acte

r coc

cine

us Y

M16

-304

Proc

hlor

ococ

cus

mar

inus

sub

sp. p

asto

ris s

tr. C

CMP1

986

Bacillus toyonensis BCT-7112

Aromatoleum aromaticum EbN1

Can

dida

tus

Amoe

boph

ilus

asia

ticus

5a2

Candidatus Tremblaya princeps PCIT

Novo

sphi

ngob

ium

sp.

PP1

Y

Candidatus Arthromitus sp. SFB-rat-Yit

Glaciec

ola ps

ychr

ophil

a 170

Salin

ispo

ra a

reni

cola

CN

S-20

5

Myc

opla

sma

suis

str.

Illino

is

Methanoregula formicica SMSP

Methanosaeta thermophila PT

Escherichia coli O83:H1 str. N

RG 857C

Rhod

anob

acte

r sp.

2AP

BS1

Methylotenera mobilis JLW8

Desulfovibrio magneticus RS-1

Neisseria gonorrhoeae FA 1090

Advenella kashmirensis WT001

Enterobacter aerogenes KCTC 2190

Streptococcus oligofermentans AS 1.3089

Trep

onem

a pr

imitia

ZAS

-2

Tsuk

amur

ella

paur

omet

abola

DSM

201

62

Fervidicoccus fontis Kam940

Clostridium botulinum A str. ATCC 3502

Psyc

hrob

acte

r sp.

PRw

f-1

Fervidobacterium pennivorans DSM 9078

Xant

hom

onas

fusc

ans

subs

p. fu

scan

s

Acidov

orax

citru

lli AAC00

-1

Adle

rcre

utzi

a eq

uolif

acie

ns D

SM 1

9450

Caldithrix abyssi DSM 13497

Anaeromyxobacter sp. K

Anaeromyxobacter dehalogenans 2CP-C

Bacillus coagulans 42406

Desulfosporosinus orientis DSM 765

Sapr

ospi

ra g

rand

is s

tr. L

ewin

Cya

noba

cter

ium

apo

ninu

m P

CC

106

05

Cellu

lomon

as fim

i ATC

C 48

4

Oceanithermus profundus DSM 14977

Shewan

ella w

oody

i ATCC 51

908

Geobacillus thermoglucosidasius C56-YS93

Candid

atus M

orane

lla en

dobia

PCIT

Vibr

io s

p. E

x25

Trep

onem

a de

ntico

la AT

CC 3

5405

Para

cocc

us h

aeun

daen

sis

Acine

toba

cter p

ittii

Baum

anni

a ci

cade

llinic

ola

str.

Hc

(Hom

alod

isca

coa

gula

ta)

Methanocaldococcus vulcanius M7

Spiro

chae

ta sm

arag

dinae

DSM

1129

3

Caldicellulosiru

ptor kristj

anssonii I7

7R1B

Arth

rosp

ira p

late

nsis

NIE

S-39

Myc

opla

sma

mob

ile 1

63K

Chlamydophila felis Fe/C-56

Cry

ptob

acte

rium

cur

tum

DSM

156

41

Bar

tone

lla h

ense

lae

str.

Hou

ston

-1

Caldicellulosiru

ptor lacto

aceticus 6

A

Desulfurobacterium thermolithotrophum DSM 11699

Sphi

ngob

acte

rium

sp.

21

Anaero

coccu

s prev

otii D

SM 2054

8

Comam

onas

sp. 7

D-2

Myc

opla

sma

ferm

enta

ns J

ER

Ignicoccus hospitalis KIN4/I

Serratia proteamaculans 568

Halopiger xanaduensis SH-6

Methanosarcina mazei Go1

Thermus oshimai JL-2

Caldicellulosiru

ptor besci

i DSM 6725

Bru

cella

can

is A

TCC

233

65

Chlamydia psittaci 02DC15

Syntrophothermus lipocalidus DSM 12680

uncultured bacterium HHV35

Mycob

acter

ium ka

nsas

ii ATCC 12

478

Clostridium tetani E88

Prop

ioni

bact

eriu

m a

vidu

m 4

4067

Myc

opla

sma

cotte

wii

Nitrosomonas sp. AL212

Cal

othr

ix s

p. P

CC

750

7

Ruminococcus champanellensis 18P13

Bifid

obac

teriu

m d

entiu

m B

d1

Nak

amur

ella

mul

tipar

tita

DSM

442

33

Salinarchaeum sp. Harcht-Bsk1

Janthinobacterium sp. Marseille

Flav

obac

teriu

m p

sych

roph

ilum

JIP

02/8

6

Dickeya dadantii E

ch703

Nautilia profundicola AmH

Phae

obac

ter g

alla

ecie

nsis

2.1

Acid

ithio

baci

llus

ferro

oxid

ans

ATC

C 5

3993

Dehalococcoides ethenogenes 195

Sacc

haro

mon

ospo

ra a

zure

a NA

-128

Glu

cona

ceto

bact

er x

ylinu

s NB

RC 3

288

Ric

ketts

ia c

anad

ensi

s st

r. M

cKie

l

Ruminococcus bromii L2-63

Pseu

domon

as pu

tida K

T244

0

Myc

opla

sma

conj

unct

ivae

HRC/

581

Enterococcus durans

Sodali

s glos

sinidi

us st

r. 'mors

itans

'

Ric

ketts

ia h

elve

tica

C9P

9

Methanomethylovorans hollandica DSM 15978

Bacillus amyloliquefaciens FZB42

Thermoanaerobacte

rium xy

lanolyticum LX-11

Shewan

ella p

utrefa

ciens

CN-32

Pseu

domon

as sy

ringa

e pv

. tom

ato

str. D

C3000

Bacillus subtilis subsp. subtilis str. 168

Thermococcus litoralis DSM 5473

Mycob

acter

ium sp

. KMS

Aste

r yell

ows w

itche

s'-br

oom

phy

topla

sma A

YWB

Stre

ptom

yces

rose

ochr

omog

enes

sub

sp. o

scita

ns D

S 12

.976

Burk

holde

ria p

hyto

firm

ans P

sJN

Acetobacterium woodii DSM 1030

Klebsiella oxyt

oca KCTC 1686

Coryn

ebac

terium

halot

olera

ns Y

IM 70

093 =

DSM 44

683

Edwardsiella ict

aluri 93-146

Arth

roba

cter s

p. F

B24

Glo

eoba

cter

kila

ueen

sis J

S1

Cand

idatu

s Phy

topla

sma

solan

i

Acine

toba

cter n

osoc

omial

is

Candidatus Kinetoplastibacterium galatii TCC219

Deinococcus peraridilitoris DSM 19664

Haem

ophi

lus

para

suis

SH01

65

Shewan

ella s

p. MR-7

Thermococcus onnurineus NA1

Azoarcus sp. BH72

Noc

ardi

opsi

s da

sson

ville

i sub

sp. d

asso

nville

i DSM

431

11

Hippea maritima DSM 10411

Thio

alka

livib

rio s

p. K

90m

ix

Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150

Staphylococcus aureus subsp. aureus NCTC 8325

Thio

mic

rosp

ira c

runo

gena

XC

L-2

Lysinibacillus sphaericus C3-41

Glaciec

ola sp

. 4H-3-

7+YE-5

Colwell

ia ps

ychr

eryth

raea

34H

Stre

ptom

yces

vio

lace

usni

ger T

u 41

13

Mycob

acter

ium rh

odes

iae N

BB3

Methanosphaerula palustris E1-9c

Campylobacter concisus 13826

Mycob

acter

ium gi

lvum Spy

r1

Deferribacter desulfuricans SSM1

Erwinia sp. Ejp617

Proteus mira

bilis HI4320

Stigmatella aurantiaca DW4/3-1

Verm

ineph

robac

ter ei

senia

e EF0

1-2

Acidov

orax

sp. J

S42

Bifid

obac

teriu

m th

erm

ophi

lum

RBL

67

Deinococcus gobiensis I-0

Halothermothrix

orenii H 168

Spha

eroc

haet

a glo

bosa

str.

Budd

y

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Bru

cella

cet

i TE

1075

9-12

Helicobacter cetorum MIT 99-5656

Geobacillus thermodenitrificans NG80-2

Actin

osyn

nem

a m

irum

DSM

438

27

Synergistetes bacterium SGP1

Yersinia pseudotuberculosis IP 32953

Halalkalicoccus jeotgali B3

Serratia symbiotica str. 'Cinara cedri'

Star

keya

nov

ella

DSM

506

Thermotoga sp. RQ2

Coryn

ebac

terium

pseu

dotub

ercu

losis

FRC41

Thermodesulfobacterium geofontis OPF15

Shewan

ella l

oihica

PV-4

Staphylococcus carnosus subsp. carnosus TM300

Helicobacter felis ATCC 49179

Thermosipho africanus TCF52B

Ralston

ia pic

kettii

12J

Bifid

obac

teriu

m lo

ngum

NC

C27

05

Enterococcus casseliflavus EC20

Gemmatimonas aurantiaca T-27

Serratia liquefaciens ATCC 27592

Cro

ceib

acte

r atla

ntic

us H

TCC

2559

Nitr

osoc

occu

s oc

eani

ATC

C 1

9707

Spiro

chae

ta sp

. L21

-RPu

l-D2

Gal

libac

teriu

m a

natis

UM

N17

9

Anaerobaculum mobile DSM 13181

Candidatus Nitrosopumilus koreensis AR1

Candidatus Hamiltonella defensa 5AT (Acyrthosiphon pisum)

