Summary Slide• Standards

– Science– Math

• Can you master the microscope?– Clues

• Types of Microscopes• Microscope Magnification

– Onion Skin Cells– Comparison Graph

• How to use the microscope– Steps

• Graphing Time– Answer

Science Standards

S5L3. Students will diagram and label parts of various cells (plant, animal, single-celled, multi-celled).a. Use magnifiers such as microscopes or hand lenses to observe cells and their structure.

S5CS3. Students will use tools and instruments for observing, measuring, and manipulating objects in scientific activities.

S5CS4. Students will use ideas of system, model, change, and scale in exploring scientific and technological matters.a. Observe and describe how parts influence one another in things with many parts.

Math Standards

M5P5. Students will represent mathematics in multiple ways. a. Create and use representations to organize, record, and communicate mathematical ideas.c. Use representations to model and interpret physical, social, and mathematical phenomena.

M5D1. Students will analyze graphs. a. Analyze data presented in a graph. b. Compare and contrast multiple graphic representations (circle graphs, line

graphs, bar graphs, etc.) for a single set of data and discuss the advantages/disadvantages of each.

M5D2. Students will collect, organize, and display data using the most appropriate graph.

Can you master the microscope?

Solve the mystery of the microscope by identifying parts of the microscope from clues provided. Click on the to check your deductive skills. Click on to go on to the next slide.

What type of microscope are we using?

Microscope Types

Compound Dissection

Scanning Electron

Microscope (SEM)

Transmission Electron

Microscope (TEM)

Compound

Clue # 1

• These two pieces are used to tilt, lift, support and carry the microscope carefully.

Clue # 2

• This part provides light to view the specimen.

Clue # 3

• This knob moves the body tube or stage (depending on your microscope) up and down.

Clue # 4

• This is the area to place your specimen for viewing.

Clue # 5

• This part of the microscope regulates the amount of light that enters through the stage opening.

Clue # 5

• These hold the slide in position.

Clue # 7

• This piece contains all of the objective lenses.

Microscope Magnification

Magnification Ocular lens

Total magnification

Scanning 4x 10x 40x

Low power

10x 10x 100x

High power

40x 10x 400x

Onion Skin Cells

Low-PowerHigh-Power

Look at the two pictures below. They are the same onion skin sample shown with the 10x and 40x objective lenses. Create a bar graph that represents the number of whole and partial cells visible at each magnification.

Onion Skin Cells Graph

27

60

102030

Number of Cells

10 x 40x

Magnification

Onion Skin Cells at Different Magnification

Clue # 8

• This is a knob that helps to focus on fine details of the specimen.

Clue # 9

• This is the piece that you look through to see the specimen.

Clue # 10

• These pieces enlarge the specimen to scanning, low, and high level of magnification.

Now we know all of the parts but how do we use

the microscope?

Step 1

• To carry the microscope grasp the microscopes arm with one hand.

• Place your other hand under the base. Place the microscope on a table with the arm toward you.

• Plug the microscope in and turn on the light source or adjust the mirror. Make sure that the cords do not obstruct walking paths.

Step 2

• Turn the coarse adjustment knob to lower the stage.

Step 3

• Revolve the nosepiece until the scanning objective lens clicks into place.

Step 4

• Adjust the diaphragm so that it lets in the most light possible.

• Look through the eyepiece to assure that all parts are correctly aligned.

Step 5

• Place a slide on the stage. Center the specimen over the opening on the stage. Use the stage clips to hold the slide in place.

Step 6

• Look at the stage from the side. Carefully turn the coarse adjustment knob to raise the stage until the scanning power objective almost touches the slide.

Step 7

• Looking through the eyepiece, VERY SLOWLY turn the coarse adjustment knob to lower the specimen until it comes into focus.

Step 8

• To switch to the low power objective lens, look at the microscope from the side. CAREFULLY revolve the nosepiece until the high-power objective lens clicks into place. Make sure the lens does not hit the slide.

• Look through the eyepiece and view your specimen. Adjust the fine adjustment knob to improve the focus.

Step 8

• To switch to the high power objective lens, look at the microscope from the side. CAREFULLY revolve the nosepiece until the high-power objective lens clicks into place. Make sure the lens does not hit the slide.

Step 9

• Looking through the eyepiece, turn the fine adjustment knob until the specimen comes into focus.

Step 10

• Diagram your observation through each of the objective lenses.

Wow…this is huge!

Graphing Time

• Travel around to each microscope and observe its specimen. Keep a tally of living, once living, and non living samples. Work with a partner to create a circle graph that represents the number and percentage of these three types.

Answer

Specimen Samples

once living, 7, 50%

living, 3, 21%

nonliving, 4, 29%

living

once living

nonliving

You are now a…

Click here to see if you are correct?

{After you are finished with the website , click the X in the upper right hand corner of the website.}Clue 1 Clue 3 Clue 5 Clue 7 Clue 9

Clue 2 Clue 4 Clue 6 Clue 8 Clue 10

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Step 1

Step 6

Step 2

Step 7

Step 3

Step 8

Step 4

Step 9

Step 5

Step 10

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scanning objective lens

diaphragm

light sourcestage clips

high-power objective lens

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low-power objective lens fine adjustment

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