Superb Image Quality for Best AnalysisLeica AM6000 and Hamilton Thorne IMSI-StrictTM software

The unique ICSI/IMSI system Leica AM6000 gets further

enhancement by Hamilton Thorne IMSI-StrictTM software.

Superb optics from Leica Microsystems provide extraordinary

high-magnification image quality of living spermatozoa. The

Hamilton Thorne software analyzes the sperms and provides a

categorization of the sperms.

BenefITS LeICA AM6000

› One control panel for microscope and manipulators – easy handling

› All systems functions are intelligently automated – reproducible images

› Micromanipulator movement automatically adjusted to microscope

magnification – fast and efficient control

› One supplier of electronics, optics and mechanics – customer care by one

company only

BenefITS HAMILTOn THOrne IMSI-STrICTTM SOfTwAre

› Objective classification of sperm removes inter-technician variation

› fast analysis with immediate results minimizes time from sample

preparation to ICSI procedure

› Allows standardization of user-selected sperm for ICSI

› no manual measurements of sperm required

› Save videos and snapshots for quality control and archiving

› Compatible with Leica DfC digital cameras

Copyright © by Leica Microsystems CMS GmbH, wetzlar, Germany, 2012.

Subject to modifications.

LeICA and the Leica Logo are registered trademarks of Leica Microsystems Ir GmbH.

LeICA AM6000 – THe UnIqUe SySTeM fOr MICrOMAnIpULATIOnTwo Competent Partners – One SolutionThe complete Leica AM6000 automated system is controlled from multifunction panels that cover not only micromanipulation, but also the most important microscope functions. This integrated system accelerates work processes and enhances efficiency.

In addition to all of the customary functions of eppendorf’s TransferMan® nK 2 micromanipulators, the Leica AM6000 panels also feature focus handwheels and function buttons for brightness, objective selection, and the magnification changer. Objectives can be associated with contrast methods, stored with the magnification changer, and recalled as required.

HAMILTOn THOrne IMSI-STrICTTM SOfTwAreStrict Criteria Brought to IMSIIMSI-StrictTM, developed on Leica DIC microscopes, is the only auto-mated software solution for live sperm morphology analysis under high magnification. IMSI-StrictTM combines Tygerberg Strict Criteria with motile sperm organelle morphology examination (MSOMe) and provides software-based categorization using head size, head shape, mid-piece, and number of vacuoles. Using these analyzed parameters, sperm are assigned to one of three categories – normal, sub-normal or abnormal (see images on the left side). IMSI-StrictTM software was developed using the standards of the Tygerberg Strict Criteria, in cooperation with prof. T.f. Kruger, Stellenbosch and Tygerberg Hospi-tal, South Africa and evaluated by prof. J. parinaud, Toulouse, france.

with IMSI-StrictTM, there are three methods of analysis from which to choose: 1) perform real-time morphology on the live, motile image, 2) take a snapshot of the live image and then initiate morphology analysis, or 3) record and save a video to use for analysis.

MOrpHOLOGy reSULTSnormal cells show two green checkmarks, plus a green sperm head circumference, green mid-piece designators, and no pink vacuole circles.

Sub-normal cells show one blue check-mark. The colors of the overlay and text will be either green or blue. Vacuoles (2 or less) will be designated by magenta circles.

Abnormal cells show one red X. either the color of at least one parameter will be red or 3 or more vacuoles will be designated by magenta circles.

The IMSI-StrictTM software includes the ability to turn off cell annotations on the main image to allow close visual inspection.

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