Pyrococcus abyssi GE5

Mar

inom

onas

med

iterra

nea

MM

B-1

Thermotoga lettingae TMO

Arcobacter butzleri RM4018

Thermus thermophilus HB27

Por

phyr

omon

as g

ingi

valis

W83

Parv

ibac

ulum

lava

men

tivor

ans

DS-

1

Ric

ketts

ia h

eilo

ngjia

ngen

sis

54

Butyrivibrio proteoclasticus B316

Streptococcus sanguinis SK36

[Eubac

terium

] cylin

droide

s T2-8

7

Desulfococcus oleovorans Hxd3

Bacillus sp. 1NLA3E

Lead

bette

rella

bys

soph

ila D

SM 1

7132

Thermoplasma volcanium GSS1

Methylotenera versatilis 301

Desulfarculus baarsii DSM 2075

Candidatus Tremblaya phenacola PAVE

Ric

ketts

ia rh

ipic

epha

li st

r. 3-

7-fe

mal

e6-C

WPP

Lactobacillus fermentum IFO 3956

Shewan

ella b

altica

OS22

3

Spiro

plasm

a ta

iwan

ense

CT-

1

Thermaerobacter marianensis DSM 12885

Paenibacillus polymyxa E681

Caldicellulosiru

ptor sacch

arolyticus D

SM 8903

Bdellovibrio bacteriovorus str. Tiberius

Ferroplasma acidarmanus fer1

Pseu

domon

as m

igulae

Salin

ispo

ra tr

opic

a C

NB-

440

Anoxybacillus flavithermus WK1

Met

hylo

cella

silv

estri

s BL

2

Mesotoga prima MesG1.Ag.4.2

Wolinella succinogenes DSM 1740

Streptococcus pyogenes M1 GAS

Halorhabdus utahensis DSM 12940

Pyrobaculum islandicum DSM 4184

Pal

udib

acte

r pro

pion

icig

enes

WB

4

Oenococcus oeni PSU-1

Desulfovibrio gigas DSM 1382 = ATCC 19364

Sphi

ngob

ium

chl

orop

heno

licum

L-1

Rhod

ospi

rillu

m ru

brum

ATC

C 11

170

Thermoanaerobacter mathranii subsp. m

athranii str. A3

Thermus scotoductus SA-01

Ric

ketts

ia p

hilip

ii st

r. 36

4D

Dickeya dianthico

la NCPPB 453

Rahnella aquatilis CIP 78.65 = ATCC 33071

Candidatus Chloracidobacterium thermophilum B

Bac

tero

ides

vul

gatu

s AT

CC

848

2

Streptococcus pneumoniae TIGR4

Methanospirillum hungatei JF-1

Coryn

ebac

terium

jeike

ium K

411

Streptococcus lutetiensis 33

Prop

ioni

bact

eriu

m p

ropi

onic

um F

0230

a

Pleu

roca

psa

sp. P

CC 7

327

Vibr

io s

p. E

JY3

Methanosphaera stadtmanae DSM 3091

Deinococcus maricopensis DSM 21211

Hyp

hom

icro

bium

nitr

ativ

oran

s N

L23

Fran

cise

lla s

p. T

X077

308

Escherichia fergusonii ATCC 35469

Thermoproteus tenax Kra 1

Geobacter daltonii FRC-32

Chromobacterium violaceum ATCC 12472

Citrobacte

r rodentiu

m ICC168

Candidatus Solibacter usitatus Ellin6076

Ols

enel

la u

li D

SM 7

084

Hal

isco

men

obac

ter h

ydro

ssis

DSM

110

0

Clostridium autoethanogenum DSM 10061

Algo

ripha

gus

sp. P

R1

Clostridium perfringens str. 13

Hah

ella

che

juen

sis

KCTC

239

6

Carnobacterium sp. 17-4

Thermosediminibacte

r oceani D

SM 16646

Borre

lia bu

rgdo

rferi B

31

Comam

onad

acea

e bac

terium

CR

Ric

ketts

ia a

ustra

lis s

tr. C

utla

ck

Slac

kia

helio

trini

redu

cens

DSM

204

76

Haliangium ochraceum DSM 14365

Desulfotomaculum gibsoniae DSM 7213

Megasphaera elsd

enii DSM 20460

Haem

ophi

lus

para

influ

enza

e T3

T1

Haloquadratum walsbyi DSM 16790

Burk

holde

ria sp

. CCG

E100

1

Collimonas fungivorans Ter331

Flav

obac

teriu

m in

dicu

m G

PTSA

100-

9

Ruminococcus sp. SR1/5

Egge

rthel

la s

p. Y

Y791

8

Azot

obac

ter v

inelan

dii D

J

Pre

vote

lla d

entic

ola

F028

9

Bra

dyrh

izob

ium

olig

otro

phic

um S

58

Desulfurococcus fermentans DSM 16532

Par

abac

tero

ides

dis

taso

nis

ATC

C 8

503

Sacc

haro

thrix

esp

anae

nsis

DSM

442

29Chl

orob

acul

um p

arvu

m N

CIB

832

7

Ferroglobus placidus DSM 10642

Bordetella petrii DSM 12804

Desulfatibacillum alkenivorans AK-01

Erwinia amylovora ATCC 49946

Krok

inob

acte

r sp.

4H

-3-7

-5

Candidatus Protochlamydia amoebophila UWE25

Dac

tylo

cocc

opsi

s sa

lina

PCC

830

5

Meiothermus ruber DSM 1279

Glu

cona

ceto

bact

er d

iazo

troph

icus

PAl 5

Chl

orob

ium

lim

icol

a D

SM 2

45

Pseu

doxa

ntho

mon

as s

uwon

ensis

426

75

Mes

orhi

zobi

um c

icer

i bio

var b

iser

rula

e W

SM12

71

Laribacter hongkongensis HLHK9

Azos

piril

lum

bra

sile

nse

Sp24

5

gam

ma

prot

eoba

cter

ium

HdN

1

Acidaminoco

ccus in

testini R

yC-M

R95

Burk

holde

ria sp

. CCG

E100

3

Para

cocc

us d

enitr

ifica

ns P

D12

22

Myc

opla

sma

leac

hii P

G50

Leuconostoc gasicomitatum LMG 18811

Desulfitobacterium dehalogenans ATCC 51507

Prop

ioni

bact

eriu

m a

cnes

KPA

1712

02

Clostridium thermocellum ATCC 27405

Cand

idat

us P

elag

ibac

ter u

biqu

e HT

CC10

62

Sino

rhiz

obiu

m fr

edii

NG

R23

4

Glaciec

ola ni

tratire

duce

ns FR

1064

Ther

mos

ynec

hoco

ccus

sp.

NK5

5

Polaro

monas

naph

thalen

ivora

ns C

J2

Rub

roba

cter

xyl

anop

hilu

s D

SM 9

941

Egge

rthel

la le

nta

DSM

224

3

Xant

hom

onas

alb

ilinea

ns G

PE P

C73

Campylobacter sp. 03-427

Xant

hom

onas

ory

zae

pv. o

ryza

e KA

CC 1

0331

Syne

choc

occu

s sp

. JA-

3-3A

b

Sang

uiba

cter

ked

diei

i DSM

105

42

Thio

alka

livib

rio s

ulfid

ophi

lus

HL-

EbG

r7

Lactobacillus sanfranciscensis TMW 1.1304

Dehalobacter sp. CF

Pre

vote

lla ru

min

icol

a 23

Alicyc

liphil

us de

nitrifi

cans

BC

Bacillus halodurans C-125

Desulfovibrio vulgaris str. Hildenborough

Paenibacillus alvei DSM 29

Ruminococcus torques L2-14

Cand

idatu

s Phy

topla

sma

austr

alien

se

Thermomicrobium roseum DSM 5159

Weissella koreensis KACC 15510

Sphi

ngob

ium

japo

nicu

m U

T26S

Eubacterium cellulosolvens 6

Yersinia pseudotuberculosis PB1/+

Lactococcus garvieae ATCC 49156

Novo

sphi

ngob

ium

aro

mat

icivo

rans

DSM

124

44

Cupria

vidus

taiw

anen

sis LM

G 1942

4

Acid

imic

robi

um fe

rroox

idan

s D

SM 1

0331

Amycoli

cicoc

cus s

ubfla

vus D

QS3-9A1

Coryn

ebac

terium

diph

theria

e HC02

Fusobacterium nucleatum subsp. vincentii 3_1_36A2

Achromobacter xylosoxidans A8

Sim

idui

a ag

ariv

oran

s SA

1 =

DSM

216

79

Myc

opla

sma

pneu

mon

iae

M12

9

Shewan

ella b

altica

OS18

5

Clostridium kluyveri DSM 555

Caldisphaera lagunensis DSM 15908

Alkaliphilus oremlandii OhILAs

Kosmotoga olearia TBF 19.5.1

Candidatus Accumulibacter phosphatis clade IIA str. UW-1

Gra

nulib

acte

r bet

hesd

ensis

CG

DNIH

1

Fran

cise

lla p

hilo

mira

gia

subs

p. p

hilo

mira

gia

ATC

C 2

5017

Myc

opla

sma

weny

onii s

tr. M

assa

chus

etts

Geobacillus kaustophilus HTA426

Nos

toc

sp. P

CC

712

0

Roseiflexus castenholzii DSM 13941

Thermoanaerobacter brockii subsp. finnii A

ko-1

Mycob

acter

ium in

trace

llular

e MOTT-6

4

Vulcanisaeta moutnovskia 768-28

Spha

eroc

haet

a ple

omor

pha

str. G

rape

s

Osc

illato

ria a

cum

inat

a PC

C 6

304

Polaro

monas

sp. J

S666

Tetragenococcus halophilus NBRC 12172

Enterobacter cloacae subsp. cloacae ATCC 13047

Leptospirillum ferriphilum ML-04

Bacillus selenitireducens MLS10

Candidatus Koribacter versatilis Ellin345

Finego

ldia m

agna

ATCC 2932

8

Periw

inkle

leaf y

ellow

ing p

hyto

plasm

a

Burk

holde

ria rh

izoxin

ica H

KI 4

54

Acid

iphi

lium

mul

tivor

um A

IU30

1

Veillonella parvu

la DSM 2008

Arth

roba

cter n

icotin

ovor

ans

Rho

doba

cter

cap

sula

tus

SB 1

003

Candidatus Nitrososphaera gargensis Ga9.2

Halobacteroides h

alobius DSM 5150

Nitratiruptor sp. SB155-2

Deinococcus radiodurans R1

Brachyspira hyodysenteriae WA1

Carboxydotherm

us hydrogenoform

ans Z-2901

Rhodo

cocc

us eq

ui 10

3S

Yersinia enterocolitica subsp. enterocolitica 8081

Ralsto

nia e

utro

pha

JMP1

34

Bacillus stratosphericus LAMA 585

Dehalococcoides sp. BAV1

Streptococcus infantis

Sulfurospirillum deleyianum DSM 6946

Thermacetogenium phaeum DSM 12270

Vibr

io c

hole

rae

O1

biov

ar E

l Tor

str.

