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siRNA
-24 hrs
Thymidine Release siRNA Thymidine
0
Release
-39 hrs -20 hrs -15 hrs-44 hrs 9 hrs
Mitosis
± Reversine 0.5 µMor AZ3146 2 µM
15 hrs
Wash-out15 hrs5 hrs 4 hrs 5 hrs 15 hrs
Bub1RNAi
Mad2RNAi
Rev0.5µM
AZ2µMCtrl
100 KDa
37
20
15
WB
33 hrs0
Drug Treatment
Bub1RNAi
Mad2RNAi
Rev0.5µM
AZ2µMCtrl
WB
57 hrs0
Drug Treatment
LC3-ILC3-II
Mad2
Actin
Bub1
B
A
100 KDa
37
siRNA Ctrl Bub1 Mad2
20
15 LC3-ILC3-II
Mad2
Actin
Bub1Ctrl Bub1 Mad2+ ZM447439
C
DIMR-90
15 KDa
37 Actin
Ctrl RevLC3-ILC3-II
Lymphoblastoid
15 KDa
100 Vinculin
Ctrl RevLC3-ILC3-II
LC3-ILC3-II
Actin
Ctrl Rev AZBJ-hTERT
15 KDa
37
E
F
Santaguida_Supplemental Figure 3