N16

961

Thio

alka

livib

rio n

itrat

iredu

cens

DSM

147

87

Sacc

haro

polys

pora

ery

thra

ea N

RRL

2338

Geobacter lovleyi SZ

Geo

derm

atop

hilu

s ob

scur

us D

SM 4

3160

Xant

hom

onas

arb

orico

la p

v. pr

uni s

tr. C

FBP

5530

Natrinema pellirubrum DSM 15624

Burk

holde

ria p

hym

atum

STM

815

Thermoanaerobacte

r tengcongensis

MB4

Sulfolobus islandicus M.14.25

Acar

yoch

loris

mar

ina

MBI

C11

017

Oceanobacillus iheyensis HTE831

Rhod

ospi

rillu

m ru

brum

F11

Thermanaerovibrio acidaminovorans DSM 6589

Psyc

hrob

acte

r arc

ticus

273

-4

Candidatus Nitrosopumilus sp. AR2

Cel

lulo

phag

a ly

tica

DSM

748

9

Echi

nico

la v

ietn

amen

sis

DSM

175

26

Amyc

olat

opsis

orie

ntal

is HC

CB10

007

Met

hylo

bact

eriu

m p

opul

i BJ0

01

Thermodesulfatator indicus DSM 15286

Dehalococcoides sp. CBDB1

Fran

kia

sym

bion

t of D

atis

ca g

lom

erat

a

Streptococcus suis BM407

Herpetosiphon aurantiacus DSM 785

Mor

axell

a m

acac

ae 4

0822

5

Methanosarcina barkeri str. FusaroClostridium acetobutylicum ATCC 824

Mycob

acter

ium ul

ceran

s Agy

99

Brevibacillus brevis NBRC 100599

Campylobacter fetus subsp. fetus 82-40

Streptococcus constellatus subsp. pharyngis C1050

alph

a pr

oteo

bact

eriu

m H

IMB5

Micr

obac

teriu

m te

stace

um S

tLB0

37

Pelobacter carbinolicus DSM 2380

Vibr

io fu

rniss

ii NCT

C 11

218

halophilic archaeon DL31

Metallosphaera cuprina Ar-4

1123383

Nitrosomonas eutropha C91

Serratia sp. AS12

Actin

opla

nes

friul

iens

is D

SM 7

358

Burk

holde

ria xe

novo

rans

LB4

00

Thermanaerovibrio velox DSM 12556

Myc

opla

sma

putre

facie

ns K

S1

Mel

iorib

acte

r ros

eus

P3M

-2

Polym

orph

um g

ilvum

SL0

03B-

26A1

Met

hylo

phag

a sp

. JAM

1

Spiro

plasm

a sy

rphid

icola

EA-1

Candidatus Blochmannia floridanus

Pediococcus pentosaceus ATCC 25745

Mar

inom

onas

pos

idon

ica

IVIA

-Po-

181

Halorubrum lacusprofundi ATCC 49239

Polyn

ucleo

bacte

r nec

essa

rius s

ubsp

. asy

mbiotic

us Q

LW-P

1DMW

A-1

Cha

mae

siph

on m

inut

us P

CC

660

5

Caldicellulosiru

ptor owensensis

OL

Amphibacillus xylanus NBRC 15112

Leis

inge

ra m

ethy

loha

lidiv

oran

s D

SM 1

4336

Vibr

io fi

sche

ri ES

114

Dickeya paradisia

ca NCPPB 2511

Photorhabdus luminesce

ns subsp

. laumondii T

TO1

Sorangium cellulosum So ce56

Nitrosomonas europaea ATCC 19718

Pseu

dom

onas

den

itrific

ans A

TCC 1

3867

Mycob

acter

ium af

rican

um G

M0411

82

Sphi

ngom

onas

witt

ichii R

W1

Pseu

dom

onas

chlor

orap

his O

6

Mycob

acter

ium ab

sces

sus

Streptococcus dysgalactiae subsp. equisimilis GGS_124

Burk

holde

ria p

seud

omall

ei K9

6243

Vibr

io c

ampb

ellii

ATCC

BAA

-111

6

Hyperthermus butylicus DSM 5456

Candidatus Arthromitus sp. SFB-mouse-Japan

Helicobacter hepaticus ATCC 51449

Idiom

arina

loihi

ensis

L2TR

Candidatus Kinetoplastibacterium oncopeltii TCC290E

Myxococcus stipitatus DSM 14675

Keto

gulo

nici

geni

um v

ulga

re Y

25

Bacillus pseudofirmus OF4

Rubrivivax gelatinosus IL144

Coryne

bacte

rium gl

utamicu

m R

[Cell

vibrio

] gilv

us A

TCC

1312

7

Pyrolobus fumarii 1A

Chlamydophila pneumoniae CWL029

Singulisphaera acidiphila DSM 18658

Caldisericum exile AZM16c01

Coryn

ebac

terium

effic

iens Y

S-314

Stre

ptom

yces

bin

gche

ngge

nsis

BCW

-1

Ruminococcus albus 7

Sacc

haro

mon

ospo

ra c

yane

a NA

-134

Syne

choc

occu

s el

onga

tus

PCC

630

1

Trep

onem

a su

ccini

facie

ns D

SM 2

489

Fervidobacterium nodosum Rt17-B1

Cenarchaeum symbiosum A

Syne

choc

occu

s sp

. CC

9902

Methylobacillus flagellatus KT

Ric

ketts

ia a

ustra

lis s

tr. P

hillip

s

Mycob

acter

ium lif

landii

128F

XT

Cellu

lomon

as fla

vigen

a DS

M 2

0109

Burk

holde

ria a

mbif

aria

AMM

D

Methanotorris igneus Kol 5

Pseu

dana

baen

a sp

. PC

C 7

367

Methanocaldococcus jannaschii DSM 2661

Mar

icau

lis m

aris

MC

S10

Methanoregula boonei 6A8

Lactobacillus rhamnosus GG

Streptococcus infantarius subsp. infantarius CJ18

Ignisphaera aggregans DSM 17230

Phycisphaera mikurensis NBRC 102666

Campylobacter hominis ATCC BAA-381

Rhodopirellula baltica SH 1

Nitr

osoc

occu

s ha

loph

ilus

Nc

4

Ther

mos

ynec

hoco

ccus

elo

ngat

us B

P-1

Spiro

chae

ta afr

icana

DSM

8902

Chl

orob

ium

tepi

dum

TLS

Dehalococcoides sp. GT

Muc

ilagi

niba

cter

pal

udis

DSM

186

03

Planctomyces limnophilus DSM 3776

Stan

ieria

cya

nosp

haer

a PC

C 74

37

'Nos

toc

azol

lae'

708

Rahnella sp. Y

9602

Myxococcus fulvus HW-1

Cya

nobi

um g

raci

le P

CC

630

7

Chthonomonas calidirosea T49

Lactobacillus brevis ATCC 367

Krib

bella

flav

ida

DSM

178

36

Desulfotalea psychrophila LSv54

Clostridium stercorarium subsp. stercorarium DSM 8532

Streptococcus suis 05ZYH33

Trep

onem

a br

enna

bore

nse

DSM

121

68

Roseburia intestinalis XB6B4

Candidatus Korarchaeum cryptofilum OPF8

Trop

hery

ma

whip

plei

str.

Twi

st

Zobe

llia g

alac

tani

vora

ns

Erysipe

lothri

x rhu

siopa

thiae

str. F

ujisa

wa

Stre

ptom

yces

dav

awen

sis J

CM 4

913

Bifid

obac

teriu

m a

nim

alis

sub

sp. l

actis

AD

011

Can

dida

tus

Libe

ribac

ter a

siat

icus

str.

psy

62

Methanobrevibacter ruminantium M1

Chl

oroh

erpe

ton

thal

assi

um A

TCC

351

10

Chl

orob

ium

pha

eovi

brio

ides

DSM

265

Thiobacillus denitrificans ATCC 25259

Methanocella paludicola SANAE

Anab

aena

sp.

90

Arcobacter nitrofigilis DSM 7299

Vibr

io v

ulni

ficus

CM

CP6

Bordete

lla br

onch

isepti

ca 25

3

Rue

geria

sp.

TM

1040

Actin

obac

illus

suis

H91-

0380

Mycob

acter

ium sm

egmati

s str.

MC2 155

Sulfurospirillum barnesii SES-3

Shigella boydii Sb227

Bra

dyrh

izob

ium

japo

nicu

m U

SD

A 6

Dechloromonas aromatica RCB

Keto

gulo

nici

geni

um v

ulga

re W

SH-0

01

Nitrosomonas sp. Is79A3

Klebsiella va

riicola At-2

2

Trep

onem

a ca

ldaria

DSM

733

4

Methanohalobium evestigatum Z-7303

Thermotoga neapolitana DSM 4359

Ric

ketts

ia ja

poni

ca Y

H

Caldilinea aerophila DSM 14535 = NBRC 104270

Rhod

ospi

rillu

m p

hoto

met

ricum

DSM

122

Vulcanisaeta distributa DSM 14429

Thermodesulfovibrio yellowstonii DSM 11347

Syne

choc

occu

s sp

. CC

9311

Fran

cise

lla n

ovic

ida

U11

2

Stre

ptom

yces

nive

us N

CIM

B 11

891

Cap

nocy

toph

aga

ochr

acea

DSM

727

1

Aero

mon

as v

eron

ii B5

65

Myc

opla

sma

parv

um s

tr. In

dian

a

Haloarcula hispanica ATCC 33960

Natronobacterium gregoryi SP2

Geobacter bemidjiensis Bem

Thermofilum pendens Hrk 5

Dickeya ze

ae Ech1591

Alis

tipes

sha

hii W

AL

8301

Clostridiales genomosp. BVAB3 str. UPII9-5

Sino

rhiz

obiu

m s

p. M

14

Urea

plas

ma

parv

um s

erov

ar 3

str.

ATC

C 70

0970

Bordetella avium 197N

Fran

kia

sp. E

AN1p

ec

Candidatus Desulforudis audaxviator MP104C

Pand

orae

a sp.

RB-44

Lactobacillus casei ATCC 334

Turne

riella

parva

DSM 21

527

Candidatus Blochmannia chromaiodes str. 640

Brachy

spira

pilos

icoli 9

5/100

0

Veillonella sp

. OK1

Oenococcus kitaharae DSM 17330

Shewan

ella s

p. W

3-18-1

Clostridium cellulovorans 743B

Mycob

acter

ium ca

nettii

CIPT 14

0010

059

Caldicellulosiru

ptor hydrotherm

alis 108

Pyrococcus furiosus DSM 3638

Methanothermus fervidus DSM 2088

Bac

tero

ides

frag

ilis

YC

H46

Leuconostoc carnosum JB16

Altero

monas

sp. S

N2

Fibr

isom

a lim

i BU

Z 3

Taylorella equigenitalis MCE9

Clostridium clariflavum DSM 19732

Can

dida

tus

Azo

bact

eroi

des

pseu

dotri

chon

ymph

ae g

enom

ovar

. CFP

2

Acidobacterium capsulatum ATCC 51196

Desulfotomaculum kuznetsovii DSM 6115

Thermovirga lienii DSM 17291

Alka

lilim

nico

la e

hrlic

hii M

LHE-

1

Osc

illato

ria n

igro

-viri

dis

PCC

711

2

Paenibacillus terrae HPL-003

Neisseria lactamica 020-06

Beut

enbe

rgia

cav

erna

e DS

M 1

2333

Orie

ntia

tsut

suga

mus

hi s

tr. B

oryo

ng

Methylovorus glucosetrophus SIP3-4

Vibr

io c

incin

natie

nsis

Desulfurococcus kamchatkensis 1221nBu

rkho

lderia

phe

noliru

ptrix

BR34

59a

Buchn

era ap

hidico

la str

. APS (A

cyrth

osiph

on pi

sum)

Lactococcus lactis subsp. lactis Il1403

Akkermansia muciniphila ATCC BAA-835

Oct

adec

abac

ter a

ntar

ctic

us 3

07

Brevibacillus laterosporus LMG 15441

Pre

vote

lla in

term

edia

17

Din

oros

eoba

cter

shi

bae

DFL

12

Symbiobacterium thermophilum IAM 14863

Met

hylo

mic

robi

um a

lcal

iphi

lum

20Z

Bordetella parapertussis Bpp5

Actin

opla

nes

sp. S

E50/

110

Man

nhei

mia

hae

mol

ytic

a U

SDA-

ARS-

USM

ARC

-185

Bifid

obac

teriu

m lo

ngum

DJO

10A

Rhod

ospi

rillu

m c

ente

num

SW

Calditerrivibrio nitroreducens DSM 19672

Syntrophobotulus glycolicus DSM 8271

Rhodo

ferax

ferri

redu

cens

T118

Lepto

spira

inter

roga

ns se

rova

r Cop

enha

geni

str. F

iocru

z L1-

130

Burk

holde

ria g

lumae

BGR1

Thermus thermophilus HB8

Methanocaldococcus fervens AG86

Trep

onem

a pa

ralui

scun

iculi C

unicu

li A

Streptococcus equi subsp. zooepidemicus MGCS10565

Methylacidiphilum infernorum V4

Candidatus Methylomirabilis oxyfera

Pseud

oalte

romon

as ha

loplan

ktis T

AC125

Olig

otro

pha

carb

oxid

ovor

ans

OM

5

Rho

doba

cter

sph

aero

ides

2.4

.1

Arth

roba

cter p

hena

nthr

enivo

rans

Sph

e3

Para

cocc

us m

arcu

sii

Isop

teric

ola

varia

bilis

225

Serratia plymuthica 4Rx13

Hyp

hom

icro

bium

sp.

MC

1

Bacillus cytotoxicus NVH 391-98

Ruminococcus obeum A2-162

Mycob

acter

ium sp

. JDM60

1

Cand

idatu

s Phy

topla

sma

mali

Bru

cella

pin

nipe

dial

is B

2/94

Thermocrinis albus DSM 14484

Whe

at b

lue d

warf

phyto

plasm

a

Edwardsiella ta

rda EIB202

Mes

orhi

zobi

um a

ustra

licum

WSM

2073

Shewan

ella s

edim

inis H

AW-E

B3

Renib

acte

rium

salm

onina

rum

ATC

C 33

209

Crin

aliu

m e

pips

amm

um P

CC

933

3

Bifid

obac

teriu

m b

reve

AC

S-07

1-V-

Sch8

b

Salmonella enterica subsp. enterica serovar Typhi str. Ty2

Leifs

onia

xyli s

ubsp

. xyli

str.

CTCB

07

Pseu

domon

as m

ontei

lii SB

3101

Urea

plas

ma

urea

lyticu

m s

erov

ar 1

0 st

r. AT

CC 3

3699

Streptococcus parasanguinis ATCC 15912

Klebsiella pneumoniae su

bsp. pneumoniae MGH 78578

Staphylococcus aureus subsp. aureus N315

Can

dida

tus

Vesi

com

yoso

cius

oku

tani

i HA

Acine

toba

cter b

aum

annii

ATC

C 17

978

Methanococcus voltae A3

Ow

enw

eeks

ia h

ongk

onge

nsis

DS

M 1

7368

Chloroflexus sp. Y-400-fl

Cya

noth

ece

sp. P

CC

880

2

Candidatus Zinderia insecticola CARI

Candidatus Methanomethylophilus alvus Mx1201

Borre

lia cr

ocidu

rae s

tr. Ach

ema

Thermobaculum terrenum ATCC BAA-798

Ric

ketts

ia p

arke

ri st

r. Po

rtsm

outh

Proc

hlor

ococ

cus

mar

inus

sub

sp. m

arin

us s

tr. C

CMP1

375

Campylobacter lari RM2100

Desulfovibrio aespoeensis Aspo-2

Bar

tone

lla c

larr

idge

iae

73

Myc

opla

sma

hyop

neum

onia

e 23

2

Staphylothermus hellenicus DSM 12710

Methanolobus psychrophilus R15

Streptococcus mitis B6

Myc

opla

sma

hom

inis

ATCC

231

14

Clostridium sp. SY8519

Brenne

ria sp

. EniD

312

Erwinia piriflorinigrans CFBP 5888

Trep

onem

a az

oton

utric

ium Z

AS-9

Wol

bach

ia e

ndos

ymbi

ont o

f Onc

hoce

rca

oche

ngi

Hal

omon

as e

long

ata

DSM

258

1

Nocar

dia cy

riacig

eorg

ica G

UH-2

Mic

rocy

stis

aer

ugin

osa

NIE

S-84

3

Odo

ribac

ter s

plan

chni

cus

DS

M 2

2071

2

Beije

rinck

ia in

dica

sub

sp. i

ndic

a AT

CC

903

9

Candidatus Kinetoplastibacterium crithidii (ex Angomonas deanei ATCC 30255)

Sacc

haro

mon

ospo

ra g

lauc

a K6

2

Cand

idat

us P

elag

ibac

ter s

p. IM

CC90

63

Gem

inoc

ystis

her

dman

ii PC

C 6

308

Noc

ardi

opsi

s al

ba A

TCC

BAA

-216

5

Candidatus Kinetoplastibacterium blastocrithidii (ex Strigomonas culicis)

Mar

ivirg

a tra

ctuo

sa D

SM 4

126

Pelotomaculum thermopropionicum SI

Chlamydophila pecorum E58

Borre

lia sp

. SV1

Geobacillus sp. Y4.1MC1

Methanosaeta harundinacea 6Ac

Amyc

olat

opsis

med

iterra

nei U

32

Ehrli

chia

mur

is A

S145

Planococcus citreus

Comam

onas

testo

stero

ni CNB-2

Rahnella genomosp. 3

Campylobacter curvus 525.92

Rho

dom

icro

bium

van

niel

ii ATC

C 1

7100

Burk

holde

ria m

ultivo

rans

ATC

C 17

616

Psyc

hrob

acte

r cry

ohalo

lentis

K5

Hyp

hom

onas

nep

tuni

um A

TCC

154

44

Stre

ptom

yces

alb

us J

1074

Methanocella arvoryzae MRE50

Sten

otro

phom

onas

mal

toph

ilia K

279a

Marinithermus hydrothermalis DSM 14884

Phen

yloba

cter

ium

zuc

ineu

m H

LK1

Rhodo

cocc

us er

ythro

polis

PR4

Ehrli

chia

rum

inan

tium

str.

Wel

gevo

nden

Tann

erel

la fo

rsyt

hia

ATC

C 4

3037

Spha

eroc

haet

a co

ccoid

es D

SM 1

7374

Acine

toba

cter b

erez

iniae

Lactobacillus kefiranofaciens ZW3

Spiri

bact

er s

p. U

AH-S

P71

Acine

toba

cter c

alcoa

cetic

us P

HEA-

2

Sulfurimonas autotrophica DSM 16294

Candidatus Blochmannia vafer str. BVAF

Kocu

ria rh

izoph

ila D

C220

1

Clostridium botulinum A str. Hall

uncultured Termite group 1 bacterium phylotype Rs-D17

Borre

lia sp

ielman

ii A14

S

Natranaerobius t

hermophilus J

W/NM-WN-LF

Mes

oplas

ma

floru

m L

1

Streptococcus anginosus C238

Xant

hom

onas

axo

nopo

dis

pv. c

itrum

elo

F1

Stre

ptom

yces

coe

licol

or A

3(2)

Gordo

nia p

olyiso

pren

ivora

ns V

H2

Haloferax volcanii DS2

Lactobacillus ruminis ATCC 27782

Spiro

plasm

a dim

inutu

m C

UAS-

1

Escherichia coli UMN026

Fusobacterium nucleatum subsp. animalis 4_8

Natronomonas pharaonis DSM 2160

Acido

vora

x ebr

eus T

PSY

Mic

rom

onos

pora

aur

antia

ca A

TCC

270

29

Pseu

domon

as pr

otege

ns P

f-5

Syne

choc

occu

s sp

. RC

C30

7

Fran

kia

alni

AC

N14

a

Myc

opla

sma

croc

odyli

MP1

45

Dictyoglomus thermophilum H-6-12

Fran

cise

lla n

oatu

nens

is s

ubsp

. orie

ntal

is s

tr. T

oba

4

Pand

orae

a pno

menus

a 3kg

m

Chlamydophila abortus S26/3

Acidianus hospitalis W1

Elusimicrobium minutum Pei191

Thermofilum sp. 1910b

Myc

opla

sma

ovis

str.

Mich

igan

Thermoanaerobacter sp. X514

Prop

ioni

bact

eriu

m fr

eude

nrei

chii

subs

p. s

herm

anii

CIR

M-B

IA1

Streptococcus sp. I-P16

Sacc

haro

phag

us d

egra

dans

146

42

Geobacter uraniireducens Rf4

Psyc

hrof

lexu

s to

rqui

s AT

CC

700

755

Cyc

locl

astic

us s

p. P

1

Gallionella capsiferriformans ES-2

Ric

ketts

ia m

onta

nens

is s

tr. O

SU 8

5-93

0

Non

labe

ns d

okdo

nens

is D

SW-6

Oceanobacillus kimchii X50

Mycob

acter

ium m

arinu

m M

Shigella flexneri 2

a str. 301

Stre

ptom

yces

cat

tleya

NRR

L 80

57 =

DSM

464

88

Pela

giba

cter

ium

hal

otol

eran

s B2

Candidatus Nitrospira defluvii

Rhodo

cocc

us jo

stii R

HA1

Eubacterium rectale ATCC 33656

Bla

ttaba

cter

ium

sp.

(Mas

tote

rmes

dar

win

iens

is) s

tr. M

AD

AR

Helicobacter pylori 26695

Enterococcus hirae ATCC 9790

Mahella australiensis

50-1 BON

Enterococcus faecium DO

Thermoanaerobacter ita

licus A

b9

Vibr

io a

ngui

llaru

m 7

75

Halogeometricum borinquense DSM 11551

Anap

lasm

a ph

agoc

ytop

hilu

m s

tr. H

Z

Stre

ptom

yces

flav

ogris

eus A

TCC

3333

1

Lepto

spira

inter

rogan

s sero

var L

ai str

. 566

01

Cya

noth

ece

sp. P

CC

742

5

Nitrosopumilus maritimus SCM1

Spiri

bact

er s

alin

us M

19-4

0

Vario

vora

x par

adox

us S

110

Brachyspira intermedia PWS/A

Haloferax lucentense DSM 14919

Sulfuricurvum kujiense DSM 16994

Can

dida

tus

Car

sone

lla ru

ddii

PV

Metallosphaera sedula DSM 5348

Allo

chro

mat

ium

vin

osum

DSM

180

Sphi

ngop

yxis

alas

kens

is RB

2256

Acidaminoco

ccus f

ermentans D

SM 20731

Acid

iphi

lium

cry

ptum

JF-

5

Bar

tone

lla g

raha

mii

as4a

up

Arcobacter sp. L

Bacillus infantis NRRL B-14911

Listeria seeligeri serovar 1/2b str. SLCC3954

Methanococcus maripaludis S2

Burk

holde

ria sp

. CCG

E100

2

Faecalibacterium prausnitzii L2-6

Pseu

dom

onas

bra

ssica

cear

um su

bsp.

bra

ssica

cear

um N

FM42

1

Exiguobacterium sibiricum 255-15

Desulfitobacterium dichloroeliminans LMG P-21439

Myc

opla

sma

haem

ocan

is st

r. Illi

nois

Geobacter sp. M21

Shewan

ella v

iolac

ea D

SS12

Sulfolobus acidocaldarius DSM 639

Isosphaera pallida ATCC 43644

Candid

atus R

iesia

pedic

ulico

la USDA

Pseud

omon

as sy

nxan

tha B

G33R

Stre

ptom

yces

sp.

Sire

xAA-

E

Intra

spor

angi

um c

alvu

m D

SM 4

3043

Borre

lia re

curre

ntis A

1

Klebsiella pneumoniae su

bsp. pneumoniae HS11286

Chloroflexus aggregans DSM 9485

Escherichia coli str. K-12 substr. M

G1655

Shewan

ella b

altica

OS15

5

Laci

nutri

x sp

. 5H

-3-7

-4

Myr

oide

s od

orat

us D

SM 2

801

Pectobacte

rium wasabiae WPP163

Clostridium saccharoperbutylacetonicum N1-4(HMT)

Pedo

bact

er h

epar

inus

DSM

236

6

Proteus sp. 3

M

Clostridium novyi NT

Stre

ptom

yces

col

linus

Tu

365

Streptococcus uberis 0140J

Coryn

ebac

terium

resis

tens D

SM 4510

0

Natronococcus occultus SP4

Streptobacillus moniliformis DSM 12112

Lactobacillus sakei subsp. sakei 23K

Thermoanaerobacte

rium th

ermosacch

arolyticum DSM 571

Pseud

oalte

romon

as at

lantic

a T6c

Caul

obac

ter s

p. K

31

Archaeoglobus veneficus SNP6

Sulfuricella denitrificans skB26

Gayad

omon

as jo

obini

ege G

7

Desulfotomaculum carboxydivorans CO-1-SRB

Bru

cella

mel

itens

is b

v. 1

str.

16M

Syne

choc

ystis

sp.

PC

C 6

803

subs

tr. P

CC

-P

Pseu

doxa

ntho

mon

as s

padi

x BD

-a59

Leptothrix cholodnii SP-6

Rie

mer

ella

ana

tipes

tifer

ATC

C 1

1845

= D

SM

158

68

Stac

kebr

andt

ia n

assa

uens

is D

SM 4

4728

Myc

opla

sma

geni

taliu

m G

37

Thermococcus gammatolerans EJ3

Nitr

obac

ter h

ambu

rgen

sis

X14

Bacillus clausii KSM-K16

Coryn

ebac

terium

varia

bile D

SM 4470

2

Acid

ithio

baci

llus

ferri

vora

ns S

S3

Ralston

ia so

lanac

earu

m GMI10

00

Bacillus anthracis str. Sterne

Pseud

oalte

romon

as sp

. SM99

13

Ric

ketts

ia ty

phi s

tr. W

ilmin

gton

Pantoea ananatis LMG 20103

Syntrophus aciditrophicus SB

Archaeoglobus sulfaticallidus PM70-1

Yersinia pestis Pestoides F

Thermotoga thermarum DSM 5069

Rahnella aquatilis HX2

Methanohalophilus mahii DSM 5219

Che

lativ

oran

s sp

. BN

C1

Burk

holde

ria th

ailan

dens

is E2

64

Brev

undi

mon

as s

ubvib

rioid

es A

TCC

1526

4

Methanoplanus petrolearius DSM 11571

Haloarcula marismortui ATCC 43049

Vibr

io ha

rvey

i

Fran

cise

lla tu

lare

nsis

sub

sp. t

ular

ensi

s SC

HU

S4

Sulfurihydrogenibium sp. YO3AOP1

Flav

obac

teriu

m c

olum

nare

ATC

C 4

9512

Aero

mon

as h

ydro

phila

sub

sp. h

ydro

phila

ATC

C 7

966

Planctomyces brasiliensis DSM 5305

Rue

geria

pom

eroy

i DSS

-3

Pedo

bact

er s

alta

ns D

SM 1

2145

Borre

lia ga

rinii P

Bi

Enterobacter asburiae LF7a

Granulicella mallensis MP5ACTX8

Nia

stel

la k

oree

nsis

GR

20-1

0

secondary endosymbiont of Heteropsylla cubana

Ric

ketts

ia s

ibiri

ca 2

46

Pseu

dom

onas

syrin

gae

pv. p

hase

olico

la 14

48A

Wol

bach

ia e

ndos

ymbi

ont o

f Dro

soph

ila s

imul

ans

wH

a

Neo

ricke

ttsia

rist

icii

str.

Illin

ois

Vibr

io c

oral

liilyt

icus

Syne

choc

occu

s sp

. CC

9605

Spiro

plasm

a ap

is B3

1

Raoult

ella o

rnithi

nolyt

ica B6

Geobacter sulfurreducens PCA

Streptococcus iniae SF1

Streptococcus mutans UA159

Thermobacillus composti KWC4Su

lfito

bact

er g

uttif

orm

is

Sulfolobus tokodaii str. 7

Photorhabdus asym

biotica

Coryn

ebac

terium

genit

alium

ATCC 33

030

Petrotoga mobilis SJ95

Pyrobaculum sp. 1860

Bla

ttaba

cter

ium

sp.

(Cry

ptoc

ercu

s pu

nctu

latu

s) s

tr. C

pu

Streptococcus oralis Uo5

Chlamydia psittaci 6BC

Natronomonas moolapensis 8.8.11

Archaeoglobus fulgidus DSM 4304

Methylovorus sp. MP688

Taylorella asinigenitalis MCE3

Leuconostoc kimchii IMSNU 11154

Flav

obac

teriu

m b

ranc

hiop

hilu

m F

L-15

Man

nhei

mia

suc

cini

cipr

oduc

ens

MBE

L55E

Provide

ncia

snee

bia D

SM 1996

7

Aequ

oriv

ita s

ublit

hinc

ola

DSM

142

38

Bru

cella

sui

s 13

30

Nitratifractor salsuginis DSM 16511

Mar

ibac

ter s

p. H

TCC

2170

Helicobacter acinonychis str. Sheeba

Desulfurococcus mucosus DSM 2162

Tolu

mon

as a

uens

is D

SM 9

187

Libe

ribac

ter c

resc

ens

BT-1

Con

greg

ibac

ter l

itora

lis K

T71

Acidilobus saccharovorans 345-15

Thiomonas intermedia K12

Coryn

ebac

terium

terp

enota

bidum

Y-11

Can

dida

tus

Rut

hia

mag

nific

a st

r. C

m (C

alyp

toge

na m

agni

fica)

Astic

caca

ulis

exce

ntric

us C

B 48

Thermoanaerobacter pseudethanolicus ATCC 33223

Methanobrevibacter sp. AbM4

Pelobacter propionicus DSM 2379

Eubacterium siraeum V10Sc8a

Streptococcus pneumoniae R6

Sino

rhiz

obiu

m m

edic

ae W

SM41

9

Yersinia frederiksenii

Geobacillus sp. WCH70

Alca

nivo

rax

dies

elol

ei B

5

Staphylothermus marinus F1

Rhi

zobi

um tr

opic

i CIA

T 89

9

Anap

lasm

a ce

ntra

le s

tr. Is

rael

Can

dida

tus

Libe

ribac

ter s

olan

acea

rum

CLs

o-ZC

1

Bac

tero

ides

xyl

anis

olve

ns X

B1A

Tist

rella

mob

ilis K

A081

020-

065

Corallococcus coralloides DSM 2259

Burk

holde

ria vi

etna

mien

sis G

4

Chl

orob

ium

chl

oroc

hrom

atii

CaD

3

Ther

mom

onos

pora

cur

vata

DSM

431

83

Rhodo

cocc

us py

ridini

vora

ns S

B309

4

Thermoanaerobacte

rium sa

ccharolyti

cum JW/SL-YS485

Onion

yello

ws p

hyto

plasm

a OY

-MM

orax

ella

cata

rrhali

s RH4

Pseudogulbenkiania sp. NH8B

Desulfosporosinus meridiei DSM 13257

Roseburia hominis A2-183

Spiro

chae

ta the

rmop

hila D

SM 61

92

Mycob

acter

ium ch

ubue

nse N

BB4

Dehalogenimonas lykanthroporepellens BL-DC-9

Met

hylo

bact

eriu

m e

xtor

quen

s PA

1

Hyp

hom

icro

bium

den

itrifi

cans

ATC

C 5

1888

Pseu

dovi

brio

sp.

FO

-BEG

1

Noc

ardi

oide

s sp

. JS6

14

Thermotoga maritima MSB8

Acine

toba

cter s

p. A

DP1

Methanocaldococcus infernus ME

Methanothermococcus okinawensis IH1

Pseu

dom

onas

ent

omop

hila

L48

Lepto

spira

bifle

xa se

rovar

Patoc s

train

'Patoc 1

(Ames

)'

Shewan

ella s

p. ANA-3

Cand

idat

us M

ycop

lasm

a ha

emol

amae

str.

Pur

due

Bacillus anthracis str. Ames

Syne

choc

ystis

sp.

PC

C 6

803

subs

tr. G

T-I

Haem

ophi

lus

influ

enza

e Rd

KW

20

Burkholderiales bacterium JOSHI_001

Kyrpidia tusciae DSM 2912

Cap

nocy

toph

aga

cani

mor

sus

Cc5

Syntrophomonas wolfei subsp. wolfei str. Goettin

gen

Xant

hoba

cter

aut

otro

phic

us P

y2

Met

hylo

bact

eriu

m ra

diot

oler

ans

JCM

283

1

Leuconostoc citreum KM20

uncultured bacterium HHV216

Cand

idat

us E

ndol

issoc

linum

faul

kner

i L2

Candidatus Arthromitus sp. SFB-mouse-Yit

Lactobacillus amylovorus GRL 1112

Bacillus pseudomycoides DSM 12442

Waddlia chondrophila WSU 86-1044

Con

exib

acte

r woe

sei D

SM 1

4684

Methanococcoides burtonii DSM 6242

Leuconostoc sp. C2

Bru

cella

mic

roti

CC

M 4

915

Deinococcus deserti VCD115

Cyc

locl

astic

us z

ancl

es 7

-ME

Borre

lia ga

rinii N

MJW1

Methanothermobacter marburgensis str. Marburg

Cronobacter s

akaza

kii ATCC BAA-894

Methanococcus vannielii SB

Trep

onem

a pa

llidum

subs

p. p

allidu

m st

r. Nich

ols

Listeria welshimeri serovar 6b str. SLCC5334

Cupria

vidus

met

allidu

rans

CH34

Marino

bacte

r adh

aeren

s HP15

Desulfohalobium retbaense DSM 5692

Staphylococcus lugdunensis HKU09-01

Nitr

osoc

occu

s w

atso

nii C

-113

Sulfurimonas denitrificans DSM 1251

Cronobacter tu

ricensis

z3032

Mycob

acter

ium gi

lvum PYR-G

CK

Hae

mop

hilu

s so

mnu

s 12

9PT

Mag

neto

spiri

llum

mag

netic

um A

MB-

1

Erwinia pyrifoliae Ep1/96

Cand

idat

us M

ycop

lasm

a ha

emom

inut

um 'B

irmin

gham

1'

Desulfovibrio hydrothermalis AM13 = DSM 14728

Clostridium beijerinckii NCIMB 8052

Gor

doni

bact

er p

amel

aeae

7-1

0-1-

b

Coryne

bacte

rium gl

utamicu

m ATCC 13

032

Kita

sato

spor

a se

tae

KM-6

054

Methanobacterium sp. SWAN-1

Stre

ptos

pora

ngiu

m ro

seum

DSM

430

21

Pedo

bact

er s

p. B

G5

Acet

obac

ter p

aste

uria

nus

IFO

328

3-01

Aciduliprofundum boonei T469

Acine

toba

cter o

leivo

rans

DR1

Fibr

ella

aes

tuar

ina

BUZ

2

Picrophilus torridus DSM 9790

Mycob

acter

ium in

dicus

pran

ii MTCC 95

06

Spiro

som

a lin

gual

e D

SM 7

4

Salin

ibac

ter r

uber

DSM

138

55

Run

ella

slit

hyfo

rmis

DSM

195

94

Halanaerobium hydrogeniformans

Ric

ketts

ia c

onor

ii st

r. M

alis

h 7

Bar

tone

lla q

uint

ana

str.

Toul

ouse

Pyrobaculum arsenaticum DSM 13514

Paenibacillus mucilaginosus KNP414Bi

fidob

acte

rium

ado

lesc

entis

ATC

C 1

5703

Ethanoligenens harbinense YUAN-3

Candidatus Blochmannia pennsylvanicus str. BPEN

Lawsonia intracellularis PHE/MN1-00

Shimwellia

blattae DSM 4481 = NBRC 105725

Azos

piril

lum

lipo

feru

m 4

B

Igna

viba

cter

ium

alb

um J

CM

165

11

Gordo

nia b

ronc

hialis

DSM

432

47

Simkania negevensis Z

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Salmonella sp. M9397

Can

dida

tus

Uzi

nura

dia

spid

icol

a st

r. A

SN

ER

Pectobacte

rium sp. S

CC3193

Gei

tlerin

ema

sp. P

CC

740

7

Anaerolinea thermophila UNI-1

Cyc

loba

cter

ium

mar

inum

DSM

745

Thermoanaerobacter wiegelii R

t8.B1

Pusillimonas sp. T7-7

Lactobacillus crispatus ST1

Coryn

ebac

terium

urea

lyticu

m DSM 71

09

Bordete

lla pe

rtuss

is To

hama I

Segn

ilipar

us ro

tund

us D

SM 4

4985

Ric

ketts

ia p

row

azek

ii st

r. M

adrid

E

Streptococcus parauberis KCTC 11537

Shewan

ella p

ealea

na ATCC 70

0345

Met

hylo

cocc

us c

apsu

latu

s st

r. Ba

th

Persephonella marina EX-H1

Alis

tipes

fine

gold

ii D

SM

172

42

Melissococcus plutonius ATCC 35311

Fibrobacter succinogenes subsp. succinogenes S85

Mic

rom

onos

pora

sp.

L5

Thermus sp. CCB_US3_UF1

Bifid

obac

teriu

m b

ifidu

m P

RL2

010

Xenorhabdus nematophila ATCC 19061

Geobacter metallireducens GS-15

Marino

bacte

r sp.

BSs201

48

Cand

idatu

s Pun

iceisp

irillum

mar

inum

IMCC

1322

Mycob

acter

ium bo

vis AF21

22/97

Exiguobacterium antarcticum B7

Myc

opla

sma

bovis

PG

45

Methanoplanus limicola DSM 2279

Caldicellulosiru

ptor kronotsk

yensis 2002

Caldicellulosiru

ptor obsid

iansis OB47

Streptococcus thermophilus CNRZ1066

Nitrosospira multiformis ATCC 25196

Thio

alka

limic

robi

um c

yclic

um A

LM1

Lactobacillus acidophilus NCFM

Pseu

domon

as po

ae R

E*1-

1-14

Shigella dysenteriae Sd197

Listeria ivanovii subsp. ivanovii PAM 55

Psych

romon

as sp

. CNPT3

Nos

toc

punc

tifor

me

PCC

731

02

Pyrobaculum aerophilum str. IM2

Clav

ibacte

r mich

igane

nsis

subs

p. m

ichiga

nens

is NC

PPB

382

Ilyobacter polytropus DSM 2926

Cel

lvib

rio ja

poni

cus

Ued

a107

Aliiv

ibrio

sal

mon

icida

LFI

1238

Para

cocc

us a

estu

arii

Ther

mob

ispo

ra b

ispo

ra D

SM 4

3833

Acid

othe

rmus

cel

lulo

lytic

us 1

1B

Bacteriovorax marinus SJ

Syne

choc

occu

s sp

. WH

810

2

Sacc

haro

mon

ospo

ra v

iridi

s DS

M 4

3017

Past

eure

lla m

ulto

cida

sub

sp. m

ulto

cida

str.

Pm

70

Aero

mon

as a

quar

ioru

m A

AK1

Roth

ia de

ntoc

arios

a ATC

C 17

931

Sulfurihydrogenibium azorense Az-Fu1

Bac

tero

ides

thet

aiot

aom

icro

n V

PI-5

482

Thiomonas sp. 3As

secondary endosymbiont of Ctenarytaina eucalypti

Fran

kia

sp. C

cI3

Fusobacterium nucleatum subsp. nucleatum ATCC 25586

Clostridium diffic

ile 630

Coprococcus catus GD/7

Coprothermobacte

r proteolyti

cus DSM 5265

Riv

ular

ia s

p. P

CC

711

6

Cya

noba

cter

ium

sta

nier

i PC

C 7

202

Burk

holde

ria m

allei

SAVP

1

Terriglobus saanensis SP1PR4

Chlamydophila caviae GPIC

Sino

rhiz

obiu

m m

elilo

ti 10

21

Bordete

lla pe

rtuss

is 18

323

Glu

cona

ceto

bact

er h

anse

nii A

TCC

2376

9

Clostridium saccharolyticum WM1

Bra

dyrh

izob

ium

sp.

OR

S 2

78

Acid

ithio

baci

llus

cald

us S

M-1

Methanofollis liminatans DSM 4140

Aggr

egat

ibac

ter a

phro

philu

s N

J870

0

Streptococcus intermedius JTH08

Thermosphaera aggregans DSM 11486

Desulfurispirillum indicum S5

Met

hylo

bact

eriu

m e

xtor

quen

s C

M4

Actin

obac

illus

pleu

ropn

eum

onia

e se

rova

r 5b

str.

L20

Trep

onem

a pe

dis st

r. T

A4

Methanocella conradii HZ254

Mes

orhi

zobi

um o

ppor

tuni

stum

WSM

2075

Bacillus pumilus SAFR-032

Tric

hode

smiu

m e

ryth

raeu

m IM

S101

Desulfotomaculum reducens MI-1

uncultured bacterium HH1107

Aeropyrum camini SY1 = JCM 12091

Frat

euria

aur

antia

DSM

622

0

Desulfovibrio salexigens DSM 2638

Desulfobacterium autotrophicum HRM2

Chlamydia trachomatis D/UW-3/CX

Meiothermus silvanus DSM 9946

Pros

thec

ochl

oris

aes

tuar

ii D

SM 2

71

Cya

noth

ece

sp. P

CC

782

2

1123384

Rhodo

cocc

us op

acus

B4

Yersinia pestis CO92

Clostridium saccharobutylicum DSM 13864

Glo

eoca

psa

sp. P

CC

742

8

Thal

asso

lituu

s ol

eivo

rans

MIL

-1

Altero

monas

mac

leodii

str. '

Deep e

cotyp

e'

Thermoanaerobacter siderophilus SR4

Sphi

ngob

ium

fulig

inis

ATCC

275

51

Borre

lia va

laisia

na V

S116

Bacillus megaterium DSM 319St

rept

omyc

es ra

pam

ycin

icus

NRRL

549

1

Mag

neto

spiri

llum

gry

phisw

alde

nse

MSR

-1

Burk

holde

ria g

ladiol

i BSR

3

Dictyoglomus turgidum DSM 6724

Myc

opla

sma

haem

ofel

is st

r. La

ngfo

rd 1

Erwinia tasmaniensis Et1/99

Cyl

indr

ospe

rmum

sta

gnal

e PC

C 7

417

Methanosarcina acetivorans C2A

Chl

orob

ium

lute

olum

DSM

273

Thermodesulfobium narugense DSM 14796

Methanococcus aeolicus Nankai-3

Thermoanaerobacter sp

. X513

Geobacillus sp. Y412MC61

Denitrovibrio acetiphilus DSM 12809

Shewan

ella o

neide

nsis

MR-1

Xyle

lla fa

stid

iosa

9a5

c

Thermoproteus uzoniensis 768-20

Rhi

zobi

um e

tli C

FN 4

2

Listeria grayi

Escherichia coli IAI39

Anab

aena

cyl

indr

ica

PCC

712

2

Escherichia coli O104:H4 str. 2

011C-3493

Hydrogenobaculum sp. Y04AAS1

Pyrococcus sp. NA2

Nitr

obac

ter w

inog

rads

kyi N

b-25

5

Pectobacte

rium atrosepticu

m SCRI1043

Ehrli

chia

can

is s

tr. J

ake

Atop

obiu

m p

arvu

lum

DSM

204

69

Eryt

hrob

acte

r lito

ralis

HTC

C259

4

Hal

othe

ce s

p. P

CC

741

8

Ferri

monas

balea

rica D

SM 9799

Dic

helo

bact

er n

odos

us V

CS1

703A

Caul

obac

ter s

egni

s ATC

C 21

756

Kyto

cocc

us s

eden

tariu

s DS

M 2

0547

Shewan

ella d

enitri

fican

s OS21

7

Lactobacillus plantarum WCFS1

877455

Hal

orho

dosp

ira h

alop

hila

SL1

Rhi

zobi

um le

gum

inos

arum

bv.

vic

iae

3841

Burk

holde

ria ce

noce

pacia

HI2

424

Zym

omon

as m

obilis

sub

sp. m

obilis

ZM

4 =

ATCC

318

21

Ric

ketts

ia p

eaco

ckii

str.

Rus

tic

Streptococcus gordonii str. Challis substr. CH1

Chloroflexus aurantiacus J-10-fl

Myc

opla

sma

pulm

onis

UAB

CTIP

Mycob

acter

ium av

ium su

bsp.

parat

uberc

ulosis

K-10

Halobacterium sp. NRC-1

Serratia plymuthica AS9

Shewan

ella s

p. MR-4

Pre

vote

lla d

enta

lis D

SM

368

8

Natrialba magadii ATCC 43099

Ric

ketts

ia a

kari

str.

Har

tford

Gar

dner

ella

vag

inal

is 4

09-0

5

Archaeoglobus profundus DSM 5631

Candidatus Regiella insecticola LSR1

Alca

nivo

rax

bork

umen

sis

SK2

Yersinia pestis KIM10+

Chlamydia trachomatis 434/Bu

Cand

idat

us P

hoto

desm

us k

atop

tron

Akat

1

Clostridium acidurici 9a

Mycob

acter

ium va

nbaa

lenii P

YR-1

Bacillus cereus ATCC 14579

Sulfurimonas gotlandica GD1

Shewan

ella h

alifax

ensis

HAW

-EB4

Parachlamydia acanthamoebae UV-7

Oct

adec

abac

ter a

rctic

us 2

38

Methylibium petroleiphilum PM1

Aerococcus urinae ACS-120-V-Col10a

Sulfobacillus acidophilus TPY

Vario

vora

x sp.

DB1

Streptococcus gallolyticus UCN34

Pseu

dom

onas

men

docin

a ym

p

Cel

lulo

phag

a al

gico

la D

SM 1

4237

Lactobacillus reuteri DSM 20016

Truepera radiovictrix DSM 17093

Mycob

acter

ium le

prae T

N

Coryne

bacte

rium kr

oppe

nsted

tii DSM 44

385

Legi

onel

la p

neum

ophi

la s

ubsp

. pne

umop

hila

str.

Phi

lade

lphi

a 1

Thermococcus kodakarensis KOD1

Thermococcus sp. 4557

Borre

lia ga

rinii B

gVir

Leuconostoc gelidum JB7

Candidatus Nitrosoarchaeum limnia SFB1

Salmonella enterica subsp. enterica serovar Typhi str. CT18

Nocar

dia fa

rcini

ca IF

M 1

0152

Phae

obac

ter g

alla

ecie

nsis

DSM

173

95 =

CIP

105

210

Spiro

plasm

a ch

ryso

picola

DF-

1

Stre

ptom

yces

hyg

rosc

opicu

s su

bsp.

jingg

ange

nsis

TL01

Agro

bact

eriu

m fa

brum

str.

C58

Wol

bach

ia e

ndos

ymbi

ont s

train

TR

S of

Bru

gia

mal

ayi

Methanocaldococcus sp. FS406-22

Met

hylo

mon

as m

etha

nica

MC

09

Pola

ribac

ter s

p. M

ED15

2

Melissococcus plutonius DAT561

Verru

cosi

spor

a m

aris

AB-

18-0

32

Eubacterium limosum KIST612

Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293

Geobacillus thermoleovorans CCB_US3_UF5

Bla

ttaba

cter

ium

sp.

(Per

ipla

neta

am

eric

ana)

str.

BP

LAN

Desulfitobacterium hafniense Y51

Enterobacter lignolyticus SCF1

Dechlorosoma suillum PS

Brachy

spira

murd

ochii

DSM 12

563

Streptococcus agalactiae 2603V/R

Ric

ketts

ia fe

lis U

RR

WXC

al2

Salmonella bongori NCTC 12419

Stre

ptom

yces

ave

rmitil

is M

A-46

80

Arth

roba

cter a

rilaite

nsis

Re11

7

Flex

ibac

ter l

itora

lis D

SM 6

794

Granulicella tundricola MP5ACTX9

Methanocorpusculum labreanum Z

Bar

tone

lla b

acill

iform

is K

C58

3

Paenibacillus sp. JDR-2

Anap

lasm

a m

argi

nale

str.

St.

Mar

ies

Methanosalsum zhilinae DSM 4017

Bar

tone

lla a

ustra

lis A

ust/N

H1

Pyrococcus yayanosii CH1

Dya

doba

cter

ferm

enta

ns D

SM

180

53

Sideroxydans lithotrophicus ES-1

Stre

ptom

yces

sca

biei

87.

22

Heliobacterium modesticaldum Ice1

Oce

anim

onas

sp.

GK1

Selenomonas sputig

ena ATCC 35185

Azos

piril

lum

sp.

B51

0

Acidov

orax

aven

ae su

bsp.

aven

ae AT

CC 1986

0

Bacillus atrophaeus 1942

Mod

esto

bact

er m

arin

us

Gordo

nia sp

. KTR

9

Enterococcus faecalis V583

Azor

hizo

bium

cau

linod

ans

OR

S 57

1

Sulfolobus solfataricus P2

Flexistipes sinusarabici DSM 4947

Pantoea sp. A

t-9b

Pseu

dom

onas

sp. U

W4

Ros

eoba

cter

lito

ralis

Och

149

SpoVAASpoVABSpoVACSpoVADSpoVAEbSpoVAEaSpoVAF

SpoVFBSpoVV

Spo0ASpoVFA

Ramírez-Guadiana, et al., Fig. S4

Table S1. Bacillus subtilis strains used in this study. Strain Genotype Source Figure(s)

168 Wild-type (trpC2) Zeigler et al. 2008 1, 3, 5, S1, S2 and S3

BAM786 ∆gerAB::erm Koo et al., 2017

BDR3158 ∆gerAB Ramírez-Guadiana et al., 2017 1, S1 and S2

BDR3151 ΔspoVFA Ramírez-Guadiana et al., 2017 1, S1 and S2

BDR3154 ΔspoVV Ramírez-Guadiana et al., 2017 1, 5 and S2

BDR3205 ΔgerAB ΔspoVV Ramírez-Guadiana et al., 2017 1, 3, 5 and S2

BDR3205 ΔgerAB ΔspoVT Ramírez-Guadiana et al., 2017 1

BDR3206 ΔgerAB ΔspoVFA Ramírez-Guadiana et al., 2017 1, S1 and S2

BDR3205 ΔgerAB ΔytaF Ramírez-Guadiana et al., 2017 1

BDR3312 ∆spoVV::spec Ramírez-Guadiana et al., 2017

BDR3699 ∆spoVFB::erm This work S1

BDR3700 ∆gerAB ∆spoVFB::erm This work S1

BDR3414 ∆spoVV ∆gerA::spec This work 3

BDR3416 ycgO::cat This work

BDR3430 ycgO:: Phyperspank-spoVFAB (erm) This work 4 and S3

BDR3432 ycgO:: Phyperspank-spoVFAB (erm) amyE::PxylA-spoVV (spec) This work 4 and S3

BDR3449 spoVV-gfp (spec) This work

BDR3465 ycgO::PyeeK-optRBS-spoVV-gfp (erm) This work

BDR3466 ycgO::PspoVV-optRBS-spoVV-gfp (erm) This work

BDR3468 ∆spoVV ycgO::PyeeK-optRBS-spoVV-gfp (erm) This work

BDR3469 ∆spoVV ycgO::PspoVV-optRBS-spoVV-gfp (erm) This work 2

BDR3471 ∆gerA::spec ∆spoVV ycgO::PyeeK-optRBS-spoVV-gfp (erm) This work 3

BDR3458 ∆spoIIQ::kan This work

BDR3472 ∆spoIIQ::kan ∆spoVV ycgO::PspoVV-optRBS-spoVV-gfp (erm) This work 2

BCR1117 ∆spoIIIAH Laboratory stock

BDR3474 ∆spoIIIAH ∆spoVV::spec ycgO::PspoVV-optRBS-spoVV-gfp (erm) This work 2

BDR3507 ycgO::spoVV-gfp (spec) This work

BDR3527 ∆spoVV ycgO::spoVV-gfp (spec) This work 5

BDR3558 ∆spoVV ycgO::cat This work

BDR3632 ∆spoVV ycgO::spoVV-gfp (G96A) (spec) This work 5

BDR3562 ∆spoVV ycgO::spoVV-gfp (N97A) (spec) This work 5

BDR3615 ∆spoVV ycgO::spoVV-gfp (F141A) (spec) This work 5

BDR3563 ∆spoVV ycgO::spoVV-gfp (F302A) (spec) This work 5

BDR3564 ∆spoVV ycgO::spoVV-gfp (Q310A) (spec) This work 5

BDR3646 ∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (wt) (spec) This work 5

BDR3647 ∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (G96A) (spec) This work 5

BDR3648 ∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (N97A) (spec) This work 5

BDR3649 ∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (F141A) (spec) This work 5

BDR3650 ∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (F302A) (spec) This work 5

BDR3651 ∆gerAB::erm ∆spoVV ycgO::spoVV-gfp (Q310A) (spec) This work 5

All unmarked mutants are in-frame deletions generated by Cre-mediated recombination and contain a lox72 scar.

Table S2. Plasmids used in this study. Plasmid Description Source

pFR001 ycgO::Phyperspank-spoVFAB (erm) (amp) This work

pFR002 amyE::PxylA-spoVV (spec) (amp) This work

pFR008 ycgO::PyeeK-optRBS-spoVV-gfp (erm) (amp) This work

pFR009 ycgO::PspoVV-optRBS-spoVV-gfp (erm) (amp) This work

pFR011 ycgO::spoVV-gfp (spec) (amp) This work

pFR017 ycgO::spoVV(N97A)-gfp (spec) (amp) This work

pFR018 ycgO::spoVV(F302A)-gfp (spec) (amp) This work

pFR019 ycgO::spoVV(Q310A)-gfp (spec) (amp) This work

pFR024 ycgO::spoVV(QG96A)-gfp (spec) (amp) This work

pFR027 ycgO::spoVV(F141A)-gfp (spec) (amp) This work

Table S3. List of oligonucleotide primers used in this study. Primer Sequence oDR1247 ggACTAGTcaaagaaggtgaacgtttagaatg oDR1257 gccGCATGCtagaatatgcgggtgatgaccg oDR1250 gccGTCGACagcatcggggaggtaagaatg oDR1251 cgcGGATCCgccggaagtagagttggcgc oDR1262 ggcggcttcgttcttgctatg oDR1263 tttacttccggaaccctcgagtcctttttttgttccgtttgtcag oDR1264 ctcgagggttccggaagtaaa oDR1265 gttccatccgattatcacggg oDR1266 cccgtgataatcggatggaacggatgagtcaaaaccctcatcc oDR1267 agtggtgtggaacatgagagt oFR1 cttgtttccttccatcaggg oFR2 ttctgacctcgtttcccagc oFR3 agggcatggttgtgtatctgc oFR5 tgaatggtttctttattaggc oFR8 gtttcgcctcagggtatatg oFR11 gccGAATTCctgtaatcggtgtggaaggc oFR12 gccGCTAGCcataggttacagtccagagt oFR13 gccGCTAGCacataaggaggaactactatgaacttgtcgaagattaataca oFR15 gccGGATCCttatttgtatagttcatccatgccatgtgt oFR16 gccAAGCTTagcatgcacaagctgtctgac oFR17 gccGCTAGCtgattctttgtactaatatacgagcatagg oFR23 ggcACTAGTacaccgagatgagcaagccct oFR24 gccGGATCCttatttgtatagttcatccatgccatgtgt oFR32 atgggaatggcatcaggagccccagcgggcgcaaaactcac oFR33 gtgagttttgcgcccgctggggctcctgatgccattcccat oFR34 gtcagctttattcttggggcaagcggcttttccgtacaagc oFR36 ggcttttccgtacaagctgcagtggcaggtattttatcgga oFR40 gctatgggaatggcatcagcgaacccagcgggcgcaaaact oFR41 agttttgcgcccgctgggttcgctgatgccattcccatagc oFR46 ggcgctgttgccgtcggtgcgtttcaaaacgcatcactagg oFR47 cctagtgatgcgttttgaaacgcaccgacggcaacagcgcc oFR60 ggtcatgacattcgtgtccc oFR61 gctgctggcttggatcatcc oKO260 cgcgtacgctgcatatgtcta oKO0 ctcgttcatagtagttcctcc

Capital letters indicate the recognition sequence for restriction enzymes and underlines indicate mutated bases.